Dnajc13 (NM_001163026) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	D030002L11Rik; Gm1124; mKIAA0678; RME-8; Rme8
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR212885 representing NM_001163026 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACATAATTAGGGAAAATAAGGATCTGGCATGTTTCTACACAACCAAGCACTCATGGAGGGGAAAGT ATAAACGAGTCTTTTCAGTTGGAACCCATGCAGTTACTACCTATAATCCCAATACCTTAGAAGTAACAAA TCAGTGGCCTTATGGAGACATTTGTAGCATCAGCCCTGTTGGGAAAGGACAAGGAACAGAGTTCAACCTC ACATTTCGTAAAGGCAGTGGGAAGAAGTCAGAAACGTTGAAGTTTTCTACAGAGCACAGAACAGAGCTGC TTACGGAGGCTTTGAGATTTAGAACTGACTTTGCAGAGGGAAAAATCACAGGAAGGAGATATAACTGCTA CAAGCATCACTGGAGCGATGCAAAGAAACCTGTGGTTTTGGAAGTAACTCCTGGAGGCTTTGACCAAATA AATCCTGTAACCAACAGAGTGCTGTGTTCCTATGACTACAGGAATATTGAAGGCTTCGTAGATCTCTCTG ATTATCAGGGAGGATTTTGTATACTCTATGGAGGATTTAGTAGATTGCATTTATTTTCGTCAGAGCAAAG AGAAGAGATCATTAAAAGTGCAATAGAACACGCTGGCAACTACATAGGTATCTCCCTGCGGATCAGGAAA GAGCCACTGGAATTTGAGCAATATTTGAATCTTCGTTTTGGGAAATACAGCACTGATGAATCTATCACAT CTTTGGCAGAGTTTGTGGTCCAAAAAATATCTCCTCGACATTCGGAACCTGTGAAAAGAGTTCTGGCGGT CACAGAAACATGCTTAGTGGAGCGAGATCCAGCAACCTACAACATTGCAACATTGAAGCCCCTGGGAGAA GTATTTGCATTGGTTTGTGACTCAGAAAACCCACAACTTTTTACCATTGAGTTTATAAAAGGGCAAGTTC GGAAATACTCTTCAACAGAAAGAGATTCCTTGTTAGCAAGCTTGCTGGATGGAGTAAGAGCCTCTGGTAA CAGAGATGTTTGTGTTAAGATGGCACCGACCCACAAAGGCCAGCGATGGGGGTTACTCAGCATGCCTATA GACGAGGAAGTCGAGAGCCTTCACCTCAGATTCCTGGCTGCACCTCCTAATGGCAACTTTGCAGATGCTG TATTCAGGTTCAACGCTAACATTTCCTACAGTGGAGTGCTGCATGCAGTAACACAGGATGGTCTCTTCTC AGAAAACAAGGAAAAGCTAATCAATAATGCAATAACAGCACTGTTGTCTCAAGAGGGGGATGTTGTAGCC TCAAATGCAGAACTTGAGAGCCAGTTCCAAGCTGTGAGGAGGCTTGTAGCCTCCAAAGCTGGTTTCCTGG CCTTCACTCAGCTTCCAAAGTTTCGGGAGCGCCTAGGAATGAAGGTAGTGAAAGCACTCAAGAGAAGCAA CAACGGAGTCATCCATGCGGCTGTGGATATGCTCTGTGCCCTCATGTGTCCTATGCATGATGACTATGAC CTAAGACAAGAGCAGCTAAACAAAGCTTCTCTTCTCTCTTCAAAGAAGTTTCTGGAAAACTTATTGGAGA AATTTAATTCCCATGTGGATCATGGGACCGGTGCCCTAGTAATTAGTTCACTCTTGGACTTCCTTACCTT TGCCCTCTGTGCTCCATATAGTGAGACAACTGAAGGGCAGCAGTTTGATATGCTCTTAGAGATGGTAGCC TCCAATGGAAGAACCCTTTTTAAACTCTTTCAGCATCCTTCCATGGCGATTGTGAAGGGAGCTGGATTGG TTATGAAGGCAATAATAGAGGAAGGTGACAGAGAGATTGCTACTAAGATGCAGGAGCTCGCCCTAAGTGA GGGTGCCTTACCTCGACACTTACACACTGCGATGTTTACAATCAGTTCAGATCAGCGCATGCTTACGAAT AGACAGCTAAGTAGACATCTAGTGGGACTCTGGACCGCTGATAACACAACTGCCACAAACTTGTTGAAAC GCATATTGCCGCCAGGCTTGTTGGCATACTTGGACAGTTCAGATCCAGTTCCGGAGAAGGATGCAGACCG CATGCACGTCAGAGACAATGTGAAAATAGCAATGGATCAATATGGAAAATTTAATAAAGTTCCAGAGTGG CAAAGACTAGCTGGAAAAGCTGCCAAAGAAGTTGAGAAGTTTGCCAAAGAAAAGGTTGACCTTGTGTTGA TGCACTGGAGGGATAGGATGGGCATCGCTCAGAAAGAGAATATAAATCAAAAGCCAGTAGTTCTTCGAAA GAGAAGACAAAGAATAAAAATAGAAGCAAACTGGGATCTCTTCTATTACAGGTTTAGTCAAGACCACGCT AGATCGAACCTCATTTGGAATTTTAAAACACGAGAAGAACTGAAAGATGCCCTTGAGTCTGAGATGCGGA CATTTAACATTGATAGGGAGCTGGGCAGCGCCAGTGTCATCTCCTGGAACCACCATGAGTTTGAGGTTAA ATATGAGTGCCTGGCAGAGGAAATCAAAATAGGAGACTATTACTTGAGATTGTTATTGGAGGAGGATGAA AATGAAGAAAGTGGATCAATAAAAAGATCGTATGAGTTTTTCAATGAGCTCTACCATCGCTTCCTGCTCA CTCCAAAAGTTAACATGAAGTGTTTGTGTTTACAAGCCCTTGCTATCGTTTACGGCAGATGCCATGAAGA AATAGGGCCCTTCACAGATACGAGATACATCATTGGGATGTTAGAAAGGTGCACTGATAAACTCGAGCGG GATAGGCTGATCCTCTTCCTCAACAAGCTGATCCTTAATAAGAAAAACGTTAAGGATCTCATGGACTCAA ACGGCATTAGAATTCTTGTGGACTTGCTAACTCTTGCACATCTCCATGTGAGCCGAGCGACAGTCCCCTT GCAGAGCAATGTGATTGAAGCATCTCCAGATATGAAGAGAGAGAGCGAGAAGGAGTGGTATTTTGGCAAC GCAGACAAAGAAAGGAGTGGGCCGTATGGATTTCATGAGATGCAAGAATTATGGGCCAAAGGAATGTTGA ATGCAAAAACCAGATGTTGGGCTCAAGGCATGGATGGATGGCGACCGCTCCAGGCGATACCTCAGCTTAA GTGGTGCCTGTTAGCCAGTGGGCAGGCTGTTCTGAATGAAACTGACCTTGCTACCTTAATACTGAACATG TTGATCACAATGTGTGGATATTTTCCAAGCAGGGACCAGGATAATGCCATCATTCGTCCTCTGCCCAGAG TGAAAAGACTGCTGTCAGATAGCACCTGCCTTCCCCATGTCATCCAGTTACTGCTGACCTTTGACCCTAT CCTTGTTGAGAAAGTTGCCATCTTACTACATCACATTATGCAAGATAACCCACAGTTACCTCGCCTCTAT CTCAGCGGAGTGTTTTTCTTCATCATGATGTATACAGGTTCCAACGTGCTTCCTGTTGCTCGGTTTTTAA AATATACACACAGCAAACAGGCTTTCAAGTCTGAGGAGACTAAAGGACAAGATATTTTTCAGAGAAGTAT ACTTGGACATATCTTACCTGAAGCAATGGTTTGCTACCTAGAAAATTATGAACCTGAGAAGTTTTCTGAG ATTTTCCTTGGTGAATTTGACACTCCAGAAGCAATCTGGAGCAGTGAGATGCGGCGTCTCATGATAGAGA AGATTGCTGCCCATCTTGCTGACTTCACCCCTCGCCTGCAGAGCAATACAAGAGCACTTTATCAATACTG TCCCATCCCGGTTATTAATTATCCACAGCTAGAAAATGAACTATTCTGTAATATTTATTACCTCAAACAA CTTTGTGATACACTCCGGTTTCCAGATTGGCCAATTAAAGACCCAGTTAAGCTTCTGAAAGATTCCCTTG ACGCTTGGAAGAAGGAAGTAGAAAAGAAGCCACCTATGATGTCCATAGACGATGCTTATGAAGTGCTTAA TCTGCCTATAGGCCAGGGGCTGCATGATGAAAGCAAGATCAGAAAAGCTTACTTTAGACTGGCACAGAAA TACCACCCGGATAAGAACCCGGAAGGAAGGGACATGTTTGAAAAAGTAAATAAAGCATATGAATTTCTAT GTACCAAGTCAACAAAAATAGTGGATGGGCCAGATCCAGAGAATATAATTTTAATTCTGAAAACACAGAG CATCCTTTTCAACCGCCATAAAGAAGAGTTACAGCCTTATAAATATGCAGGATATCCCATGCTAATTAGG ACCATAACAATGGAGACTTCAGATGACCTCCTCTTCTCAAAAGAATCACCACTGTTGCCTGCGGCTGCAG AGCTGGCTTTCCACACAGTCAACTGCTCAGCCCTCAACGCTGAAGAGCTCAGGAGAGAGAATGGCTTAGA GGTATTACAGGAGGCATTTAGTCGCTGTGTGGCTGTCCTGAATCGCTCCAGTAAGCCAAGTGACATGTCA GTGCAGGTGTGTGGACACATAAGCAGATGCTACAGTGTGGCTGCCCAGTTTGAAGAGTGCCGGGAGAAGA TCACAGAAATGCCTGGCATCATCAAGGACCTCTGCCGGGTGCTGTATTTTGGCAAGTGTATCCCACGGGT GGCCGCCCTCGCAGTAGAATGTGTCAGCTCTTTCGCTGTGGATTTCTGGCTACAGACACATCTATTTCAG GCTGGAATTTTGTGGTATCTGCTTGTTTATCTGTTTAACTATGACTATACATTGGAAGAGAGTGGCATTC AGAAAAATGAAGAAACAAACCAGCAGGAAGTAGCCAACAGTCTTGCCAAACTCAGTGTCCATGCTCTGAG TCGCCTTGGTGGGTATTTGTCTGAAGACCAAGCAACCCCAGAAAATCCAACTGTAAGGAAGAGTTTAGCT GGCATGCTGACACCCTACATTGCTCGAAAGCTAGCTGTAGCTAGTGCTACTGAGACCCTGAAGATGCTTA ACAGCAACACAGAGAGCCCGTATTTGATGTGGAACAACTCTACAAGGGCAGAACTGCTTGAGTTTCTTGA GTCACAACAAGAAAATATGATCAAAAAGGGTGATTGTGACAAAACTTATGGGGCAGAATTTGTCTACAGT GAACACGCCAAAGAACTTATCGTGGGTGAGATTTTTGTTAGAGTCTATAATGAAGTCCCAACTTTCCAGC TAGAGGTTCCTAAAGAGTTTGCTGCAAGTCTGTTGGATTACATAGGCTCTCAGGCCCAGTACCTCCACAC GTTCATGGCCATCACTCATGCTGCAAAAGTGGAGTCGGAGCAACATGGGGACCGCTTACCAAGAGTGGAA ATGGCTTTGGAGGCTCTGAGAAATGTGATCAAATACAACCCAGGTTCTGAAAGTGAATGCATCGGGCACT TTAAGTTAATATTTTCTCTTCTCCGAGTCCATGGAGCTGGTCAAGTGCAGCAGCTGGCGTTAGAGGTTGT GAATATAGTGACATCCAACCAAGACTGTGTCAATAACATTGCAGAATCCATGGTTTTGTCCAACTTATTG GCTCTTCTACACTCATTGCCATCGAGCCGTCAGCTTGTCCTGGAGACTCTTTATGCTTTGGCATCAAATA CAAAGATAATCAAAGAAGCCATGGCAAAAGGAGCTTTGATTTATTTATTGGATATGTTCTGCAATTCAAC ACACCCTCAGGTTCGGTCCCAGACAGCTGAACTTTTTGCCAAAATGACAGCAGACAAACTAATAGGTCCA AAGGTGCGGATTACATTGATGAAATTTCTGCCCAGCGTGTTCATGGATGCCATGAGAGACAACCCTGAAG CTGCTGTGCATATATTTGAGGGAACTCACGAAAATCCAGAGCTGATCTGGAACGACAGCTCCAGAGATAA GGTTTCCACAACTGTGAGAGAGATGATGCTGGAGCACTTTAAAAATCAACGGGACAACCCTGATGTGAAC TGGAAGTTGCCTGAAGACTTTGCTGTGGTGTTCGGAGAAGCAGAGGGGGAGCTTGCTGTTGGAGGCGTTT TCTTGAGGATCTTTATTGCACAGCCAGCTTGGGTGCTGAGGAAACCCAGAGAATTTCTTATTGCACTGCT AGAAAAATTAACTGAGCTCCTGGAGAAGAACAATCCACATGGAGAAACTCTGGAAACACTGACCATGGCG ACGGTCTGTCTCTTCAGTGCACAGCCTCAGTTGGCAGATCAAGTCCCGCCATTGGGCCATCTTCCCAAAG TTATCCAGGCGATGAATCACAGAAACAATGCCATTCCTAAGAGTGCAATTCGGGTTATTCACGTCCTGTC TGACAATGAGCTGTGTGTCCGAGCCATGGCGTCCTTGGAGACCATTGGCCCACTGATGAATGGAATGCGA AAGCGAGCAGATACTGTGGGCCTGGCCTGTGAAGCCATCAACCGCATGTTCCAGAAGGAGCAGAGTGAGC TGGTAGCGCAGGCATTGAAAGCAGAGTTGGTCCCATACCTCTTAAAGCTGCTTGAAGGCGTAGGCCTTGA AAACCTCGACAGTCCTGCTGCCACCAAGGCCCAGATTGTCAAAGCTCTTAAGGCAATGACTCGAAGTCTG CAGTATGGAGAACAGGTGAGTGAAATCCTCTCTCGGTCTTCAGTCTGGAGTGCCTTCAAAGACCAGAAAC ATGACTTGTTCATTTCTGACTCCCAAACAGCAGGATACCTCACAGGACCTGGAGTTGCTGGCTACCTTAC AGCAGGTACATCCTCATCAGCCATGTCTAATCTGCCGCCTCCCGTAGACCATGAGGCAGGAGACCTTGGC TATCAGACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR212885 representing NM_001163026 Red=Cloning site Green=Tags(s) MNIIRENKDLACFYTTKHSWRGKYKRVFSVGTHAVTTYNPNTLEVTNQWPYGDICSISPVGKGQGTEFNL TFRKGSGKKSETLKFSTEHRTELLTEALRFRTDFAEGKITGRRYNCYKHHWSDAKKPVVLEVTPGGFDQI NPVTNRVLCSYDYRNIEGFVDLSDYQGGFCILYGGFSRLHLFSSEQREEIIKSAIEHAGNYIGISLRIRK EPLEFEQYLNLRFGKYSTDESITSLAEFVVQKISPRHSEPVKRVLAVTETCLVERDPATYNIATLKPLGE VFALVCDSENPQLFTIEFIKGQVRKYSSTERDSLLASLLDGVRASGNRDVCVKMAPTHKGQRWGLLSMPI DEEVESLHLRFLAAPPNGNFADAVFRFNANISYSGVLHAVTQDGLFSENKEKLINNAITALLSQEGDVVA SNAELESQFQAVRRLVASKAGFLAFTQLPKFRERLGMKVVKALKRSNNGVIHAAVDMLCALMCPMHDDYD LRQEQLNKASLLSSKKFLENLLEKFNSHVDHGTGALVISSLLDFLTFALCAPYSETTEGQQFDMLLEMVA SNGRTLFKLFQHPSMAIVKGAGLVMKAIIEEGDREIATKMQELALSEGALPRHLHTAMFTISSDQRMLTN RQLSRHLVGLWTADNTTATNLLKRILPPGLLAYLDSSDPVPEKDADRMHVRDNVKIAMDQYGKFNKVPEW QRLAGKAAKEVEKFAKEKVDLVLMHWRDRMGIAQKENINQKPVVLRKRRQRIKIEANWDLFYYRFSQDHA RSNLIWNFKTREELKDALESEMRTFNIDRELGSASVISWNHHEFEVKYECLAEEIKIGDYYLRLLLEEDE NEESGSIKRSYEFFNELYHRFLLTPKVNMKCLCLQALAIVYGRCHEEIGPFTDTRYIIGMLERCTDKLER DRLILFLNKLILNKKNVKDLMDSNGIRILVDLLTLAHLHVSRATVPLQSNVIEASPDMKRESEKEWYFGN ADKERSGPYGFHEMQELWAKGMLNAKTRCWAQGMDGWRPLQAIPQLKWCLLASGQAVLNETDLATLILNM LITMCGYFPSRDQDNAIIRPLPRVKRLLSDSTCLPHVIQLLLTFDPILVEKVAILLHHIMQDNPQLPRLY LSGVFFFIMMYTGSNVLPVARFLKYTHSKQAFKSEETKGQDIFQRSILGHILPEAMVCYLENYEPEKFSE IFLGEFDTPEAIWSSEMRRLMIEKIAAHLADFTPRLQSNTRALYQYCPIPVINYPQLENELFCNIYYLKQ LCDTLRFPDWPIKDPVKLLKDSLDAWKKEVEKKPPMMSIDDAYEVLNLPIGQGLHDESKIRKAYFRLAQK YHPDKNPEGRDMFEKVNKAYEFLCTKSTKIVDGPDPENIILILKTQSILFNRHKEELQPYKYAGYPMLIR TITMETSDDLLFSKESPLLPAAAELAFHTVNCSALNAEELRRENGLEVLQEAFSRCVAVLNRSSKPSDMS VQVCGHISRCYSVAAQFEECREKITEMPGIIKDLCRVLYFGKCIPRVAALAVECVSSFAVDFWLQTHLFQ AGILWYLLVYLFNYDYTLEESGIQKNEETNQQEVANSLAKLSVHALSRLGGYLSEDQATPENPTVRKSLA GMLTPYIARKLAVASATETLKMLNSNTESPYLMWNNSTRAELLEFLESQQENMIKKGDCDKTYGAEFVYS EHAKELIVGEIFVRVYNEVPTFQLEVPKEFAASLLDYIGSQAQYLHTFMAITHAAKVESEQHGDRLPRVE MALEALRNVIKYNPGSESECIGHFKLIFSLLRVHGAGQVQQLALEVVNIVTSNQDCVNNIAESMVLSNLL ALLHSLPSSRQLVLETLYALASNTKIIKEAMAKGALIYLLDMFCNSTHPQVRSQTAELFAKMTADKLIGP KVRITLMKFLPSVFMDAMRDNPEAAVHIFEGTHENPELIWNDSSRDKVSTTVREMMLEHFKNQRDNPDVN WKLPEDFAVVFGEAEGELAVGGVFLRIFIAQPAWVLRKPREFLIALLEKLTELLEKNNPHGETLETLTMA TVCLFSAQPQLADQVPPLGHLPKVIQAMNHRNNAIPKSAIRVIHVLSDNELCVRAMASLETIGPLMNGMR KRADTVGLACEAINRMFQKEQSELVAQALKAELVPYLLKLLEGVGLENLDSPAATKAQIVKALKAMTRSL QYGEQVSEILSRSSVWSAFKDQKHDLFISDSQTAGYLTGPGVAGYLTAGTSSSAMSNLPPPVDHEAGDLG YQT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001163026
ORF Size	6729 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001163026.1, NP_001156498.1
RefSeq Size	7697 bp
RefSeq ORF	6732 bp
Locus ID	235567
MW	254.4 kDa

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