Sec22a (NM_133704) Mouse Tagged ORF Clone
CAT#: MR217787
- TrueORF®
Sec22a (Myc-DDK-tagged) - Mouse SEC22 vesicle trafficking protein homologe A (S. cerevisiae) (Sec22a)
ORF Plasmid: tGFP
"NM_133704" in other vectors (4)
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CNY 2,400.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1810005C06Rik; Sec22l2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR217787 representing NM_133704
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTATGATTTTGTCTGCCTCAGTCATTCGTGTGAGGGATGGACTACCGCTGTCTGCATCCACTGATT ATGAGCAGAGCACAGGAATGCAGGAGTGCAGAAAGTACTTTAAGATGCTGTCCAGGAAACTTGCTCAATT TCCTGATAGATGTACACTGAAAACGGGGCGCTATAACATTAATTTCATTAGCTCTCTGGGAGTGAGCTAC ATGATGTTGTGCAGTGAGAATTATCCAAATGTTCTCGCCTTCTCTTTCCTGGATGAGCTTCAGAAGGAGT TCATTACTACTTATAACATGATGAAGACAAATACTGCTGTCAGACCATACTGTTTCATTGAATTTGATAA CTTCATTCAGAGGACCAAGCAGCGATATAATAATCCCAGGTCTCTTTCAACAAAAATAAATCTGTCTGAC ATGCAGATGGAAATCAAGCTGAGGCCCCCTTATCAAATCCCAATGTGTGAACTGGGATCAGCAAATGGAG TCACATCTGCATTTTCTGTTGACTGTAAAGGTGCTGGTAAGATTTCTTCTGCCCATCAGCGACTAGAACC AGCAACTCTGTCAGGGATTGTGGCGTTTATCCTTAGTCTTTTATGTGGAGCTCTGAATTTAATTCGAGGC TTTCATGCCATAGAAAGCCTCCTGCAGAGTGATGGTGAGGATCTTAATTACATTATTGCATTTTTCCTTG GGACAGCAGCCTGCCTTTACCAGTGTTATTTACTTGTGTACTACACCAGCTGGCGGAATGTCAAGTCATT TCTGACTTTCGGCTTAATCTGTCTGTGCAACATGTATCTCTACGAACTGCGCAACCTCTGGCAGCTTTTC TTTCATGTGACTGTGGGAGCGTTTGTTACACTGCAGATCTGGCTGAGGCAAGCCCAAGGCAAGGCTCCTG ACCATGATGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217787 representing NM_133704
Red=Cloning site Green=Tags(s) MSMILSASVIRVRDGLPLSASTDYEQSTGMQECRKYFKMLSRKLAQFPDRCTLKTGRYNINFISSLGVSY MMLCSENYPNVLAFSFLDELQKEFITTYNMMKTNTAVRPYCFIEFDNFIQRTKQRYNNPRSLSTKINLSD MQMEIKLRPPYQIPMCELGSANGVTSAFSVDCKGAGKISSAHQRLEPATLSGIVAFILSLLCGALNLIRG FHAIESLLQSDGEDLNYIIAFFLGTAACLYQCYLLVYYTSWRNVKSFLTFGLICLCNMYLYELRNLWQLF FHVTVGAFVTLQIWLRQAQGKAPDHDV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_133704 |
ORF Size | 921 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_133704.4, NP_598465.2 |
RefSeq Size | 3992 bp |
RefSeq ORF | 924 bp |
Locus ID | 317717 |
UniProt ID | Q8BH47 |
MW | 35.5 kDa |
Gene Summary | May be involved in vesicle transport between the ER and the Golgi complex.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
The iRhom homology domain is indispensable for ADAM17-mediated TNFα and EGF receptor ligand release
,null,
Cellular and Molecular Life Sciences
,PubMed ID 33950315
[Sec22a]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC214356 | Sec22a (untagged) - Mouse SEC22 vesicle trafficking protein homologe A (S. cerevisiae) (Sec22a), (10ug) |
CNY 3,990.00 |
|
MG217787 | Sec22a (tGFP-tagged) - Mouse SEC22 vesicle trafficking protein homologe A (S. cerevisiae) (Sec22a), (10ug) |
CNY 2,850.00 |
|
MR217787L3 | Lenti ORF clone of Sec22a (Myc-DDK-tagged) - Mouse SEC22 vesicle trafficking protein homologe A (S. cerevisiae) (Sec22a) |
CNY 4,750.00 |
|
MR217787L4 | Lenti ORF clone of Sec22a (mGFP-tagged) - Mouse SEC22 vesicle trafficking protein homologe A (S. cerevisiae) (Sec22a) |
CNY 4,750.00 |