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Med23 (NM_001166416) Mouse Tagged ORF Clone
CAT＃: MR216888
- TrueORF®
Med23 (Myc-DDK-tagged) - Mouse mediator complex subunit 23 (Med23), transcript variant 1
ORF Plasmid: tGFP
"NM_001166416" in other vectors (4)
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CNY 13,680.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 130kDa; 3000002A17Rik; Crsp3; ESTM7; mKIAA1216; Sur2; X83317 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR216888 representing NM_001166416
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTACCGATGGAGACGCAACTGCAGAGCATTTTCGAGGAGGTGGTGAAAACAGAAATTATAGAAGAGG CCTTCCCAGGAATGTTTATGGATACCCCCGAGGATGAAAAAACAAAACTAATTAGCTGCTTGGCGGCCTT CCGGCAGTTTTGGAGTGGACTTTCTCAGGAGTCTCATGAACAGTGTGTTCAGTGGATTGTTAAATTTATT CATGGCCAACACAGCCCCAAAAGAATTTCTTTCCTCTATGACTGCTTAGCAATGGCAGTTGAGACTGGTC TCCTTCCACCCAGGATGGTTTGTGAATCTTTGATAAACTCTGACTCTCTTGAATGGGAAAGAACACAGCT TTGGGCCTTAACATTTAAGCTGGTTCGGAAAATCATTGGAGGAGTGGATTACAAGGGTGTTCGAGACCTT CTCAAAGCGATTTTGGAGAAAATCTTGACAATCCCAAATACAGTGAGCTCTGCTGTTGTCCAGCAGCTTC TGGCAGCAAGAGAGGTTATAGCTTATATCTTAGAACGAAATGCCTGCCTATTGCCGGCCTACTTTGCAGT CACAGAGATCAGAAAACTATATCCTGAGGGAAAACTTCCACACTGGTTACTAGGAAACTTGGTATCAGAC TTTGTGGATACCTTCAGGCCCACAGCAAGGATTAACTCCATTTGTGGCCGATGCAGTCTTCTGCCAGTTG TGAACAACTCGGGAGCCATCTGTAACTCATGGAAACTGGACCCTGCAACTCTGCGATTTCCTTTGAAAGG CCTTTTGCCATATGATAAGGATCTGTTTGAACCGCAGACTGCTTTGTTGAGATATGTACTGGAGCAACCT TATTCCAGGGATATGGTCTGCAATATGCTAGGTTTAAATAAGCAGACCTTGAACATTGCTCAGCACAAGC AGCGCTGCCCTGTGCTGGAGGACCAGTTGGTGGACCTGGTGGTGTACGCCATGGAGAGGTCAGAGACCGA GGAGAAGTTTGACGATGGGGGAACCAGCCAGCTCCTGTGGCAGCACCTTTCCAGCCAGCTCATTTTCTTT GTGCTTTTCCAGTTTGCCAGTTTCCCACATATGGTCCTCTCTCTCCACCAGAAGTTAGCAGGGCGAGGAC TGATTAAAGGGCGAGACCATCTGATGTGGGTGCTGCTGCAGTTCATTTCTGGAAGTATTCAGAAAAATGC CCTCGCTGACTTCCTCCCTGTCATGAAGCTCTTTGACCTGCTGTACCCGGAGAAGGAGTGTATCCCAGTT CCTGATATTAACAAACCCCAGTCAACGCACGCCTTTGCAATGACTTGTATTTGGATTCATCTTAATAGAA AAGCTCAAAATGGCGACTCCACGCTCCAGATCCCAATACCCCATTCCCTGAAGCTGCACCATGAGTTCCT CCAGCAGAGTCTGAGAAATAAAAGCTTACAGATGAATGACTATAAGATTGCCCTGCTATGTAACGCGTAT TCCACAAACTCAGAGTGTTTTACGTTACCTATGGGAGCTCTGGTAGAAACTATTTATGGAAATGGAATTA TGAGAGTGCCTCTCCCTGGAACGAGCTGTTTGGCCTCAGCATCAGTGACTCCCTTACCGATGAACCTCCT GGATTCACTGACGGTGCATGCCAAGATGAGCCTTATTCACAGCATTGCAACCAGAGTGATAAAACTGGCT CATACGAAATCCAGTGTTGCGCTGGCTCCAGCCCTAGTGGAAACTTACAGCCGTTTACTGGTCTACATGG AAATTGAGTCTTTGGGCATCAAAGGATTTATCAGTCAGCTCCTGCCCACTGTCTTCAAGTCCCACGCCTG GGGCATCCTGCACACACTGCTGGAGATGTTCAGCCACCGAATGCACCACATTCAGCCCCACTACCGAGTT CAGCTCCTGAGCCATCTCCACACACTGGCTGCAGTCGCACAGACCAACCAGAACCAGCTCCATCTGTGTG TGGAGAGCACTGCACTGAGGCTCATCACAGCCCTGGGAAGCTCAGAGGTCCAGCCGCAGTTCACGCGTTT CCTCAATGATCCCAAAACAGTGCTGTCCGCCGAATCTGAAGAGCTGAACCGAGCCTTGATCCTGACCTTA GCCAGAGCAACCCACGTCACAGATTTTTTTACAGGATCTGATTCAATACAGGGAACTTGGTGTAAAGATA TCCTTCAGACCATCATGAACTTTACTCCTCATAACTGGGCTTCACATACCCTTAGCTGTTTTCCAGCCCC TCTGCAGGCTTTCTTCAAGCAAAATAACGTGCCCCAGGAAAGCCGTTTTAATCTGAAGAAGAATGTGGAA GAAGAGTACAGGAAGTGGAAGTCAATGACTGATGAAAACGAGATCATCACCCAGTTCTCTGTGCAGGGCT TCCCTCCACTCTTCCTCTGTCTCCTCTGGAAGATGCTCCTGGAGACGGATCATATTAGTCAGATCGGCTA CAAGGTCCTGGAGAGGATTGGGGCCAGGGCCCTGGTGGCCCATGTGAGAACATTTGCAGATTTCCTGGTG TATGAGTTCTCTACGTCAGCAGGAGGTCAGCAACTCAATAAGTGCATTGAAATTCTCAACGACATGGTGT GGAAGTACAACATTGTCACGCTGGACAGACTCATTCTCTGCCTGGCTATGCGTAGTCATGAAGGAAATGA AGCCCAGGTTTGTTATTTCATAATTCAGTTGCTGTTGCTCAAACCAAATGACTTCAGAAACCGAGTAAGT GACTTTGTGAAGGAGAATTCCCCAGAGCACTGGCTCCAGAGTGACTGGCACACCAAGCACATGAGCTACC ACAAGAAATATCCCGAGAAGCTGTATTTTGAAGGCCTGGCAGAGCAGGTTGACCCTCCTGTACCAATCCA GTCCCCTTATCTGCCCATCTACTTTGGAAATGTGTGTCTCCGGTTCCTTCCAGTATTTGATATAGTAATC CACAGGTTTTTAGAGTTGCTTCCAGTGTCCAAATCACTAGAGACTCTCCTGGATCACCTAGGGGGCTTAT ATAAATTCCATGACCGGCCAGTGACTTATCTGTATAATACTTTGCACTATTATGAAATGTGCCTGAGAAA CCGTGACCATCTCAAACGAAAACTTGTCCATGCAATCATTGGCTCCCTCAAAGATAACCGGCCACAGGGC TGGTGTCTCAGTGATACCTACCTGAAGCATGCTATGAATGCACGGGAGGACAACCCCTGGGTTCCGGAGG ACTCCTACTACTGCAAGCTGATTGGCCGACTGGTAGACACCATGGCGGGCAAGTCTCCAGGACCCTTCCC AAACTGTGACTGGAGATTCAATGAGTTTCCCAACCCCGCTGCCCACGCTCTGCATGTGACGTGTGTGGAA CTCATGGCCTTGGCGGTTCCAGGCAAAGATGTGGGGAATGCCCTTCTCAATGTCGTCCTGAAAAGCCAGC CCTTAGTGCCAAGGGAGAACATTACAGCGTGGATGAATGCAATTGGTCTGATCATCACGGCCCTCCCAGA GCCGTACTGGATTGTGCTTCATGACCGAATTGTGAATGTGATCAGCAGTTCTAGCCTGACGTCAGAGACA GAGTGGGTCGGTTACCCGTTCCGCCTCTTCGACTTCACCGCTTGTCACCAGTCCTACTCGGAGATGAGCT GCAGCTATACGTTAGCTCTAGCCCATGCCGTGTGGCACCACTCCAGCATCGGGCAGCTCTCGCTTATCCC AAAGTTTCTCACTGAGGCGCTTCTCCCGGTGGTGAAGACTGAGTTCCAGTTGCTCTACGTGTACCATCTT GTGGGGCCATTTCTACAGAGGTTCCAACAGGAGAGAACCCGGTGCATGATTGAGATTGGCGTGGCATTTT ATGACATGCTGTTGAATGTAGACCAGTGCAGCACGCACTTAAATTACATGGACCCCATCTGCGACTTCCT GTATCATATGAAGTATATGTTTACTGGTGACAGTGTGAAAGAGCAAGTAGAGAAGATTATCTGTAATTTA AAGCCGGCTTTAAAGCTTCGTCTTCGATTCATCACGCACATTAGCAAGATGGAGCCGGCAGTGCCACCCC AGGCCCTCAACAGCGGGTCTCCAGCACCTCAGTCTAACCAGGTGCCAGCATCTCTGCCGGTGACTCAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR216888 representing NM_001166416
Red=Cloning site Green=Tags(s) MVPMETQLQSIFEEVVKTEIIEEAFPGMFMDTPEDEKTKLISCLAAFRQFWSGLSQESHEQCVQWIVKFI HGQHSPKRISFLYDCLAMAVETGLLPPRMVCESLINSDSLEWERTQLWALTFKLVRKIIGGVDYKGVRDL LKAILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSD FVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQP YSRDMVCNMLGLNKQTLNIAQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFF VLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPV PDINKPQSTHAFAMTCIWIHLNRKAQNGDSTLQIPIPHSLKLHHEFLQQSLRNKSLQMNDYKIALLCNAY STNSECFTLPMGALVETIYGNGIMRVPLPGTSCLASASVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLA HTKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSHRMHHIQPHYRV QLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTL ARATHVTDFFTGSDSIQGTWCKDILQTIMNFTPHNWASHTLSCFPAPLQAFFKQNNVPQESRFNLKKNVE EEYRKWKSMTDENEIITQFSVQGFPPLFLCLLWKMLLETDHISQIGYKVLERIGARALVAHVRTFADFLV YEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVS DFVKENSPEHWLQSDWHTKHMSYHKKYPEKLYFEGLAEQVDPPVPIQSPYLPIYFGNVCLRFLPVFDIVI HRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMCLRNRDHLKRKLVHAIIGSLKDNRPQG WCLSDTYLKHAMNAREDNPWVPEDSYYCKLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVE LMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVNVISSSSLTSET EWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEALLPVVKTEFQLLYVYHL VGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNL KPALKLRLRFITHISKMEPAVPPQALNSGSPAPQSNQVPASLPVTQ SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001166416 |
| ORF Size | 4128 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001166416.1, NP_001159888.1 |
| RefSeq Size | 4946 bp |
| RefSeq ORF | 4131 bp |
| Locus ID | 70208 |
| UniProt ID | Q80YQ2 |
| MW | 157.5 kDa |
| Gene Summary | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors (By similarity). Also required for transcriptional activation subsequent to the assembly of the pre-initiation complex. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224361 | Med23 (untagged) - Mouse mediator complex subunit 23 (Med23), transcript variant 1, (10ug) |
CNY 17,580.00 |
|
| MG216888 | Med23 (tGFP-tagged) - Mouse mediator complex subunit 23 (Med23) transcript variant 1, (10ug) |
CNY 15,010.00 |
|
| MR216888L3 | Lenti ORF clone of Med23 (Myc-DDK-tagged) - Mouse mediator complex subunit 23 (Med23), transcript variant 1 |
CNY 15,580.00 |
|
| MR216888L4 | Lenti ORF clone of Med23 (mGFP-tagged) - Mouse mediator complex subunit 23 (Med23), transcript variant 1 |
CNY 15,580.00 |
