Clip1 (NM_019765) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1110007I12Rik; 4631429H07Rik; AV017631; C81039; CLIP-170; Clip50; CLIP170; CYLN1; mKIAA4046
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR211959 representing NM_019765 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTATGCTAAAACCCAGCGGCCTGAAGGCCCCTACCAAGATCCTTAAGCCGGGAAGCACAGCCTTGA AGACGCCTGCTGCTGCTGCAGCTCCAGTGGAGAAGACAATACCCAGTGAGAAAGCCTCGGGCCCTCCATC CTCCGAGACCCAAGAGGAGTTTGTGGATGACTTTCGAGTTGGAGAACGTGTTTGGGTGAACGGGAATAAG CCCGGATTTATCCAGTTTCTTGGGGAAACTCAGTTCGCACCAGGCCAGTGGGCTGGGATTGTTTTAGATG AACCCATAGGGAAGAACGATGGCTCTGTGGCGGGAGTGCGGTATTTCCAGTGTGAACCTTTAAAGGGCAT ATTCACCCGACCTTCAAAGCTAACGAGGAAGGTGCAGGCAGAAGATGAAGCCAACGGCCTGCAGGCAGCT CCTGGGAGAACTGCTTCACCTCTGTCCACTGCTGCAGCTACCATGGTGTCTTCTTCTCCAGCCACTCCCT CAAATATTCCCCACAAACCGTCCCAGTCAACGGCAAAAGAACCTTCGGCTACACCTCAAATTAGCAACCT CACAAAAACTGCCAGTGAGTCGATCTCCAACCTTTCAGAGGCTGGCTCTGTCAAGAAGGGGGAGCGGGAG CTCAAGGTCGGAGACAGGGTGCTGGTTGGTGGCACTAAGGCTGGCGTAGTCCGCTTTCTTGGCGAGACCG ACTTTGCCAAGGGGGAGTGGTGTGGTGTGGAGTTAGACGAGCCTTTGGGGAAGAACGATGGTGCTGTGGC CGGAACAAGGTATTTTCAGTGTCAACCCAAATATGGATTGTTCGCTCCTGTCCACAAAGTGACAAAGATT GGCTTCCCGTCTACTACCCCAGCCAAAGCCAAAGCCGCCGCTGTGAGGCGAGTGATGGCCGCCACGCCCG CCAGCCTGAAGCGAAGCCCTTCTGCCTCCTCCCTCAGCTCCATGAGCTCCGTGGCCTCCTCTGTGAGCAG CAAACCCAGCCGGACAGGACTATTGACTGAAACCTCCTCCCGCTACGCCCGCAAGATCTCGGGCACCACT GCCCTCCAGGAGGCGCTGAAGGAGAAGCAGCAGCACATTGAGCAGCTGCTGGCTGAGCGGGACCTGGAGC GGGCCGAGGTGGCCAAGGCTACCAGCCACGTGGGGGAAATAGAGCAGGAGCTAGCCCTGGCCCGAGATGG GCATGACCAGCATGTCCTGGAACTAGAGGCCAAGATGGACCAGCTACGGACCATGGTGGAAGCTGCTGAC AGGGAGAAAGTGGAGCTGCTCAACCAGCTGGAAGAGGAGAAGAGGAAGGTTGAGGACCTTCAGTTCCGAG TTGAAGAAGAATCAATTACCAAAGGCGATCTTGAGGTGGCTACCGTCTCAGAAAAGTCCCGAATAATGGA ACTAGAAAAGGACCTAGCATTGAGAGCACAGGAAGTAGCTGAGCTCCGAAGAAGGCTAGAGTCCAGTAAA CCTCCTGGGGATGTGGATATGTCTCTCTCCCTTTTGCAAGAAATAAGTGCTTTGCAAGAAAAGCTAGAAG CCATCCATACTGACCACCAGGGCGAGATGACTTCTTTGAAGGAACACTTCGGAGCTCGAGAGGAGGCGTT TCAGAAGGAGATCAAGGCTCTGCACACGGCCACTGAGAAGCTTTCCAAAGAGAACGAGTCCCTGAGAAGC AAGCTCGACCACGCTAATAAGGAGAACTCCGATGTGATAGCTCTATGGAAGTCCAAACTGGAGACCGCCA TAGCATCCCACCAGCAGGCAATGGAGGAGCTGAAGGTGTCCTTTAGCAAAGGCATTGGAACGGACTCCGC TGAATTTGCTGAGTTAAAGACACAGATAGAGAGACTGAGACTAGATTACCAGCACGAAATAGAAAGTTTA CAGAGTAAACAGGACTCTGAGCGGTCTGCTCATGCTAAAGAGATGGAGACCATGCAGGCCAAGCTGATGA AGATCATTAAAGAGAAGGAGGACAGCCTGGAGGCCGTCAAAGCGAGACTGGACAGTGCAGAAGACCAGCA CCTAGTGGAAATGGAGGACACGCTAAACAAACTGCAGGAGGCGGAGATTAAGGTAAAGGAGCTAGAGGTT CTGCAGGCCAAGTACACTGAACAAAGCGAGGTCATTGGTAATTTTACATCGCAGCTCAGTGCTGTCAAAG AAAAGCTCTTGGATCTTGATGCGCTTCGGAAAGCCAATTCCGAAGGTAAACTGGAGCTCGAGACACTTAG ACAGCAGCTTGAGGGAGCTGAAAAGCAGATTAAAAACTTAGAGACTGAAAGGAACGCTGAAAGTAGCAAG GCTAATAGCATTACCAAAGAGCTCCAGGAGAAAGAGCTAGTGCTCACTGGTCTGCAGGACAGTTTGAATC AAGTCAATCAAGTGAAGGAGACTTTGGAGAAAGAACTTCAGACTTTGAAAGAAAAGTTTGCCAGTACTTC AGAGGAGGCGGTCTCTGCTCAGACAAGGATGCAAGATACCGTAAATAAACTGCACCAAAAGGAGGAACAG TTTAACGTGTTGTCGTCTGAACTGGAGAAGTTGAGAGAAAATTTAACAGACATGGAGGCAAAATTTAAAG AGAAGGATGATCGAGAAGATCAGCTGGTAAAGGCAAAGGAAAAATTAGAAAACGACATTGCAGAAATAAT GAAGATGTCAGGAGATAACTCTTCTCAGCTGACAAAGATGAATGATGAATTACGTCTGAAGGAAAGATCC GTGGAAGAACTCCAGCTCAAGCTTACAAAGGCCAATGAAAACGCCAGTTTTCTGCAGAAGAGTATTGGCG AAGTGACTCTTAAAGCTGAGCAGAGTCAGCAGCAAGCAGCCAGAAAACATGAAGAAGAAAAGAAAGAGTT GGAGGAAAAACTGTTGGAACTGGAAAAGAAGATGGAAACAAGCTACAACCAGTGTCAGGACCTGAAAGCT AAGTATGAAAAAGCCAGTTCTGAGACTAAAACAAAGCATGAAGAAATCCTGCAGAACCTCCAGAAGATGC TGGCGGACACAGAGGATAAGCTGAAGGCTGCTCAGGAGGCGAACAGAGATCTGATGCAGGACATGGAGGA GCTGAAAACACAGGCCGACAAAGCCAAAGCTGCTCAGACTGCAGAAGACGCCATGCAGATCATGGAACAG ATGACCAAAGAGAAAACAGAAACGCTGGCCTCCTTGGAGGACACCAAGCAAACAAATGCAAGACTACAGA ATGAATTGGACACACTTAAGGAAAACAACTTGAAAACTGTGGAAGAGCTGAACAAGTCAAAAGAACTGCT GAGTGTAGAGAACCAAAAAATGGAAGAATTCAAGAAAGAAATAGAAACCCTAAAGCAGGCAGCAGCTCAG AAGTCCCAGCAGCTTTCAGCACTGCAGGAAGAGAACGTCAAACTTGCCGAGGAGCTGGGGAGAACCCGGG ACGAAGTCACAAGTCACCAGAAGCTGGAAGAAGAACGATCTGTACTCAATAATCAGTTGTTAGAAATGAA AAAGAGAGAATCTGAGTTCAGAAAAGACGCCGATGAAGAGAAAGCCTCCTTGCAGAAGTCGATCAGCCTC ACCAGTGCCTTGCTCACAGAGAAGGACGCGGAGCTGGAGAAGCTGAGGAATGAGGTCACAGTGCTCAGGG GAGAGAATGCCACCGCCAAGTCCCTGCACTCAGTTGTGCAGACACTGGAGTCTGATAAGGTGAAGCTTGA GCTCAAGGTCAAAAACTTGGAACTTCAACTCAAGGAGAACAAGAGGCAGCTCAGCAGCTCCTCAGGTAAC ACTGATGCTCAGGCGGAAGAGGATGAGAGAGCCCAGGAGAGTCAGATTGATTTCCTCAACTCGGTAATAG TGGATCTTCAAAGAAAGAACCAAGACCTCAAGATGAAGGTGGAGATGATGTCTGAAGCTGCACTCAATGG CAACGGGGAAGACCTGAACAGTTATGACAGTGATGACCAGGAAAAGCAGTCCAAGAAGAAACCCCGCCTC TTCTGTGACATCTGTGACTGCTTTGACCTCCACGATACAGAGGACTGTCCCACCCAGGCACAGATGTCGG AAGATCCTCCCCATTCCACTCACCATGGCAGCCGGAGCGAGGAGCGGCCATACTGTGAGATCTGTGAGAT GTTTGGGCACTGGGCAACCAACTGCAATGACGACGAGACCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR211959 representing NM_019765 Red=Cloning site Green=Tags(s) MSMLKPSGLKAPTKILKPGSTALKTPAAAAAPVEKTIPSEKASGPPSSETQEEFVDDFRVGERVWVNGNK PGFIQFLGETQFAPGQWAGIVLDEPIGKNDGSVAGVRYFQCEPLKGIFTRPSKLTRKVQAEDEANGLQAA PGRTASPLSTAAATMVSSSPATPSNIPHKPSQSTAKEPSATPQISNLTKTASESISNLSEAGSVKKGERE LKVGDRVLVGGTKAGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCQPKYGLFAPVHKVTKI GFPSTTPAKAKAAAVRRVMAATPASLKRSPSASSLSSMSSVASSVSSKPSRTGLLTETSSRYARKISGTT ALQEALKEKQQHIEQLLAERDLERAEVAKATSHVGEIEQELALARDGHDQHVLELEAKMDQLRTMVEAAD REKVELLNQLEEEKRKVEDLQFRVEEESITKGDLEVATVSEKSRIMELEKDLALRAQEVAELRRRLESSK PPGDVDMSLSLLQEISALQEKLEAIHTDHQGEMTSLKEHFGAREEAFQKEIKALHTATEKLSKENESLRS KLDHANKENSDVIALWKSKLETAIASHQQAMEELKVSFSKGIGTDSAEFAELKTQIERLRLDYQHEIESL QSKQDSERSAHAKEMETMQAKLMKIIKEKEDSLEAVKARLDSAEDQHLVEMEDTLNKLQEAEIKVKELEV LQAKYTEQSEVIGNFTSQLSAVKEKLLDLDALRKANSEGKLELETLRQQLEGAEKQIKNLETERNAESSK ANSITKELQEKELVLTGLQDSLNQVNQVKETLEKELQTLKEKFASTSEEAVSAQTRMQDTVNKLHQKEEQ FNVLSSELEKLRENLTDMEAKFKEKDDREDQLVKAKEKLENDIAEIMKMSGDNSSQLTKMNDELRLKERS VEELQLKLTKANENASFLQKSIGEVTLKAEQSQQQAARKHEEEKKELEEKLLELEKKMETSYNQCQDLKA KYEKASSETKTKHEEILQNLQKMLADTEDKLKAAQEANRDLMQDMEELKTQADKAKAAQTAEDAMQIMEQ MTKEKTETLASLEDTKQTNARLQNELDTLKENNLKTVEELNKSKELLSVENQKMEEFKKEIETLKQAAAQ KSQQLSALQEENVKLAEELGRTRDEVTSHQKLEEERSVLNNQLLEMKKRESEFRKDADEEKASLQKSISL TSALLTEKDAELEKLRNEVTVLRGENATAKSLHSVVQTLESDKVKLELKVKNLELQLKENKRQLSSSSGN TDAQAEEDERAQESQIDFLNSVIVDLQRKNQDLKMKVEMMSEAALNGNGEDLNSYDSDDQEKQSKKKPRL FCDICDCFDLHDTEDCPTQAQMSEDPPHSTHHGSRSEERPYCEICEMFGHWATNCNDDETF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_019765
ORF Size	4173 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_019765.5, NP_062739.2
RefSeq Size	5877 bp
RefSeq ORF	4176 bp
Locus ID	56430
UniProt ID	Q922J3
MW	155.8 kDa
Gene Summary	Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking.[UniProtKB/Swiss-Prot Function]

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