Cit (NM_007708) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C030025P15Rik; Cit-k; CRIK; CRIK-SK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR216404 representing NM_007708 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGAAGTTCAAGTATGGTGTGCGGAACCCGCCGGAGGCCAGTGCCTCCGAGCCCATTGCCAGTCGGG CCTCCAGGCTAAATCTCTTCTTCCAGGGGAAACCGCCCCTCATGACTCAACAGCAGATGTCTGCTCTTTC CCGGGAAGGGATGCTAGACGCCCTCTTCGCTCTCTTTGAAGAGTGCAGCCAACCCGCCCTGATGAAGATG AAGCACGTGAGCAGCTTTGTCCAGAAGTATTCCGACACCATAGCCGAGTTGCGGGAGCTGCAGCCGTCGG CGAGAGACTTCGAAGTTCGAAGCCTTGTGGGCTGTGGTCACTTCGCTGAAGTGCAGGTGGTTAGAGAGAA GGCGACCGGGGACGTCTATGCCATGAAAATCATGAAGAAGAAGGCTTTGCTGGCCCAGGAACAGGTTTCA TTTTTCGAGGAGGAGAGGAACATATTATCTCGGAGCACGAGTCCTTGGATCCCCCAGTTACAGTACGCCT TTCAGGACAAAAATAACCTTTACCTGGTCATGGAATATCAGCCTGGAGGGGATTTGCTGTCGCTTCTGAA CAGATACGAGGACCAATTAGATGAGAGCATGATCCAGTTTTACCTTGCTGAGCTGATTTTGGCTGTCCAC AGCGTGCACCAGATGGGATATGTGCATCGAGACATCAAGCCCGAGAACATCCTCATCGACCGGACGGGAC ACATCAAGCTGGTGGATTTTGGATCAGCCGCTAAGATGAATTCAAATAAGGTGGATGCCAAACTCCCCAT TGGGACCCCGGATTACATGGCTCCGGAAGTGTTGACCGTGATGAACGAGGACCGAAGGGGCACATACGGC TTGGACTGTGACTGGTGGTCTGTCGGAGTTGTTGCTTATGAGATGGTTTATGGGAAGACCCCATTCACAG AGGGAACCTCCGCCCGGACCTTCAACAACATCATGAACTTCCAGCGGTTTTTGAAGTTCCCAGATGACCC CAAAGTTAGCAGTGAGCTCCTTGATCTGCTTCAGAGTCTGCTGTGTGTCCAGAAAGAGAGACTGAAGTTC GAGGGTCTCTGCTGCCACCCTTTCTTTGCCAGAACGGACTGGAACAACATCCGTAACTCTCCTCCCCCCT TCGTCCCCACCCTCAAGTCTGACGATGACACCTCCAATTTTGATGAACCAGAGAAGAATTCGTGGGTTTC ATCCTCTGTGTGCCAGCTGAGCCCCTCGGGCTTCTCAGGCGAAGAGCTGCCGTTTGTGGGATTTTCGTAC AGCAAGGCACTGGGGTATCTTGGTAGATCTGAGTCTGTCGTGTCGAGTCTGGACTCCCCTGCCAAGGTTA GCTCCATGGAAAAGAAACTTCTCATCAAAAGCAAAGAGCTCCAAGACTCCCAGGACAAGTGTCACAAGAT GGAGCAGGAAATGACCCGGTTACATCGCAGAGTGTCAGAGGTGGAGGCTGTGCTTAGTCAGAAGGAGGTG GAGCTGAAGGCCTCTGAGACTCAGAGATCCCTCCTGGAGCAGGACCTTGCTACCTACATCACAGAATGCA GTAGCTTAAAGCGAAGTTTGGAGCAAGCGCGGATGGAGGTGTCCCAGGAGGATGACAAAGCTCTGCAGCT TCTCCACGACATCCGAGAGCAGAGCCGGAAGCTCCAGGAGATCAAGGAGCAGGAGTACCAGGCTCAGGTG GAGGAGATGAGGCTGATGATGAATCAGCTGGAAGAAGACCTGGTGTCAGCCCGCAGACGCAGCGATCTCT ACGAGTCTGAGCTGAGGGAGTCTCGGCTTGCCGCCGAGGAATTCAAGCGGAAGGCAAACGAATGTCAGCA CAAACTGATGAAGGCTAAGGACCAAGGGAAGCCTGAAGTGGGAGAATATTCCAAACTGGAGAAGATCAAT GCTGAGCAGCAGCTGAAGATCCAGGAGCTCCAGGAGAAGCTGGAGAAGGCTGTAAAAGCCAGCACAGAGG CCACCGAGCTCCTGCAGAATATCCGCCAGGCAAAGGAGCGAGCAGAGCGGGAGCTAGAGAAGCTACACAA CCGGGAAGACTCCTCCGAGGGCATCAAAAAGAAGCTGGTGGAAGCCGAGGAACGCCGCCACTCCCTGGAG AACAAGGTAAAGAGACTAGAGACCATGGAGCGTAGAGAGAACAGACTGAAGGATGACATCCAGACAAAGT CCGAACAGATCCAGCAGATGGCTGATAAAATTCTGGAGCTGGAGGAGAAACATCGGGAGGCTCAGGTCTC AGCTCAACATCTAGAAGTACACTTGAAACAGAAAGAACAGCACTACGAGGAAAAGATCAAGGTATTGGAC AATCAGATAAAGAAGGACCTGGCCGACAAGGAGAGCCTGGAGAACATGATGCAGAGACACGAGGAGGAGG CCCACGAGAAGGGCAAGATCCTCAGCGAGCAGAAGGCGATGATCAACGCGATGGATTCCAAGATCCGATC CCTGGAGCAGAGGATCGTGGAGCTGTCGGAAGCCAACAAGCTTGCGGCAAACAGCAGTCTCTTCACCCAG AGGAACATGAAGGCCCAGGAAGAGATGATCTCAGAACTCAGGCAGCAGAAATTTTACCTGGAGACGCAGG CCGGAAAGCTGGAGGCCCAGAACCGAAAGCTGGAAGAGCAACTGGAGAAGATCAGCCACCAAGATCACAG TGACAAGAGTCGGCTGCTGGAGCTGGAAACAAGGCTGAGGGAGGTCAGCCTGGAGCACGAGGAGCAGAAG CTGGAGCTGAAGCGGCAGCTCACGGAGCTGCAGCTGTCCCTGCAGGAGCGCGAGTCCCAGCTGACGGCCC TGCAGGCCGCCCGGGCAGCGCTGGAGAGCCAGCTCCGCCAGGCGAAGACAGAGCTGGAGGAGACAACCGC GGAAGCGGAGGAGGAGATCCAGGCGCTCACGGCACATCGAGATGAGATCCAGCGCAAATTCGATGCCCTT CGCAACAGCTGCACGGTCATCACCGACCTGGAGGAGCAGCTGAACCAGCTCACCGAGGACAACGCCGAGC TCAACAACCAAAACTTCTACCTGTCCAAACAACTCGATGAGGCTTCCGGGGCCAATGACGAGATTGTGCA GCTGCGGAGTGAGGTGGACCATCTCCGCCGTGAGATCACGGAGCGGGAGATGCAGCTCACCAGCCAGAAG CAAACGATGGAGGCTCTGAAGACGACATGCACCATGCTGGAGGAGCAGGTCCTGGACCTGGAGGCCCTGA ACGACGAGCTGCTGGAGAAGGAGCGCCAGTGGGAGGCCTGGCGGAGCGTCCTCGGCGACGAGAAGTCCCA GTTCGAGTGTCGAGTTCGAGAGCTACAGAGGATGCTGGACACCGAGAAGCAGAGCAGGGCTAGGGCCGAT CAGCGGATCACCGAGTCGCGCCAGGTGGTGGAGTTGGCGGTGAAGGAACACAAGGCCGAGATTCTTGCTC TGCAGCAGGCTCTCAAGGAGCAGAAGCTCAAAGCCGAGAGCCTGTCGGACAAGCTCAACGACCTGGAGAA GAAGCACGCCATGCTGGAGATGAACGCCCGGAGCTTACAGCAGAAACTAGAGACAGAGCGGGAGCTCAAA CAGAGGCTTCTGGAGGAGCAAGCCAAATTGCAGCAGCAGATGGACCTGCAGAAGAACCACATCTTCAGAC TGACGCAAGGGCTGCAGGAGGCGCTGGACCGGGCGGATCTGCTGAAGACAGAAAGGAGCGACCTGGAGTA CCAGCTGGAAAACATTCAGGTTCTCTACTCTCACGAGAAAGTGAAAATGGAAGGCACAATCTCTCAGCAA ACCAAACTCATTGATTTCCTGCAAGCCAAAATGGACCAGCCTGCTAAAAAGAAAAAGGTTCCTCTGCAGT ACAATGAGCTGAAGCTAGCCCTGGAAAAGGAGAAAGCCCGATGCGCGGAGCTGGAGGAGGCCCTTCAGAA GACCCGCATCGAACTCCGCTCTGCGCGGGAGGAAGCTGCCCACCGCAAAGCCACGGACCACCCGCACCCG TCTACGCCAGCCACTGCGAGGCAGCAGATCGCCATGTCTGCCATTGTGCGGTCGCCCGAGCACCAGCCCA GTGCCATGAGCCTGCTTGCCCCACCATCCAGCCGCAGAAAGGAGTCGTCAACTCCAGAGGAATTCAGCCG GCGTCTGAAAGAGCGCATGCACCACAACATCCCTCACCGGTTTAATGTGGGCCTGAACATGAGAGCCACC AAGTGCGCCGTGTGTCTGGATACTGTGCACTTTGGACGCCAGGCATCCAAATGCCTCGAATGTCAAGTGA TGTGTCACCCCAAATGCTCCACCTGCTTGCCCGCTACCTGTGGCCTGCCAGCTGAATATGCCACACACTT CACTGAGGCCTTCTGCCGGGACAAAATGAACTCCCCGGGGCTCCAGAGCAAGGAGCCTGGCAGCAGCTTG CACCTGGAAGGGTGGATGAAAGTGCCCAGAAATAACAAACGGGGACAGCAAGGCTGGGACAGGAAGTACA TTGTTCTGGAGGGGTCAAAAGTCCTCATCTATGACAATGAAGCCAGAGAAGCTGGACAGAGGCCGGTGGA AGAATTTGAGCTGTGCCTTCCTGACGGGGACGTATCTATTCATGGTGCCGTTGGTGCTTCAGAACTTGCA AATACCGCCAAAGCAGATGTCCCATACATCCTGAAGATGGAGTCTCACCCACACACCACCTGCTGGCCTG GGAGAACCCTCTACTTGCTAGCACCCAGCTTCCCCGACAAGCAGCGCTGGGTCACCGCCTTAGAATCTGT CGTCGCAGGTGGGAGAGTTTCTAGGGAAAAGGCCGAAGCCGATGCTAAATTACTTGGAAACTCTCTGCTG AAACTGGAGGGCGATGACCGGCTTGACATGAACTGCACCCTGCCCTTCAGTGACCAGGTAGTGCTGGTGG GCACGGAGGAAGGCCTCTACGCCCTGAATGTCTTGAAAAACTCCTTAACCCACATCCCAGGGATTGGCGC AGTCTTCCAAATTTACATCATCAAGGACCTGGAGAAGCTGCTCATGATAGCAGGGGAAGAGCGGGCTCTG TGCCTGGTGGACGTGAAGAAGGTGAAGCAGTCCCTGGCGCAGTCACACCTGCCTGCCCAGCCCGACGTCT CCCCCAACATATTCGAAGCCGTCAAAGGCTGCCACTTGTTCGCTGCTGGCAAGATCGAGAACAGCCTGTG CATCTGCGCCGCTATGCCAAGCAAAGTCGTCATCCTCCGCTACAATGACAACCTCAGCAAGTACTGCATC CGCAAGGAGATCGAGACCTCAGAGCCCTGCAGCTGTATCCACTTCACCAACTACAGCATCCTCATTGGGA CCAACAAATTCTATGAGATCGACATGAAGCAGTACACGCTTGACGAGTTCCTGGACAAGAACGACCATTC CTTGGCACCTGCTGTGTTCGCCTCCTCGTCCAACAGCTTCCCTGTCTCCATTGTGCAGGCGAACAGCGCC GGGCAGCGAGAAGAATACCTGCTGTGCTTCCACGAATTTGGGGTGTTCGTGGATTCTTACGGAAGACGTA GCCGCACAGATGATCTCAAGTGGAGTCGCTTACCTCTGGCCTTCGCCTACAGAGAACCTTATCTGTTTGT GACTCACTTCAACTCCCTGGAAGTCATTGAGATCCAGGCCAGATCCTCACTGGGGAGCCCTGCCCGAGCA TATCTGGAAATTCCAAACCCTCGCTACCTGGGCCCCGCGATTTCCTCCGGAGCGATTTACCTGGCCTCCT CATACCAGGACAAGTTAAGGGTCATATGCTGCAAAGGAAACCTCGTGAAGGAGTCAGGCACTGAGCAGCA CCGGGTGCCCTCCACCTCCCGCAGCAGCCCCAACAAGCGAGGCCCACCAACATACAACGAGCACATCACC AAACGCGTGGCCTCCAGCCCGGCGCCACCGGAAGGCCCCAGCCACCCCCGAGAGCCAAGCACACCGCACC GCTACCGAGACAGAGAGGGTCGGACAGAGCTGCGCAGGGACAAGTCTCCAGGCCGCCCTCTGGAGCGGGA GAAGTCCCCAGGCCGAATGCTCAGCACTAGGAGAGAGCGGTCCCCAGGGAGACTGTTTGAAGACAGCAGC AGGGGCCGGCTGCCTGCAGGAGCAGTGAGGACCCCACTGTCCCAGGTTAACAAGGTCTGGGACCAGTCTT CAGTA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR216404 representing NM_007708 Red=Cloning site Green=Tags(s) MLKFKYGVRNPPEASASEPIASRASRLNLFFQGKPPLMTQQQMSALSREGMLDALFALFEECSQPALMKM KHVSSFVQKYSDTIAELRELQPSARDFEVRSLVGCGHFAEVQVVREKATGDVYAMKIMKKKALLAQEQVS FFEEERNILSRSTSPWIPQLQYAFQDKNNLYLVMEYQPGGDLLSLLNRYEDQLDESMIQFYLAELILAVH SVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKMNSNKVDAKLPIGTPDYMAPEVLTVMNEDRRGTYG LDCDWWSVGVVAYEMVYGKTPFTEGTSARTFNNIMNFQRFLKFPDDPKVSSELLDLLQSLLCVQKERLKF EGLCCHPFFARTDWNNIRNSPPPFVPTLKSDDDTSNFDEPEKNSWVSSSVCQLSPSGFSGEELPFVGFSY SKALGYLGRSESVVSSLDSPAKVSSMEKKLLIKSKELQDSQDKCHKMEQEMTRLHRRVSEVEAVLSQKEV ELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLHDIREQSRKLQEIKEQEYQAQV EEMRLMMNQLEEDLVSARRRSDLYESELRESRLAAEEFKRKANECQHKLMKAKDQGKPEVGEYSKLEKIN AEQQLKIQELQEKLEKAVKASTEATELLQNIRQAKERAERELEKLHNREDSSEGIKKKLVEAEERRHSLE NKVKRLETMERRENRLKDDIQTKSEQIQQMADKILELEEKHREAQVSAQHLEVHLKQKEQHYEEKIKVLD NQIKKDLADKESLENMMQRHEEEAHEKGKILSEQKAMINAMDSKIRSLEQRIVELSEANKLAANSSLFTQ RNMKAQEEMISELRQQKFYLETQAGKLEAQNRKLEEQLEKISHQDHSDKSRLLELETRLREVSLEHEEQK LELKRQLTELQLSLQERESQLTALQAARAALESQLRQAKTELEETTAEAEEEIQALTAHRDEIQRKFDAL RNSCTVITDLEEQLNQLTEDNAELNNQNFYLSKQLDEASGANDEIVQLRSEVDHLRREITEREMQLTSQK QTMEALKTTCTMLEEQVLDLEALNDELLEKERQWEAWRSVLGDEKSQFECRVRELQRMLDTEKQSRARAD QRITESRQVVELAVKEHKAEILALQQALKEQKLKAESLSDKLNDLEKKHAMLEMNARSLQQKLETERELK QRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENIQVLYSHEKVKMEGTISQQ TKLIDFLQAKMDQPAKKKKVPLQYNELKLALEKEKARCAELEEALQKTRIELRSAREEAAHRKATDHPHP STPATARQQIAMSAIVRSPEHQPSAMSLLAPPSSRRKESSTPEEFSRRLKERMHHNIPHRFNVGLNMRAT KCAVCLDTVHFGRQASKCLECQVMCHPKCSTCLPATCGLPAEYATHFTEAFCRDKMNSPGLQSKEPGSSL HLEGWMKVPRNNKRGQQGWDRKYIVLEGSKVLIYDNEAREAGQRPVEEFELCLPDGDVSIHGAVGASELA NTAKADVPYILKMESHPHTTCWPGRTLYLLAPSFPDKQRWVTALESVVAGGRVSREKAEADAKLLGNSLL KLEGDDRLDMNCTLPFSDQVVLVGTEEGLYALNVLKNSLTHIPGIGAVFQIYIIKDLEKLLMIAGEERAL CLVDVKKVKQSLAQSHLPAQPDVSPNIFEAVKGCHLFAAGKIENSLCICAAMPSKVVILRYNDNLSKYCI RKEIETSEPCSCIHFTNYSILIGTNKFYEIDMKQYTLDEFLDKNDHSLAPAVFASSSNSFPVSIVQANSA GQREEYLLCFHEFGVFVDSYGRRSRTDDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQARSSLGSPARA YLEIPNPRYLGPAISSGAIYLASSYQDKLRVICCKGNLVKESGTEQHRVPSTSRSSPNKRGPPTYNEHIT KRVASSPAPPEGPSHPREPSTPHRYRDREGRTELRRDKSPGRPLEREKSPGRMLSTRRERSPGRLFEDSS RGRLPAGAVRTPLSQVNKVWDQSSV SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_007708
ORF Size	6165 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_007708.3, NP_031734.3
RefSeq Size	6898 bp
RefSeq ORF	6168 bp
Locus ID	12704
MW	235.8 kDa
Gene Summary	Plays a role in cytokinesis. Required for KIF14 localization to the central spindle and midbody. Probable RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. Displays serine/threonine protein kinase activity. Plays an important role in the regulation of cytokinesis and the development of the central nervous system. Phosphorylates MYL9/MLC2.[UniProtKB/Swiss-Prot Function]

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