Pdxp (NM_020271) Mouse Tagged ORF Clone
CAT#: MR217795
- TrueORF®
Pdxp (Myc-DDK-tagged) - Mouse pyridoxal (pyridoxine, vitamin B6) phosphatase (Pdxp)
ORF Plasmid: tGFP
"NM_020271" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,400.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1600027H05Rik; AB041662; PLPP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR217795 representing NM_020271
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCGCTGCGAGCGGCTGCGCGGCGCGGCCCTGCGCGACGTGCTGGGCCAGGCGCAGGGAGTCCTGT TCGACTGCGACGGAGTGCTGTGGAACGGCGAACGCATCGTGCCGGGCGCCCCTGAGCTGCTGCAGCGGCT GGCGCGGGCCGGCAAGAACACGCTGTTCGTGAGCAACAACAGCCGGCGCGCGCGGCCCGAGCTGGCGCTG CGCTTCGCCCGCCTGGGCTTCGCGGGGCTGCGCGCCGAGCAGCTCTTCAGCTCCGCGCTGTGTGCCGCGC GCCTCCTGCGCCAGCGGCTGTCCGGGCCGCCCGACGCATCGGGCGCAGTGTTCGTGCTGGGAGGCGAGGG GCTGCGCGCTGAGCTGCGCGCCGCGGGGCTGCGCCTGGCGGGGGACCCCGGCGAGGACCCGCGCGTGCGC GCCGTGCTCGTAGGCTACGACGAGCAGTTTTCCTTCTCCAGGTTGACCGAGGCGTGCGCGCACCTGCGCG ACCCCGACTGCCTGCTCGTGGCCACCGATCGCGACCCTTGGCACCCGCTCAGCGACGGAAGCCGGACCCC CGGTACTGGAAGCCTGGCTGCCGCGGTGGAGACAGCCTCGGGACGCCAGGCCCTGGTGGTGGGCAAGCCC AGCCCTTACATGTTCCAGTGCATCACAGAAGACTTCAGCGTGGACCCCGCCCGCACGCTGATGGTGGGGG ACCGCCTGGAGACCGACATACTCTTTGGCCACCGCTGCGGCATGACCACCGTGCTCACGCTCACGGGCGT CTCCAGCCTCGAAGAAGCCCAGGCCTACCTGACGGCTGGCCAGCGTGACCTTGTGCCTCACTACTATGTG GAGAGCATTGCGGACTTGATGGAGGGGCTGGAGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217795 representing NM_020271
Red=Cloning site Green=Tags(s) MARCERLRGAALRDVLGQAQGVLFDCDGVLWNGERIVPGAPELLQRLARAGKNTLFVSNNSRRARPELAL RFARLGFAGLRAEQLFSSALCAARLLRQRLSGPPDASGAVFVLGGEGLRAELRAAGLRLAGDPGEDPRVR AVLVGYDEQFSFSRLTEACAHLRDPDCLLVATDRDPWHPLSDGSRTPGTGSLAAAVETASGRQALVVGKP SPYMFQCITEDFSVDPARTLMVGDRLETDILFGHRCGMTTVLTLTGVSSLEEAQAYLTAGQRDLVPHYYV ESIADLMEGLED TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_020271 |
ORF Size | 876 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020271.3, NP_064667.2 |
RefSeq Size | 2018 bp |
RefSeq ORF | 879 bp |
Locus ID | 57028 |
UniProt ID | P60487 |
MW | 31.5 kDa |
Gene Summary | Protein serine phosphatase that dephosphorylates 'Ser-3' in cofilin and probably also dephosphorylates phospho-serine residues in DSTN. Regulates cofilin-dependent actin cytoskeleton reorganization. Required for normal progress through mitosis and normal cytokinesis. Does not dephosphorylate phospho-threonines in LIMK1. Does not dephosphorylate peptides containing phospho-tyrosine (By similarity). Pyridoxal phosphate (PLP) phosphatase, which also catalyzes the dephosphorylation of pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), with order of substrate preference PLP > PNP > PMP (By similarity) (PubMed:24338473, PubMed:24338687).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210114 | Pdxp (untagged) - Mouse pyridoxal (pyridoxine, vitamin B6) phosphatase (Pdxp), (10ug) |
CNY 3,990.00 |
|
MG217795 | Pdxp (tGFP-tagged) - Mouse pyridoxal (pyridoxine vitamin B6) phosphatase (Pdxp), (10ug) |
CNY 2,850.00 |
|
MR217795L3 | Lenti ORF clone of Pdxp (Myc-DDK-tagged) - Mouse pyridoxal (pyridoxine, vitamin B6) phosphatase (Pdxp) |
CNY 4,750.00 |
|
MR217795L4 | Lenti ORF clone of Pdxp (mGFP-tagged) - Mouse pyridoxal (pyridoxine, vitamin B6) phosphatase (Pdxp) |
CNY 4,750.00 |