Muc20 (NM_001145874) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BC026367
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR215789 representing NM_001145874 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTCTGTCTGGGGCCTGGCTGTGCCCCTCCTTGTCTTCTGCTGGAAGGTTGGCGTCAGTGTGAGCT CTCCAGGTCTCGACATTAGCAGATCAGTTCCTTTGGTGACAACCAACAACATGGAAGTGTCCACCTTCAC TCAAAGGGACAGGCCCAGCTCAGAGAGAGCTTTCCAGACCACCAACCTGATTCAGTATGTACCTCTAGAC ACGCAAACTCTCAGCACTGAATCTGCTTCTAATGCTTTAAGTGCAACTAGTATCAGTTCAGAGGTCAATT CCAGAGACACTCAGACCACCTCTTTTGTAACAAAGACCAGGAAGACACATACTACCACTCCTGCAGCATC ATCCCTAGAGGCCCAGACCACCTCTCCTAATTTGTCAACCCTGAATATTCAGATTACCTCCCCAATAGCA TCGTCCCTGGATGCTCAGACCATTTTTCCTGTATCATTATCCCTGAGTACCCAGACCACTTCTCCTGCAC CATCATTCCTGGATACCCAAACCACTTCCCCTGAACCATCATCTCTGACCACTTCCCCTGCACCATCATC TCTGATCACTTCTCCTACTCCATCATCTCTAACTACATCCCCTGCACCATCATTCCTGGATACCCAGACC ACTTCCCCTGCACCATCATCTCTGACCACTTCCCCTGCACCATCATCCCTAGATACCCAGACCATCTCAC CTATTGAATTGACCCTAAAGACCCAAACCATTTCCACTGTAACGGAGACCAGAACGGTTTCAATAAGAAT ACCTTCTGACCTCACGGTTATGCACACCATCCCTACAGAGACATTGGCACCAAGCAACAGCCCCAGAACT GGGATGAGCACTGTCCAAACTGGCACAGTGTGGGACTCCATAGAAGCCGTCTTTGACACCCTTTGTACCG ATGACAGCTCTGAAGAGGCAAGGAAGATCACAGTTGACCTCCTGACGTTGGCACACACCTCCACGGAAGT GGAGTACCTGTCTTCAGAGAGCAGCTCTTCCTCTGACAGTTCAGCTGGGGTCCTCAGCAGCTCACGAGTC CTGGGACCCGACAGTGCAACTCCAGCCAAGGGGTTGGTTGCCTTCAACATCACTCATATTAAACTCAGCA ACTGCATCACAGAAATAGAAACAACCATCACCATCTCTGGGGCCCCAGGCGCGAGCCTCAGCCCTACAGA AGCGACGGCAGCTTTGTTCACTTCTGAGATATTAACTTTGCCTCCACCCACTGAGGCAAAACCAATTTTC CCCGAGACCACAAGCTTATCTGGGATCCTGTCAACTGCTGGCACCCCGGCCCTTGCCACCACCCTTGAGG GGACAGTCTCCACTAGTGCAATCACAGAAAGCGAAACAGCAGTGGCCCAGACTCTTACCTCTGTTGGAAC TTCGGTGACAGTTAGAAGGAACCCCCTGGAAAACACTTCAACACTCTCTATTGAGACACAAAGCCACACT GAGGTCTTGGGCACCATCACAGTCCCCATGGTTGCTGGATCAACAATGGGAGAAGCAGCCTCCTTCGTGA GTTTCACGGCTCTCGACAGCAGCAGTCTCTCAGTGGTGGTCACCACCGAGAGCTCTGCTACCTCAGAGAC TTTAACCACAGGCAATACAACCAACAGCTCCTTCCTCACAGAGAGCCACCCTCCTTTTTCTATCTATTCA ACTACAGCTAGCACCAGCAAAAACCCAAACATCACCTTAACCAAGACCACAGCCTCACCAAAGCCCCCGA CGCATCCTACAACATCTGCATCCACTGCTTGGATAAGGAAAACCACAAAACATGATCCAGGTGAAGATGG AGGCTTCCTCCTTGTACGGCTGACCGTGGCCTCCCCTAAGGACCTCACTGAGCACAACGCCAGAGAGAAG CTGATGAACCAGATAGTGACTCACGAGCCATCTGGTCCTGGACATCTGCCCTCGAGATGGGGAAGAAGTG GACGTCCTTCCTGGCTTCTCCACTCCTCTTCTGCCTCTCCTGCCGAGCCTGCAGGAGGAAGGCCTCCATC ACGGCGCCTATCAGCTGGTTGTTCAGATAGTGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR215789 representing NM_001145874 Red=Cloning site Green=Tags(s) MGSVWGLAVPLLVFCWKVGVSVSSPGLDISRSVPLVTTNNMEVSTFTQRDRPSSERAFQTTNLIQYVPLD TQTLSTESASNALSATSISSEVNSRDTQTTSFVTKTRKTHTTTPAASSLEAQTTSPNLSTLNIQITSPIA SSLDAQTIFPVSLSLSTQTTSPAPSFLDTQTTSPEPSSLTTSPAPSSLITSPTPSSLTTSPAPSFLDTQT TSPAPSSLTTSPAPSSLDTQTISPIELTLKTQTISTVTETRTVSIRIPSDLTVMHTIPTETLAPSNSPRT GMSTVQTGTVWDSIEAVFDTLCTDDSSEEARKITVDLLTLAHTSTEVEYLSSESSSSSDSSAGVLSSSRV LGPDSATPAKGLVAFNITHIKLSNCITEIETTITISGAPGASLSPTEATAALFTSEILTLPPPTEAKPIF PETTSLSGILSTAGTPALATTLEGTVSTSAITESETAVAQTLTSVGTSVTVRRNPLENTSTLSIETQSHT EVLGTITVPMVAGSTMGEAASFVSFTALDSSSLSVVVTTESSATSETLTTGNTTNSSFLTESHPPFSIYS TTASTSKNPNITLTKTTASPKPPTHPTTSASTAWIRKTTKHDPGEDGGFLLVRLTVASPKDLTEHNAREK LMNQIVTHEPSGPGHLPSRWGRSGRPSWLLHSSSASPAEPAGGRPPSRRLSAGCSDSG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001145874
ORF Size	2064 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001145874.1, NP_001139346.1
RefSeq Size	3673 bp
RefSeq ORF	2067 bp
Locus ID	224116
UniProt ID	Q8BUE7
MW	72 kDa
Gene Summary	May regulate MET signaling cascade. Seems to decrease hepatocyte growth factor (HGF)-induced transient MAPK activation. Blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway. Inhibits HGF-induced proliferation of MMP1 and MMP9 expression (By similarity).[UniProtKB/Swiss-Prot Function]

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