Abca14 (NM_026458) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1700110B15Rik; 4930539G24Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR214657 representing NM_026458 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTCACTACAGATGAAGCAGTTTTCAGTCCTCCTGTGGAAAAACTTCCTTCTAAAGAGCCGGAATG TGGTTGGCTTAGTGGTGGAGATCATCTTGATATTTCTGTTGTTTGTATGGACTTTGACTGTACGCAGAAT TTCAAAGAAGACATCTATAAGTGATGTGATTTTCAATCCTATTCTCTTGACATTGCCTAAATTCCTTAAT GAAAACTTTGACTATGAATTGGCTTATGTGCCTTCTGAGAGTAATGCAGCAAGAAATATTACTGAGATGG TGAAAAAGGATTTAAATTTCAACTTTAAAGTTCAAGGCTTTCCTTCGGAAGAATCTTTTGAGAGATACAT TAAGTATGAGAACAAGGATGCGCATGTGCTGGCTGCTATTGTGTTTGATCACAAGTTCAAAACCAGCAAT GAACGCTTGCCACTTCAGGTAAAATATAGTTTGCGCTTCGGTCGTATCTATGATCCTGAAAATTTGTTTC AGCCGTCTAAGTATCAGAAAGAAATAGAGTGGAATACATCAACTCTCTTCCCATCCGTGCCTTCTCTAGG ACCCCGGAACTTCTTGGAAAACGATGGGGGAAACCCTGGATACATCAGAGAAGGATTTCTTATCGTGCAG CATTCAGTAGACAAGGCCATCATGATGTATCACAGTGGCAGAGCAGCTGAAGACATATTTGCCAACACCA CAATTTATGCGGAGAGATTCCCACACCCGGCCTTTATTCATGACAGCTTCTTGTGGACTTTTATTGCTAT GTTTCCTTGGACAATTTTATTTACATTTACTCAAATGGCACTTGTCATAGTTGGGACCATTATGCTGGAA AAGGAAAAGAGATTAAAGGAATACCAGCTCATGGTGGGACTCAGTAATGCTATGCTCTGGGTTTCCTACT TCATCACCTTCTTATTGATGTATTTCATCATCATCTGTTTGCTGTGTGGGATTTTATTCCTCAAGATTAC ACACGAGAGAGTCTTCCAGCATAGTGACCCGCTCTTCATCGCCTTCTATTTCATGTGTTTTGCCGTCTCC TCAGTGCTGCTCGGCTTCCTGATTAGCACACTCTTTAATAAAGCCTCTCTGGCCACTTCCATTGCAGGGT TCCTCCACTTCCTCACCTTCTTTCCATATTTGATACTATATCATAAGTATGACCAGATAAGCCTCAGTGG GAAGCTGGCTCTCTGTCTCATTACCAACACGGCGCTAGCATTTGGGACTGACTTGATATGTAAGCTGGAA ATGAAGGGGCACGGCGCTCAGTGGAACAACTTTGCTACAAAAGTAAACGCCGATGATGACTTAACCTTGG CCCACATAATAGGAATGTTTTTATTTAGTGCTTTTCTGTATGGCCTTGTGGCCTGGTACTTAGACGCTGT CTTTCCAGGGACGTATGGTGTGCCCAAGCCCTGGAACTTTTTCCTGCAGAAAGCCTACTGGTTTGGAGAG CCAGCCTTAAGCAGGGAAGAGAGCCAAGTCTCTGATTTACTTTCCAGTGATTTTATGGAGCCTGAACCTG TTGGCTTAGTGGCTGGCATCCGGATTCAGCATTTGTACAAGGAATTCATTTTAAAAAACAGTACTCTGAT GGCAGTAAATGACTTGTCTTTGAACTTGTATGAGGGGCAGATAACTGTTCTTCTCGGACATAACGGGGCA GGAAAAACCACCACCTTGTCCATCCTCACAGGTTTGTATCTTCCTACCAGAGGAAAGGTCTATATTAGTG GATATGACATCTCATCTGACATGGTTCAAATTAGAAAAAGCTTGGGCTTGTGTCCCCAGGATGACCTGTT GTTCCCGATGCTGACAGTGTCAGAACACCTTCATTTCTACTGCGTGATCAAAGGAATACCTTTGCAGAAC CAATCAAGAGAAACTAACAGAATGCTCACTTCTTTTGGCCTGCTTCAGCAAAGTAATACCATGTCAAAGG ATCTGAGCGGAGGAATGAAGCGCAAGCTCTCAATCATTATAGCTCTGATAGGGGACACTAAGGTGGTGAT CCTTGATGAGCCAACATCTGGCATGGATCCAGTCTCCAGGAGGGCCACCTGGGACCTACTTCAGCATTAT AAAAAGGACCGTACCATCTTACTGACCACACACCATATGGATGAGGCCGATGTGCTTGGTGACCGGATCG CCATCCTGGTCATGGGGATCCTGAAGTGCTGTGGCTCTTCGCTTTTCCTGAAGAAACTATATGGTGTGGG GTATCACCTAGTCATCGTGAAGACTCCTGACAGCAATGATGAAAAAATCTTCCAGCTGATTAAGAATTAC ATACCGACTGCCAAGATGGAGACCAATGTTGCAGCTGAGCTGTCCTTTATCTTGCCCAAGGAGCACACAC ACAGGTTTGCAGAGCTTTTCACAGACCTAGAAGAAAAACAAGAAGAGCTAGGAATCTCTGGCTTTGGTGT CTCCATGACTACCATGGATGAGGTTTTCTTCAAGGTCAGTAATCTGGAAGATTTGAAATTGAACACTGAG ATTGCTCCATCAGCTTCCATAGTGAGCCAAAGCTCAAATGAAGACAACCAGAACATGAATGTGCCCAGAA ATTTTGAGCGACCAGGTTACTCAAGAAGACACTTGGATTCTTCATTCAATGCCGGGTGGTCTCTTTACAT CCAGCAGTTCAGAGCCATGTTCATCAAAAGGGTAATGTTCTCTTGGCGCTACTGGAAACTGCTGCTGTTG CAGTTGCTGGCACTTCTGGGTCTCATGTATCTTCTGATAAAAGGAATAAGTTTCTCAGTTCCTAAAGAGC CTGCCAGGGTGATGGACTTGGAACAGTATGGCGAGACCATTGTTCCTTTCTCAGTTTCTGGGGACCCCAA TTTGACCCAAATTCTCACCAAAAATCTTGAGACCATGTTGAAGGCTAAAAAGCAAAAAATTCATGAAGTA CAAGGTGATATGAAGAACTACTTGCTGACAAGTAAAGACTGCATTTACTCGTGCATCGTTGCCTTTTCCC TTGATGTTACCACAGAAGAAAAGACATTTATCTTTTGGTTTAACAATGAGGCATACCATGCAGCACCGTT ATCTCTGTCGATATTAGACAACATTATTTATAAGTATCTCTCTGGCCCTGATGCCACCATTACAGTCTCC AACAACCCTCAGCCTCAACGTGTCACCAAAGACAAATCTAATGAAAGAAGCATATCAGGAATCCAGATAG TTTTTAACTTGCTCTTTGGGATGAGTATCTTCACCAGTGGCTTTTGCCTCATGACAGTGACTGAGAGAGT TAGCAAAGCAAAGCACATACAGTTTGTGAGTGGTGTCTATACCTTGAATTTTTGGCTCTCCGCTTTGCTG TGGGACCTCATCATCCACTTCGTGGCCTGTGTCTTGCTGCTGGTAGTGTTCCTATACACTGATGTGGATA TATTGTTGGAGAAATACCACTTCCTGGACACAATGTTCATCCTCATGTTGTTTGGCTGGTCTATTATCCC CTTCATCTACCTACTAAGCTTCTGGTACAACAACAGCACCAATGCCTACATCAAGATCTTTGTCTTCAAC CACTGTTTAGGGTTTATCAGTATAATTGTGGATGCTGTGGTACAAATCATACCAGATATTAAAACCAGTA CTAAGAATCTCATACTTAACTCCATGCTGCTGTTACCCATTTATAACTTTGGGATGAGTATCTACAAATA CTATAACATCCACGAGATAAGGAAATTATGTTCTTCTTTGGGATATATTAATACCATTCCAAGTTGCAAA AGTCAAATTATTGACATTCCTATATACAGTATGAATCAAAAAGCAATCGGAAGGCATGTGACTGCCATGG CTGCCACAGGACTGATATACTTTATCCTCATTATCCTTCTGGAGACTACTTCATGGAATTTAAAGATCTT CATATATCGATATGTTCTCTTTGGCATCTACAGGATATTTTATAAGGCTAGAATGTCTGAGGAGTTATCT GGATACTCAGAAGAGGAAGATGTACAAAATGAAAGAGAGACTATCCTGAACCATCCTTGGCGCTCACTAA ATTCTACTGTGCTCATTAAAGAGCTCATAAAGATATATTTTAAAATTCCACCTACTCTGGCTGTGAGAAA TATCTCTGTCGCCATTCAAAAGGAGGAGTGCTTTGGACTGCTGGGATTAAATGGAGCTGGGAAAACCACT ACCTTTAAAATTCTGACTGGAGAAGAGATAGCTACCTCTGGGGATGTGTTCATTGAAGGCTACAGCATCA CCAGAAATATTTTAAAGGTAAGGTCAAAAGTTGGCTACTGTCCGCAGTTTGATGCCTTGCTGGATTACAT GACCTCTCGGGAGATATTGACAATGTATGCCAGGGTGTGGGGGATTCCAGAGAACAGTATTAGAGCCTAT GTGGACAACTTGTTGAAAATGCTGTATCTGAAACCACAGGCTGATAAGTTTATTTACACTTTGAGTGGAG GAAACAAACGTAGGCTGAGCACTGCCATTGCCATAATGGGAAACTCTACAGTTGTCTTCCTAGATGAGCC ATCCACTGGCATGGACCCACTAGCCCGGCGCATGCTCTGGAACGCAGTGATAAAGACAAGGGAGAGTGGC AAGGTCATCATCATAACCTCTCACAGTATGGAGGAGTGTGAAGCTCTCTGTACCAGGCTAGCCATCATGG TGCAGGGGAAGTTTGTGTGCCTGGGAAGCCCTCAGCATCTCAAGAACAAATTTGGCAATATTTACACCAT GACCATCAAGTTCAAAACTGACACCGATGACAACACGGTACAAGATTTAAAAGATTTTATTGCCGAAGTT TTCCCAGGTAGCGACTTAAAGCAAGAGAATCAAGGGATCCTTAATTACTACATTCCCAGCAAGAACAACA GCTGGGGAAAGGTGTTTGGCATTCTGGAGAAAGCGAAGGAGGACTACAATTTGGAAGATTATTCCATCAG TCAGATCACGCTGGAGCAAGTCTTCCTGACCTTTGCCAATCCAGATAACCCACGGAGCGGCTATGAGAAA GCAGTGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR214657 representing NM_026458 Red=Cloning site Green=Tags(s) MDSLQMKQFSVLLWKNFLLKSRNVVGLVVEIILIFLLFVWTLTVRRISKKTSISDVIFNPILLTLPKFLN ENFDYELAYVPSESNAARNITEMVKKDLNFNFKVQGFPSEESFERYIKYENKDAHVLAAIVFDHKFKTSN ERLPLQVKYSLRFGRIYDPENLFQPSKYQKEIEWNTSTLFPSVPSLGPRNFLENDGGNPGYIREGFLIVQ HSVDKAIMMYHSGRAAEDIFANTTIYAERFPHPAFIHDSFLWTFIAMFPWTILFTFTQMALVIVGTIMLE KEKRLKEYQLMVGLSNAMLWVSYFITFLLMYFIIICLLCGILFLKITHERVFQHSDPLFIAFYFMCFAVS SVLLGFLISTLFNKASLATSIAGFLHFLTFFPYLILYHKYDQISLSGKLALCLITNTALAFGTDLICKLE MKGHGAQWNNFATKVNADDDLTLAHIIGMFLFSAFLYGLVAWYLDAVFPGTYGVPKPWNFFLQKAYWFGE PALSREESQVSDLLSSDFMEPEPVGLVAGIRIQHLYKEFILKNSTLMAVNDLSLNLYEGQITVLLGHNGA GKTTTLSILTGLYLPTRGKVYISGYDISSDMVQIRKSLGLCPQDDLLFPMLTVSEHLHFYCVIKGIPLQN QSRETNRMLTSFGLLQQSNTMSKDLSGGMKRKLSIIIALIGDTKVVILDEPTSGMDPVSRRATWDLLQHY KKDRTILLTTHHMDEADVLGDRIAILVMGILKCCGSSLFLKKLYGVGYHLVIVKTPDSNDEKIFQLIKNY IPTAKMETNVAAELSFILPKEHTHRFAELFTDLEEKQEELGISGFGVSMTTMDEVFFKVSNLEDLKLNTE IAPSASIVSQSSNEDNQNMNVPRNFERPGYSRRHLDSSFNAGWSLYIQQFRAMFIKRVMFSWRYWKLLLL QLLALLGLMYLLIKGISFSVPKEPARVMDLEQYGETIVPFSVSGDPNLTQILTKNLETMLKAKKQKIHEV QGDMKNYLLTSKDCIYSCIVAFSLDVTTEEKTFIFWFNNEAYHAAPLSLSILDNIIYKYLSGPDATITVS NNPQPQRVTKDKSNERSISGIQIVFNLLFGMSIFTSGFCLMTVTERVSKAKHIQFVSGVYTLNFWLSALL WDLIIHFVACVLLLVVFLYTDVDILLEKYHFLDTMFILMLFGWSIIPFIYLLSFWYNNSTNAYIKIFVFN HCLGFISIIVDAVVQIIPDIKTSTKNLILNSMLLLPIYNFGMSIYKYYNIHEIRKLCSSLGYINTIPSCK SQIIDIPIYSMNQKAIGRHVTAMAATGLIYFILIILLETTSWNLKIFIYRYVLFGIYRIFYKARMSEELS GYSEEEDVQNERETILNHPWRSLNSTVLIKELIKIYFKIPPTLAVRNISVAIQKEECFGLLGLNGAGKTT TFKILTGEEIATSGDVFIEGYSITRNILKVRSKVGYCPQFDALLDYMTSREILTMYARVWGIPENSIRAY VDNLLKMLYLKPQADKFIYTLSGGNKRRLSTAIAIMGNSTVVFLDEPSTGMDPLARRMLWNAVIKTRESG KVIIITSHSMEECEALCTRLAIMVQGKFVCLGSPQHLKNKFGNIYTMTIKFKTDTDDNTVQDLKDFIAEV FPGSDLKQENQGILNYYIPSKNNSWGKVFGILEKAKEDYNLEDYSISQITLEQVFLTFANPDNPRSGYEK AVP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_026458
ORF Size	5049 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_026458.4, NP_080734.3
RefSeq Size	5384 bp
RefSeq ORF	5052 bp
Locus ID	67928
MW	192.3 kDa

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