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Entpd5 (NM_007647) Mouse Tagged ORF Clone
CAT＃: MR216347
- TrueORF®
Entpd5 (Myc-DDK-tagged) - Mouse ectonucleoside triphosphate diphosphohydrolase 5 (Entpd5), transcript variant 1
ORF Plasmid: tGFP
"NM_007647" in other vectors (3)
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CNY 4,470.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI196558; AI987697; Cd39l4; ER-UDPase; mNTPase; NTPDase-5; NTPDase5; Pcph |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR216347 representing NM_007647
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCTGCCTCCACAGGAGTGTGAGCAGCACTGCTTCAGCAACAAAGCCTCAGGTCCACATCTTGGGA AGAATATGGCCACTTCCTGGGGGGCTGTCTTCATGCTGATCATAGCCTGCGTTGGCAGCACTGTCTTCTA CAGAGAACAGCAGACCTGGTTTGAAGGTGTCTTCTTGTCTTCCATGTGCCCCATTAATGTCAGTGCCGGC ACCTTTTATGGAATTATGTTTGATGCGGGCAGCACTGGAACTCGGATTCATGTTTACACTTTTGTGCAGA AAACAGCAGGACAGCTCCCCTTTCTGGAAGGTGAAATTTTTGATTCTGTGAAGCCGGGACTTTCTGCTTT TGTGGATCAGCCCAAACAGGGTGCTGAGACTGTCCAGGAGCTCTTGGAGGTGGCCAAAGACTCGATCCCC AGAAGCCACTGGGAAAGGACCCCGGTGGTTCTGAAAGCAACGGCCGGACTCCGTTTGCTGCCTGAGCAGA AAGCCCAGGCTCTGCTCTTGGAGGTAGAGGAGATCTTCAAGAATTCACCTTTCCTGGTCCCAGATGGCAG CGTTAGCATCATGGATGGGTCCTATGAAGGCATACTAGCCTGGGTTACCGTGAACTTTCTAACAGGTCAG CTGCATGGTCGTGGCCAGGAGACTGTGGGGACCCTTGACCTGGGGGGTGCCTCCACCCAAATCACGTTTC TACCCCAGTTTGAGAAAACCCTGGAACAAACACCTAGGGGCTACCTCACTTCCTTTGAGATGTTTAACAG CACTTTTAAGCTCTATACACATAGTTACTTGGGATTTGGACTGAAAGCTGCAAGACTGGCAACTCTGGGA GCCCTGGAAGCAAAAGGGACTGATGGACATACGTTTCGAAGTGCCTGTTTACCAAGATGGTTGGAAGCAG AGTGGATCTTTGGGGGTGTGAAATACCAGTATGGTGGTAACCAAGAAGGGGAGATGGGCTTTGAACCCTG CTATGCGGAAGTGCTGAGGGTAGTACAGGGGAAACTTCACCAGCCAGAAGAAGTCCGAGGAAGCGCCTTC TACGCTTTCTCTTACTACTACGATCGAGCCGCTGACACACACTTGATCGATTATGAAAAGGGCGGGGTTT TAAAAGTTGAAGATTTTGAAAGAAAAGCCAGAGAAGTGTGTGACAACTTGGGGAGCTTCTCCTCGGGCAG TCCTTTCCTCTGCATGGACCTCACTTACATCACAGCCCTGTTGAAAGATGGTTTTGGCTTTGCCGACGGC ACCCTCTTACAGCTCACAAAGAAAGTGAACAACATAGAGACTGGTTGGGCCTTGGGGGCCACCTTTCACC TGCTCCAGTCTCTGGGCATCACCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216347 representing NM_007647
Red=Cloning site Green=Tags(s) MTLPPQECEQHCFSNKASGPHLGKNMATSWGAVFMLIIACVGSTVFYREQQTWFEGVFLSSMCPINVSAG TFYGIMFDAGSTGTRIHVYTFVQKTAGQLPFLEGEIFDSVKPGLSAFVDQPKQGAETVQELLEVAKDSIP RSHWERTPVVLKATAGLRLLPEQKAQALLLEVEEIFKNSPFLVPDGSVSIMDGSYEGILAWVTVNFLTGQ LHGRGQETVGTLDLGGASTQITFLPQFEKTLEQTPRGYLTSFEMFNSTFKLYTHSYLGFGLKAARLATLG ALEAKGTDGHTFRSACLPRWLEAEWIFGGVKYQYGGNQEGEMGFEPCYAEVLRVVQGKLHQPEEVRGSAF YAFSYYYDRAADTHLIDYEKGGVLKVEDFERKAREVCDNLGSFSSGSPFLCMDLTYITALLKDGFGFADG TLLQLTKKVNNIETGWALGATFHLLQSLGITS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007647 |
| ORF Size | 1356 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007647.3 |
| RefSeq Size | 4917 bp |
| RefSeq ORF | 1359 bp |
| Locus ID | 12499 |
| UniProt ID | Q9WUZ9 |
| MW | 50.3 kDa |
| Gene Summary | Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. The nucleotide hydrolyzing preference is GDP > IDP > UDP, but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG216347 | Entpd5 (tGFP-tagged) - Mouse ectonucleoside triphosphate diphosphohydrolase 5 (Entpd5) transcript variant 1, (10ug) |
CNY 4,940.00 |
|
| MR216347L3 | Lenti ORF clone of Entpd5 (Myc-DDK-tagged) - Mouse ectonucleoside triphosphate diphosphohydrolase 5 (Entpd5), transcript variant 1 |
CNY 6,370.00 |
|
| MR216347L4 | Lenti ORF clone of Entpd5 (mGFP-tagged) - Mouse ectonucleoside triphosphate diphosphohydrolase 5 (Entpd5), transcript variant 1 |
CNY 6,370.00 |
