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Pcgf2 (NM_009545) Mouse Tagged ORF Clone
CAT＃: MR215739
- TrueORF®
Pcgf2 (Myc-DDK-tagged) - Mouse polycomb group ring finger 2 (Pcgf2), transcript variant 1
ORF Plasmid: tGFP
"NM_009545" in other vectors (5)
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CNY 2,850.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | mel-18; Mel18; Rnf110; Zfp144 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR215739 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATCGGACCACACGGATTAAAATCACGGAGCTGAACCCTCACCTCATGTGTGCCCTCTGTGGGGGCT ATTTTATTGATGCCACCACCATTGTGGAATGCTTACATTCCTTCTGCAAAACCTGCATCGTACGCTACTT GGAGACCAACAAATACTGCCCCATGTGTGACGTCCAGGTCCATAAAACTCGACCACTGCTGAGCATCAGA TCAGACAAAACCCTCCAGGACATTGTCTACAAGTTGGTGCCTGGGCTTTTTAAAGATGAAATGAAACGGC GACGGGACTTCTATGCAGCATACCCCCTGACAGAGGTCCCCAACGGCTCCAACGAGGACCGAGGCGAGGT CCTGGAACAGGAGAAGGGGGCTCTGGGTGACGACGAGATTGTCAGTCTTTCCATTGAGTTCTACGAAGGC GTCAGGGACCGAGAGGAGAAGAAGAACCTCACGGAGAATGGAGATGGGGACAAGGAGAAGACAGGAGTGC GATTCCTGCGGTGCCCAGCAGCCATGACCGTCATGCACCTGGCAAAGTTCCTCCGCAACAAAATGGATGT GCCCAGCAAGTACAAGGTGGAGATCCTCTATGAAGATGAGCCCCTGAAGGAATACTACACCTTAATGGAC ATTGCGTACATCTACCCCTGGCGAAGGAATGGGCCTCTCCCCCTCAAGTACCGTGTCCAGCCAGCCTGCA AGCGGCTCACCCTGCCCACGGTGCCAACCCCCTCAGAAGGCACCAACACCAGCGGGGCATCAGAGTGTGA GTCAGTCAGCGACAAGGCTCCCAGCCCTGCCACCCTTCCGGCCACCTCCTCCTCCTTGCCCAGCCCTGCG ACCCCATCCCATGGCTCTCCCAGCTCCCACGGCCCCCCAGCTACCCATCCTACTTCCCCCACTCCACCTT CAACTGCCGCTGGGACCACCACAGCTACCAATGGGGGCACTTCGAACTGCCTGCAAACGCCATCCTCCAC CAGCAGGGGGCGCAAGATGACTGTTAATGGCGCTCCTTGCCCCCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR215739 protein sequence
Red=Cloning site Green=Tags(s) MHRTTRIKITELNPHLMCALCGGYFIDATTIVECLHSFCKTCIVRYLETNKYCPMCDVQVHKTRPLLSIR SDKTLQDIVYKLVPGLFKDEMKRRRDFYAAYPLTEVPNGSNEDRGEVLEQEKGALGDDEIVSLSIEFYEG VRDREEKKNLTENGDGDKEKTGVRFLRCPAAMTVMHLAKFLRNKMDVPSKYKVEILYEDEPLKEYYTLMD IAYIYPWRRNGPLPLKYRVQPACKRLTLPTVPTPSEGTNTSGASECESVSDKAPSPATLPATSSSLPSPA TPSHGSPSSHGPPATHPTSPTPPSTAAGTTTATNGGTSNCLQTPSSTSRGRKMTVNGAPCPP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009545 |
| ORF Size | 1029 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009545.2, NP_033571.1 |
| RefSeq Size | 2752 bp |
| RefSeq ORF | 1029 bp |
| Locus ID | 22658 |
| UniProt ID | P23798 |
| MW | 37.7 kDa |
| Gene Summary | Transcriptional repressor (PubMed:8521824). Binds specifically to the DNA sequence 5'-GACTNGACT-3' (PubMed:8521824). Has tumor suppressor activity (PubMed:8521824). May play a role in control of cell proliferation and/or neural cell development (Probable). Regulates proliferation of early T progenitor cells by maintaining expression of HES1(PubMed:15728456). Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells (PubMed:8625838, PubMed:15183898). Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202903 | Pcgf2 (untagged) - Mouse polycomb group ring finger 2 (Pcgf2), transcript variant 1, (10ug) |
CNY 2,000.00 |
|
| MG205143 | Pcgf2 (tGFP-tagged) - Mouse polycomb group ring finger 2 (Pcgf2) |
CNY 3,140.00 |
|
| MG215739 | Pcgf2 (tGFP-tagged) - Mouse polycomb group ring finger 2 (Pcgf2) transcript variant 1, (10ug) |
CNY 3,140.00 |
|
| MR215739L3 | Lenti ORF clone of Pcgf2 (Myc-DDK-tagged) - Mouse polycomb group ring finger 2 (Pcgf2), transcript variant 1 |
CNY 4,750.00 |
|
| MR215739L4 | Lenti ORF clone of Pcgf2 (mGFP-tagged) - Mouse polycomb group ring finger 2 (Pcgf2), transcript variant 1 |
CNY 4,750.00 |
