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Dstyk (NM_172516) Mouse Tagged ORF Clone
CAT＃: MR215935
- TrueORF®
Dstyk (Myc-DDK-tagged) - Mouse dual serine/threonine and tyrosine protein kinase (Dstyk)
ORF Plasmid: tGFP
"NM_172516" in other vectors (4)
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CNY 6,792.00
CNY 8,170.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A930019K20Rik; C430014H23Rik; C820013G01; Ripk5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR215935 representing NM_172516
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCCGACGGGCAGTCATGGGCCGGCGAGTCCGTGTCGGGCCCGGGTCCCGGAGGCGGCGGAATGA TCCGCGAGCTGTGCCGGGGCTTCAGCCGCTACCGCCGCTACCTGGGACGGCTGCGGCAGAACCTGCGAGA GACCCAGAAGTTCTTCCGCGACATCAAGTGCTCCCACAGCCACAGTTGTCCCTCCTCCCCCGCGGGCGGC GGAGCGGCCGAGCTTGGCCCTGCCGGCGACGTCGCCGAAGCCCCGCTGCCGGCGGGCCAGCTGAGCTGCA TTTCCTTCCCACCCATGGAAGAGACGTATCTGCAGCAGCTTGTGGACCGCCTTCCCTGTATACTGATCCT CGGCCAGGACTGCAACGCCAAGTGCCAGCTGCTGAACCTGCTGTTGGGGGTGCAGGTGCTTCCCACCCTC AAGCTGGACAGTGATGAGAGCTGTAAGCTTCGTCGCCTCCGCTTCACCTATGGGACTCGGACTCGGGTCA GCTTGGCGCTCCCCGGTCAGTATGAACTAGTGCACACGCTAGCTTCACATCAGGACAACTGGGAGACTAT CCCTGAGGAGGACCTGGAGGTTCAAGAAGACAGTGAGGACGCTGCTCATGTGCTAGCTGACCTCGAGGTG ACAATGCATCATGCTCTCCTGCAGGAAGTGGACATTGTGGTGGCACCGTGCCCGAGCCACCGGCCCTCAG TAGATGTTCTGAGTGACTTGGCAAATGACTTCTTGCCTGTGATAACCTATGCACTTCACAAGGATGAACT CTCTGAGCGGGGTGAGCAAGAGCTTCGGGAAGTTAGACAGTATTTCTCCTTCCCCATGTTCTTTTTCAAA GTACCCAAGCTGGAGATAATCAGCTCTTCCAGTGGGAGAGCAGAGAGTGAAAGGTCACCACTGTATGGAC AGCTGGTGGACCTGGGCTACCTGAGCAGCAGTCACAGGAACTGTGTGCCCTCTGACCAGGACTGTAAAGC TCAGAGCATGTTGGTGGAGCAAAGTGAGAAGCTGAAACAACTGAGTACATTTTCTCACCAGCTGCTACAG AACCGCCTAGTGGATGCAGCCAAGGCCCTGAATGTGGTGCACAGCCACTGCCTTGACATCTTTATTAACC AGGCTTTTGACATGCAGCGGGACCTGCAGATTACTCCCAAACGCCTAGAATATACTAGAAAAAAGGAGAA TGAATTGTATGAATCGTTGATGAATATTGCCAACCGAAAGCAAGAGGAAATGAAGGATATGATTGTTGAG ACACTTAACACCATGAAAGAGGAACTTCTGGATGATGCTGCCAACATGGAGTTTAAAGATGTCATTGTCC CTGAGAATGGAGAAACAATCGGGACCAGGGAGATCAAATCCTGCATCCGCCAGATCCAGGAGCTCATCAT CTCCAGGCTCAATCAGGCCGTGGCCAACAAGCTGATCAGCTCAGTGGACTACCTGCGGGAAAGCTTTGTT GGGACCCTGGAGCGGTGTCTGCAGAGCCTTGAAAAATCACAGGATGTCTCAGTTCATATTACCAGCAACT ATCTCAAACAGATCTTAAATGCCGCTTACCATGTTGAGGTCACTTTTCACTCGGGATCCTCAGTTACTCG GATGCTTTGGGAACAAATCAAGCAGATCATCCAGCGTATCACATGGGTGAACCCACCCACCATCACTCTA GAGTGGAAGAGGAAGGTGGCCCAGGAAGCCATCGACAGCCTCAGCGCCTCCAAGTTGGCCAAGAGCATTT GCAGCCAGTTCCGCACGCGGCTCAATAGTTCTCATGAGGCGTTTGCAGCCTCCTTGAGGCAGCTGGAAGC TGGCCATTCTGGCCGTCTGGAGAAAACTGAAGATCTGTGGCTAAAGGTTCGAAAAGATCACGCCCCTCGT CTGGCCCGCCTTTCGCTAGAGAGCCGGTCTTTACAGGATGTCTTGCTTCACCGTAAACCTAAATTAGGAC AGGAACTAGGCCGGGGCCAATATGGTGTGGTATACCTGTGTGACAACTGGGGGGGACACTTCCCTTGTGC CCTCAAATCAGTTGTTCCTCCAGATGAGAAGCACTGGAACGATCTGGCATTGGAGTTTCATTACATGAGG TCTCTGCCAAAGCATGAGCGGCTGGTCGATCTTCATGGCTCAGTCATTGACTACAACTATGGTGGCGGCT CCAGCGTTGCTGTACTGCTTATCATGGAGCGGCTACACCGGGACCTCTACACAGGGCTGAAGGCTGGGCT GACCCTGGAGACACGTTTGCAAATAGCTCTGGATGTGGTGGAGGGAATCCGCTTTCTGCACAGCCAGGGG CTTGTTCATCGGGATATCAAACTGAAAAATGTGCTGCTGGACAAACAAAACCGTGCCAAGATCACCGACT TAGGATTCTGTAAACCAGAAGCTATGATGTCAGGCAGCATTGTGGGGACACCAATCCATATGGCCCCTGA ACTTTTCACAGGAAAGTATGATAACTCTGTGGACGTCTATGCTTTTGGGATCCTTTTTTGGTATATCTGC TCCGGCTCTATCAAGCTCCCTGAGGCATTTGAGAGGTGTGCTAGCAAAGACCATCTCTGGAATAATGTGC GCAGAGGGACCCGTCCAGAGCGTCTTCCTGTGTTTGATGAGGAGTGCTGGCAGTTGATGGAGGCGTGTTG GGATGGTGACCCCTTGAAGAGGCCCCTCTTGGGCATCGTGCAGCCTATGCTCCGAAGCATTATGGACCGG CTCTGCAAGTGCAGCTCTGAACAACCAAACAGAGGACTAGACGATTCTACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR215935 representing NM_172516
Red=Cloning site Green=Tags(s) MEADGQSWAGESVSGPGPGGGGMIRELCRGFSRYRRYLGRLRQNLRETQKFFRDIKCSHSHSCPSSPAGG GAAELGPAGDVAEAPLPAGQLSCISFPPMEETYLQQLVDRLPCILILGQDCNAKCQLLNLLLGVQVLPTL KLDSDESCKLRRLRFTYGTRTRVSLALPGQYELVHTLASHQDNWETIPEEDLEVQEDSEDAAHVLADLEV TMHHALLQEVDIVVAPCPSHRPSVDVLSDLANDFLPVITYALHKDELSERGEQELREVRQYFSFPMFFFK VPKLEIISSSSGRAESERSPLYGQLVDLGYLSSSHRNCVPSDQDCKAQSMLVEQSEKLKQLSTFSHQLLQ NRLVDAAKALNVVHSHCLDIFINQAFDMQRDLQITPKRLEYTRKKENELYESLMNIANRKQEEMKDMIVE TLNTMKEELLDDAANMEFKDVIVPENGETIGTREIKSCIRQIQELIISRLNQAVANKLISSVDYLRESFV GTLERCLQSLEKSQDVSVHITSNYLKQILNAAYHVEVTFHSGSSVTRMLWEQIKQIIQRITWVNPPTITL EWKRKVAQEAIDSLSASKLAKSICSQFRTRLNSSHEAFAASLRQLEAGHSGRLEKTEDLWLKVRKDHAPR LARLSLESRSLQDVLLHRKPKLGQELGRGQYGVVYLCDNWGGHFPCALKSVVPPDEKHWNDLALEFHYMR SLPKHERLVDLHGSVIDYNYGGGSSVAVLLIMERLHRDLYTGLKAGLTLETRLQIALDVVEGIRFLHSQG LVHRDIKLKNVLLDKQNRAKITDLGFCKPEAMMSGSIVGTPIHMAPELFTGKYDNSVDVYAFGILFWYIC SGSIKLPEAFERCASKDHLWNNVRRGTRPERLPVFDEECWQLMEACWDGDPLKRPLLGIVQPMLRSIMDR LCKCSSEQPNRGLDDST TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172516 |
| ORF Size | 2781 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172516.4, NP_766104.2 |
| RefSeq Size | 6385 bp |
| RefSeq ORF | 2784 bp |
| Locus ID | 213452 |
| UniProt ID | Q6XUX1 |
| MW | 105.4 kDa |
| Gene Summary | Acts as a positive regulator of ERK phosphorylation downstream of fibroblast growth factor-receptor activation. Involved in the regulation of both caspase-dependent apoptosis and caspase-independent cell death. In the skin, it plays a predominant role in suppressing caspase-dependent apoptosis in response to UV stress in a range of dermal cell types.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222661 | Dstyk (untagged) - Mouse dual serine/threonine and tyrosine protein kinase (Dstyk), (10ug) |
CNY 11,880.00 |
|
| MG215935 | Dstyk (tGFP-tagged) - Mouse dual serine/threonine and tyrosine protein kinase (Dstyk), (10ug) |
CNY 9,030.00 |
|
| MR215935L3 | Lenti ORF clone of Dstyk (Myc-DDK-tagged) - Mouse dual serine/threonine and tyrosine protein kinase (Dstyk) |
CNY 10,070.00 |
|
| MR215935L4 | Lenti ORF clone of Dstyk (mGFP-tagged) - Mouse dual serine/threonine and tyrosine protein kinase (Dstyk) |
CNY 10,070.00 |
