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Eml1 (NM_001043335) Mouse Tagged ORF Clone
CAT＃: MR217188
- TrueORF®
Eml1 (Myc-DDK-tagged) - Mouse echinoderm microtubule associated protein like 1 (Eml1), transcript variant 1
ORF Plasmid: tGFP
"NM_001043335" in other vectors (4)
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CNY 8,952.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1110008N23Rik; A930030P13Rik; AA171013; AI847476; AI853955; ELP79; EMAP; EMAPL; heco |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR217188 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACGGCTTCTCCAGCTATAGCAGCCTGTACGACACGTCCTCGCTGCTGCAGTTCTGCAACGATG ACAGCGCGTCTGCTGCCAGCAGCATGGAGGTGTCAGACCGCATCGCCTCTCTGGAGCAGCGCGTCCAGAT GCAGGAGGATGACATTCAACTGCTCAAGTCAGCGCTGGCCGACGTGGTTCGGAGGCTGAACATCACGGAG GAGCAACAGGCTGTGCTCAACAGGAAAGGACCTACCAAAGCGAGGCCACTGGGGCAGACCCTGCCATTGA GAACCACCGTCAACAATGGCACCGTGTTACCAAAGAAACCCAGTGCCTCCCTCCCGGCACCCTCCGGGGC CAGGAAAGAAGTAGTTGTGCCGGTAACCAAAAGCATCAATAGGACCAGCTCTTCCGAAAGAGTGTCTCCA GGTGGCCGGAGGGAGAGCTCTGGGGACTCCAAAGGAAGCCGGAACCGCACGGGCTCAACCAGTAGCTCCT CCAGCGGCAAGAAGAACAGTGAGAGCAAACCCAAGGAGCCCGCATTCAGTCCAGAAGAAGGATATGTAAA AATGTTTCTTCGAGGCCGTCCGGTCACCATGTACATGCCCAAAGACCAAGTGGATTCGTACAGTTTGGAA GCAAAAGCTGAGTTACCAACGAAGCGGCTGAAACTGGAGTGGGTCTACGGGTACCGGGGGCGAGACTGTC GCAATAACTTGTACTTGCTCCCGACCGGGGAGACCGTGTACTTCATTGCGTCTGTCGTGGTGCTTTACAA TGTGGAGGAGCAGCTGCAGAGGCATTACGCGGGCCACAACGATGACGTCAAGTGCCTAGCGGTCCATCCT GACAGGATCACCATAGCAACGGGACAAGTGGCGGGCACATCTAAGGATGGAAAGCAACTGCCACCACATG TGCGCATCTGGGACTCTGTGACACTGAACACTCTGCATGTCATTGGAATAGGCTTTTTTGACCGGGCTGT CACCTGCATCGCATTCTCAAAGTCTAACGGAGGAGGCCATCTCTGTGCTGTGGATGATTCCAACGATCAC GTGCTGTCCGTGTGGGATTGGCAGAAAGAAGAGAGACTGGCCGACGTGAAGTGTTCGAACGAAGCGGTAT TTGCGGCGGACTTCCACCCCACAGACACCAATATCATAGTCACCTGCGGGAAGTCACATCTCTACTTTTG GACCCTAGAAGGAAATTCCCTTAACAAGAAGCAAGGGTTGTTTGAGAAACAAGAGAAGCCAAAGTTTGTT CTCTGCGTGACGTTTTCTGAAAATGGCGACACCATTACTGGAGATTCAAGCGGCAACATCTTGGTGTGGG GGAAAGGTACAAATCGGATAAGCTATGCAGTTCAAGGGGCCCACGAGGGCGGCATTTTTGCACTTTGTAT GCTGAGAGACGGGACGCTGGTGTCCGGAGGAGGGAAGGACCGGCGACTCATTTCCTGGAACGGAAACTAT CAAAAACTTCACAAAGCAGAGATTCCTGAGCAGTTTGGCCCAATACGGACAGTAGCCGAAGGGAAGGGCA ACGTCATCTTGATAGGCACTACTAGAAACTTTGTCCTGCAAGGCACCTTGTCAGGGGACTTCACACCCAT CACTCAGGGCCACACCGATGAGCTCTGGGGGCTAGCCATCCATGCCTCCAAGCCTCAGTTCCTGACTTGT GGACATGACAAACATGCCACTCTCTGGGACGCTGTCGGTCACCGGCCAGTCTGGGACAAAATCATAGAGG ATCCAGCTCAGTCCTCTGGTTTTCATCCTTCCGGGTCTGTGGTTGCAGTGGGGACACTGACTGGGAGGTG GTTTGTGTTTGACACGGAGACAAAGGACTTGGTCACCGTCCACACGGATGGGAATGAGCAGCTGTCCGTG ATGCGGTATTCTCCAGATGGGAACTTCTTAGCAATCGGCTCCCATGACAACTGCATCTACATATATGGAG TTACCGACAATGGAAGGAAGTACACACGAGTTGGCAAGTGCTCCGGCCATTCCAGCTTCATCACCCACTT GGACTGGTCCGTGAACTCACAATTCCTGGTGTCAAATTCCGGGGACTACGAGATCCTCTACTGGGTTCCG TCTGCCTGTAAGCAAGTCGTGAGTGTGGAAACCACAAGGGACATCGAGTGGGCCACCTATACCTGCACCT TGGGATTCCACGTCTTTGGAGTGTGGCCGGAGGGCTCCGATGGGACAGACATCAACGCCGTCTGCCGGGC TCACGAGAGAAAGCTCTTGTGCACAGGCGATGACTTCGGCAAAGTGCACCTCTTCTCATACCCGTGCTCA CAGTTCCGGGCTCCAAGCCACATCTACAGTGGACACAGCAGCCACGTCACCAACGTGGACTTCCTCTGTG AAGACAGCCACCTTATCTCCACGGGTGGGAAAGACACAAGCATCATGCAGTGGCGAGTCATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217188 protein sequence
Red=Cloning site Green=Tags(s) MEDGFSSYSSLYDTSSLLQFCNDDSASAASSMEVSDRIASLEQRVQMQEDDIQLLKSALADVVRRLNITE EQQAVLNRKGPTKARPLGQTLPLRTTVNNGTVLPKKPSASLPAPSGARKEVVVPVTKSINRTSSSERVSP GGRRESSGDSKGSRNRTGSTSSSSSGKKNSESKPKEPAFSPEEGYVKMFLRGRPVTMYMPKDQVDSYSLE AKAELPTKRLKLEWVYGYRGRDCRNNLYLLPTGETVYFIASVVVLYNVEEQLQRHYAGHNDDVKCLAVHP DRITIATGQVAGTSKDGKQLPPHVRIWDSVTLNTLHVIGIGFFDRAVTCIAFSKSNGGGHLCAVDDSNDH VLSVWDWQKEERLADVKCSNEAVFAADFHPTDTNIIVTCGKSHLYFWTLEGNSLNKKQGLFEKQEKPKFV LCVTFSENGDTITGDSSGNILVWGKGTNRISYAVQGAHEGGIFALCMLRDGTLVSGGGKDRRLISWNGNY QKLHKAEIPEQFGPIRTVAEGKGNVILIGTTRNFVLQGTLSGDFTPITQGHTDELWGLAIHASKPQFLTC GHDKHATLWDAVGHRPVWDKIIEDPAQSSGFHPSGSVVAVGTLTGRWFVFDTETKDLVTVHTDGNEQLSV MRYSPDGNFLAIGSHDNCIYIYGVTDNGRKYTRVGKCSGHSSFITHLDWSVNSQFLVSNSGDYEILYWVP SACKQVVSVETTRDIEWATYTCTLGFHVFGVWPEGSDGTDINAVCRAHERKLLCTGDDFGKVHLFSYPCS QFRAPSHIYSGHSSHVTNVDFLCEDSHLISTGGKDTSIMQWRVI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001043335 |
| ORF Size | 2445 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001043335.1, NP_001036800.1 |
| RefSeq Size | 4160 bp |
| RefSeq ORF | 2445 bp |
| Locus ID | 68519 |
| UniProt ID | Q05BC3 |
| MW | 89.7 kDa |
| Gene Summary | Modulates the assembly and organization of the microtubule cytoskeleton, and probably plays a role in regulating the orientation of the mitotic spindle and the orientation of the plane of cell division. Required for normal proliferation of neuronal progenitor cells in the developing brain and for normal brain development. Does not affect neuron migration per se.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221810 | Eml1 (untagged) - Mouse echinoderm microtubule associated protein like 1 (Eml1), transcript variant 1, (10ug) |
CNY 10,450.00 |
|
| MG217188 | Eml1 (tGFP-tagged) - Mouse echinoderm microtubule associated protein like 1 (Eml1) transcript variant 1, (10ug) |
CNY 10,552.00 |
|
| MR217188L3 | Lenti ORF clone of Eml1 (Myc-DDK-tagged) - Mouse echinoderm microtubule associated protein like 1 (Eml1), transcript variant 1 |
CNY 10,070.00 |
|
| MR217188L4 | Lenti ORF clone of Eml1 (mGFP-tagged) - Mouse echinoderm microtubule associated protein like 1 (Eml1), transcript variant 1 |
CNY 10,070.00 |
