Bcl9l (BC082304) Mouse Tagged ORF Clone

CAT#: MR211995

  • TrueORF®

Bcl9l (Myc-DDK-tagged) - Mouse B-cell CLL/lymphoma 9-like (cDNA clone MGC:90769 IMAGE:6856460)

ORF Plasmid: DDK tGFP



  "BC082304" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 9,968.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms MGC7014, DLNB11, BCL9-2, B9L
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR211995 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACCCAGAAAATAAACTGACCAATCATGGCAAGACAGGGAATGGAGGGGCCCAATCCCAGCACCAGA
ATGTGAACCAAGGACCCACCTGCAACCTGGGCTCCAAGGGCGTGGGGGCGGGGAGCCATGGGGCCAAGGC
CAACCAGATCTCACCTAGCAACTCAAGTCTGAAGAACCCCCAGGCAGGAGTGTCTCCTTTCAGCTCACTC
AAGGGCAAGGTGAAGCGCGAGAGGAGTGTGTCTGTGGACTCTGGAGAGCAGCGGGAAGCTGGGACTCCAT
CCCTCGATTCAGAGGCCAAAGAGGTGGCACCCCGGAGTAAACGGAGGTGTGTGCTGGAGCGGAAGCAGCC
GTACAGTGGGGACGAATGGTGCTCTGGGCCAGACAGCGAGGAGGACGACAAGCCCATTGCGGCCGCCCAC
AATTGTAATGTAGCAGACCCAGCCATGGTGACCCCACAGTTGGGTCCTGGCCAAACTGCCCAACTGCCCC
TCAGTGAGAGCAGTGCACCAGGCCCCCAACATGGCCCCCAGCCAGGCCTTCGGCCAGACGTTCCTGGGGG
TGGGGGTGGGGGCGTCCCAGGAAAGCCTCCGTCACAGTTCGTCTATGTCTTCACCACCCATCTGGCCAAC
ACAGCGGCAGAGGCAGTGCTGCAGGGCCGGGCAGAGTCCATCCTTGCCTACCACCAGCAGAATGTGCCTC
GGGCCAAGCTGGATCAGGCCCCTAAAGTGCCACCCACCCCAGAACCACTACCCCTGAATACGCCATCAGC
AGGTACACCACAGTCCCAGCCACCTCCTTTGCCACCGCCACCCCCAGCCCCTGGCAGTGCCCCTCCTGCT
CTGCCCCCGGAGGGGCCTCCTGAAGACACCAGTCAGGACCTGGCCCCCAACTCAGTGGGAGCTGCCAGTA
CAGGTGGTGGGACTGGGGGTACCCACCCTAACACCCCAACGGCTGCCACCGCTAACAACCCTCTGCCTCC
TGGAGGAGACCCTGGCAGTGCCCCTGGCTCCGCCCTATTGGGGGAGGCCACGCCCACCGGAAATGGGCAG
AGGAACCTGGTGGGCTCTGAGGGCCTGTCCAAAGAGCAGCTGGAGCACCGGGAGCGCTCCCTCCAGACAC
TGCGGGACATCGAGAGGCTGCTGCTCCGCAGTGGGGAGACTGAGCCCTTCCTCAAGGGGCCCCCGGGAGG
AGCTGGTGAGGGAGGCCCACCGGCACAAGCGCCCTCTGCTGCTCAACCGCCTCCCTCCGCCCCTCCTGGG
GGGCTGAAGAAGTATGAGGAGCCTCTGCAGTCAATGATCTCACAGACACAGAGCCTAGGAGGTCCCCCTC
TGGAGCATGAAGTGCCGGGGCACCCTCAGGGTGGAGACATGGGACAGCAAATGAACATGATGATGCAGAG
GCTGGGCCAGGACAGTCTGACGCCCGAGCAGGTGGCCTGGCGCAAACTGCAGGAAGAGTACTACGAGGAG
AAGCGGCGGAAAGAGGAGCAGATTGGATTGCACGGAGGCCGCCCTCTGCAGGACATGGTGGGAATGGGGG
GTATGATGGGGAGGGGGCCCCCACCTCCTTACCACAGCAAACCTGGGGATCAGTGGCCACCTGGAATGGG
TGCACAACTCCGAGGGCCTATGGATGTCCAAGATCCCATGCAGCTCCGACCTGGACCTCCCTTCCCTGGC
CCCCGTTTCCCAGGCAACCAGATGCAAAGGGTGCCCGGATTTGGAGGTATGCAGAGTATGCCCATGGAAG
TACCCATGAATGCCATGCAGAGACCTGTAAGGCCAGGCATGGCCTGGAATGAAGACTTGCCCCCTATTGG
GGGACCCAGCAACTTTGCCCAGAATGCCGTGCCCTACCCAGGTGGGCAGGGGGAGGCAGAGCGATTCATG
ACCCCTCGTGTCCGGGAGGAGCTGCTGAGGCACCAGTTGCTGGAGAAGCGGTCCATGGGCATGCAGCGTC
CCCTGGGCATGGCAGGTAGCGGCATGGGACAGAGCATGGAAATGGAACGGATGATACAGGCTCATCGACA
GATGGACCCTGCCATGTTCCCGGGACAGATGACTGGAGGAGATGGTCTCGCCGGCACACCCATGGGCATA
GAGTTTGGTGGAGGTCGGGGCCTCCTGAGCCCTCCAATGGGACAGTCTGGGCTGCGGGAGGTAGACCCGC
CTATGGGGCCAGGCAACCTCAACATGAACATGAATGTGAACATGAACATGAACATGAACCTGAATGTGCA
GATGACGCCCCAGCAGCAGATGCTGATGTCACAGAAGATGCGGGGCCCTGGAGACATGATGGGTCCTCAG
GGCCTCAGTCCCGAAGAGATGGCTCGGGTTCGGGCCCAGAACAGTAGTGGCATGATGGGGGGTCCGCAGA
AGATGCTCATGCCTTCACAGTTTCCCAACCAGGGCCAGCAGGGATTCTCTGGGGGCCAGGGACCTTACCA
AGCCATGCCCCAGGACATGGGCAACACTCCAGACATGTTCAGCCCTGATCAGAGTTCAGTGCCCATGGGC
ACTGTGGGCACTGCCCGGCTCAGCCATATGCCTCTGCCCCCTGCCTCCAATCCTCCTGGGTCTGTGCACT
TGGCCTCCAACAGGGGGCTAGGCAGGCGGCCTTCAGATCTCACCATCAGTATTAATCAGATGGGCTCACC
GGGCATGGGACATCTGAAGTCACCCACCCTTAGCCAGGTGCACTCCCCCCTGGTCACCTCACCCTCTGCC
AACCTCAAGTCACCCCAGACTCCCTCCCAGATGGTACCCTTGCCTTCTGCCAACCCACCGGGACCTCTCA
AGTCACCCCAGGTCCTCAGCTCTTCCCTCGGTGTGCGTTCACCCACTGGCTCACCCAGCAGGCTCAAGTC
TCCCTCCATGGCGGTGCCTTCTCCAGGCTGGGTCGCCTCTCCCAAGACAGCCATGCCTAGTCCTGGGGTC
TCCCAGAACAAGCAGCCACCTCTCAGCATAAACTCTTCCTCCACCCTGGGCAACGTGGAACAGGGTGCTC
TTCCACCTAGCGCACCCCGGAACAGCTCCTCCGCTCCTCCCGCCAACCCTTCCAGTGGCCTCATGAACCC
CAGCCTACCGTTCACATCCTCCCCAGACCCCACCCCTTCCCAGAACCCTCTGTCACTGATGATGTCTCAG
ATGTCCAAGTACGCCATGCCCAGCTCGACCCCGCTATACCACAACGCCATCAAGACCATCGCCACCTCAG
ATGACGAGCTGCTGCCTGACCGGCCCCTGCTACCCCCACCCCCACCACCGCAGGGCTCTGGGCCAGGTAT
CAGCAATAACCAGCCCAACCAGATGCACATGAACCCTGCTGCTGCCCAGAGCCCCATGGGCATGAACTTG
CCAGGCCAGCAGCCCCTGTCCCATGAGCCTCCCCCTACTATGTTGCCCTCCCCCACCCCTCTGGGGTCCA
ACATTCCACTGCATCCCAATGCACAGGGGACTGGGGGCTCTTCTCAAAACTCAATGATGATGGCCCCAGG
AGGCCCAGACTCCCTAAATGCCCCTTGTGGCCCTGTGCCCAGCTCCTCCCAGATGATGTCCTTCCCTCCT
CGGCTGCAGCAACCTCACGGTGCCATGGCCCCCACCGGGGCCGGGGGCCCAGGCCTGCAGCAGCACTACC
CTTCAGGCATGGCCCTGCCCCCCGAGGACCTGCCCACCCAGCCACCGGGTCCCATACCCCCCCAGCAGCA
CCTAATGGGCAAAGGCATGACTGGCCGCATGGGCGACGCATACCCACCCGGGGTGCTCCCTGGGGTGGCA
TCGGTACTGAATGACCCAGAGCTGAGTGAGGTGATCCGGCCCACCCCTACCGGCATTCCTGAGTTCGACT
TATCCAGGATCATCCCCTCTGAGAAACCAAGCAGCACCCTCCAGTACTTCCCCAAGAGCGAGAACCAGCC
CCCCAAGGCCCAGCCCCCCAATCTGCATCTCATGAACCTGCAGAACATGATGGCAGAGCAGACCCCGTCT
CGACCCCCCAACCTCCCGGGCCAACAGGGGGTCCAGCGGGGGCTCAGCATGTCCATGTGCCACCCTGGAC
AGATGTCCTTGCTGGGCAGGACAGGTGTGCCCCCACAACAGGGCATGGTGCCCCATGGCCTGCACCAGGG
GGTCATGTCCCCTCCACAAGGCCTCATGACCCAGCAGAATTTTATGCTGATGAAGCAGAGGGGTGTGGGG
GGCGAGGTCTACACCCAGCCTCCCCACATGCTCTCCCCACAGGGCTCCCTCATGGGCCCCCCACCCCAGC
AGAACCTCATGGTGTCCCACCCTCTGCGTCAGCGCAGTGTGTCTCTGGACAGCCAGATGGGCTACCTGCC
AACGCCGGGCAGCATGGCCAATCTACCCTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211995 protein sequence
Red=Cloning site Green=Tags(s)

MHPENKLTNHGKTGNGGAQSQHQNVNQGPTCNLGSKGVGAGSHGAKANQISPSNSSLKNPQAGVSPFSSL
KGKVKRERSVSVDSGEQREAGTPSLDSEAKEVAPRSKRRCVLERKQPYSGDEWCSGPDSEEDDKPIAAAH
NCNVADPAMVTPQLGPGQTAQLPLSESSAPGPQHGPQPGLRPDVPGGGGGGVPGKPPSQFVYVFTTHLAN
TAAEAVLQGRAESILAYHQQNVPRAKLDQAPKVPPTPEPLPLNTPSAGTPQSQPPPLPPPPPAPGSAPPA
LPPEGPPEDTSQDLAPNSVGAASTGGGTGGTHPNTPTAATANNPLPPGGDPGSAPGSALLGEATPTGNGQ
RNLVGSEGLSKEQLEHRERSLQTLRDIERLLLRSGETEPFLKGPPGGAGEGGPPAQAPSAAQPPPSAPPG
GLKKYEEPLQSMISQTQSLGGPPLEHEVPGHPQGGDMGQQMNMMMQRLGQDSLTPEQVAWRKLQEEYYEE
KRRKEEQIGLHGGRPLQDMVGMGGMMGRGPPPPYHSKPGDQWPPGMGAQLRGPMDVQDPMQLRPGPPFPG
PRFPGNQMQRVPGFGGMQSMPMEVPMNAMQRPVRPGMAWNEDLPPIGGPSNFAQNAVPYPGGQGEAERFM
TPRVREELLRHQLLEKRSMGMQRPLGMAGSGMGQSMEMERMIQAHRQMDPAMFPGQMTGGDGLAGTPMGI
EFGGGRGLLSPPMGQSGLREVDPPMGPGNLNMNMNVNMNMNMNLNVQMTPQQQMLMSQKMRGPGDMMGPQ
GLSPEEMARVRAQNSSGMMGGPQKMLMPSQFPNQGQQGFSGGQGPYQAMPQDMGNTPDMFSPDQSSVPMG
TVGTARLSHMPLPPASNPPGSVHLASNRGLGRRPSDLTISINQMGSPGMGHLKSPTLSQVHSPLVTSPSA
NLKSPQTPSQMVPLPSANPPGPLKSPQVLSSSLGVRSPTGSPSRLKSPSMAVPSPGWVASPKTAMPSPGV
SQNKQPPLSINSSSTLGNVEQGALPPSAPRNSSSAPPANPSSGLMNPSLPFTSSPDPTPSQNPLSLMMSQ
MSKYAMPSSTPLYHNAIKTIATSDDELLPDRPLLPPPPPPQGSGPGISNNQPNQMHMNPAAAQSPMGMNL
PGQQPLSHEPPPTMLPSPTPLGSNIPLHPNAQGTGGSSQNSMMMAPGGPDSLNAPCGPVPSSSQMMSFPP
RLQQPHGAMAPTGAGGPGLQQHYPSGMALPPEDLPTQPPGPIPPQQHLMGKGMTGRMGDAYPPGVLPGVA
SVLNDPELSEVIRPTPTGIPEFDLSRIIPSEKPSSTLQYFPKSENQPPKAQPPNLHLMNLQNMMAEQTPS
RPPNLPGQQGVQRGLSMSMCHPGQMSLLGRTGVPPQQGMVPHGLHQGVMSPPQGLMTQQNFMLMKQRGVG
GEVYTQPPHMLSPQGSLMGPPPQQNLMVSHPLRQRSVSLDSQMGYLPTPGSMANLPF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC082304
ORF Size 4371 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC082304, AAH82304
RefSeq Size 5472 bp
RefSeq ORF 4373 bp
Locus ID 80288
MW 152.6 kDa
Gene Summary Transcriptional regulator that acts as an activator. Promotes beta-catenin transcriptional activity. Plays a role in tumorigenesis. Enhances the neoplastic transforming activity of CTNNB1.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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