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Aak1 (NM_001040106) Mouse Tagged ORF Clone
CAT＃: MR212423
- TrueORF®
Aak1 (Myc-DDK-tagged) - Mouse AP2 associated kinase 1 (Aak1), transcript variant 1
ORF Plasmid: tGFP
"NM_001040106" in other vectors (4)
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CNY 7,032.00
CNY 8,460.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5530400K14Rik; 9630042K20; AU067724; AU067726; BC028270; C79663; D6Ertd245e; mKIAA1048; R75501 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR212423 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAAGTTTTTCGACTCCAGGCGGGAGCAGGGGAGCTCTGGCCTGGGTTCAGGCTCCAGCGGAGGAG GGGGCAGCAGCTCAGGCCTGGGCAGTGGTTACATCGGAAGGGTCTTCGGCATCGGGAGGCAGCAGGTCAC CGTGGACGAGGTGTTGGCGGAAGGTGGATTCGCTCTTGTCTTTCTGGTGAGAACAAGCAATGGGGTGAAA TGTGCCTTGAAACGTATGTTTGTCAACAATGAACATGACCTCCAGGTGTGCAAGAGGGAGATCCAGATCA TGAGAGACCTATCAGGGCACAAGAACATTGTGGGCTACATTGATTCTAGTATCAACAATGTGAGCAGCGG CGACGTCTGGGAAGTTCTCATTCTCATGGACTTCTGCAGAGGAGGCCAGGTGGTAAACCTGATGAACCAG CGTCTCCAGACAGGCTTCACAGAGAATGAAGTGCTGCAGATATTCTGTGACACCTGTGAAGCTGTGGCCC GCCTGCATCAGTGCAAAACTCCCATTATCCACCGGGACCTGAAGGTTGAAAATATCCTCTTGCATGACCG AGGCCACTATGTCTTGTGTGACTTTGGAAGTGCCACCAACAAATTCCAGAATCCACAGGCCGAGGGAGTC AATGCAGTAGAAGATGAGATTAAGAAATACACAACGCTCTCCTATCGAGCCCCAGAAATGGTCAACTTGT ACAGTGGCAAAATCATCACTACGAAGGCAGATATTTGGGCTCTAGGCTGTTTGTTGTATAAATTATGCTA CTTCACTTTGCCGTTTGGGGAGAGCCAGGTGGCGATTTGTGACGGAAGCTTCACAATTCCTGATAACTCT CGTTATTCTCAAGATATGCACTGCCTTATTAGGTATATGTTGGAACCAGACCCTGACAAAAGGCCGGATA TTTACCAGGTTTCATACTTCTCATTTAAACTACTCAAGAAAGAATGCCCCGTTCCAAATGTACAGAACTC TCCCATTCCTGCAAAACTTCCTGAACCAGTGAAAGCCAGTGAAGCAGCTGTGAAAAAGACCCAGCCAAAG GCCAGACTGACCGATCCCATTCCCACCACAGAGACTTCAATCGCACCCCGCCAGAGGCCTAAAGCTGGGC AGACTCAGCCAAACCCAGGCATCCTTCCCATCCAGCCAGCCCTGACTCCTCGGAAGAGGGCCACTGTTCA GCCCCTACCTCAGGCTGCAGGACCCAGCAATCAGCCTGGACTTCTACCCAGTGTTTCCCAACCTAAAGCC CAAGCCACACCCAGTCAACCTCTTCAGTCATCTCAACCCAAGCAACCACAAGCTCCACCCACCCCACAGC AGACACCTGCTACCCAGACACAAGGTTTGCCTACCCAGGCCCAGGCCACTCCCCAGCACCAGCAGCAACA CCTCCTCAAGCAACAGCAGCAACAGCAGCAACAGCCTCAGCAGCCTACAGCACCACCACAGCCTGCAGGC ACCTTTTACCAGCAGCAGCAGCAACAGCAGCAGCAGCAGGCTCAGACTCAGCAGTTTCAGGCAGTACATC CAGCAGCCCAGCAACCAGTCACTGCTCAGTTCCCTGTGGGGTCCCAGGGAGGTGCTCAGCAACAGCTGAT GCAGAACTTCTACCACCAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCTGATGGCTCAGCAGGCTGCC CTGCAGCAGAAGACTGCTGTGGTAGTACCACAGTCTCAGGCACAGCCCGCCACAGCCCCACAGGCAGCCG CTGCCCAGGAGCCTGGGCAGATTCAAGCCCCAGTGAGACAACAGCCAAAAGTTCAGACCACTCCACCTCC AACCATCCAGGGACAGAAAGTTGGATCTCTCACTCCTCCATCATCACCCAAAACCCAACGTGCTGGGCAC AGACGGATTCTCAGTGATGTAACCCACAGTGCAGTCTTTGGGGTTCCTGCCAGCAAATCAACCCAGCTGC TCCAGGCAGCTGCAGCTGAGGCCAGTCTCAATAAATCTAAGTCTGCAACCACCACTCCCTCAGGCTCTCC ACGGACTTCACAGCAGAATGTCTCCAATGCTTCAGAAGGCTCCACATGGAATCCTTTTGATGACGACAAC TTCTCCAAACTCACAGCTGAAGAGCTGCTAAACAAGGACTTCGCCAAGCTTGGGGAAGGAAAGCTGCCTG AGAAGCTTGGTGGCTCTGCAGAGAGTGTGATCCCAGGCTTCCAGCCAACACAAGGAGATGCCTTCACCAC TCCTTCTTTTTCTGCTGGAACTGCTGAAAAAAGGAAGGGGGGCCAGGCTGTGGACTCTGGCATCCCACTT CTAAGTGTGTCTGATCCCTTCATTCCTCTTCAAGTACCTGATGCTCCAGAAAAACTAATCGAGGGACTCA AATCTCCTGACACTTCTCTTCTGCTCCCTGACCTCTTGCCTATGACAGATCCTTTTGGCAGCACTTCTGA TGCTGTTATTGACAAAGCTGATGTTGCTGTTGAGAGTCTCATACCTGGACTGGAGCCACCTGTTGCTCAG CGCCTCCCATCTCAAACGGAATCTGTGACCTCGAACCGCACAGACTCTCTCACGGGGGAAGACTCTCTGC TTGACTGCTCTCTGCTTTCTAACCCTACTGCTGGCCTTCTGGAGGAGTTTGCCCCCATAGCACTCTCTGC TCCCACTCATAAAGCTGCAGAAGATAGCAATCTCATCTCAGGTTTTGGTGTTGCTGAGGGCTCCGAAAAG GTGGCAGAAGATGAGTTTGACCCTATTCCTGTACTGATTACCAAAAACACACAAGGTGGGCACTCTAGGA ACAGCAGTGGGAGCTCTGAGTCCAGTCTTCCCAACCTAGCCAGGTCTTTGCTGCTGGTGGATCAGCTCAT AGACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR212423 protein sequence
Red=Cloning site Green=Tags(s) MKKFFDSRREQGSSGLGSGSSGGGGSSSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFALVFLVRTSNGVK CALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQ RLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQAEGV NAVEDEIKKYTTLSYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPFGESQVAICDGSFTIPDNS RYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPVPNVQNSPIPAKLPEPVKASEAAVKKTQPK ARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPLPQAAGPSNQPGLLPSVSQPKA QATPSQPLQSSQPKQPQAPPTPQQTPATQTQGLPTQAQATPQHQQQHLLKQQQQQQQQPQQPTAPPQPAG TFYQQQQQQQQQQAQTQQFQAVHPAAQQPVTAQFPVGSQGGAQQQLMQNFYHQQQQQQQQQQQLMAQQAA LQQKTAVVVPQSQAQPATAPQAAAAQEPGQIQAPVRQQPKVQTTPPPTIQGQKVGSLTPPSSPKTQRAGH RRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRTSQQNVSNASEGSTWNPFDDDN FSKLTAEELLNKDFAKLGEGKLPEKLGGSAESVIPGFQPTQGDAFTTPSFSAGTAEKRKGGQAVDSGIPL LSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIDKADVAVESLIPGLEPPVAQ RLPSQTESVTSNRTDSLTGEDSLLDCSLLSNPTAGLLEEFAPIALSAPTHKAAEDSNLISGFGVAEGSEK VAEDEFDPIPVLITKNTQGGHSRNSSGSSESSLPNLARSLLLVDQLIDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001040106 |
| ORF Size | 2880 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001040106.1, NM_001040106.2, NP_001035195.1 |
| RefSeq Size | 19363 bp |
| RefSeq ORF | 2880 bp |
| Locus ID | 269774 |
| UniProt ID | Q3UHJ0 |
| MW | 103.3 kDa |
| Gene Summary | Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222855 | Aak1 (untagged) - Mouse AP2 associated kinase 1 (Aak1), transcript variant 1, (10ug) |
CNY 12,260.00 |
|
| MG212423 | Aak1 (tGFP-tagged) - Mouse AP2 associated kinase 1 (Aak1) transcript variant 1, (10ug) |
CNY 9,310.00 |
|
| MR212423L3 | Lenti ORF clone of Aak1 (Myc-DDK-tagged) - Mouse AP2 associated kinase 1 (Aak1), transcript variant 1 |
CNY 10,360.00 |
|
| MR212423L4 | Lenti ORF clone of Aak1 (mGFP-tagged) - Mouse AP2 associated kinase 1 (Aak1), transcript variant 1 |
CNY 10,360.00 |
