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Eif4enif1 (NM_023743) Mouse Tagged ORF Clone
CAT＃: MR217308
- TrueORF®
Eif4enif1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4E nuclear import factor 1 (Eif4enif1), transcript variant 1
ORF Plasmid: tGFP
"NM_023743" in other vectors (5)
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CNY 7,200.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2610509L04Rik; A930019J01Rik; AA410001; AU021239; Clast4; D11Ertd166e |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR217308 representing NM_023743
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAAAAGTGTGGCTGAAACAGAAAATGGAGATGCTTTCCTTGAGCTGAAGAAACTGCCCACCTCCA AATCCCCCCATCGCTATACGAAAGAGGAGCTCTTGGATATAAAAGAACGTCCATACTCCAAGCAGAGGCC ATCATGCCTCTCTGAAAAATATGACAGTGATGGTGTCTGGGACCCTGAGAAGTGGCATGCCTCTCTCTAC CCAGCCTCGGGGCGGAGCTCACCAGTGGAAAGTCTGAAGAAAGAGTCAGAATCCGATCGACCTTCTCTGG TGCGCAGAATAGCAGATCCACGAGAGCGAGTGAAAGAAGATGACTTAGATGTTGTTCTCAGCCCACAGAG ACGAAGCTTTGGAGGTGGCTGCCATGTGACAGCTGCTGTTAGCTCCCGACGATCTGGCAGTCCATTGGAG AAAGACAGTGATGGACTTCGCCTGCTTGGTGGACGAAGAATTGGCAGTGGAAGGATAATTTCTGCCCGGG CCTTTGAAAAGGATCACCGTCTCAGTGATAAGGACCTGCGAGACTTGAGAGACCGAGACAGAGAGAGGGA CTACAAGGACAAACGTTTCAGGAGGGAGTTTGGAGATAGTAAACGTGTCTTTGGTGAGCGTAGAAGAAAC GATTCATACACTGAGGAAGAACCAGAATGGTTTTCAGCTGGACCCACCAGTCAGTCTGAAACCATAGAGC TGACTGGCTTTGATGATAAGATTCTAGAGGAGGATCACAAAGGCAGAAAAAGAACAAGGCGACGGACTGC CTCTGTGAAGGAAGGCATAGTGGAGTGCAATGGAGGGGTGGCTGAAGAGGACGAGGTGGAAGTCATTCTG GCCCAGGAGCCCTCTGCTGATCAGGAAGTTCCAAGGGATGTCATCCTACCTGAGCAGTCCCCAGGAGAAT TTGACTTTAATGAATTCTTTAACCTTGATAAGGTGCCATGCTTGGCTTCGATGATAGAAGATGTTCTGGG GGAAGGATCGGTCTCTGCCAGCCGCTTCAGTAGGTGGTTCTCTAACCCAAGCCGGTCAGGGAGCAGATCC AGCAGCCTTGGATCCACACCCCATGAAGAACTTGAGAGACTGGCAGGTTTGGAGCAAGCCGTCCTCTCTC CTGGACAGAACTCGGGGAATTATTTTGCTCCTATACCATCGGAAGACCATGCTGAAAATAAAGTGGACAT TTTAGAAATGCTACAGAAAGCCAAAGTAGATTTAAAACCTCTTCTTTCTAGCCTTTCTGCAAATAAAGAA AAACTTAAGGAGAGCTCACATTCAGGGGTTGTACTTTCAGTGGAAGAGGTAGAAGCAGGGCTGAAGGGCT TGAAAGTGGACCAGCAAATGAAGAATTCAACTCCCTTCATGGCAGAACATTTAGAGGAGACCCTGAGTGC TGCGAGTAGCAATAGACAGCTCAAGAAAGATGGAGATATGACTGCATTCAACAAGCTAGTGAACACCATG AAGGCAAGTGGAACTTTGCCTACCCAGCCCAAAGTCAGTCGAAACGTTGAAAGTCACTTATTGGCCCCTG CTGAGATTCCAGGCCAGCCTGTTTCTAAAAACATCCTACAGGAACTTCTGGGTCAACCGGTTCAGAGACC CGCTTCTTCCAATCTTCTCAGTGGCCTTATGGGGAGCTTGGAGGCTACTGCATCTTTATTGAGCCAAAGA GCGCCCTCTCCTCCAATGTCACAGGTATTTCGAACTCAAGCAGCCTCAGCAGACTACCTTCATCCAAGGA TACCATCACCAATAGGTTTCCCTTCAGGACCCCAGCAACTACTTGGAGATCCATTCCAAGGCATGCGCAA GCCCATGAGTCCTGTCTCAGCCCAGATGAGCCAGCTAGAATTGCAGCAAGCTGCTTTGGAGGGGCTGGCC CTACCACATGACCTTGCAGTCCAGACAGCACCCTTCTACCAGCCTGGTTTTAGCAAACCACAAGTGGACA GAACCAGAGATGGGCTCAGAAACAGGCAACAGCGAATGTCAAAGTCACCAGCACCCATGCATGGTGGAAA TTCCTCTTCCCCAGCTCCTGCTGCCTCCATCACAAGCATGCTTTCTCCTTCCTTCACCCCTACCTCAGTG ATCCGGAAGATGTATGAAAGCAGAGAGAAAACCAAGGAGGAAATGGCACCTGGAATGGTGGTTCCTGGTG ATGGTAAAGAGGATACTCAGAAGACCAGCGAAGAGAACCTGCTGTCATCCAATCCCATACCCAATACTGA TCAAGACTCTTCTACGACAAATCCTAAGCTTTCAACATTACAGCGGTCTTCATGTTCCACCCCACTCTCC CAGACCAGCCGTTATACCAAAGAACAAGATTATCGACCTAAAACAGCAGGAAGGAAAACACCTACCTTGG CATCCCCAGTCCCAGGAACACCTTTTCTCCGCCCTACCCACCAAGTTCCCCTTGTCCCTCATGTTCCTAT TGTTCGACCTGCACACCAGCTTCACCCAGGATTAGTCCAAAGGTTGATAGCCCAAGGGGTACATCCACAG CATCTTCCAAGTTTGCTCCAAGCTGGTGTGCTTCCTCCTGGGATAGACATGGCTCCTTTACAGGGGCTAT CTGGCCCACTCCTGGGTCAACCCTTGTACCCTTTGGTCTCTGCTGCTAGTCACCCTCTCCTAAACCCTCG TCCTGGGACCCCTCTGCATTTGGCGGTGATGCAGCAGCAGCTACAGCGCTCAGTTCTGCATCCTCCAGGC TCTAGTTCCCAGGCAGCAGCTATCAGCGTTCAGACTCCTCAGAATGTACCCAGCCGGTCGGGCATGCCCC ACATGCACTCCCAGCTGGAGCATCGTACCAGCCAAAGGAGCAGCTCCCCTGTGGGCCTTGCCAAATGGTT TGGCTCAGATGTGCTACAGCAGCCTCTGCCCTCCATGCCCACCAAAGTCATCAGTGTAGATGAACTGGAA TATAGACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217308 representing NM_023743
Red=Cloning site Green=Tags(s) MEKSVAETENGDAFLELKKLPTSKSPHRYTKEELLDIKERPYSKQRPSCLSEKYDSDGVWDPEKWHASLY PASGRSSPVESLKKESESDRPSLVRRIADPRERVKEDDLDVVLSPQRRSFGGGCHVTAAVSSRRSGSPLE KDSDGLRLLGGRRIGSGRIISARAFEKDHRLSDKDLRDLRDRDRERDYKDKRFRREFGDSKRVFGERRRN DSYTEEEPEWFSAGPTSQSETIELTGFDDKILEEDHKGRKRTRRRTASVKEGIVECNGGVAEEDEVEVIL AQEPSADQEVPRDVILPEQSPGEFDFNEFFNLDKVPCLASMIEDVLGEGSVSASRFSRWFSNPSRSGSRS SSLGSTPHEELERLAGLEQAVLSPGQNSGNYFAPIPSEDHAENKVDILEMLQKAKVDLKPLLSSLSANKE KLKESSHSGVVLSVEEVEAGLKGLKVDQQMKNSTPFMAEHLEETLSAASSNRQLKKDGDMTAFNKLVNTM KASGTLPTQPKVSRNVESHLLAPAEIPGQPVSKNILQELLGQPVQRPASSNLLSGLMGSLEATASLLSQR APSPPMSQVFRTQAASADYLHPRIPSPIGFPSGPQQLLGDPFQGMRKPMSPVSAQMSQLELQQAALEGLA LPHDLAVQTAPFYQPGFSKPQVDRTRDGLRNRQQRMSKSPAPMHGGNSSSPAPAASITSMLSPSFTPTSV IRKMYESREKTKEEMAPGMVVPGDGKEDTQKTSEENLLSSNPIPNTDQDSSTTNPKLSTLQRSSCSTPLS QTSRYTKEQDYRPKTAGRKTPTLASPVPGTPFLRPTHQVPLVPHVPIVRPAHQLHPGLVQRLIAQGVHPQ HLPSLLQAGVLPPGIDMAPLQGLSGPLLGQPLYPLVSAASHPLLNPRPGTPLHLAVMQQQLQRSVLHPPG SSSQAAAISVQTPQNVPSRSGMPHMHSQLEHRTSQRSSSPVGLAKWFGSDVLQQPLPSMPTKVISVDELE YRQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_023743 |
| ORF Size | 2949 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_023743.2, NP_076232.2 |
| RefSeq Size | 3709 bp |
| RefSeq ORF | 2952 bp |
| Locus ID | 74203 |
| UniProt ID | Q9EST3 |
| MW | 108 kDa |
| Gene Summary | Nucleoplasmic shuttling protein, which inhibits translation initiation. Mediates the nuclear import of EIF4E by a piggy-back mechanism.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201951 | Eif4enif1 (untagged) - Mouse eukaryotic translation initiation factor 4E nuclear import factor 1 (Eif4enif1), transcript variant 1, (10ug) |
CNY 7,208.00 |
|
| MG211382 | Eif4enif1 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 4E nuclear import factor 1 (Eif4enif1) |
CNY 7,890.00 |
|
| MG217308 | Eif4enif1 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 4E nuclear import factor 1 (Eif4enif1) transcript variant 1, (10ug) |
CNY 7,890.00 |
|
| MR217308L3 | Lenti ORF clone of Eif4enif1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4E nuclear import factor 1 (Eif4enif1), transcript variant 1 |
CNY 9,030.00 |
|
| MR217308L4 | Lenti ORF clone of Eif4enif1 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 4E nuclear import factor 1 (Eif4enif1), transcript variant 1 |
CNY 9,030.00 |
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