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Pold1 (NM_011131) Mouse Tagged ORF Clone
CAT＃: MR217545
- TrueORF®
Pold1 (Myc-DDK-tagged) - Mouse polymerase (DNA directed), delta 1, catalytic subunit (Pold1)
ORF Plasmid: tGFP
"NM_011131" in other vectors (4)
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CNY 11,020.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 125kDa |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR217545 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTGTAAGCGGCGACAAGGACCAGGCCCTGGGGTGCCCCCAAAGCGGGCTCGAGGGCACCTCTGGG ATGAGGACGAGCCTTCGCCGTCGCAGTTTGAGGCGAACCTGGCACTGCTGGAGGAAATAGAGGCTGAGAA CCGGCTGCAGGAGGCAGAGGAGGAGCTGCAGCTGCCCCCAGAGGGCACCGTGGGTGGGCAGTTTTCCACT GCAGACATTGACCCTCGGTGGCGGCGGCCCACCCTACGTGCCCTGGACCCCAGCACGGAGCCCCTCATCT TCCAGCAGCTGGAGATTGACCACTATGTGGGCTCAGCACCACCCCTGCCAGAAGGGCCCCCGCCATCCCG GAACTCAGTGCCCATACTGAGGGCCTTTGGGGTCACCGATGAAGGCTTCTCCGTCTGCTGCCACATACAG GGCTTTGCCCCCTACTTCTACACCCCCGCGCCTCCTGGTTTTGGGGCCGAGCACCTGAGTGAGCTGCAGC AGGAGCTGAACGCAGCCATCAGCCGGGACCAGCGCGGTGGGAAGGAGCTCTCAGGGCCGGCAGTGCTGGC AATAGAGCTATGCTCCCGTGAGAGCATGTTTGGGTACCACGGTCATGGCCCTTCTCCATTTCTCCGCATC ACCCTGGCACTACCCCGCCTTATGGCACCAGCCCGCCGCCTTCTGGAACAGGGTGTCCGAGTGCCAGGCC TGGGCACCCCGAGCTTCGCACCCTACGAAGCCAACGTGGACTTTGAGATCCGGTTCATGGTGGATGCTGA CATTGTGGGATGCAACTGGTTGGAGCTGCCAGCTGGAAAGTACGTTCGGAGGGCGGAGAAGAAGGCCACC CTGTGTCAGCTGGAGGTGGACGTGCTGTGGTCAGATGTGATCAGTCACCCACCGGAGGGGCAGTGGCAGC GCATTGCACCCCTGCGTGTGCTTAGCTTCGACATCGAGTGTGCTGGCCGAAAAGGCATCTTCCCTGAGCC TGAGCGTGACCCCGTGATCCAGATCTGTTCTCTGGGGCTGCGCTGGGGGGAGCCGGAGCCATTCTTGCGT CTGGCACTCACGCTGCGGCCCTGTGCCCCCATCCTGGGTGCCAAAGTGCAGAGCTATGAGCGGGAAGAAG ACCTGCTCCAGGCCTGGGCCGACTTCATCCTTGCCATGGACCCTGACGTGATCACCGGCTACAACATTCA GAACTTTGACCTCCCATACCTCATCTCTCGGGCACAGGCCCTAAAGGTGGACCGCTTCCCTTTCCTGGGC CGCGTGACTGGTCTCCGCTCCAACATCCGTGACTCCTCCTTCCAATCAAGGCAGGTCGGCCGGCGGGACA GTAAGGTGATCAGCATGGTGGGTCGCGTTCAGATGGATATGCTGCAGGTGCTGCTTCGGGAACACAAGCT CCGCTCCTACACGCTCAACGCTGTGAGTTTCCACTTCCTGGGCGAGCAGAAGGAGGACGTTCAGCACAGC ATCATCACCGACCTGCAGAATGGGAACGAACAGACGCGCCGCCGCCTGGCCGTGTACTGCCTGAAGGACG CCTTTCTGCCACTCCGACTACTAGAGCGCCTTATGGTGCTGGTGAACAATGTGGAGATGGCGCGTGTCAC CGGTGTACCCCTTGGGTACCTGCTCACCCGGGGCCAGCAGGTCAAGGTCGTGTCTCAGCTGCTGCGCCAG GCCATGCGCCAGGGGCTGCTGATGCCTGTGGTGAAGACCGAGGGCGGTGAGGACTACACGGGAGCCACAG TCATTGAGCCCCTCAAAGGGTACTATGACGTCCCCATTGCCACCCTGGACTTCTCCTCCTTGTACCCATC CATCATGATGGCCCATAATCTGTGCTACACCACGCTGCTCCGACCTGGGGCTGCCCAGAAGCTGGGCCTT AAACCAGATGAGTTCATCAAGACACCCACTGGGGATGAGTTTGTGAAGTCATCTGTACGGAAGGGCCTCC TGCCCCAGATCCTGGAGAATCTGCTGAGTGCCCGCAAGAGGGCCAAGGCTGAGCTGGCTCAGGAGACGGA CCCCCTGCGGCGACAGGTCTTGGACGGCCGGCAACTGGCACTAAAAGTGAGTGCCAACTCCGTATATGGC TTCACTGGTGCCCAGGTGGGCAAGCTGCCATGTTTGGAGATCTCCCAGAGTGTCACTGGGTTCGGGCGGC AGATGATTGAGAAAACCAAGCAGCTTGTGGAGTCCAAGTACACCGTGGAAAATGGCTACGATGCCAACGC CAAGGTAGTCTACGGTGACACGGACTCTGTGATGTGCCGGTTTGGCGTCTCCTCTGTGGCTGAAGCAATG TCTCTGGGGCGGGAGGCTGCAAACTGGGTATCCAGTCACTTCCCATCACCCATCCGGCTGGAGTTCGAGA AGGTTTACTTCCCATACCTGCTCATCAGCAAGAAGCGCTATGCTGGCCTGCTCTTCTCCTCCCGCTCTGA TGCCCATGACAAAATGGACTGCAAGGGCCTGGAGGCTGTGCGCAGGGACAACTGTCCCCTGGTGGCCAAC CTCGTTACATCCTCCCTGCGCCGGATCCTCGTGGACCGGGACCCTGATGGGGCAGTAGCCCATGCCAAGG ACGTCATCTCGGACCTGCTGTGCAACCGCATAGACATCTCCCAGCTGGTCATCACCAAAGAGTTGACCCG AGCAGCAGCAGACTATGCTGGCAAGCAGGCTCACGTGGAGCTGGCTGAGAGGATGAGGAAGCGCGACCCC GGCAGTGCGCCCAGCCTGGGTGACCGAGTCCCCTATGTGATCATTGGTGCTGCTAAGGGTGTGGCCGCCT ACATGAAGTCGGAGGACCCCCTGTTTGTGCTGGAGCACAGCCTGCCCATCGACACTCAGTACTACCTGGA GCAGCAGCTGGCCAAGCCGCTCTTGCGCATCTTTGAGCCCATCCTGGGTGAGGGCCGTGCAGAGTCTGTG CTGCTGCGCGGTGACCACACACGATGCAAGACTGTGCTCACCAGCAAGGTGGGCGGCCTCTTGGCCTTCA CCAAGCGCCGCAACTGTTGCATTGGCTGCCGCTCCGTAATCGACCATCAAGGAGCCGTGTGTAAGTTCTG TCAGCCACGGGAGTCGGAGCTCTATCAGAAGGAGGTGTCACACCTGAATGCCTTGGAAGAACGGTTCTCT CGCCTCTGGACACAGTGTCAACGCTGCCAGGGCAGCTTGCATGAGGACGTCATCTGTACCAGCCGTGACT GTCCCATCTTCTACATGCGCAAGAAGGTGCGCAAGGACCTGGAAGACCAGGAACGGCTGCTGCAGCGCTT TGGACCGCCCGGCCCTGAGGCCTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217545 protein sequence
Red=Cloning site Green=Tags(s) MDCKRRQGPGPGVPPKRARGHLWDEDEPSPSQFEANLALLEEIEAENRLQEAEEELQLPPEGTVGGQFST ADIDPRWRRPTLRALDPSTEPLIFQQLEIDHYVGSAPPLPEGPPPSRNSVPILRAFGVTDEGFSVCCHIQ GFAPYFYTPAPPGFGAEHLSELQQELNAAISRDQRGGKELSGPAVLAIELCSRESMFGYHGHGPSPFLRI TLALPRLMAPARRLLEQGVRVPGLGTPSFAPYEANVDFEIRFMVDADIVGCNWLELPAGKYVRRAEKKAT LCQLEVDVLWSDVISHPPEGQWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPFLR LALTLRPCAPILGAKVQSYEREEDLLQAWADFILAMDPDVITGYNIQNFDLPYLISRAQALKVDRFPFLG RVTGLRSNIRDSSFQSRQVGRRDSKVISMVGRVQMDMLQVLLREHKLRSYTLNAVSFHFLGEQKEDVQHS IITDLQNGNEQTRRRLAVYCLKDAFLPLRLLERLMVLVNNVEMARVTGVPLGYLLTRGQQVKVVSQLLRQ AMRQGLLMPVVKTEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLRPGAAQKLGL KPDEFIKTPTGDEFVKSSVRKGLLPQILENLLSARKRAKAELAQETDPLRRQVLDGRQLALKVSANSVYG FTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTVENGYDANAKVVYGDTDSVMCRFGVSSVAEAM SLGREAANWVSSHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRSDAHDKMDCKGLEAVRRDNCPLVAN LVTSSLRRILVDRDPDGAVAHAKDVISDLLCNRIDISQLVITKELTRAAADYAGKQAHVELAERMRKRDP GSAPSLGDRVPYVIIGAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAESV LLRGDHTRCKTVLTSKVGGLLAFTKRRNCCIGCRSVIDHQGAVCKFCQPRESELYQKEVSHLNALEERFS RLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQERLLQRFGPPGPEAW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011131 |
| ORF Size | 3318 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011131.2 |
| RefSeq Size | 3428 bp |
| RefSeq ORF | 3318 bp |
| Locus ID | 18971 |
| UniProt ID | P52431 |
| MW | 123.7 kDa |
| Gene Summary | As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities. Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4. Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated. Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation. Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223588 | Pold1 (untagged) - Mouse polymerase (DNA directed), delta 1, catalytic subunit (Pold1), (10ug) |
CNY 14,160.00 |
|
| MG217545 | Pold1 (tGFP-tagged) - Mouse polymerase (DNA directed) delta 1 catalytic subunit (Pold1), (10ug) |
CNY 12,160.00 |
|
| MR217545L3 | Lenti ORF clone of Pold1 (Myc-DDK-tagged) - Mouse polymerase (DNA directed), delta 1, catalytic subunit (Pold1) |
CNY 12,920.00 |
|
| MR217545L4 | Lenti ORF clone of Pold1 (mGFP-tagged) - Mouse polymerase (DNA directed), delta 1, catalytic subunit (Pold1) |
CNY 12,920.00 |
