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Ell2 (NM_138953) Mouse Tagged ORF Clone
CAT＃: MR215768
- TrueORF®
Ell2 (Myc-DDK-tagged) - Mouse elongation factor RNA polymerase II 2 (Ell2)
ORF Plasmid: tGFP
"NM_138953" in other vectors (4)
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CNY 4,760.00
CNY 5,610.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR215768 representing NM_138953
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGGGGGGGCTGCGGGACTGCGGGAGGAGCAGCGGTACGGGCTGGCGTGCGGGCGGCTGGGGC AGGACAACATCACCGTGCTGCATGTGAAGCTCACCGAGACGGCGATCCGGGCGCTGGAGACTTACCAGAG CCACAAGAATTTAATTCCTTTTAGACCTTCAATTCAGTTCCAAGGACTCCAAGGGCTTATGAAAATTCCC AAAAATGATCCCTTCAATGAAGTCCAGAACTTTAACTTCTACTTGTCAAACGTGGGAAGAGATAATCCTC AGGGCAGCTTTGACTGCATCCAGCAAACACTCTCTAGCTCTGGAGCCTCCCAGCTCAATTGCCTGGGATT TATACAAGATAAAATTACAGTGTGTGCAACAAATGATTCCTATCAGATGACGCGAGAAAGAATGACCCAG GCAGAGGAGGAATCCCGTAATCGAAGCACAAAAGTTATCAAACCCGGTGGACCATATGTAGGGAAAAGAG TGCAAATTCGTAAAGCTCCTCAAGCCATCTCGGACACAGTGCCTGAGAGGAAAAGGTCAACGCCCATGAA CCCTGCAAATACAATTCGAAAGATGCATAGTGGCAACAGTGTCTCTCAGAGGCCATACAGGGACAGGGTC ATCCATTTACTGGCACTAAAAGCCTACAAGAAACCTGAGCTGCTTGCTCGACTGCAGAAAGATGGTGTCA ATCAAAAAGACAAGAACTCCTTGGGAGCAATTCTGCAACAGGTGGCCAATCTGAATCCTAAGGACCTCTC CTATACTTTAAAGGATTATGTATTTAAGGAGCTCCAGCGAGACTGGCCAGGTTACAGTGAGACAGACAGA CAGACATTGGATTTGGTGCTCTCTAGAAAACTAAATCCATCTCAGAATGCTAGCACCAGCCGGTCAGAGT CTCCCCTGTGTTCCAGCAAAGACGCTGCCTCATCTCCTCAGAAACGACCTTTGGATTCAGATTTCATTGA TCCTTTAATGAACAAAAAAGCTCGAATATCTCACCTAACAAACAGAGTCCCACCGACATTAAATGGTTAT TTGAATCCCACCAGTGAAAAATCTTGTGCAGGCCTCCTACCACCCCCTGCAGCTGCTGCCATCCCCACCC TCTCGCCGCTGCCTTCAACCCACCTGCCTGTCTCCAATCCTCCTCAGACTGTAAATTCCAACTCCAATTC CCCTAGCACTCCAGAAGGCCTGGGGACTCAAGACCTGCCTGTTGACAGTTTTAGTCAAAATGGTAGCATC TTTGAGGACCAGCAAGAAAAATATACCTCAAGGACTTGTCTGGAAACATTACCCCCCAGCTCAGCTCTGC TAAAGTGTCCAAAGCCCATGGAAGAAGAGCATCCAGTGTCTCACAAAAAGTCCAAAAAGAAGTCTAAAAA ACACAAGGAAAAGGACCAAATAAAGAAACTTGACATTGAGACCATGGAGGAGAAGGAGGAAGACCTTCAG AGAGAAGAAACTGCCAAGCTGAGTAATGCCAGTCCAAATCCCAATGAAGGAGTTAAAGAAGGGTGCACAG CCTCCATGGAGCCTTCTTCAGCACTTGAACTCCCAGATTATTTGATAAAATATATTGCTATTGTCTCTTA TGAGCAACGCCAGAATTACAAGGATGACTTCAATGCTGAGTATGATGAATACAGAGCTTTGCATGCAAGG ATGGAGACTGTAGCCAGGAGATTTATTAAACTGGATGCACAACGAAAACGCCTTTCTCCGGGTTCAAAAG AGTACCAGAATGTTCATGAAGAAGTCTTACAGGAATATCAGAAGATCAAGCAGTCCAGTCCCAATTACCA TGAAGAAAAATACAGATGTGAATATCTTCATAACAAGCTGGCTCACATCAAAAGACTAATAGGTGAATTT GACCAACAGCAAGCAGAGTCATGGCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR215768 representing NM_138953
Red=Cloning site Green=Tags(s) MAAGGAAGLREEQRYGLACGRLGQDNITVLHVKLTETAIRALETYQSHKNLIPFRPSIQFQGLQGLMKIP KNDPFNEVQNFNFYLSNVGRDNPQGSFDCIQQTLSSSGASQLNCLGFIQDKITVCATNDSYQMTRERMTQ AEEESRNRSTKVIKPGGPYVGKRVQIRKAPQAISDTVPERKRSTPMNPANTIRKMHSGNSVSQRPYRDRV IHLLALKAYKKPELLARLQKDGVNQKDKNSLGAILQQVANLNPKDLSYTLKDYVFKELQRDWPGYSETDR QTLDLVLSRKLNPSQNASTSRSESPLCSSKDAASSPQKRPLDSDFIDPLMNKKARISHLTNRVPPTLNGY LNPTSEKSCAGLLPPPAAAAIPTLSPLPSTHLPVSNPPQTVNSNSNSPSTPEGLGTQDLPVDSFSQNGSI FEDQQEKYTSRTCLETLPPSSALLKCPKPMEEEHPVSHKKSKKKSKKHKEKDQIKKLDIETMEEKEEDLQ REETAKLSNASPNPNEGVKEGCTASMEPSSALELPDYLIKYIAIVSYEQRQNYKDDFNAEYDEYRALHAR METVARRFIKLDAQRKRLSPGSKEYQNVHEEVLQEYQKIKQSSPNYHEEKYRCEYLHNKLAHIKRLIGEF DQQQAESWH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138953 |
| ORF Size | 1917 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138953.2, NP_620403.2 |
| RefSeq Size | 3660 bp |
| RefSeq ORF | 1920 bp |
| Locus ID | 192657 |
| UniProt ID | Q3UKU1 |
| MW | 72.6 kDa |
| Gene Summary | Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (By similarity). Plays a role in immunoglobulin secretion in plasma cells: directs efficient alternative mRNA processing, influencing both proximal poly(A) site choice and exon skipping, as well as immunoglobulin heavy chain (IgH) alternative processing. Probably acts by regulating histone modifications accompanying transition from membrane-specific to secretory IgH mRNA expression.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220029 | Ell2 (untagged) - Mouse elongation factor RNA polymerase II 2 (Ell2), (10ug) |
CNY 8,270.00 |
|
| MG215768 | Ell2 (tGFP-tagged) - Mouse elongation factor RNA polymerase II 2 (Ell2), (10ug) |
CNY 6,180.00 |
|
| MR215768L3 | Lenti ORF clone of Ell2 (Myc-DDK-tagged) - Mouse elongation factor RNA polymerase II 2 (Ell2) |
CNY 7,510.00 |
|
| MR215768L4 | Lenti ORF clone of Ell2 (mGFP-tagged) - Mouse elongation factor RNA polymerase II 2 (Ell2) |
CNY 7,510.00 |
