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Aen (NM_001162939) Mouse Tagged ORF Clone
CAT＃: MR217018
- TrueORF®
Aen (Myc-DDK-tagged) - Mouse apoptosis enhancing nuclease (Aen), transcript variant 2
ORF Plasmid: tGFP
"NM_001162939" in other vectors (4)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2700083B06Rik; Isg20l1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR217018 representing NM_001162939
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGCAGGGGAAGTCCCCGAGTCTACTCAGTGTCCATCTCTCACCAGCCTGAATACCAAGGATGTGG TTCGGAGAAGACACAAGAGGAGAAGCCGGCAGCACCAGCGATTCATGGCCCGCAAGGCCTTGCTTCAGGA GCAGGAGCTGTTGAGCACGGCACCAGGGCCAGGTCTCTGCCTTCTGCCTTCACCATCACAGATGCCAGCG GTCACAGAGGCTTCGGACAGCAGGAGGCAACGTCCCAAGGCCAGGTCCGGCAGCAATGGCCTGTGCAGCA AAAAGTCTGTCCCCAGAGAAGCTCCAAGACCTGGGCCCATCAAGTGTGTGGCCATTGACTGTGAGATGGT GGGTACGGGCCCCCAAGGACGGGTGAGCGAGCTAGCCCGCTGCTCTGTGGTGAGCTACAGTGGTGACGTT CTCTATGACAAATACATCCGGCCTGAAATGCCCATTGTGGACTATCGCACCCGCTGGAGTGGCATCACCC GCCAGCACATGCACAAGGCTATCCCCTTTCAGGTGGCCCAGAAGGAGATCCTTAAGCTGTTGAAGGGCAA GGTGGTGGTGGGGCACGCACTGCACAATGACTTCCAGGCCCTGAAGTACGTCCACCCTCGGAGCCAGACC CGGGATACCACTTATGTCCCAAACCTGCTCAGCCAGCCCAGCTCCCTTATCCGAACTCGGGTCTCTCTTA AGGACCTGGCCTTGAACCTGCTGCACAAGAAGATCCAGGTCGGTCACCAGGGCCACTCATCTGTGGAAGA TGCCATGACAGCCATGGAGTTGTACCAGCTGGTGGAGGTGCAGTGGGAGCAGCAAGTGGCCAGCTCTGCC CAGGCCCCTGCTGAGGACAGGGGGCCCGACAGCAGCACTGACGTGGAGCAGTACATGGACGACCAGTACT GGCCCGAGGACCTGACCCAGAGCACCGGGGCCGAGACAAATGGAGGCCCAGATAGACAGGAGGGAGAGGA AGGGCAGGGAGCCAGGAGTGCTCCTCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217018 representing NM_001162939
Red=Cloning site Green=Tags(s) MVAGEVPESTQCPSLTSLNTKDVVRRRHKRRSRQHQRFMARKALLQEQELLSTAPGPGLCLLPSPSQMPA VTEASDSRRQRPKARSGSNGLCSKKSVPREAPRPGPIKCVAIDCEMVGTGPQGRVSELARCSVVSYSGDV LYDKYIRPEMPIVDYRTRWSGITRQHMHKAIPFQVAQKEILKLLKGKVVVGHALHNDFQALKYVHPRSQT RDTTYVPNLLSQPSSLIRTRVSLKDLALNLLHKKIQVGHQGHSSVEDAMTAMELYQLVEVQWEQQVASSA QAPAEDRGPDSSTDVEQYMDDQYWPEDLTQSTGAETNGGPDRQEGEEGQGARSAPP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001162939 |
| ORF Size | 1008 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 2466 bp |
| RefSeq ORF | 897 bp |
| Locus ID | 68048 |
| UniProt ID | Q9CZI9 |
| MW | 33.3 kDa |
| Gene Summary | Exonuclease with activity against single- and double-stranded DNA and RNA. Mediates p53-induced apoptosis. When induced by p53 following DNA damage, digests double-stranded DNA to form single-stranded DNA and amplifies DNA damage signals, leading to enhancement of apoptosis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC210680 | Aen (untagged) - Mouse apoptosis enhancing nuclease (Aen), transcript variant 2, (10ug) |
CNY 3,990.00 |
|
| MG217018 | Aen (tGFP-tagged) - Mouse apoptosis enhancing nuclease (Aen) transcript variant 2, (10ug) |
CNY 3,710.00 |
|
| MR217018L3 | Lenti ORF clone of Aen (Myc-DDK-tagged) - Mouse apoptosis enhancing nuclease (Aen), transcript variant 2 |
CNY 5,230.00 |
|
| MR217018L4 | Lenti ORF clone of Aen (mGFP-tagged) - Mouse apoptosis enhancing nuclease (Aen), transcript variant 2 |
CNY 6,424.00 |
