Arhgap21 (NM_001128084) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	5530401C11Rik; AA416458; ARHGAP10
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR215805 representing NM_001128084 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCCACACATTGGACTGGTCTGCCTGAGGAAGATGGTGACAAACTCAAGGCCTGTGGGGCGGCCT CTGCCTGTGAGGTGTCAAAAAACAAAGATGGAAAAGACCAAGGTGAACCTGTGTCACCGTCTGAAGATGA GCCATTCTCCTGGCCAGGTCCCAAAACAGTCATGCTGAAGAGAACATCTCAAGGGTTTGGTTTCACATTA CGACACTTCATCGTTTATCCCCCAGAGTCTGCAATCCAGTTTTCATATAAGGATGAAGAGAATGGAAACA GAGGAGGAAAGCAAAGGAACCGATTGGAGCCGATGGATACCATATTTGTTAAACAAGTGAAGGAAGGAGG GCCGGCCTTCGAGGCTGGCTTATGCACAGGTGACAGAATTATAAAAGTCAATGGAGAAAGTGTCATAGGA AAGACCTATTCCCAAGTAATTGCTCTCATTCAGAACAGTGACACCACCCTGGAGCTCAGTGTAATGCCCA AGGATGAAGACATTCTCCAAGTGCTACAATTTACAAAGGATGTTACAGCACTGGCATATTCTCAAGATGC ATACCTGAAAGGCAATGAAGCTTACAGCGGCAATGCCCGTAATATACCTGAACCTCCACCAGTTTGCTAT CCCTGGTTGCCATCGACTCCTTCAGCTACTGCACAGCCGGTGGAAACATGCCCTCCTGATTCATTACCGA ACAAACAGCAAACCAGTGCACCAGTCCTCACACAACCTGGCAGGGCCTATAGAATGGAAATACAAGTGCC TCCGTCACCAACAGATGTTGCAAAGTCAAACACAGCAGTGTGTGTTTGCAATGAGAGTGTGAGGACCGTC ATTGTGCCTTCTGAGAAGGTGGTAGACTTGCTAGCCAATAGAAACAACCCTTCAGGTCCTTCACATAGAA CTGAAGAGGTAAGGTATGGTGTAAATGAGCAGGCCTCTACAAAAGCAGCATCGAGAACAACGTCACCGGC ATCAGTTCCCACTGCCCATCTGATTCATCAGACCACAGGCTCCAGATCCTTAGAACCTTCTGGAATTTTA CTTAAATCTGGAAATTACAGTGGACATTCAGAAGGAATCTCAAGCAGTAGATCACAAGCCGTGGATTCCC CTCCTGTGTCTGTCAATCACTACTCTGCAAACTCCCATCAGCACATAGACTGGAAAAATTATAAGACTTA CAAAGAATACATTGATAATAGGAGATTGCACATAGGTTGTCGGACAATCCAGGAAAGACTGGACAGCTTA CGAGCTGCATCTCAGAGCGCAGCAGACTATAACCAGGTGGTACCCACCCGCACTACTCTGCAGGTACGAC GTCGAAGCACCTCTCATGACCGAGTGCCCCAGTCTGTTCAGATCCGGCAGCGCAGTGTATCTCAGGAAAG ACTGGAAGATTCTGTGCTGATGAAGTATTGCCCAAGGAGCGCGTCTCAAGGAGCGCTGACCTCACCGCCT GTTAGCTTCAATAATCATAGAACTCGTTCGTGGGATTACATAGAGGGACAGACGGAAGCCACAGCCACTG TCAATTCTGAAAGTCAGATACCTGACTCAAATGGGGAACGAAAACAGACTTACAAGTGGAGTGGCTTCAC TGAGCAGGATGATAGACGCGGTATTCACGAAAGACCCAGGCAGCAAGAAATGCACAAGCCTTTCCGAGGC TCAAATTTGACTGTGGCTCCCGTTGTTAATTCTGATAACAGGCGCCTGGTTGGGAGAGGGGTAGGACCTG TCTCACAGTTTAAAAAAATCCCGCCGGACCTAAGACCACCACATTCCAACAGAAATTTTCCAACCACGAC TGGGGTGTCACTTCAACGGGGTATTGCACAAGACAGGTCCCCACTCGTGAAAGTCCGAAGTAATTCTCTG AAAGTGCCACCTCCACCTGTGTCAAAACCGTCCTTCAGCCAGCACTCACTGGCTTCCATGAAAGACCAAA GGCCTGTCAATCATCTGCATCAACACAGTGTGCTGAGTCAGCAGACGCAGTTCAGATCCGAAAGCACCTT CGAGCACCAGCTGGAGACTGAGGTATCCTCGTGTCTACCAGGAACTTCTGCCAAGACCAGTCCACAGCTC AGTGAGAATTTGGGAACATCAGACTTAGAACTACCTGCCATCCCAAGGAATGGAGATATAAACTTACAGG AGGCTGAAATTCAGCAGCCAGATGTGCTAGATAATAAAGAATCTGTCATTCTAAGAGAGAAACCTCAGTC TGGCCGCCAGACGCCACAGCCTTTAAGACATCAGTCTTACATCCTGGCAGTGAATGACCAGGAGACCGGG TCAGACACCACCTGCTGGCTGCCAAACGATGCACGCAGAGAGGTCCATATAAAAAGAATGGAGGAGAGGA AAGCTTCAAGCACCAGCCCTCCTGGTGATTCCCTGGCATCCATCCCTTTTATAGATGAGCCAACCAGTCC TAGCATCGACCACGAGATTGCACATATTCCTGCATCTGCTGTCATCTCCGCCTCTACTGCCCACGTCCCC TCCATTGCAACTGTTCCCCCCAGCCTCACGACCTCTGCTCCTTTGATTCGACGTCAGCTCTCCCACGACC AAGAATCTGTTGGACCTCCCAGCCTGGATGGCCAGCACAGCTCAAAGACAGAAAGATCCAAATCCTACGA TGAAGGACTGGATGATTATAGAGAAGATGCAAAACTATCCTTTAAGCACGTGTCTAGCCTGAAGGGAATC AAGATTACAGACAGCCAGAAGTCATCAGAAGACTCTGGATCGAGAAAGGGTTCTTCCTCCGAGGTCTTCA GTGATGCAGCCCGAGAAGGCTGGCTCCAGTTCCGACCACTGGTCACCGATAAGGGCAAGAGAGTTGGTGG CAGTATTCGGCCATGGAAACAGATGTATGTTGTCCTTCGGGGCCACTCACTTTACTTGTACAAAGACAGA AGAGAGCAGACAACGCCATCAGAAGAAGAGCAGCCCATCAGTGTCAACGCTTGCCTGATAGACATCTCGT ACAGTGAGACCAAGAGGCGGAATGTATTCCGACTTACCACGTCTGACTGTGAATGCTTGTTCCAGGCTGA AGACAGAGATGATATGTTATCATGGATCAAGACTATTCAGGAAAGTAGCAACCTAAATGAAGAGGACACT GGAGTTACCAACAGAGATCTAATTAGTCGAAGAATCAAAGAGTATAACAGTCTGCTGAGCAAAACAGAAC AGTTGCCAAAAACACCTCGCCAGAGTCTTAGCATCAGGCAAACTTTGCTTGGTGCTAAATCAGAGCCAAA GACTCAAAGCCCACATTCTCCAAAGGAGGAGTCCGAGAGGAAGCTTCTCAGTAAAGATGATACCAGTCCC CCTAAAGACAAAGGTACTTGGAGAAGAGGCATTCCAAGCATCGTGAGGAAGACGTTTGAGAAAAAGCCGG CCGCCACAGGGACCTTTGGAGTTAGGCTGGATGACTGCCCACCCGCCCACACCAACAGGTACATTCCATT AATCGTTGACATATGTTGCAAATTAGTTGAAGAAAGGGGTCTTGAATATACAGGTATTTACCGAGTTCCC GGCAACAACGCAGCCATTTCAAGCATGCAGGAAGAACTCAACAAGGGGATGGCAGACATTGACATACAAG ATGATAAATGGCGAGATTTGAATGTGATAAGCAGTTTACTAAAATCCTTCTTCAGAAAGCTCCCTGAGCC TCTCTTTACGAATGACAAATATGCTGACTTCATTGAGGCCAATCGCAAAGAGGACCCACTGGATCGGCTA CGAACATTAAAGAGACTAATTCATGACCTGCCTGAGCATCATTTTGAGACGCTCAAGTTTCTCTCGGCTC ATCTGAAGACAGTGGCAGAGAACTCAGAAAAGAATAAGATGGAACCCAGAAACCTAGCGATCGTTTTTGG TCCAACTCTAGTGAGAACATCAGAAGACAACATGACCCACATGGTCACGCACATGCCTGACCAGTATAAG ATCGTAGAGACTCTCATCCAGCATCATGACTGGTTTTTCACAGAAGAAGGTGCCGAAGAGCCTCTTACAG CAGTGCAGGAGGAGAACACAGTAGACTCCCAGCCAGTACCAAACATAGATCATTTGCTCACCAACATTGG AAGGACAGGCGTGCTCCCTGGAGATGTATCAGATTCAGCTACTAGTGACTCCGCAAAATCTAAGGGCTCC TGGGGATCCGGGAAGGATCAGTATAGCAGGGAACTGCTTGTATCCTCCATCTTCGCAGCTGCCAGTCGCA AGAGGAAAAAGCCGAAAGAGAAAGCCCAGCCCAGCAGCTCTGAGGATGAGCTGGACAGTGTGTTCTTTAA GAAGGAGAATACGGAACAGAGTCACAGTGAGATTAAAGAAGAGTCCAAAAGAGAAAGCGAGACATCAGGT AGCAAGCAGAGGGTCGTCGTTGCCAAAGAAAGTAACACTAAGAAAGACTCGGGTACAACGAAGGAGGAGA AGAAGATACCGTGGGAAGAACCATCCCCACCACACAGCTCCAAACGCAACAGGTCACCAACCCTCAGCTG TCGCCTGGCCATGCTGAAAGAGGGCCCCAGATCACTTCTGACACAGAAGCCCCACTGTGAAGAGACAGGG TCTGACTCAGGCACTCTGCTTAGCACGTCTTCCCAGGCTTCGCTCTTGAGGTCCTCCACTAAAAAATCCA CCAGTCCGGAAACCAAGCACAGTGAGTTTTTGTCCATTGCTGGTACCACCACCTCGGATTATTCTACCAC ATCATCCACTACCTACTTGACCAGCCTGGACTCCAGCCGCCTGAGCCCTGAGGTGCAGTCAGTGGCCGAG AGCAAGGGGGATGAGGCTGATGACGAGAGGAGCGAGCTGGTGAGTGAAGGACGGCCAGTGGAGACCGACA GTGAGAGTGAGTTCCCCGTGTTTCCCACCACCCTGACTTCAGACCGGCTGTTCCGAGGAAAATTCCAGGA GGTTGCCAGGGTGAGCCGCAGGAACTCCGAAGGCAGTGAGGCCAGCTGCACCGAGGGAAGCCTCACACCG AGTTTAGACAGCCGCAGGCAGCAGTTCAGCTCCCATAGACTGATCGAATGCGACACACTGTCCAGGAAGA AGTCTGCTCGCTTCAAGTCAGACAGTGGGAGCCCCGGAGACACCAGGACTGAGAAAGAAACTCCTGCCCT GGCCAAGATGTTTGACGTCATGAAGAAAGGCAAGTCGACAGGGAGCCTCCTCACACCCAGCAGAAGTGAA TCCGAAAAGCAGGAGGCCACTTGGAAAACAAAAATAGCAGATCGTTTAAAACTGAGACCCAGGGCACCCG CAGACGACATGTTTGGAGTTGGGAATCAAAAACCTACTGCTGAGACAGCGAAAAGGAAAAACATCAAACG CAGACACACGCTTGGAGGCCACAGAGATGCTACTGAGATCAGCGTCCTGAGTTTCTGGAAAGCTCATGAG CAGAGTGCGGACAAAGAGTCTGAACTTTCGGCTGTGAATCGGTTAAAACCCAAATGCTCAGCCCAGGACC TTTCCATCTCAGACTGGCTCGCCAGGGAGCGCGTCCGCACCAGTGCCTCCGACCTTAGCAGAGGCGAAGG CCTCGAGCCCCAGGCTGAGAGCCCCAGCGTGCTGGGAACACCCATCAGCACCCACTCACCACCGTCTCAG CAGCCCGAAGCAAGGGTTGCTGCTACCAGCACCTTGGCTTCAACCAGCCAGTCCCCTCTTTTCACACCAC CACAGTCACCTGACCAAATAAACAGAGAAAGCTTCCAGAACATGAGCCAAAATGCTAGTTCTACAGCTAA CATCCACCCCCACAAACAGTCTGAAAGTCCAGACACTAAAGCAGAGACTCCTCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR215805 representing NM_001128084 Red=Cloning site Green=Tags(s) MMATHWTGLPEEDGDKLKACGAASACEVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTL RHFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIG KTYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCY PWLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTV IVPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGIL LKSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSL RAASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPP VSFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRG SNLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSL KVPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQL SENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETG SDTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVP SIATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGI KITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDR REQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDT GVTNRDLISRRIKEYNSLLSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSP PKDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVP GNNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRL RTLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYK IVETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGS WGSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSG SKQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETG SDSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAE SKGDEADDERSELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTP SLDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSE SEKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHE QSADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQ QPEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001128084
ORF Size	5865 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001128084.2, NP_001121556.2
RefSeq Size	6974 bp
RefSeq ORF	5868 bp
Locus ID	71435
MW	217 kDa
Gene Summary	Functions as a GTPase-activating protein (GAP) for RHOA and CDC42. Downstream partner of ARF1 which may control Golgi apparatus structure and function. Also required for CTNNA1 recruitment to adherens junctions (By similarity).[UniProtKB/Swiss-Prot Function]

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