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Arap3 (NM_139206) Mouse Tagged ORF Clone
CAT＃: MR212026
- TrueORF®
Arap3 (Myc-DDK-tagged) - Mouse ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3 (Arap3), transcript variant 1
ORF Plasmid: tGFP
"NM_139206" in other vectors (4)
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CNY 10,520.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Centd; Centd3; cnt-d3; DRA; Drag1; E030006K04Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR212026 representing NM_139206
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCCCTCAGGACCTGGACATCGCTGTGTGGTTGGCCCTGGTGCATCTGGAGCAGTATGCGGACA CTTTCCGACGGCATGGCCTAGCTACAGCTGGTGCGGCCCAACACCTGGGCCACGAGGAGCTGAGGCACTT GGGCATCAGTGCTACAGGACACCGGAAACGCATCTTGCGCCTGTTGCGGGCAGGCTCTGCTGAGGGTTTC CTGGATTCTCATCTGGATAATACCATGGAGCCAACTCCCAGCCCAGCCCCTGATGCCCAACCCCCCAAGC CTGTGCCTAAGCCCAGGACGGTGTTTGGGCTCAGCAACCCTGCCACTGCCCAGAGGCCTGGACTGAGCCC AATCTTCTGGGATCCAGAAGTATCCAGGAACTCAGAGTGTACGCAAAGATCCTCTCCTCTCCTTCCTTCC TCCTCTGAACAGCCTTCAGTCCCGAATACCATGGAGATGATGCCTAATGCCATCTACTTTGGCCTGGACT TAAGAGGTCGGGCCCAGGCAGCTCAGGACGTGACTCCAGATAGTTCTCAGGCGACTGTCCCAACCCCTGC CTTCAGGCCCACAACAGGCACAGTGCACATCATGGATCCTGGTTGCCTGTACTACGGTGTCCAGCCTGTG GGGATCCCAGGAGCCTCTGACAGGAGAGACGGCAGGGGTGTCTGTCAGGAAAGGGCAGAACACAGACAGG ATCTGGAGACACGGGAGGATGCTGGTTATGCCAGCCTCGAGCTACCTGGAGACTCCATTCTTTCGCTACC CACCCAGGATGCAGAGACCAGTGATGACCTCATTTCGCCCTATGCCAGCTTCTCCTCCACAGCAGATCGT CCCGTGCCTCTGCTCAGTGGCTGGCTGGACAAGCTCTCACCTCAGGGAAACTATGTGTTCCAGAGACGTT TTGTGCAGTTCAATGGAAGGAGTCTAATGTACTTTGGCAGTGACAAGGACCCCTTCCCCAAAGGTGTGAT CCCTCTGACTGCCATCGAGATGACCCGAAGCAGCAAGGACAACAAATTCCAGGTCATTACCGGCCAGAGA GTATTTGTGTTCCGCACAGAGAGTGAGGCTCAGCGTGACCTATGGTGCTCCACGCTGCAATCCTGCCTGA AGGAACAGCGCCTCCTGGGCCACCCCCGGCCCCCTCACCCACCACGACCCCTCCGCACGGGCACGTTAGA GTTGCGTGGACACAAGGCCAAGGTGTTTGCTGCCTTGATCCCTGGAGAGCTGGCACTGTACAAGAGTGAG CAGGCCTTCTCACTGGGCATCGGGATCTGCTTCATTGAACTGCAAGGCTGCAGTGTCCGGGAGACCAAGA GTCGAAGCTTCGATCTGCTCACACCCCATCGCTGCTTCAGCTTCACAGCCGAGTCTGGGGGGGCTCGACA GAGCTGGGCAGCCGCTCTGCAAGAAGCAGTAACCGAGACACTGTCTGACTACGAGGTGGCTGAGAAGGTC TGGTCCAACCCGGCAAACCGGCATTGTGCGGACTGCAGGGCCTCCCGGCCGGATTGGGCTGCCGTCAACC TGGGGGTCGTCATCTGCAAGCAGTGTGCAGGTCAGCACAGGGCCCTGGGCTCTGGGATCTCCAAGGTGCA GAGCCTGAAGCTGGACACGAGTGTCTGGAGTAATGAGATAGTGCAGCTGTTCATTGTCCTGGGAAATGAT CGTGCCAATTGCTTCTGGGCAGGGGCACTTCCCCCAGGTGAAGGGCTGCATCCAGATTCAGCCCCTGGCC CTCGAGGAGAGTTCATCTCCCGAAAGTATAAGCTGGGTCTCTTCCGGAAGCCCCACCCTCGGCATCCAGA TCATAGCCAGCTTCTCCAGGCACTGTGTGCAGCTATGGCAGGACCCAACCTGCTCAAGAACATGGCCCAG CTTCTCTGTGTCGAAACCTCTGAGGGAGAGGAACCCTTGTCCCCTTCAGCCCTCAATGGCAGCTTGCTTA GCCTTCTACCTTCAGACTCCCCTGGAGTATACAATGAGGTGGTAGTGCCTGCCACTTACAGAGGTTTCCT GTACTGTGGCTCCATCAGCAACAAGGCTGGAGCCCCACCCCTTCGCAGGGGCCGGGATGCTCCTCCCCGC TTGTGGTGTGTGCTGGGAGCTGCCCTGGAAATGTTTGCATCAGAGAGCAGCCCTGAACCCCTCAGCCTTC TCCAGCCCCAGGATATTGTATGTCTGGGTGTAAGCCCCCCACCTGCTGATCCAGGTGACCTTGACAGGTT CCCCTTTTCCTTTGAACTCATCCTCACTGGGGGAAGAATCCAGCATTTTGCCACTGATGGAGCAGACAGT CTAGAGGCCTGGATCAGTGCAGTGGGCAAGTGGTTCTCCCCGCTCAGCTGCCACCAGCTGCTGGGCCCTG GGTTGCTGCGGATGGGTCGCCTGTGGCTACGGTCCCCCTCCCACGCAGGCCTGGCCCCTGGTCTCTGGCT GTCGGGCTTCGGCCTCCTTCGTGGTGATCATCTCTTCCTGTGCCCAGCGCCTGGCCCTGGTCCCCCTGCT CCGGAGGACATGGTGCATTTACGACGTCTACAGGAGATCAGTGTGGTCTCGGCAGCTGACACTCCAGACA AAAAGGAGCATTTGGTCCTTGTAGAGACAGGAAGGACCCTGTATTTGCAGGGGGAAGGCAGGCTAGACTT TGCAGCGTGGAACACGGCCATTGGAGGTGCTGCCGGAGGGGGTGGCACGGGCCTGCAGGAGCAGCAGATG AGCCGGGGAGACATCCCTATCATTGTGGACGCTTGCATCAGTTTTGTCACGCAGCATGGGCTCCGGCTGG AAGGTGTATATCGGAAAGGAGGCGCTCGAGCCCGCAGCCTGCGACTCTTGGCTGAGTTTAGGCGGGATGC CCGGTCAGTGAAGCTTAGACCACGGGAACACTTTGTAGAAGATGTTACTGACACACTCAAACGCTTCTTT CGAGAGCTTGATGACCCCGTGACCTCTGCTAGGTTGCTGCCTCGATGGAGAGAAGCTGCAGAGCTTTCCC AGAAGAATCAGCGCCTGGAGAAGTACAAAGAAGTGATTAGCTGCCTGCCTCGAGTCAACCGCAGAACACT GGCCACCCTCATCGGGCATCTGTACCGGGTGCAGAAGTGTGCGTCTCTAAACCAGATGTGCACGCGGAAC CTGGCCCTACTGTTTGCACCTAGTGTGTTCCAGACAGATGGGCGGGGAGAGCATGAGGTTCGGGTACTGC AGGAGCTCATCGATGGCTACATCTCTGTTTTTGATATTGACTCGGACCAGGCAGCTCAGATTGACTTGGA GGTCAGTCTCATTACCACCTGGAAGGATGTGCAGTTGTCCCAGGCTGGAGACCTCATCATGGAGGTTTAC ATAGAACAGCAGCTCCCGGACAACTGTGTCACCCTAAAGGTATCCCCAACACTGACTGCTGAGGAGTTGA CTAACCAGGTGCTGGAGATGCGAGGGGCGGCATCGGGGACAGACTTGTGGGTGACATTTGAAATTCTTGA ACACGGAGAGCTTGAGAGGCCACTGCACCCCAAAGAAAAGGTTCTAGAGCAGGCCTTGCAGTGGTGCCAG CTTCCAGAGCCCTGCTCAGCCTCTCTGCTTCTGAGAAAAGTCTCTATGGCACACGCTGGCTGCCTCTTCA CAGGAGTCCGGCGTGAGAGCCCACGAGTGGGGCTGCTACGGTGTCGTGAGGAGCCACCTCGATTGCTGGG AAACCGTTTCCAGGAGAGGTTCTTTTTGGTGCGTGGCCGATGCCTGCTGCTGCTCAAGGAGAAGAAGAGC TCTAAGCCAGAACGGGAGTGGTCTCTGGAAGGTGCCAAGGTCTACCTGGGAATCCGCAAGAAGTTAAAAC CTCCAACACTGTGGGGTTTCACATTAATATTGGAAAAGATGCACCTCTGTCTGTCCTGCATGGATGAGGA GGAGATGTGGGACTGGACCACAAGCATCCTTAAAGCCCAGCATGATGACCAACAGTCCGTGGTTTTACGT CGCCGTTCCTCCTCTGACCTTGCTCGTCAGAAGTTTGGCACTATGCCCTTGCTGCCCATCCGAGGGGATG ACAGTGGAGCCACCCTTCTCTCTGCCAATCAGACCCTGCGGCGACTACACAACCGGAGGACCTTATCCAT GTTCTTTCCAATGAAGTCACCCCAGGGATCTGTGGAAGAGCAAGATGAGCTGGAGGAGCCTGTGTATGAA GAGCCAGTATATGAGGAGGTCGGGGCCTTCCCTGAGCTTACCAAGGACACGACCTTCTCTTCCACATGGG AGTGGTCAGCCAAGTCAGATCCATCCCTTACCAGCCAGAGGTCCTTTGATCAACCCCCCCTCTCCAAGGC AAGCATGTTGGGCCACGAAGAGAGGATACCTGACCCTCCTCCGGGCCCCCCTTCAAAAAGCAGTTCTCAG GCACGAGGGTCCCTAGAGGAACAGCTGCTTCAGGAACTCAACAACCTCATCCTGAGGAAAGGAGAGCCTG CCTCGTGCCCAGAAAGCTCTTCCCAGCCCACCAGTCCCCAAGCCCCAAGCCCCACCAGCCTTCCAACACC GACCCCCAGCTTACCCACCCAACCGCCCTGCACTTCCAATCCACCCTCCAGCCAGCCTCTCACA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR212026 representing NM_139206
Red=Cloning site Green=Tags(s) MAAPQDLDIAVWLALVHLEQYADTFRRHGLATAGAAQHLGHEELRHLGISATGHRKRILRLLRAGSAEGF LDSHLDNTMEPTPSPAPDAQPPKPVPKPRTVFGLSNPATAQRPGLSPIFWDPEVSRNSECTQRSSPLLPS SSEQPSVPNTMEMMPNAIYFGLDLRGRAQAAQDVTPDSSQATVPTPAFRPTTGTVHIMDPGCLYYGVQPV GIPGASDRRDGRGVCQERAEHRQDLETREDAGYASLELPGDSILSLPTQDAETSDDLISPYASFSSTADR PVPLLSGWLDKLSPQGNYVFQRRFVQFNGRSLMYFGSDKDPFPKGVIPLTAIEMTRSSKDNKFQVITGQR VFVFRTESEAQRDLWCSTLQSCLKEQRLLGHPRPPHPPRPLRTGTLELRGHKAKVFAALIPGELALYKSE QAFSLGIGICFIELQGCSVRETKSRSFDLLTPHRCFSFTAESGGARQSWAAALQEAVTETLSDYEVAEKV WSNPANRHCADCRASRPDWAAVNLGVVICKQCAGQHRALGSGISKVQSLKLDTSVWSNEIVQLFIVLGND RANCFWAGALPPGEGLHPDSAPGPRGEFISRKYKLGLFRKPHPRHPDHSQLLQALCAAMAGPNLLKNMAQ LLCVETSEGEEPLSPSALNGSLLSLLPSDSPGVYNEVVVPATYRGFLYCGSISNKAGAPPLRRGRDAPPR LWCVLGAALEMFASESSPEPLSLLQPQDIVCLGVSPPPADPGDLDRFPFSFELILTGGRIQHFATDGADS LEAWISAVGKWFSPLSCHQLLGPGLLRMGRLWLRSPSHAGLAPGLWLSGFGLLRGDHLFLCPAPGPGPPA PEDMVHLRRLQEISVVSAADTPDKKEHLVLVETGRTLYLQGEGRLDFAAWNTAIGGAAGGGGTGLQEQQM SRGDIPIIVDACISFVTQHGLRLEGVYRKGGARARSLRLLAEFRRDARSVKLRPREHFVEDVTDTLKRFF RELDDPVTSARLLPRWREAAELSQKNQRLEKYKEVISCLPRVNRRTLATLIGHLYRVQKCASLNQMCTRN LALLFAPSVFQTDGRGEHEVRVLQELIDGYISVFDIDSDQAAQIDLEVSLITTWKDVQLSQAGDLIMEVY IEQQLPDNCVTLKVSPTLTAEELTNQVLEMRGAASGTDLWVTFEILEHGELERPLHPKEKVLEQALQWCQ LPEPCSASLLLRKVSMAHAGCLFTGVRRESPRVGLLRCREEPPRLLGNRFQERFFLVRGRCLLLLKEKKS SKPEREWSLEGAKVYLGIRKKLKPPTLWGFTLILEKMHLCLSCMDEEEMWDWTTSILKAQHDDQQSVVLR RRSSSDLARQKFGTMPLLPIRGDDSGATLLSANQTLRRLHNRRTLSMFFPMKSPQGSVEEQDELEEPVYE EPVYEEVGAFPELTKDTTFSSTWEWSAKSDPSLTSQRSFDQPPLSKASMLGHEERIPDPPPGPPSKSSSQ ARGSLEEQLLQELNNLILRKGEPASCPESSSQPTSPQAPSPTSLPTPTPSLPTQPPCTSNPPSSQPLT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_139206 |
| ORF Size | 4614 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_139206.2, NP_631945.2 |
| RefSeq Size | 5266 bp |
| RefSeq ORF | 4617 bp |
| Locus ID | 106952 |
| UniProt ID | Q8R5G7 |
| MW | 170.2 kDa |
| Gene Summary | This gene encodes a phosphoinositide binding protein containing ARF-GAP, RHO-GAP, RAS-associating, and pleckstrin homology domains. The ARF-GAP and RHO-GAP domains cooperate in mediating rearrangements in the cell cytoskeleton and cell shape. It is a specific PtdIns(3,4,5)P3/PtdIns(3,4)P2-stimulated Arf6-GAP protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224659 | Arap3 (untagged) - Mouse ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3 (Arap3), transcript variant 1, (10ug) |
CNY 19,860.00 |
|
| MG212026 | Arap3 (tGFP-tagged) - Mouse centaurin, delta 3 (Centd3) |
CNY 13,300.00 |
|
| MR212026L3 | Lenti ORF clone of Arap3 (Myc-DDK-tagged) - Mouse ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3 (Arap3), transcript variant 1 |
CNY 13,970.00 |
|
| MR212026L4 | Lenti ORF clone of Arap3 (mGFP-tagged) - Mouse ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3 (Arap3), transcript variant 1 |
CNY 13,970.00 |
