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Rap1gds1 (NM_001040690) Mouse Tagged ORF Clone
CAT＃: MR213162
- TrueORF®
Rap1gds1 (Myc-DDK-tagged) - Mouse RAP1, GTP-GDP dissociation stimulator 1 (Rap1gds1), transcript variant 1
ORF Plasmid: tGFP
"NM_001040690" in other vectors (3)
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CNY 6,080.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BC011279; GDS1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR213162 representing NM_001040690
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATAACCTCAGTGACACTTTAAAGAAGCTGAAGATAACAGCTGCTGACAGGACAGAGGGCAGTCTGG AAGGATGCTTGGATTGTCTGCTTCAAGCCTTGGCTCAGAATAATGCAGAAACAAGTGAAAAAATACAAGG AAGTGGAATTCTTCAGTTGTTTGCAAATCTGTTGACGCCACAGGCCTCCTGCACAGCCAAAGTAGCCGAC ATCATAGCAGAAGTAGCCAAAAATGAATTTATGCGAATCCCATGTGTAGATGCTGGATTGATTTCACCAC TGGTACAGCTGTTAAATAGCAAAGACCAGGAAGTGCTGCTTCAAACGGGCAGGGCATTAGGAAACATATG CTACGATAGCCATGAGGGCAGAAGTGCAGTTGACCAAGCAGGTGGTGCACAGATTGTAATTGACCATTTA AGGTCGCTGTGCGGTAGGACAGATCCCGCCAGTGAGAAGCTCATGACTGTCTTTTGTGGCATGCTGATGA ACTATAGCAATGAGAATGATTCCCTTCAGGCCCAGCTTATTAGCATGGGTGTTATCCCGACCTTAGTGAA GCTACTGGGCATTCACTGCCACAATGCAGCACTCACCGAGATGTGTCTCGTTGCGTTCGGGAATTTAGCA GAACTTGAGTCAAGTAAAGAACAGTTTGCTAGCACAAACATTGCTGAAGAGCTGGTAAAACTATTCAAGA AACAAATAGAACACGATAAAAGAGAGATGATTTTTGAAGTTCTAGCGCCATTGGCAGAGAATGATGCTAT TAAGCTGCAGCTGGTTGAAGCAGGTTTGGTAGAGTGTCTCCTAGAGATCGTCCAACAGAAGGTGGATAGC AACAAAGAGGACGACGTTGCCGAGCTTAAAACTGCTTCTGACCTCATGGTTTTATTACTGCTTGGAGATG AGTCCATGCAGAAACTTTTTGAAGGAGGAAAAGGCAGTGTGTTCCAAAGGGTGCTCTCCTGGATCCCATC GAATAACCACCAGCTGCAGCTGGCTGGGGCATTAGCCATTGCAAATTTTGCCAGGAACGATGGAAATTGT ATTCATATGGTAGACAATGGGATTGTAGAAAAACTCATGGATTTGCTGGACAGACACGTAGAAGACGGGA ACGTGACGGTGCAGCACGCCGCACTAAGTGCCCTCAGGAATCTAGCCATCCCAGTTGTAAATAAAGCAAA GATGTTATCAGCTGGTGTCACGGAGACGGTTTTGAAATTCCTTAAATCTGAAATGCCCCCAGTTCAGTTC AAACTCCTGGGAACGCTAAGAATGTTAATAGATGCACAAGCAGAAGCTGCTGAGCAACTGGGAAAGAATG CTAAACTGGTAGAGCGTTTGGTTGAATGGTGTGAAGCCAAAGATCATGCCGGCGTGATGGGCGAATCAAA CAGACTGCTCTCTGCCCTCATCCGACACAGTAAATCAAAAGATGTAATTAAAACCATTGTACAGAGTGGT GGCATCAAGCACCTGGTTACCATGGCAACTAGTGAACATGTAATCATGCAGAATGAAGCTCTTGTCGCCC TGGCACTGATAGCAGCCTTGGAACTGGGTCCCGCTGAGAAGGATCTCGCAAGTGCTCAGCTTGTTCAGAT CTTACACCGACTGCTAGCAGATGAGAGAAGTGCCCCGGAGATCAAGTACAATTCCATGGTCCTGATCTGC GCTCTCATGGGATCCGAATCTCTATACAAGGAAGTACAGGATTTGGCCTTTCTAGATGTTGTCTCCAAAC TTCGAAGTCATGAGAACAAAAGTGTTGCCCAACAGGCCTCGCTTACAGAGCAGAGACTTACAGTGGAAAG C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR213162 representing NM_001040690
Red=Cloning site Green=Tags(s) MDNLSDTLKKLKITAADRTEGSLEGCLDCLLQALAQNNAETSEKIQGSGILQLFANLLTPQASCTAKVAD IIAEVAKNEFMRIPCVDAGLISPLVQLLNSKDQEVLLQTGRALGNICYDSHEGRSAVDQAGGAQIVIDHL RSLCGRTDPASEKLMTVFCGMLMNYSNENDSLQAQLISMGVIPTLVKLLGIHCHNAALTEMCLVAFGNLA ELESSKEQFASTNIAEELVKLFKKQIEHDKREMIFEVLAPLAENDAIKLQLVEAGLVECLLEIVQQKVDS NKEDDVAELKTASDLMVLLLLGDESMQKLFEGGKGSVFQRVLSWIPSNNHQLQLAGALAIANFARNDGNC IHMVDNGIVEKLMDLLDRHVEDGNVTVQHAALSALRNLAIPVVNKAKMLSAGVTETVLKFLKSEMPPVQF KLLGTLRMLIDAQAEAAEQLGKNAKLVERLVEWCEAKDHAGVMGESNRLLSALIRHSKSKDVIKTIVQSG GIKHLVTMATSEHVIMQNEALVALALIAALELGPAEKDLASAQLVQILHRLLADERSAPEIKYNSMVLIC ALMGSESLYKEVQDLAFLDVVSKLRSHENKSVAQQASLTEQRLTVES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001040690 |
| ORF Size | 1821 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001040690.2 |
| RefSeq Size | 3663 bp |
| RefSeq ORF | 1824 bp |
| Locus ID | 229877 |
| UniProt ID | E9Q6Q4 |
| MW | 66.5 kDa |
| Gene Summary | Stimulates GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins) including Rap1a/Rap1b, RhoA, RhoB and KRas, by stimulating the dissociation of GDP from and the subsequent binding of GTP to each small G protein. Able to promote the Ca(2+) release from the endoplasmic reticulum via both inositol trisphosphate (Ins3P) and ryanodine sensitive receptors leading to a enhanced mitochondrial Ca(2+) uptake.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG213162 | Rap1gds1 (tGFP-tagged) - Mouse RAP1 GTP-GDP dissociation stimulator 1 (Rap1gds1) transcript variant 1, (10ug) |
CNY 6,650.00 |
|
| MR213162L3 | Lenti ORF clone of Rap1gds1 (Myc-DDK-tagged) - Mouse RAP1, GTP-GDP dissociation stimulator 1 (Rap1gds1), transcript variant 1 |
CNY 7,980.00 |
|
| MR213162L4 | Lenti ORF clone of Rap1gds1 (mGFP-tagged) - Mouse RAP1, GTP-GDP dissociation stimulator 1 (Rap1gds1), transcript variant 1 |
CNY 7,980.00 |
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