Rmi2 (NM_001162932) Mouse Tagged ORF Clone

CAT#: MR216967

  • TrueORF®

Rmi2 (Myc-DDK-tagged) - Mouse RIKEN cDNA A630055G03 gene (A630055G03Rik)

ORF Plasmid: DDK tGFP



  "NM_001162932" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 1,200.00

CNY 3,705.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms A630055G03Rik; Gm537; Gm929
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR216967 representing NM_001162932
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTGCGGCGTCCGAGTCGCTGTCCTCCGGTGGCCCCGGGGCCGTGCGGCTGCCGCGCTTGCCGC
CACTCAAGGTACTGGCAGGACAGCTGCGGCGGCACGCAGAGGGTGGCCCGGGCGCGTGGCGGCTGTCGCG
GGCGGCGGTGGGCCGCGCTCCGCTGGAGCTGGTGGCCGTGTGGATGCAGGGTACCGTGCTGGCGGCGGAA
GGCGGCCAGGCCCGGCTGCGCGACTCGAGCGGAGCCTTCTCTGTGCGCGGCCTGGAGCGCGTACCACGCG
GCCGGCCCTGCCTCCTCCCAGGAAAGTATGTGATGGTGATGGGGGTGGTTCAGGCCTGCAGTCCTGAGCC
CTGCCTGCAGGCCGTGAAGATGACGGATCTTTCTGACAATCCTGTCCACGAAAGCATGTGGGAACTGGAG
GTGGAAGATTTACACAGGAATATTCCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR216967 representing NM_001162932
Red=Cloning site Green=Tags(s)

MAAAASESLSSGGPGAVRLPRLPPLKVLAGQLRRHAEGGPGAWRLSRAAVGRAPLELVAVWMQGTVLAAE
GGQARLRDSSGAFSVRGLERVPRGRPCLLPGKYVMVMGVVQACSPEPCLQAVKMTDLSDNPVHESMWELE
VEDLHRNIP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001162932
ORF Size 447 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001162932.1, NP_001156404.1
RefSeq Size 3739 bp
RefSeq ORF 450 bp
Locus ID 223970
UniProt ID Q3UPE3
MW 15.8 kDa
Gene Summary Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates. It is required to regulate sister chromatid segregation and to limit DNA crossover. Essential for the stability, localization, and function of BLM, TOP3A, and complexes containing BLM. In the RMI complex, it is required to target BLM to chromatin and stress-induced nuclear foci and mitotic phosphorylation of BLM.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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