Lmo7 (NM_201529) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C78582; FBXO20; Gm914; mKIAA0858
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR215486 representing NM_201529 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGGATGGAGGACGCCGAGGCCGATTGCTCTGTTGCATTCGCAGAGGCTCAGAGATGGGTGGAGG CTGTAACTGAGAAGAATTTTGAAACAACGGATTTTCGAGCCTCTCTAGAAAATGGTGTTCTGTTGTGCGA CTTGATTAATAAGCTCAAACCTGGCGTCGTTAAGAAGATCAATAGACTGTCTACCCCAATAGCAGGTTTG GATAACATAAATGTTTTTTTGAGAGCTTGTGAACAGATTGGATTGAAAGAAGCCCAGCTTTTTCATCCGG GGGATCTACAGGACTTATCAAACCGAGTCACCGTCAAGCAAGAAGAGACTGACAGGAGACTGAAAAATGT TTTGATAACTTTGTACTGGCTGGGAAGAAAAGCACAAAGTAACCCCTACTATAATGGTCCCTATCTCAAT TTGAAAGCTTTTGAGACTCTTTTAGGGCAAGCTTTGACTAAGGCACTTGAAGATTCCAGCTGCCTGAAAA GAAGTGGCCGGGACAGTGGCTACGGGGACATCTGGTGTCCTGACAGAGGAGAATTTCCTGCCCCTCCAGG TAGCCACAGAAGGGAAGATTCCTTTGAGAGCTTGGACTCGCTGGGGTCCAGATCTCTGACCAGCTGTTCC TCAGACTTCACTCTGAGAGTTGGGAAGGAAGGTTGCGAAAGTGACATCGATTGGGAATTTACATTCAAAA TGCAGGATTACAACAAAGATGATATGTCCTACCGAAGGATTTCAGCCATTGAGCCAAAGTCAGCTCTCCC GTTCAATCGTTTCTTGCCGAACAAAAGCAAGCAGCCGTCCTATGTGCCAGCACCTTTGAGGAAGAAGAGG CCAGACAAACACGAGGATAACAGGAGGAGCTGGGCAAGCCCGGTCTACACAGAAACAGACGGAACATTTT CAAGTACCCAGAGGAGAACCTGGGGTCCCAAGATGGAGACCTGGCACACAGTCCAAGAAACTTCCACCTC TTCTTGGTGTGTAGAAGAGGAAGAGGAAAAGCTCACGCGCATGCCCAACATTGTGAAGGATGATCTGTAT GTGCGGAAGCTTAGCCCCGTCATGCCAAGTCCAGGGAGCTCTTTTGATCAGTTCCTTCCCAAATGTTGGA CTCCAGAAGATATGAACTGGAAAAAAATTAAAAGAGAGACTTATAAGCCATGGTATAAGGAATTTCAGGG ATTCAGTCAGTTTTTATTGCTTCAGGCCCTCCAAACCTACTCTGATGACATCTTGTCCTCGGAAACAAGT GTCAAACTCGATCCTACCTCTGGCCCCAGGCTCATAACTCGCAGGAAGAATCGCTCTCCCGCACCAGGCT ATCGAGCCCATGACCTTGAGCTGCCAGCCCTAGATCCCGACTTAGAAAATGATGATTTCTTTGTCAGAAA AACAGGGGCTTTCCATGCAAATCCGTGTGTCCTTCGAGCCTTTGAAGACTTTAGAAGTTTCTCGGAGCCA GACGATGCCGTGGAACGAGATATAATTTTGCAGTGTCGGGAAGGTGAACTCGTACTTCCAGATTTAGAAA AGGATGATATGATTGTCCGCAGGATTCCAGCTCAGAAGAAAGAAGTGCCCCTTTCTGGGGCCCCGGATAG GTACCAGCCAGTCCCTTTCCCTGAACCCTGGACCCTACCTCCAGAAATCCAAGCGAAGTTTCTCTCTGTG CTCGAAAGGACACGCCCACCCAAAGAACAAAGCAGCGGCTGTAGAGTGTTAGTCCCTTCCTATCGACAGA AGAAAGACGACATGTTGGCTCGCAAGATCCAGTCTTGGAAGCTGGGGACTGCTGTACCTCCCATCAGTTT CAAGCCTGGCCCCTGCAGCGAGGCCGACCTGCAGAAGTGGGAGGCCATCTGGGAGGCCAGCAGGCTTAGG CACAGGAAGCGGCTGATGGTTGAGAGACTCTTTCAAAAGATCTATGGTGAGAATGGGAGCAAGTCCATGA GCGATGTCAGCGCAGAGGATGTTCAGAACCTGCGTCAGGTGCGCTACGAGGAGATGCAGAAAATAAAATC CCAGCTGAAAGAACAAGATCAGAAATGGCAGGATGACCTTGCAAAATGGAAAAACCGCCGGAAGAGTTAC ACTTCAGATCTGCAGAAGAAAAAGGAAGAGAGGGAAGAGATTGAGAAGCAGGCACTGGAGAAGTCTGACC GCAGCTCCAAGACATTTAGGGAAATGCTGCAGGACAGGGAATCTCGAGGTTCTACAGTTACGTCTTGGAG GAGGACCTATTCCTCTGATGACATCCTCGATGATGGAGTGCTCCCTCCTACAGTGACTCTGTCAGAAACT AGTTACCAGAGCGAGAGACTAGAAGATGAGGCAAGAGCGCATCCTGCTGAGATGCCCAAAGAAGATTCCA CAACATTTGCAAATAGAGAGGACAGTGTAGTAGCTGAGACTCAGCTTGCTTCTCACAGTCCTGAAGAGCA ACGTCTGGCCCCTTCTCCTTCTGAGGAACCGCGCCAGGCCTCTGGATCTTCTGCAGAACCGCACCAGGCC CCGTTGCTGTCCACAGAACCACGCCGGACCCCTTTGCCTTCCGAGGAACCACACCAGGCCTCTCTATCTT CTACACTACAGCGTCCATCCTCACTCTCTTCTCAGCAGTCATGGAGTACAAGAGCAGAGTCCACTCGGAT TTCAGCTTCTCTCCCTAGAAGTTACCAGAGAACCGATACAGCCAGGTTAACATCTGTGGTCACAGCGAGA CCTTTTGGCACATCTTCAAGGGGAATCTCCTCACTGCCCAGATCCTACACGATGGATGATGGCTGGAAAT ACAACGGAGATGTTGAAGTTGTCAAGAGAGAGCAACTCGATTTGGGCAGAGCCACTGGCCCGAAGCCTGA CTCCAGCCACTTTATTTCGGGCTCAGCCAGTGAGAAGGAGGTCATAGCCACAGAAGATGTGGCAAGCTTG TCCTCTCCCACACTCCCTTCCTCATCTCTCTCCCACGACCGGGCAGTCTCTTCCAAAGCCACGTTCTCAT CAATGTCTGGTTTGGATTCAGTGTCTGACTCTGGAGAAGGAAGAGGCTCACCATTGAGGGAAGTTTCCAG GTCCCTGGATCAGTTCAGCGATATGAGAGTCAGCATAAACCAGACGCCCGGGAACAAACCTGTCTTTGGG TTTACGATAAAATGGGATATTTCTGGGATCTTCGTAGCATCAGTCGAACAAGGTAGCCCAGCAGAATTTT CTCAGCTGCAGGTAGATGATGAAATTCTTGCCATCAATAACACCAAGTTTTCATATAAGGATACAAAAAA GTGGGAGGAAGTTATGGCAAACGCCCAAGAAACTGGAAACTTAGTGATGGACGTCCGGCGTTACGGAAAG TCTGGTTCATCTGAAACAAAGTGGATCGATACAACATCAGGAATTTACAACTCAGACAAGTCTTCAAGTT TATCAGTCACAACTGATTTCTCTGAAAGCCTTCAGAGTCCTTATACTGAATCCAAAGAAGTCAATGGAAT TCACGAGGAAAGTAACCCCTTTGATTCAAAAGCATCAGAATCCATTTCTTTGAAAAATTTAAAAAGGCGA TCACAATTTTTTGAACAAGGAAGCTCGGATTCTGTGGCCCCCGATCTTCCAGTTCCAACCCTCAGTGCCC CGAGTCGCTGGGCTTGGGATCAAGAGGAGGAGAGACGGAGGCAGGAGCGGTGGCAGAAAGAGCAAGATCG CCTATTGCAGGAAAAATACCAGCGTGAGCAGGAGAAGCTGAGGGAGGAGTGGCAGAGAGCCCAACAGGAG GCGGAGAGAGAGAATTCCAAGTACCTGGATGAGGAGCTGATGGTCCTAAATTCCAACAGCATTTCTCTGA CCTCCCGGGAGCCTGTGGCCGCCACCTGGGAAGCCACGTGGAGTGAAGGGTCCAAGTCTCTGGACAGCGA AGGCACCCGAGCGGGAGAGGAGGACAGGGGACAGCTGGAAGACGATGCTGTGTATGAGGACCAAAGTCAG AAGCTGCAGGAACTTGAGCAGGAGAAGAAACGGAAGGAGCAAGAGGCTCAGGAAGAGGAGCGCCGCAAGC AGCGGGAGGCCCAGGCCTGGGCAGAGGCAGAAGCTAAGGCTTGGGCGGAGGCCCAGGCTCGGGCAGAGGC AGAAGCTAAGGCTCGGGCGGAGGCCCAGGCCCGGGCAGAGGCTGAGGCTCAAAAACGAGCTGAAGCTCAG AAGCTCCAGGCAGAGAGGGAACGTGAAACGTCCGTCAAAATATACCAGTACCGAAGGCCCATTGATTCCT ACGACCTACCAAAGAGAGAAGACGACACTTCAGGGATGCTTCCAACTGACAGGAGTAAATCAAGATCTAC TACGGAACTAAATGACCCCCTCATAGAGAAAAATGGAAGCAGCAAATATTCAGAGAGAATTGGGGCCACT AGCTTTTCACATAGGAGTTCTAAGAAGGATCAAGCCCCCTCAGAAGCAGAGTTGGAGAGACAGCAAATCC TTCAGGAAATGAGGAAGAGAACATCTCTCCACAATGACAACAGCTGGATCCGACAGCGCAGCGCCAGTGT GAACAAGGAGCCCATCTGCCTGCCTGGGATCATGAGGAGAGGGGAGTCTCTGGACAATCTGGATTCCCAG CGGCCGGGTTCTTGGAGGCAGTCCCCTTGGCACAGCCAGCCTGCCGGGGTCTATGCGTCTTCCTCTGTCC AGGACTTCAGTCGCCCCCCACCTCAGCTGTTGTCTACATCAAACCGTGCCTACATGCGGAACCCATCCTC TGCGGTGCCACCTCCAGCCGGCTCTGTGAAGACCTCCCCAGGGTCTCCCTCTCCACGCAGCCATTCCCCT TCCATGTCACAGTCAGGCTCTCAGCTACGGAATAGGTCAGTGAGTGGGAAGCGTGTGTGCTCCTACTGTA ATAACATTCTGGGCAAAGGAGCCGCCATGATCATCGAGACCCTGGGTCTTTCTTATCATTTACATTGTTT TAAGTGTGTCTCCTGTGGGTGTGACCTCGGAGGCTCTTCCTCAGGAGCTGAAGTCAGAATCCGAAACCAC CAACTCTACTGCAATGACTGCTATCTCCGATTCAAATCTGGACGGCCAACCGCCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR215486 representing NM_201529 Red=Cloning site Green=Tags(s) MEGMEDAEADCSVAFAEAQRWVEAVTEKNFETTDFRASLENGVLLCDLINKLKPGVVKKINRLSTPIAGL DNINVFLRACEQIGLKEAQLFHPGDLQDLSNRVTVKQEETDRRLKNVLITLYWLGRKAQSNPYYNGPYLN LKAFETLLGQALTKALEDSSCLKRSGRDSGYGDIWCPDRGEFPAPPGSHRREDSFESLDSLGSRSLTSCS SDFTLRVGKEGCESDIDWEFTFKMQDYNKDDMSYRRISAIEPKSALPFNRFLPNKSKQPSYVPAPLRKKR PDKHEDNRRSWASPVYTETDGTFSSTQRRTWGPKMETWHTVQETSTSSWCVEEEEEKLTRMPNIVKDDLY VRKLSPVMPSPGSSFDQFLPKCWTPEDMNWKKIKRETYKPWYKEFQGFSQFLLLQALQTYSDDILSSETS VKLDPTSGPRLITRRKNRSPAPGYRAHDLELPALDPDLENDDFFVRKTGAFHANPCVLRAFEDFRSFSEP DDAVERDIILQCREGELVLPDLEKDDMIVRRIPAQKKEVPLSGAPDRYQPVPFPEPWTLPPEIQAKFLSV LERTRPPKEQSSGCRVLVPSYRQKKDDMLARKIQSWKLGTAVPPISFKPGPCSEADLQKWEAIWEASRLR HRKRLMVERLFQKIYGENGSKSMSDVSAEDVQNLRQVRYEEMQKIKSQLKEQDQKWQDDLAKWKNRRKSY TSDLQKKKEEREEIEKQALEKSDRSSKTFREMLQDRESRGSTVTSWRRTYSSDDILDDGVLPPTVTLSET SYQSERLEDEARAHPAEMPKEDSTTFANREDSVVAETQLASHSPEEQRLAPSPSEEPRQASGSSAEPHQA PLLSTEPRRTPLPSEEPHQASLSSTLQRPSSLSSQQSWSTRAESTRISASLPRSYQRTDTARLTSVVTAR PFGTSSRGISSLPRSYTMDDGWKYNGDVEVVKREQLDLGRATGPKPDSSHFISGSASEKEVIATEDVASL SSPTLPSSSLSHDRAVSSKATFSSMSGLDSVSDSGEGRGSPLREVSRSLDQFSDMRVSINQTPGNKPVFG FTIKWDISGIFVASVEQGSPAEFSQLQVDDEILAINNTKFSYKDTKKWEEVMANAQETGNLVMDVRRYGK SGSSETKWIDTTSGIYNSDKSSSLSVTTDFSESLQSPYTESKEVNGIHEESNPFDSKASESISLKNLKRR SQFFEQGSSDSVAPDLPVPTLSAPSRWAWDQEEERRRQERWQKEQDRLLQEKYQREQEKLREEWQRAQQE AERENSKYLDEELMVLNSNSISLTSREPVAATWEATWSEGSKSLDSEGTRAGEEDRGQLEDDAVYEDQSQ KLQELEQEKKRKEQEAQEEERRKQREAQAWAEAEAKAWAEAQARAEAEAKARAEAQARAEAEAQKRAEAQ KLQAERERETSVKIYQYRRPIDSYDLPKREDDTSGMLPTDRSKSRSTTELNDPLIEKNGSSKYSERIGAT SFSHRSSKKDQAPSEAELERQQILQEMRKRTSLHNDNSWIRQRSASVNKEPICLPGIMRRGESLDNLDSQ RPGSWRQSPWHSQPAGVYASSSVQDFSRPPPQLLSTSNRAYMRNPSSAVPPPAGSVKTSPGSPSPRSHSP SMSQSGSQLRNRSVSGKRVCSYCNNILGKGAAMIIETLGLSYHLHCFKCVSCGCDLGGSSSGAEVRIRNH QLYCNDCYLRFKSGRPTAM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_201529
ORF Size	5097 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_201529.2, NP_963287.2
RefSeq Size	6123 bp
RefSeq ORF	5100 bp
Locus ID	380928
MW	192.6 kDa

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