Tnn (NM_177839) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	tenascin-W; TN-W; Tnw
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR215968 representing NM_177839, codon optimized. Due to the complexity of NM_177839, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACTGTGGGGAATGCTCGCATTCCCCCTGGGCTTTTTGCTCGCCTCCGTGCTGCTTGTCGCCAGCG CGCCCGCAACACCAGAATCTCCAGGCTGCTCCAACAAGGAGCAGCAGGTCACTGTGTCCCATACATACAA GATAGATGTGCCTAAATCTGCCCTCGTGCAGGTTGAGACAGATCCTCAGAGTCTCAGTGACGACGGAACA AGCCTGCTGGCTCCTGGCGAAGACGGAGAGGAGCAGAACATAATCTTCAGACATAACATCAGACTGCAGA CCCCACAGAAAAACTGCGATCTTGCGGACAGTGTCCAGGATCTGTTGGCCAGGATGAAAAAGCTGGAGGA AGAGATGGCTGAGTTGAAGGAGCAATGTAATACCAACAGATGCTGCCAGGGGGCCGCGGACTTGTCCAGA CATTGCTCTGGCCACGGCACCTTTCTGCCTGAAACATGCTCCTGCCATTGCGACCAAGGCTGGGAGGGAG CCGATTGCGATCAGCCAACATGCCCCGGGGCTTGCAACGGACACGGACGATGCGTCGATGGACAGTGCGT GTGCGATGCTCCCTATGTAGGAGTGGACTGCGCTTACGCCGCGTGTCCCCAGGATTGTTCAGGGCATGGA GTGTGTGTCCAAGGTGTCTGTCAGTGCCACGAGGACTTCACAGCTGAGGACTGCTCCGAGCAGAGGTGCC CGGGAGATTGCAGTGGGAATGGTTTCTGCGATACTGGTGAGTGCTATTGCGAGATGGGGTTTACCGGGCC GGACTGCAGTCAGGTGGTAGCCCCACAAGGACTGCAGCTGCTGAAGAGCACCGAGAACTCTCTGCTCGTG AGCTGGGAGCCCTCCAGTGAGGTAGACTATTACCTCCTCTCTTACTATCCTCTGGGCAAGGAGCAGGCTA CTAAACAGGTGCGGGTTCCTAAAGAGCAGCATACATACGATATTACAGGGCTGTTGCCCGGGACCAAGTA TATCGTCACACTTCGAAACGTAAAGAAGGACATCAGCTCAAGTCCCCAGCACCTGCTGGCAACAACCGAT CTCGCAGTCGTAGGCACCGCCTGGGTTAACGAAGAAACAGAAACTTCTCTTGACGTGGAATGGGAGAACC CACTTACGGAAGTCGACTATTATAAGCTCAGATATGGGCCCCTGACAGGCCAGGAAGTGACTGAAGTCAC GGTCCCAAAAAGCAGAGATCCAAAAAGTCGCTATGATATCACAGGCCTGCAGCCAGGGACCGAATATAAA ATCACTGTGGTGCCCATCCGAGGCGATCTGGAGGGCAAGCCAATACTGTTGAATGGGCGGACCGAAATCG ATGGCCCGACGAATGTGGTGACAAACCAAGTGACGGAGGATACCGCTTCCGTGTCTTGGGACCCCGTCAG AGCTGATATCGATAAGTATGTAGTGCGGTACATCGCACCTGATGGGGAAACTAAGGAGAAAGCAGTGCCA AAAGACCAGAGTAGTACGGTGCTCACTGGACTTAAACCCGGTGAAGCTTATAAAGTGTTTGTCTGGGCCG AACGAGGCAATCAGGGCTCAAAGAAGGCAGACACAAAGGCATTGACCGAAATTGACTCCCCTGAAAATCT GGTGACAGATAGGGTCACTGAGAACAGTCTTAGCGTCTCATGGGACCCCGTGGAAGCAGATATCGACCGC TATGTGGTGAGCTACACTTCCGTCGATGGCGAAACCAAGCAGGTCCCAGTCAAAAAGGATCAGCGATCAA CCGTGCTGACAGGGTTGTCTCCAGGCGTCGAGTATAAAGTGTACGTGTGGGCTGAGAAGGGTGATAGGGA GAGCAAGAAGGCCAACACCAAGGCTCCAACAGACATTGATTCTCCCAAGAACCTCGTCACTGACCAGGTC ACCGAAAACACACTGTCTGTTTCTTGGGATCCTGTACAAGCAAACATCGATAGGTATATGGTGTCATATA CCAGCGCTGACGGAGAAACACGGGAGGTGCCTGTGCCCAAAGAAAAATCCTCCACCGTCCTGACAGGCCT TCGCCCAGGCGTGGAGTATAAGGTCCACGTCTGGGCTCAGAAGGGCACTCAGGAATCACGCAAAGCAAAC ACTAAAGCTCCCACCGATATTGATGGGCCTAAGAACTTGGTGACAGATCAGGTTACTGAAACCACGCTCT CAGTCAGCTGGGACCCCGTAGAGGCTGATATCGACAGATACATGGTGCGGTATACATCTCCTGACGGTGA AACAAAGGAAGTGCCAGTTTCCAAAGACAAATCTTCTACAGTTCTCAGGGGGCTGCGCCCTGGCGTCGAA TACAAGGTCGATGTATGGGCTCAGAAGGGAGCACAGGATAGCAGGAAGGCGAATACTAAGGCGCCCACAG ATATCGACTCCCCAAAGAATTTGGTGACCGAGCAAGTCACTGAGAGTACAGCAACTGTTAGCTGGGACCC TGTGGAAGCAGATATTGACCGGTACGTCGTCCGGTACACCTCCGTCGATGGCGAAACGCGAGAATTCTTG GTGGGCAAAGATCAGACCTCTACGGTTTTGACAGGCATGCGGCCAGGCGTTGAGTACCAAGTCGATGTCT GGGCACAGAAGGGGACCCAGGAGAGTAGAAAGACTAGCACTAAGGCCCCAACAGACATTGACGGGCCTAA AAATTTGGTTACTGACCAAGTCACCGAGACTACACTGAGTGTATCTTGGGACCCAGTTGAGGCCGATATC GATAGGTACATGGTTCGGTACACCTCCCCCGATGGGGAGACGAAAGAGGTGCCAGTTTCCAAGGATAAGT CAAGCACAGTCCTCCGAGGTTTGAGGCCTGGAGTAGAATACAAGGTTGACGTGTGGGCTCAGAAAGGAGC ACAAGATTCCCGGAAGGCCAACACCAAAGCACCGACCGATATAGACTCACCCAAAAACCTCGCAATCGAT CAGGTCACCGAGACAACCCTGTCAGTATCCTGGGATCCTGTGCAGGCTGATATCGATAGGTACGTGGTGA GATACACGAGCGCTGACGGGGAAAGCAAAGAGTTCTTGATCGGCAAAGAACAGCGAAGTACTGTGCTTAC GGGACTTAGGCCAGGCGTCGAATATAAGGTCGAGGTCTGGGCCCAGAAGGGGGCCCGGGAGTCCAAGAAA GCAAATACAGAAGGTCACACTGACATTGACTCTCCTAAGAATCTGGTCACCAATCAGGTGACCGAGAATA CAGCTACCATTTCTTGGGATCCAGTGCAGGCCGATATTGACCGCTACATGGTGCGGTATACGTCTGCCGA CGGAGAGACTAGAGAGATCCCGGTCCGAAAAGAGAAGTCATCTACCGTCCTCACAGGTCTGCGACCCGGG GTCGAGTACACAGTGCAGGTATGGGCCCAGAAAGGCGCCCGCGAATCCAAAAAAGCCAAGACCAAGGCCC CCACCGAAATTGACTCCCCCAAAAATCTCGTCACTAACCGGGTTACGGAAAATACAGCCACCATAAGTTG GGACCCTGTGAGGGCTAATATTGATAGGTACATGGTCCGGTATACCTCTGCCGATGGGGAAACAAAGGAG ATACCCGTCTCAAAGGACCAGAGTTCCACTATACTGACCGGTCTGAAGCCTGGGATGGAGTATACAATCC ACGTCTGGGCTCAGAAAGGCGCACGGGAGTCTAAGAAAGCCGACACTAAGGCGTTGACCGAGATTGATCC CCCAAGAAACCTCCGACCTTTCGGGGTAACTCACAGCGGGGGCGTCCTGACATGGCTCCCACCATCCGCC CAAATCGATGGCTACATTTTGACATACCAGTTTCCTAATGGGACCGTCAAAGAAGTGGAGCTGCCTCGAG GTCAGCAGCGGTTCGAGCTGCAAGATCTGGAGCAAGGCGTCACATACCCCGTGTCCCTTGTGGCCTTCAA GGGTAACCAACGGAGCCGAACTGTTAGTACTACACTCTCTACCGTAGATGCCCGGTTTCCACATCCATCT GACTGTTCACAGGTGCAGCAGAACACAAATGCCGCCTCCGGTCTGTACACCATCTATCTGAACGGGGACG CCAGTAGGCCTATGCAAGTATACTGCGACATGGATACTGACGGTGGTGGGTGGATCGTTTTTCAGAGAAG AAATACCGGCCAACTGGACTTTTTTAAAAGATGGAGGAGTTACGTGGAAGGCTTCGGCGACCCTATGAAG GAGTTTTGGCTGGGACTGGATAAGCTGCACAACCTGACTACAGGAACTACTACAAGGTACGAGGTGAGAG CCGACCTCCAGACGTTCAACGAAAGTGCTTACGCAGTGTACGATTTTTTTCAGGTTGCATCTAGTAAAGA GCGGTATAAGTTGTCCGTAGGAAAATACAGAGGTACAGCAGGCGACGCACTGACCTATCACAACGGATGG AAGTTCACTACTTTTGACAGGGATAACGACATCGCACTCTCCAACTGTGCTCTGACTCACCACGGTGGAT GGTGGTACAAAAATTGTCACTTGGCTAATCCAAACGGAAAGTATGGGGAGACTAAACATAGCGAGGGCGT CAACTGGGAACCTTGGAAGGGACACGAATTTTCAATCCCGTATGTGGAGCTCAAAATCAGGCCCTTCGGG TACAGCCGGGACCGATTCTCTGGGCGCAAGAAAAGGTCAATTGGGAAAGCTCGGATGTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR215968 representing NM_177839 Red=Cloning site Green=Tags(s) MGLWGMLAFPLGFLLASVLLVASAPATPESPGCSNKEQQVTVSHTYKIDVPKSALVQVETDPQSLSDDGT SLLAPGEDGEEQNIIFRHNIRLQTPQKNCDLADSVQDLLARMKKLEEEMAELKEQCNTNRCCQGAADLSR HCSGHGTFLPETCSCHCDQGWEGADCDQPTCPGACNGHGRCVDGQCVCDAPYVGVDCAYAACPQDCSGHG VCVQGVCQCHEDFTAEDCSEQRCPGDCSGNGFCDTGECYCEMGFTGPDCSQVVAPQGLQLLKSTENSLLV SWEPSSEVDYYLLSYYPLGKEQATKQVRVPKEQHTYDITGLLPGTKYIVTLRNVKKDISSSPQHLLATTD LAVVGTAWVNEETETSLDVEWENPLTEVDYYKLRYGPLTGQEVTEVTVPKSRDPKSRYDITGLQPGTEYK ITVVPIRGDLEGKPILLNGRTEIDGPTNVVTNQVTEDTASVSWDPVRADIDKYVVRYIAPDGETKEKAVP KDQSSTVLTGLKPGEAYKVFVWAERGNQGSKKADTKALTEIDSPENLVTDRVTENSLSVSWDPVEADIDR YVVSYTSVDGETKQVPVKKDQRSTVLTGLSPGVEYKVYVWAEKGDRESKKANTKAPTDIDSPKNLVTDQV TENTLSVSWDPVQANIDRYMVSYTSADGETREVPVPKEKSSTVLTGLRPGVEYKVHVWAQKGTQESRKAN TKAPTDIDGPKNLVTDQVTETTLSVSWDPVEADIDRYMVRYTSPDGETKEVPVSKDKSSTVLRGLRPGVE YKVDVWAQKGAQDSRKANTKAPTDIDSPKNLVTEQVTESTATVSWDPVEADIDRYVVRYTSVDGETREFL VGKDQTSTVLTGMRPGVEYQVDVWAQKGTQESRKTSTKAPTDIDGPKNLVTDQVTETTLSVSWDPVEADI DRYMVRYTSPDGETKEVPVSKDKSSTVLRGLRPGVEYKVDVWAQKGAQDSRKANTKAPTDIDSPKNLAID QVTETTLSVSWDPVQADIDRYVVRYTSADGESKEFLIGKEQRSTVLTGLRPGVEYKVEVWAQKGARESKK ANTEGHTDIDSPKNLVTNQVTENTATISWDPVQADIDRYMVRYTSADGETREIPVRKEKSSTVLTGLRPG VEYTVQVWAQKGARESKKAKTKAPTEIDSPKNLVTNRVTENTATISWDPVRANIDRYMVRYTSADGETKE IPVSKDQSSTILTGLKPGMEYTIHVWAQKGARESKKADTKALTEIDPPRNLRPFGVTHSGGVLTWLPPSA QIDGYILTYQFPNGTVKEVELPRGQQRFELQDLEQGVTYPVSLVAFKGNQRSRTVSTTLSTVDARFPHPS DCSQVQQNTNAASGLYTIYLNGDASRPMQVYCDMDTDGGGWIVFQRRNTGQLDFFKRWRSYVEGFGDPMK EFWLGLDKLHNLTTGTTTRYEVRADLQTFNESAYAVYDFFQVASSKERYKLSVGKYRGTAGDALTYHNGW KFTTFDRDNDIALSNCALTHHGGWWYKNCHLANPNGKYGETKHSEGVNWEPWKGHEFSIPYVELKIRPFG YSRDRFSGRKKRSIGKARMF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_177839
ORF Size	4680 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_177839.1, NM_177839.2, NM_177839.3, NP_808507.2
RefSeq Size	5825 bp
RefSeq ORF	4683 bp
Locus ID	329278
UniProt ID	Q80Z71
MW	173.1 kDa
Gene Summary	Extracellular matrix protein that seems to be a ligand for ITGA8:ITGB1, ITGAV:ITGB1 and ITGA4:ITGB1 (By similarity) (PubMed:14709716). Involved in neurite outgrowth and cell migration in hippocampal explants (PubMed:12812753). During endochondral bone formation, inhibits proliferation and differentiation of proteoblasts mediated by canonical WNT signaling (PubMed:17395156). In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells (By similarity). Expressed in most mammary tumors, may facilitate tumorigenesis by supporting the migratory behavior of breast cancer cells (PubMed:15592496).[UniProtKB/Swiss-Prot Function]

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