Dusp7 (NM_153459) Mouse Tagged ORF Clone
CAT#: MR215943
- TrueORF®
Dusp7 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 7 (Dusp7)
ORF Plasmid: tGFP
"NM_153459" in other vectors (4)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AU015694; Mkpx; Pyst2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR215943 representing NM_153459
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAAACCAGCTCCGCGGGCCCCCAGTGCGGGCGCACATGTCGACTTCGGGGGCGGCGGCGGCGGCGG CGGCTGGGGGCACTCGGGCGGGGTCGGAGCCCGGTGCGGGATCTGGGTCCGGCGCAGGCATCGGAGCGGG AGCGACGACGGGAGCGGGGGCCATGCCCTGCAAGAGCGCCGAGTGGCTGCAGGAGGAGCTGGAGGCGCGC GGTGGCGCGTCCCTGCTGCTGCTAGATTGCCGGCCGCACGAGCTCTTCGAATCGTCGCACATTGAGACGG CCATCAACCTGGCCATCCCGGGTCTCATGCTGCGCCGCCTGCGCAAAGGCAACCTGCCCATCCGCTCCAT CATTCCCAACCATGCCGACAAGGAGCGCTTCGCCACGCGCTGCAAGGCGGCCACCGTGCTGCTCTACGAC GAGGCCACGGCGGAGTGGCAGCCCGAGCCCGGCGCCCCCGCCTCCGTTCTCGGCCTGCTCCTGCAGAAGC TGCGAGACGACGGCTGCCAGGCCTACTACCTCCAAGGTGGTTTCAACAAGTTCCAGACGGAATACTCCGA GCATTGTGAGACTAACGTGGACAGCTCGTCCTCCCCGAGTGGCTCGCCACCCACCTCCGTGCTGGGCCTG GGGGGCCTGCGCATCAGCTCTGACTGCTCAGACGGCGAGTCGGACCGAGAGCTGCCCAGCAGTGCCACTG AGTCGGATGGCAGCCCTGTACCATCCAGCCAGCCGGCCTTTCCAGTCCAGATCCTGCCCTACCTCTACCT CGGCTGTGCCAAGGACTCTACCAACCTGGATGTACTTGGCAAATACGGCATCAAATACATCCTCAACGTC ACACCCAACCTGCCCAACGCCTTTGAGCACGGTGGTGAGTTCACCTACAAGCAAATCCCCATCTCTGACC ACTGGAGCCAGAACCTCTCTCAGTTCTTCCCCGAGGCCATCAGCTTCATTGATGAAGCCCGCTCCAAGAA GTGTGGTGTCTTGGTGCACTGCCTAGCAGGCATCAGCCGTTCAGTGACAGTTACTGTAGCCTACCTGATG CAGAAGATGAACCTGTCCCTCAACGACGCCTATGACTTCGTCAAGAGGAAAAAGTCTAACATCTCGCCCA ACTTCAACTTCATGGGGCAGCTGCTGGACTTTGAGCGGACGCTGGGGCTCAGCAGCCCATGCGACAACCA CGCACCCAGTGAGCAGCTCTACTTCTCCACGCCCACCAACCATAACCTGTTCCCGATCAACACGCTCGAG TCCACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR215943 representing NM_153459
Red=Cloning site Green=Tags(s) MKNQLRGPPVRAHMSTSGAAAAAAAGGTRAGSEPGAGSGSGAGIGAGATTGAGAMPCKSAEWLQEELEAR GGASLLLLDCRPHELFESSHIETAINLAIPGLMLRRLRKGNLPIRSIIPNHADKERFATRCKAATVLLYD EATAEWQPEPGAPASVLGLLLQKLRDDGCQAYYLQGGFNKFQTEYSEHCETNVDSSSSPSGSPPTSVLGL GGLRISSDCSDGESDRELPSSATESDGSPVPSSQPAFPVQILPYLYLGCAKDSTNLDVLGKYGIKYILNV TPNLPNAFEHGGEFTYKQIPISDHWSQNLSQFFPEAISFIDEARSKKCGVLVHCLAGISRSVTVTVAYLM QKMNLSLNDAYDFVKRKKSNISPNFNFMGQLLDFERTLGLSSPCDNHAPSEQLYFSTPTNHNLFPINTLE ST TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_153459 |
ORF Size | 1266 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_153459.4, NP_703189.3 |
RefSeq Size | 3227 bp |
RefSeq ORF | 1269 bp |
Locus ID | 235584 |
UniProt ID | Q91Z46 |
MW | 45.7 kDa |
Gene Summary | Dual specificity protein phosphatase (PubMed:27783954). Shows high activity towards MAPK1/ERK2 (By similarity). Also has lower activity towards MAPK14 and MAPK8 (By similarity). In arrested oocytes, plays a role in meiotic resumption (PubMed:27783954). Promotes nuclear envelope breakdown and activation of the CDK1/Cyclin-B complex in oocytes, probably by dephosphorylating and inactivating the conventional protein kinase C (cPKC) isozyme PRKCB (PubMed:27783954). May also inactivate PRKCA and/or PRKCG (PubMed:27783954). Also important in oocytes for normal chromosome alignment on the metaphase plate and progression to anaphase, where it might regulate activity of the spindle-assembly checkpoint (SAC) complex (PubMed:27783954).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC213009 | Dusp7 (untagged) - Mouse dual specificity phosphatase 7 (Dusp7), (10ug) |
CNY 3,656.00 |
|
MG215943 | Dusp7 (tGFP-tagged) - Mouse dual specificity phosphatase 7 (Dusp7), (10ug) |
CNY 3,710.00 |
|
MR215943L3 | Lenti ORF clone of Dusp7 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 7 (Dusp7) |
CNY 5,230.00 |
|
MR215943L4 | Lenti ORF clone of Dusp7 (mGFP-tagged) - Mouse dual specificity phosphatase 7 (Dusp7) |
CNY 5,230.00 |