Nolc1 (NM_001039352) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	NOPP130; NOPP140; P130
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR215401 representing NM_001039352 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGATACCGGCTTGCGCCGCGTGGTTCCCAGCGACCTTTATCCCCTTGTGCTCAGATTTCTGCGGG ATAGCCAACTCTCGGAGGTGGCCAGTAAATTTGCAAAAGCGACCGGCGCTACACAGCAGGACGCCAATGC CTCGTCCCTCTTGGACATCTATAGCTTCTGGCTCAACAGGTCCACCAAAGCCCCAAAGGTGAAGTTACAG TCAAATGGACCAGTGACCAAGAAGGCTAAGAAAGAGACTTCATCCAGTGACAGCAGTGAGGACAGCAGTG AGGACGAGGACAAAAAAGCCCAGGGACTTCCCACACAGAAGGCTGCCGCACAGGTCAAGCGAGCCAGTGT GCCTCAGCATGCTGGAAAGGCAGCAGCCAAAGCTTCAGAGAGCAGCAGTAGTGAAGAATCCAGTGAGGAA GAGGAAGAGGACAAAAAGAAAAAGCCTGTCCAGCAGAAGGCAGCTAAGCCCCAAGCCAAGGCAGTCAGAC CTCCTGCGAAGAAGGCAGAGAGCTCTGAGTCGGACTCAGACTCGGATTCGGACTCCAGCTCAGAGGAAGA AACACCACAGACCCAGAAGCCAAAGGCAGCTGTGGCAGCAAAAGCTCAGACTAAAGCCGAAGCCAAACCA GGTACACCAGCGAAAGCACAGCCTAAGGTAGCCAATGGCAAAGCAGCCGCCAGCAGCAGCAGCAGCAGCA GCAGCGATGACTCAGAGGAAGAGAAGAAGGCAGCTGCACCTCCCAAGAAGACTGTACCAAAAAAGCAAGT CGTGGCCAAGGCCCCAGTGAAAGTAGCTGCCGCCCCCACCCAGAAGAGCTCCAGCAGTGAGGATTCTTCC AGTGAAGAGGAGGAGGGACAGAGACAACCCATGAAGAAAAAAGCAGGTCCCTACAGTTCAGTTCCACCAC CCTCTGTTCCTTTACCAAAGAAGTCCCCGGGAACCCAGGCTCCAAAGAAAGCTGCTGCGCAGACACAGCC TGCAGACAGCAGTGACGACAGCAGTGACGATTCTGATTCAAGTTCTGAGGAAGAGAAAAAACCTCCAGCT AAGACGGTCGTCTCCAAGACACCCGCCAAAGCAGCTCCAGTGAAGAAGAAAGCAGAAAGCTCTTCAGACA GCTCGGATTCTGACAGTTCTGAGGATGAAGCTCCTGCCAAGCCAGTCAGTACAACCAAGAGTCCCAAGCC AGCTGTCACTCCGAAGCCATCTGCAGCAAAGGCAGTGACAACTCCTAAGCAACCTGCAGGCAGTAACCAG AAACCTCAGAGCAGGAAGGCTGACAGCAGCTCCAGCGAGGAGGAAAGCAGCTCCAGCGAGGAGGAGGAGG CCTCCAAGAAAAGTGCCACAACCCCCAAGGCCAAGGTGACTGCTAAAGCAGCACCCGCCAAACAGGCCCC TCAGGCTGCTGGGGACAGCAGCTCTGACTCAGATAGTTCCAGCAGTGAAGAGGAGGAGAAGACTCCTAAG CCCCCAGCTAAGAAGAAGGCAGCAGGTGGAGCCGTTTCTACACCAGCCCCTGGGAAGAAAGCAGAGGCCA AGAGCAGCAGCAGCAGCAGCAGCAGCAGCTCCGAAGATTCCAGTGAAGAGGAGAAAAAAAAGAAGCCCAA AGCTACTACCCCTAAAATACAGGCAAGCAAGGCCAATGGCACTCCAGCTTCTCTGAATGGAAAAGCAGCC AAGGAAAGTGAGGAGGAAGAGGAGGAGGAAGAAACAGAAGAGAAGAAAAAGGCAGCTGGGACCAAGCCAG GTTCAGGCAAAAAACGGAAGCAGAATGAGACCGCAGATGAAGCAACAACTCCTCAAGCTAAGAAAGTTAA GCTCGAGACCCCCAATACGTTTCCAAAAAGGAAGAAGGGAGAAAGAAGGGCGTCTTCCCCTTTCCGAAGG GTCAGGGAGGAGGAGATTGAGGTGGACTCTCGAGTGGCGGACAATTCCTTTGATGCCAAGCGAGGTGCAG CTGGAGACTGGGGGGAGCGAGCCAATCAGGTTCTGAAGTTCACCAAAGGCAAGTCCTTCCGGCATGAAAA AACGAAGAAGAAGCGAGGCAGCTACCGGGGAGGCTCCATCTCTGTCCAGGTCAATTCCGTCAAATTCGAC AGCGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR215401 representing NM_001039352 Red=Cloning site Green=Tags(s) MADTGLRRVVPSDLYPLVLRFLRDSQLSEVASKFAKATGATQQDANASSLLDIYSFWLNRSTKAPKVKLQ SNGPVTKKAKKETSSSDSSEDSSEDEDKKAQGLPTQKAAAQVKRASVPQHAGKAAAKASESSSSEESSEE EEEDKKKKPVQQKAAKPQAKAVRPPAKKAESSESDSDSDSDSSSEEETPQTQKPKAAVAAKAQTKAEAKP GTPAKAQPKVANGKAAASSSSSSSSDDSEEEKKAAAPPKKTVPKKQVVAKAPVKVAAAPTQKSSSSEDSS SEEEEGQRQPMKKKAGPYSSVPPPSVPLPKKSPGTQAPKKAAAQTQPADSSDDSSDDSDSSSEEEKKPPA KTVVSKTPAKAAPVKKKAESSSDSSDSDSSEDEAPAKPVSTTKSPKPAVTPKPSAAKAVTTPKQPAGSNQ KPQSRKADSSSSEEESSSSEEEEASKKSATTPKAKVTAKAAPAKQAPQAAGDSSSDSDSSSSEEEEKTPK PPAKKKAAGGAVSTPAPGKKAEAKSSSSSSSSSSEDSSEEEKKKKPKATTPKIQASKANGTPASLNGKAA KESEEEEEEEETEEKKKAAGTKPGSGKKRKQNETADEATTPQAKKVKLETPNTFPKRKKGERRASSPFRR VREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSVKFD SE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001039352
ORF Size	2106 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001039352.2, NP_001034441.1
RefSeq Size	3665 bp
RefSeq ORF	2109 bp
Locus ID	70769
UniProt ID	E9Q5C9
MW	73.7 kDa
Gene Summary	Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (By similarity). Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (By similarity). Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus (PubMed:11424213). It has intrinsic GTPase and ATPase activities (By similarity).[UniProtKB/Swiss-Prot Function]

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