Arhgap21 (NM_001081364) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	5530401C11Rik; AA416458; ARHGAP10
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR212100 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCCACACATTGGACTGGTCTGCCTGAGGAAGATGGTGACAAACTCAAGGCCTGTGGGGCGGCCT CTGCCTGTGAGGTGTCAAAAAACAAAGATGGAAAAGACCAAGGTGAACCTGTGTCACCGTCTGAAGATGA GCCATTCTCCTGGCCAGGTCCCAAAACAGTCATGCTGAAGAGAACATCTCAAGGGTTTGGTTTCACATTA CGACACTTCATCGTTTATCCCCCAGAGTCTGCAATCCAGTTTTCATATAAGGATGAAGAGAATGGAAACA GAGGAGGAAAGCAAAGGAACCGATTGGAGCCGATGGATACCATATTTGTTAAACAAGTGAAGGAAGGAGG GCCGGCCTTCGAGGCTGGCTTATGCACAGGTGACAGAATTATAAAAGTCAATGGAGAAAGTGTCATAGGA AAGACCTATTCCCAAGTAATTGCTCTCATTCAGAACAGTGACACCACCCTGGAGCTCAGTGTAATGCCCA AGGATGAAGACATTCTCCAAGTGGCATATTCTCAAGATGCATACCTGAAAGGCAATGAAGCTTACAGCGG CAATGCCCGTAATATACCTGAACCTCCACCAGTTTGCTATCCCTGGTTGCCATCGACTCCTTCAGCTACT GCACAGCCGGTGGAAACATGCCCTCCTGATTCATTACCGAACAAACAGCAAACCAGTGCACCAGTCCTCA CACAACCTGGCAGGGCCTATAGAATGGAAATACAAGTGCCTCCGTCACCAACAGATGTTGCAAAGTCAAA CACAGCAGTGTGTGTTTGCAATGAGAGTGTGAGGACCGTCATTGTGCCTTCTGAGAAGGTGGTAGACTTG CTAGCCAATAGAAACAACCCTTCAGGTCCTTCACATAGAACTGAAGAGGTAAGGTATGGTGTAAATGAGC AGGCCTCTACAAAAGCAGCATCGAGAACAACGTCACCGGCATCAGTTCCCACTGCCCATCTGATTCATCA GACCACAGGCTCCAGATCCTTAGAACCTTCTGGAATTTTACTTAAATCTGGAAATTACAGTGGACATTCA GAAGGAATCTCAAGCAGTAGATCACAAGCCGTGGATTCCCCTCCTGTGTCTGTCAATCACTACTCTGCAA ACTCCCATCAGCACATAGACTGGAAAAATTATAAGACTTACAAAGAATACATTGATAATAGGAGATTGCA CATAGGTTGTCGGACAATCCAGGAAAGACTGGACAGCTTACGAGCTGCATCTCAGAGCGCAGCAGACTAT AACCAGGTGGTACCCACCCGCACTACTCTGCAGGTACGACGTCGAAGCACCTCTCATGACCGAGTGCCCC AGTCTGTTCAGATCCGGCAGCGCAGTGTATCTCAGGAAAGACTGGAAGATTCTGTGCTGATGAAGTATTG CCCAAGGAGCGCGTCTCAAGGAGCGCTGACCTCACCGCCTGTTAGCTTCAATAATCATAGAACTCGTTCG TGGGATTACATAGAGGGACAGACGGAAGCCACAGCCACTGTCAATTCTGAAAGTCAGATACCTGACTCAA ATGGGGAACGAAAACAGACTTACAAGTGGAGTGGCTTCACTGAGCAGGATGATAGACGCGGTATTCACGA AAGACCCAGGCAGCAAGAAATGCACAAGCCTTTCCGAGGCTCAAATTTGACTGTGGCTCCCGTTGTTAAT TCTGATAACAGGCGCCTGGTTGGGAGAGGGGTAGGACCTGTCTCACAGTTTAAAAAAATCCCGCCGGACC TAAGACCACCACATTCCAACAGAAATTTTCCAACCACGACTGGGGTGTCACTTCAACGGGGTATTGCACA AGACAGGTCCCCACTCGTGAAAGTCCGAAGTAATTCTCTGAAAGTGCCACCTCCACCTGTGTCAAAACCG TCCTTCAGCCAGCACTCACTGGCTTCCATGAAAGACCAAAGGCCTGTCAATCATCTGCATCAACACAGTG TGCTGAGTCAGCAGACGCAGTTCAGATCCGAAAGCACCTTCGAGCACCAGCTGGAGACTGAGGTATCCTC GTGTCTACCAGGAACTTCTGCCAAGACCAGTCCACAGCTCAGTGAGAATTTGGGAACATCAGACTTAGAA CTACCTGCCATCCCAAGGAATGGAGATATAAACTTACAGGAGGCTGAAATTCAGCAGCCAGATGTGCTAG ATAATAAAGAATCTGTCATTCTAAGAGAGAAACCTCAGTCTGGCCGCCAGACGCCACAGCCTTTAAGACA TCAGTCTTACATCCTGGCAGTGAATGACCAGGAGACCGGGTCAGACACCACCTGCTGGCTGCCAAACGAT GCACGCAGAGAGGTCCATATAAAAAGAATGGAGGAGAGGAAAGCTTCAAGCACCAGCCCTCCTGGTGATT CCCTGGCATCCATCCCTTTTATAGATGAGCCAACCAGTCCTAGCATCGACCACGAGATTGCACATATTCC TGCATCTGCTGTCATCTCCGCCTCTACTGCCCACGTCCCCTCCATTGCAACTGTTCCCCCCAGCCTCACG ACCTCTGCTCCTTTGATTCGACGTCAGCTCTCCCACGACCAAGAATCTGTTGGACCTCCCAGCCTGGATG GCCAGCACAGCTCAAAGACAGAAAGATCCAAATCCTACGATGAAGGACTGGATGATTATAGAGAAGATGC AAAACTATCCTTTAAGCACGTGTCTAGCCTGAAGGGAATCAAGATTACAGACAGCCAGAAGTCATCAGAA GACTCTGGATCGAGAAAGGGTTCTTCCTCCGAGGTCTTCAGTGATGCAGCCCGAGAAGGCTGGCTCCAGT TCCGACCACTGGTCACCGATAAGGGCAAGAGAGTTGGTGGCAGTATTCGGCCATGGAAACAGATGTATGT TGTCCTTCGGGGCCACTCACTTTACTTGTACAAAGACAGAAGAGAGCAGACAACGCCATCAGAAGAAGAG CAGCCCATCAGTGTCAACGCTTGCCTGATAGACATCTCGTACAGTGAGACCAAGAGGCGGAATGTATTCC GACTTACCACGTCTGACTGTGAATGCTTGTTCCAGGCTGAAGACAGAGATGATATGTTATCATGGATCAA GACTATTCAGGAAAGTAGCAACCTAAATGAAGAGGACACTGGAGTTACCAACAGAGATCTAATTAGTCGA AGAATCAAAGAGTATAACAGTCTGCTGAGCAAAACAGAACAGTTGCCAAAAACACCTCGCCAGAGTCTTA GCATCAGGCAAACTTTGCTTGGTGCTAAATCAGAGCCAAAGACTCAAAGCCCACATTCTCCAAAGGAGGA GTCCGAGAGGAAGCTTCTCAGTAAAGATGATACCAGTCCCCCTAAAGACAAAGGTACTTGGAGAAGAGGC ATTCCAAGCATCGTGAGGAAGACGTTTGAGAAAAAGCCGGCCGCCACAGGGACCTTTGGAGTTAGGCTGG ATGACTGCCCACCCGCCCACACCAACAGGTACATTCCATTAATCGTTGACATATGTTGCAAATTAGTTGA AGAAAGGGGTCTTGAATATACAGGTATTTACCGAGTTCCCGGCAACAACGCAGCCATTTCAAGCATGCAG GAAGAACTCAACAAGGGGATGGCAGACATTGACATACAAGATGATAAATGGCGAGATTTGAATGTGATAA GCAGTTTACTAAAATCCTTCTTCAGAAAGCTCCCTGAGCCTCTCTTTACGAATGACAAATATGCTGACTT CATTGAGGCCAATCGCAAAGAGGACCCACTGGATCGGCTACGAACATTAAAGAGACTAATTCATGACCTG CCTGAGCATCATTTTGAGACGCTCAAGTTTCTCTCGGCTCATCTGAAGACAGTGGCAGAGAACTCAGAAA AGAATAAGATGGAACCCAGAAACCTAGCGATCGTTTTTGGTCCAACTCTAGTGAGAACATCAGAAGACAA CATGACCCACATGGTCACGCACATGCCTGACCAGTATAAGATCGTAGAGACTCTCATCCAGCATCATGAC TGGTTTTTCACAGAAGAAGGTGCCGAAGAGCCTCTTACAGCAGTGCAGGAGGAGAACACAGTAGACTCCC AGCCAGTACCAAACATAGATCATTTGCTCACCAACATTGGAAGGACAGGCGTGCTCCCTGGAGATGTATC AGATTCAGCTACTAGTGACTCCGCAAAATCTAAGGGCTCCTGGGGATCCGGGAAGGATCAGTATAGCAGG GAACTGCTTGTATCCTCCATCTTCGCAGCTGCCAGTCGCAAGAGGAAAAAGCCGAAAGAGAAAGCCCAGC CCAGCAGCTCTGAGGATGAGCTGGACAGTGTGTTCTTTAAGAAGGAGAATACGGAACAGAGTCACAGTGA GATTAAAGAAGAGTCCAAAAGAGAAAGCGAGACATCAGGTAGCAAGCAGAGGGTCGTCGTTGCCAAAGAA AGTAACACTAAGAAAGACTCGGGTACAACGAAGGAGGAGAAGAAGATACCGTGGGAAGAACCATCCCCAC CACACAGCTCCAAACGCAACAGGTCACCAACCCTCAGCTGTCGCCTGGCCATGCTGAAAGAGGGCCCCAG ATCACTTCTGACACAGAAGCCCCACTGTGAAGAGACAGGGTCTGACTCAGGCACTCTGCTTAGCACGTCT TCCCAGGCTTCGCTCTTGAGGTCCTCCACTAAAAAATCCACCAGTCCGGAAACCAAGCACAGTGAGTTTT TGTCCATTGCTGGTACCACCACCTCGGATTATTCTACCACATCATCCACTACCTACTTGACCAGCCTGGA CTCCAGCCGCCTGAGCCCTGAGGTGCAGTCAGTGGCCGAGAGCAAGGGGGATGAGGCTGATGACGAGAGG AGCGAGCTGGTGAGTGAAGGACGGCCAGTGGAGACCGACAGTGAGAGTGAGTTCCCCGTGTTTCCCACCA CCCTGACTTCAGACCGGCTGTTCCGAGGAAAATTCCAGGAGGTTGCCAGGGTGAGCCGCAGGAACTCCGA AGGCAGTGAGGCCAGCTGCACCGAGGGAAGCCTCACACCGAGTTTAGACAGCCGCAGGCAGCAGTTCAGC TCCCATAGACTGATCGAATGCGACACACTGTCCAGGAAGAAGTCTGCTCGCTTCAAGTCAGACAGTGGGA GCCCCGGAGACACCAGGACTGAGAAAGAAACTCCTGCCCTGGCCAAGATGTTTGACGTCATGAAGAAAGG CAAGTCGACAGGGAGCCTCCTCACACCCAGCAGAAGTGAATCCGAAAAGCAGGAGGCCACTTGGAAAACA AAAATAGCAGATCGTTTAAAACTGAGACCCAGGGCACCCGCAGACGACATGTTTGGAGTTGGGAATCAAA AACCTACTGCTGAGACAGCGAAAAGGAAAAACATCAAACGCAGACACACGCTTGGAGGCCACAGAGATGC TACTGAGATCAGCGTCCTGAGTTTCTGGAAAGCTCATGAGCAGAGTGCGGACAAAGAGTCTGAACTTTCG GCTGTGAATCGGTTAAAACCCAAATGCTCAGCCCAGGACCTTTCCATCTCAGACTGGCTCGCCAGGGAGC GCGTCCGCACCAGTGCCTCCGACCTTAGCAGAGGCGAAGGCCTCGAGCCCCAGGCTGAGAGCCCCAGCGT GCTGGGAACACCCATCAGCACCCACTCACCACCGTCTCAGCAGCCCGAAGCAAGGGTTGCTGCTACCAGC ACCTTGGCTTCAACCAGCCAGTCCCCTCTTTTCACACCACCACAGTCACCTGACCAAATAAACAGAGAAA GCTTCCAGAACATGAGCCAAAATGCTAGTTCTACAGCTAACATCCACCCCCACAAACAGTCTGAAAGTCC AGACACTAAAGCAGAGACTCCTCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR212100 protein sequence Red=Cloning site Green=Tags(s) MMATHWTGLPEEDGDKLKACGAASACEVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTL RHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIG KTYSQVIALIQNSDTTLELSVMPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSAT AQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDL LANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHS EGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADY NQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRS WDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVN SDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKP SFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLE LPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPND ARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLT TSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSE DSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEE QPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISR RIKEYNSLLSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRG IPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQ EELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDL PEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHD WFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSR ELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKE SNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTS SQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDER SELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFS SHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKT KIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELS AVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATS TLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001081364
ORF Size	5835 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001081364.3, NP_001074833.3
RefSeq Size	6944 bp
RefSeq ORF	5838 bp
Locus ID	71435
UniProt ID	Q6DFV3
MW	215.9 kDa
Gene Summary	Functions as a GTPase-activating protein (GAP) for RHOA and CDC42. Downstream partner of ARF1 which may control Golgi apparatus structure and function. Also required for CTNNA1 recruitment to adherens junctions (By similarity).[UniProtKB/Swiss-Prot Function]

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