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Gpr3 (NM_008154) Mouse Tagged ORF Clone
CAT＃: MR214992
- TrueORF®
Gpr3 (Myc-DDK-tagged) - Mouse G-protein coupled receptor 3 (Gpr3)
ORF Plasmid: tGFP
"NM_008154" in other vectors (4)
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CNY 3,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Gpcr3; Gpcr20; Gpcr21 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR214992 representing NM_008154
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTGGGGAGCAGGAAGCTCTATGGCCTGGTTCTCAGCTGGCTCAGGCAGTGTGAACGTGAGCAGCG TGGACCCAGTAGAGGAACCCACAGGCCCAGCTACACTGCTGCCCTCTCCCAGGGCCTGGGATGTGGTGCT GTGCATCTCAGGCACCCTGGTGTCCTGCGAGAACGCGCTGGTGGTGGCCATCATTGTGGGCACTCCTGCC TTCCGCGCCCCCATGTTCCTGCTGGTGGGTAGCTTGGCCGTAGCAGACCTGCTGGCAGGCCTGGGCCTGG TCCTGCACTTTGCGGCTGACTTCTGCATTGGCTCACCAGAGATGAGCTTGATGCTGGTCGGCGTGCTAGC AATGGCCTTCACTGCCAGCATCGGCAGCCTGCTGGCCATTACCGTTGACCGCTACCTTTCCCTGTACAAT GCTCTCACTTACTACTCAGAGACAACGGTAACTCGGACTTATGTGATGCTGGCCTTGGTGTGGGTGGGTG CCCTGGGCCTGGGGCTGGTTCCCGTGCTGGCCTGGAACTGCCGGGATGGCCTGACCACGTGTGGTGTGGT CTATCCACTCTCCAAGAACCATCTGGTGGTTCTGGCCATCGCCTTCTTCATGGTGTTTGGCATCATGTTG CAGCTCTATGCCCAGATCTGCCGCATCGTCTGCCGCCATGCCCAGCAGATCGCCCTCCAACGACACCTGC TGCCTGCCTCTCACTACGTGGCCACCCGCAAGGGCATCGCCACATTGGCTGTGGTGCTTGGCGCCTTTGC TGCCTGTTGGTTGCCCTTCACTGTCTACTGCCTCCTGGGAGATGCCGACTCTCCCCGTCTCTACACCTAT CTTACCCTGCTCCCAGCCACCTACAACTCCATGATCAACCCTGTCATCTACGCCTTTCGCAACCAAGATG TGCAGAAGGTGCTGTGGGCCATCTGCTGCTGCTGTTCCACTTCCAAGATTCCATTCCGGTCCCGGTCCCC TAGTGATGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR214992 representing NM_008154
Red=Cloning site Green=Tags(s) MMWGAGSSMAWFSAGSGSVNVSSVDPVEEPTGPATLLPSPRAWDVVLCISGTLVSCENALVVAIIVGTPA FRAPMFLLVGSLAVADLLAGLGLVLHFAADFCIGSPEMSLMLVGVLAMAFTASIGSLLAITVDRYLSLYN ALTYYSETTVTRTYVMLALVWVGALGLGLVPVLAWNCRDGLTTCGVVYPLSKNHLVVLAIAFFMVFGIML QLYAQICRIVCRHAQQIALQRHLLPASHYVATRKGIATLAVVLGAFAACWLPFTVYCLLGDADSPRLYTY LTLLPATYNSMINPVIYAFRNQDVQKVLWAICCCCSTSKIPFRSRSPSDV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008154 |
| ORF Size | 990 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008154.3, NP_032180.1 |
| RefSeq Size | 2159 bp |
| RefSeq ORF | 993 bp |
| Locus ID | 14748 |
| UniProt ID | P35413 |
| MW | 35.9 kDa |
| Gene Summary | Orphan receptor with constitutive G(s) signaling activity that activate cyclic AMP. Has a potential role in modulating a number of brain functions, including behavioral responses to stress, amyloid-beta peptide generation in neurons (By similarity) and neurite outgrowth (By similarity). Maintains also meiotic arrest in oocytes.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208582 | Gpr3 (untagged) - Mouse G-protein coupled receptor 3 (Gpr3), (10ug) |
CNY 3,600.00 |
|
| MG214992 | Gpr3 (tGFP-tagged) - Mouse G-protein coupled receptor 3 (Gpr3), (10ug) |
CNY 4,370.00 |
|
| MR214992L3 | Lenti ORF clone of Gpr3 (Myc-DDK-tagged) - Mouse G-protein coupled receptor 3 (Gpr3) |
CNY 5,890.00 |
|
| MR214992L4 | Lenti ORF clone of Gpr3 (mGFP-tagged) - Mouse G-protein coupled receptor 3 (Gpr3) |
CNY 5,890.00 |
