March1 (NM_001166375) Mouse Tagged ORF Clone
CAT#: MR215930
- TrueORF®
41334 (Myc-DDK-tagged) - Mouse membrane-associated ring finger (C3HC4) 1 (March1), transcript variant 2
ORF Plasmid: tGFP
"NM_001166375" in other vectors (3)
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CNY 2,760.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2900024D24Rik; BB085186 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR215930 representing NM_001166375
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCTCACTATGAGCAACATGACCAGCAGCCACATTTGCTGTAATTTTTTAAATATGTGGAAAAAAA GCAAGATATCAACCATGTATTACCTTAATCAAGATGCCAAATTATCTAACTTGTTTCTCCAGGCAAGCAG CCCAACGACAGGGACAGCTCCCAGGAGCCAGTCAAGGTTGTCTGTCTGTCCATCTACTCAGGACATCTGC AGGATCTGTCACTGCGAAGGAGATGAAGAGAGCCCACTCATCACACCCTGTCGCTGCACAGGAACCTTGC GCTTTGTCCACCAGTCCTGCCTCCACCAATGGATCAAGAGCTCAGACACACGATGCTGTGAGCTCTGCAA GTATGACTTCATAATGGAGACCAAGCTCAAGCCCCTTCGGAAGTGGGAAAAGCTCCAGATGACCACGAGC GAAAGGAGGAAAATATTCTGCTCTGTCACGTTCCACGTCATCGCCGTCACCTGTGTGGTGTGGTCCTTGT ATGTGTTGATAGATCGGACAGCGGAGGAAATCAAGCAAGGTGTGCTGGAATGGCCATTTTGGACAAAACT GGTTGTGGTGGCTATTGGCTTCACGGGAGGTCTCGTCTTCATGTATGTACAGTGTAAAGTCTACGTCCAA CTGTGGCGCCGGCTGAAGGCCTACAACCGTGTGATCTTTGTGCAGAATTGCCCAGACACTGCCAACAAAC TGGAGAAGAACTTCCCGTGTAATGTGAACACGGAAATCAAGGATGCTGTGGTTGTGCCTGTGCCACAGAC AGGTTCAAATACACTGCCAACTGCAGAGGGAGCCCCACCTGAGGTTATACCAGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR215930 representing NM_001166375
Red=Cloning site Green=Tags(s) MNLTMSNMTSSHICCNFLNMWKKSKISTMYYLNQDAKLSNLFLQASSPTTGTAPRSQSRLSVCPSTQDIC RICHCEGDEESPLITPCRCTGTLRFVHQSCLHQWIKSSDTRCCELCKYDFIMETKLKPLRKWEKLQMTTS ERRKIFCSVTFHVIAVTCVVWSLYVLIDRTAEEIKQGVLEWPFWTKLVVVAIGFTGGLVFMYVQCKVYVQ LWRRLKAYNRVIFVQNCPDTANKLEKNFPCNVNTEIKDAVVVPVPQTGSNTLPTAEGAPPEVIPV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001166375 |
ORF Size | 825 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001166375.1, NP_001159847.1 |
RefSeq Size | 3942 bp |
RefSeq ORF | 828 bp |
Locus ID | 72925 |
UniProt ID | Q6NZQ8 |
MW | 31.8 kDa |
Gene Summary | E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG215930 | 41334 (tGFP-tagged) - Mouse membrane-associated ring finger (C3HC4) 1 (March1) transcript variant 2, (10ug) |
CNY 4,240.00 |
|
MR215930L3 | Lenti ORF clone of 41334 (Myc-DDK-tagged) - Mouse membrane-associated ring finger (C3HC4) 1 (March1), transcript variant 2 |
CNY 4,660.00 |
|
MR215930L4 | Lenti ORF clone of 41334 (mGFP-tagged) - Mouse membrane-associated ring finger (C3HC4) 1 (March1), transcript variant 2 |
CNY 4,660.00 |