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Chpf (NM_001001566) Mouse Tagged ORF Clone
CAT＃: MR216423
- TrueORF®
Chpf (Myc-DDK-tagged) - Mouse chondroitin polymerizing factor (Chpf), transcript variant 1
ORF Plasmid: tGFP
"NM_001001566" in other vectors (5)
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CNY 5,672.00
CNY 6,750.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1700028N03Rik; AI414328; D1Bwg1363e |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR216423 representing NM_001001566
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGCGTCGCTGCTGCTGTCCGTGCTGCGGCCCGCGGGGCCCGTGGCCGTGGGCATCTCTCTGGGCT TCACCCTGAGCCTGCTCAGCGTCACCTGGGTGGAGGAGCCTTGCGGACCCGGGCCGCCCCAACCCGGAGA CTCTGAGCTGCCGCCGCGCGGCAACACCAACGCGGCGCGCCGGCCCAACTCGGTGCAGCCCGGATCCGAG CGCGAGAGGCCCGGGGCCGGCGCAGGCACCGGTGAGAGCTGGGAGCCTCGTGTCTTGCCCTACCATCCCG CGCAGCCAGGCCAGGCCACCAAGAAGGCCGTCAGAACTCGGTATATCAGCACGGAGCTGGGCATCAGGCA GAAGCTTCTGGTGGCAGTGCTGACCTCACAAGCCACGTTGCCTACACTGGGTGTGGCTGTAAACCGAACT CTGGGACACCGACTGGAGCATGTAGTGTTCCTGACCGGTGCGAGGGGCCGCCGGACACCTTCAGGCATGG CGGTGGTGGCACTGGGCGAAGAGAGGCCCATCGGACACCTGCACCTGGCGCTGCGCCACCTGCTGGAGCA ACACGGCGATGACTTTGACTGGTTTTTCCTAGTGCCTGATGCCACCTATACTGAAGCGCATGGACTGGAC CGCCTAGCTGGCCACCTCAGCCTTGCTTCAGCAACCCATCTCTATCTTGGCCGGCCGCAGGACTTCATCG GTGGAGATACTACCCCAGGCCGCTACTGCCACGGGGGCTTTGGAGTCTTGCTCTCTCGCACACTGCTACA GCAACTGCGCCCCCACCTGGAAAGCTGCCGCAACGACATCGTCAGTGCTCGCCCGGATGAGTGGTTGGGC CGCTGCATCCTTGATGCCACAGGCGTGGGCTGTACTGGTGACCACGAGGGAATGCACTACAACTACCTGG AACTGAGCCCCGGGGAGCCTGTGCAGGAGGGGGACCCTCGTTTCCGCAGCGCCTTGACAGCCCATCCCGT GCGTGACCCTGTGCACATGTACCAGCTGCACAAAGCTTTTGCCCGCGCTGAGCTGGACCGCACGTACCAG GAGATTCAAGAATTGCAGTGGGAGATCCAGAATACCAGCCGACTGGCTGCTGATGGGGAGAGAGCCTCTG CCTGGCCAGTGGGCATCCCAGCACCGTCTCGCCCTGCCTCACGCTTTGAGGTTCTGCGCTGGGACTACTT CACAGAACAATACGCGTTCTCCTGCGCCGATGGCTCTCCCCGCTGCCCGTTGCGTGGGGCCGACCAGGCT GATGTGGCTGACGTCCTGGGGACAGCCTTAGAGGAGCTCAACCGCCGTTACCAGCCAGCGCTGCAGCTCC AGAAGCAACAGCTGGTGAACGGCTACCGGCGTTTTGATCCAGCCCGAGGCATGGAGTACACACTAGACCT GCAGCTGGAAGCGCTGACACCCCAGGGTGGCCGCTGGCCCCTCACCCGCAGGGTGCAGCTCCTTCGGCCC TTGAGCCGAGTGGAGATCTTGCCTGTACCCTATGTCACCGAGGCTTCTCGGCTCACTGTGCTACTGCCGC TGGCTGCAGCGGAACGAGACCTGGCTTCTGGCTTCTTAGAAGCCTTTGCCACTGCAGCCCTGGAACCTGG TGATGCAGCAGCCTTGACCCTGCTGCTGCTGTATGAGCCACGCCAGGCCCAGCGGGCAGCCCACTCAGAC GTCTTCGCACCTGTCAAGGCCCACGTGGCAGAGCTAGAGCGGCGTTTCCCTGGTGCCCGGGTGCCCTGGC TCAGTGTGCAGACAGCAGCGCCCTCTCCACTGCGTCTCATGGATCTGCTGTCCAAGAAGCACCCACTAGA CACTCTGTTCCTGCTGGCCGGGCCAGACACGGTACTCACACCTGATTTCCTGAACCGCTGCCGCATGCAT GCCATCTCTGGCTGGCAGGCCTTCTTCCCCATGCACTTCCAGGCCTTCCACCCTGCTGTGGCTCCACCTC AGGGCCCTGGGCCACCAGAGCTGGGCCGTGACACCGGTCACTTTGATCGCCAGGCTGCCAGTGAGGCATG CTTCTACAACTCCGACTATGTGGCGGCCCGTGGCCGGCTGGTGGCGGCCTCGGAGCAGGAGGAGGAGCTG CTGGAGAGCCTGGATGTGTACGAGTTGTTTCTGCGCTTCTCCAACTTGCACGTGCTGAGAGCAGTAGAGC CAGCCTTGCTGCAGCGCTACCGGGCCCAGCCGTGCAGTGCACGGCTCAGTGAAGACCTTTACCACCGCTG TCGCCAGAGCGTACTTGAGGGCCTTGGCTCCCGCACCCAGCTTGCCATGCTGCTCTTTGAGCAGGAACAG GGGAACAGCACC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR216423 representing NM_001001566
Red=Cloning site Green=Tags(s) MRASLLLSVLRPAGPVAVGISLGFTLSLLSVTWVEEPCGPGPPQPGDSELPPRGNTNAARRPNSVQPGSE RERPGAGAGTGESWEPRVLPYHPAQPGQATKKAVRTRYISTELGIRQKLLVAVLTSQATLPTLGVAVNRT LGHRLEHVVFLTGARGRRTPSGMAVVALGEERPIGHLHLALRHLLEQHGDDFDWFFLVPDATYTEAHGLD RLAGHLSLASATHLYLGRPQDFIGGDTTPGRYCHGGFGVLLSRTLLQQLRPHLESCRNDIVSARPDEWLG RCILDATGVGCTGDHEGMHYNYLELSPGEPVQEGDPRFRSALTAHPVRDPVHMYQLHKAFARAELDRTYQ EIQELQWEIQNTSRLAADGERASAWPVGIPAPSRPASRFEVLRWDYFTEQYAFSCADGSPRCPLRGADQA DVADVLGTALEELNRRYQPALQLQKQQLVNGYRRFDPARGMEYTLDLQLEALTPQGGRWPLTRRVQLLRP LSRVEILPVPYVTEASRLTVLLPLAAAERDLASGFLEAFATAALEPGDAAALTLLLLYEPRQAQRAAHSD VFAPVKAHVAELERRFPGARVPWLSVQTAAPSPLRLMDLLSKKHPLDTLFLLAGPDTVLTPDFLNRCRMH AISGWQAFFPMHFQAFHPAVAPPQGPGPPELGRDTGHFDRQAASEACFYNSDYVAARGRLVAASEQEEEL LESLDVYELFLRFSNLHVLRAVEPALLQRYRAQPCSARLSEDLYHRCRQSVLEGLGSRTQLAMLLFEQEQ GNST SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001001566 |
| ORF Size | 2322 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001001566.3, NP_001001566.1 |
| RefSeq Size | 3107 bp |
| RefSeq ORF | 2325 bp |
| Locus ID | 74241 |
| UniProt ID | Q6IQX7 |
| MW | 85.5 kDa |
| Gene Summary | Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer (By similarity). Isoform 2 may facilitate PRKN transport into the mitochondria. In collaboration with PRKN, isoform 2 may enhance cell viability and protect cells from oxidative stress (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221508 | Chpf (untagged) - Mouse chondroitin polymerizing factor (Chpf), transcript variant 1, (10ug) |
CNY 9,980.00 |
|
| MG210574 | Chpf (tGFP-tagged) - Mouse DNA segment, Chr 1, Brigham & Women's Genetics 1363 expressed (D1Bwg1363e), transcript variant 1 |
CNY 8,460.00 |
|
| MG216423 | Chpf (tGFP-tagged) - Mouse chondroitin polymerizing factor (Chpf) transcript variant 1, (10ug) |
CNY 7,410.00 |
|
| MR216423L3 | Lenti ORF clone of Chpf (Myc-DDK-tagged) - Mouse chondroitin polymerizing factor (Chpf), transcript variant 1 |
CNY 8,650.00 |
|
| MR216423L4 | Lenti ORF clone of Chpf (mGFP-tagged) - Mouse chondroitin polymerizing factor (Chpf), transcript variant 1 |
CNY 8,650.00 |
