H2bw2 (NM_027067) Mouse Tagged ORF Clone
CAT#: MR215388
- TrueORF®
H2bfm (Myc-DDK-tagged) - Mouse RIKEN cDNA 1700014N06 gene (1700014N06Rik)
ORF Plasmid: tGFP
"NM_027067" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1700014N06Rik; H2bfm; H2bfw; H2BL; H2bl2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR215388 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCTACCACGGCTATGGACGTTCTGGAGGAGCTATCTTCGGATAGTTCCGAAAAACAGGTACAAC CAAGAAAGCCTGAGAAAGCTAAAAGGGAAAAGGACAAACCAAAGAAAGGGGGGCCGGAGAAGAAGGCCAA GAAAGAGAAACAGGAGAAAGCAAAGCCGGAGAAAAAGCCGAAGAAGAAGCCAGAGAAAGAGAAGCCGGAG GGAGAGAAGCTGGAGAAGAAACCCAAGAAAGACAAGCGGGAGAAAGCGAAGCCGAAGAAGAAGCCCGAGC AAGAGAACCGTGAGCAAGAGACTCCTGAGCAGGAGAAGCCTGAGGTGCAGCGGCGTCGCTCACTTCACCA AAGCATCAGGGAAGATGAGCGTAGAGCCCGTCTGATCAGACGCCGTAAGAACAGCTTCGCTATCTACTTT CCGAAGGTGCTGAAAAATATCCACGTGGGTCTCTCCCTCTCGCAGCGGTCTGTGAACATCCTGGATTCAT TCGTGAAGGATATGTTTGAAAGAATTGCATCCGAAGCCAGCTTCTTGGCGCGTCAAGCCAGAAACTCTAC TATCAACTCCAGAGAGATCCAGACCGCTATTCGACTTCTGCTTCCTGGCGAGCTCTGCCGGCGTGCAGTG GCTGAAGGAACCATGGCGATGGTCCGGTATATCTCCAACAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR215388 protein sequence
Red=Cloning site Green=Tags(s) MASTTAMDVLEELSSDSSEKQVQPRKPEKAKREKDKPKKGGPEKKAKKEKQEKAKPEKKPKKKPEKEKPE GEKLEKKPKKDKREKAKPKKKPEQENREQETPEQEKPEVQRRRSLHQSIREDERRARLIRRRKNSFAIYF PKVLKNIHVGLSLSQRSVNILDSFVKDMFERIASEASFLARQARNSTINSREIQTAIRLLLPGELCRRAV AEGTMAMVRYISNK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_027067 |
ORF Size | 675 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_027067.2, NP_081343.1 |
RefSeq Size | 1049 bp |
RefSeq ORF | 675 bp |
Locus ID | 69389 |
MW | 26.1 kDa |
Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the H2B histone family. [provided by RefSeq, Nov 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210926 | H2bfm (untagged) - Mouse RIKEN cDNA 1700014N06 gene (1700014N06Rik), (10ug) |
CNY 3,990.00 |
|
MG215388 | H2bfm (tGFP-tagged) - Mouse RIKEN cDNA 1700014N06 gene (1700014N06Rik), (10ug) |
CNY 2,850.00 |
|
MR215388L3 | Lenti ORF clone of H2bfm (Myc-DDK-tagged) - Mouse RIKEN cDNA 1700014N06 gene (1700014N06Rik) |
CNY 4,750.00 |
|
MR215388L4 | Lenti ORF clone of H2bfm (mGFP-tagged) - Mouse RIKEN cDNA 1700014N06 gene (1700014N06Rik) |
CNY 4,750.00 |