Ehbp1l1 (NM_001114597) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	G430002G23Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR218140 representing NM_001114597 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTTCAGTGTGGAAGCGCCTGCAGCGGGTTGGCAAGCGGGCCGCCAAGTTCCAGTTTGTGGCGTGTT ACCATGAACTGGTGTTGGAGTGCACCAAGAAGTGGCAGCCTGACAAGCTGGTGGTGGTATGGACTCGGCG GAACCGAAGGATCTGCTCCAAGGCCCACAGCTGGCAGCCTGGCATCCAGAACCCATACCGTGGCACCGTG GTATGGATGGTTCCTGAGAACGTGGACATCTCGGTGACCTTGTACAGGGACCCTCATGTGGACCAGTATG AAACCAAAGAGTGGACTTTTATTATTGAAAATGAGTCCAAGGGACAGCGGAAGGTACTGGCCACAGTTGA TGTGAACCTAGCCCACCATGCAGGGCCTGTGCCTGCTCAGGTTCCACTTCGACTGCGGCTGAAGCCCAAA TCTGTGAAGGTGGTGCATGCCGAACTGAGCCTCACCCTTTCTGGGGTGCTGCTGCGAGAAGGCCGTGCCA CGGATGATGACATGCAGAGTCTGGCCAGCCTCATGAGTGTGAAGCCTAGTGACGTGGGAAACCTGGATGA CTTTGCTGAGAGTGATGAGGAGGAAGCTAATGGCCCTGGGGCTCCTGAGGTCCGGACTCGAGGCCCCCAG TCAGATCTGTCTCGAGAGCTGAAGACACTCTGTGAAGAAGAAGATGAAGGCCACATACGGCCCCAGCAGG CAGCTGCCAGACCCTCTAGTGCTGAAGACACCAGCCCTGCCCCAGTGAGTGCCCCTGCACCCCCGGTCAG GGCCTTCCGGGGCCAGGGGTCAGAACCAGCTGCTATAACCGGGGGCCAGGTGGGGCCTGAAACCCCAGAG CCCCCGCCATCCCCACCAGAGACAAGGTCCACTGGGCAGCCAGGCCAGACCATGGTCCCCACCCCAGCCC CTCGGCTCCGGAAAGGCTCTGATGCACCCTCGTCCCCAGTCCCCTGCAGTGGGGATGAGGTCCCCAATAC CTCAGAGGATCCTCCAACAGGAATGGGCTCTTCTGGGGAGACCCAGGCTCAGATAAGCTCTCAGGAAGGG ACAGAGGCCCATGAAGCCAGGCCAGAACCAGACATTGAGGTCAGAGGCTCCAAAGATTCTCTGGGAGGAG AAAGATCCAAAGTTGAAGAGGAGGACACTGGGGACAGGCCGGGGGCTAGTGGGGCAGGGAATAGAGAGAA GAACACTAAGAAGTCGGACACCACAGCCGGCGAGGCTGGAGAGAGCTCAGAACTTCATCAAGTAGATGCT GAGCACAAGTCAAAGGTTCAACACAGAGCCACAGAGGGACCAGAGGCTGCAGGGCCGACGCCCAAGGCAA GGCTTGGGGACACTCCTGAGGCCCCTCCTAGGAGTGCTCAGAGGAGGATGGGGGTTAGGACCCAGGAAGA GGCTCCCAGTGACCTGAACCCACCTCCAGCTGAGCCTGAAGAACATCTAGGGGACCTCAGGGATGCCAGG CCTGCAGGCCAGGAGAAAGGAAGTGCAGAGGTGAGGGGTAAAGTCCCTGCTATTGGGAGGGCAGGCCCGG AGCAGGGCTCCTCTGCTAGAGCAGCAAGTGCTGGGCCCCAGGTGAGCTGCGTTCAGACAGTCCCATCAGA TGGCCAGGGGGTGAAATCTAGAGATCAGAGGGCTCAGGAGGCAGAAGTTGGGGAGTCAAGGGTTCTAGAA ACAGAGGCTGAGTGGGTGCCATGGGAGGTTATAGGGACATCAAAGACAGATGCTGGGATACCAGAATCCC TGGATACAGAGGCTGGCACAGCAGAGTCTGAAATATTGGAGGCCCAGGAGTCAGAAGCAGCAAGGTCAGA GGGCCTGGAACCCGAGGCTGCTGGGACAGCAGAGTCTGAGGTGCTGAGGACCCAGAATAACGAGATCGTG GTTCTGGGGATGCCGAGGACAGGGCCTGAGATAAGGGAGCCTGAGGAATTTGGGGAAACAGAAGTTGGGG GTTTTACAGTTCCAGATACCAAGACAGTGATAGCAGAAACTGAGATATTAGAAACCCAGGGGGTAGTGGA TGGTGAGGCTGCGGTGCTGAAGACACAGGCTGAGATATTGGAAACCCAGAAGACAGAGGCTGGGGAGGCA GAAGCGGGAACACTGGAGTCCCAGAAGGTAGCAGCCGAAGGTTTGGGGGCTCCAGAAGTGGGGGCAGAGA TGGCAGAGGCTGAGAAATTGGGGGTGCAGGAGACAGAGGTGGAGATTTGGAGGATTCCAAGAATAGAAAC CGAAACAGCAGGAACTGAGACATTGGGGATCCACAAAATAGGGCCCCCACAGATGCAACCTAGACTAGTA GGGGACCAGGAGACCGATGTTTCAGTGATGGAGACAGCAGAAGATGCTATACTGGGGACCCGGGAGATAT CAGCAGGTTGTGGAGTACTTTTGATAGAAGCAAAGATACCAGAATCCAAGATTGACAGATCCCTGGAGAC AGAGGAAGGAGATTTAGGGGTTCTAGAGGTAGATACTGGGATAGCAGAGGCTAAGATCTTAGGGATACCA GAGAGAGCACCAGGGGTTCAGAAGGCTCTGGGAGCTGGAACTGAGGTAGCCAGGGTCTTGGAAGCAGAGG CTAGCTCTTCAGAAGTCCCAGAGACAGACGCTGAAGAAGCTGAGACACTGCAGGCCAAGGAGCGATCTGA GAGTTCTGTGGCGCTGAGAGTAGTGGCCAACCTACCAGAATCTGAGCTCTTAGGGACCCAGAAGAAGGTG GGAGGCACTGGGATATCACAGAGAGAGGTTAGAGAGGCAGAGACCGAAATCCCAAAGACCCAGGAAATAT CATCTGAGGGTTCAGGAGTTCCAGACTTGGAGGCTAAGATGGAAGAGTCTGGGAGGAAAATGGAGATTTG GGAGACCCCAGAGGTAGAGAAAGTGAATTCTGAGTTATTTGGAACCCAGAAAGGCTCAGAGATCCCAGAA CTAGAAACTAAGACCATAAAGTCTGAGATTCTGGACACCCAGGAGACAGAAGTAGGGGGCTTGGGGCTTC GTCGAGGAGAGGCTGAGAAAGCAGAAGCTGAGATGTTAGAAACCCAAAAGACGGAAGCAAAGACAGCTCG GGATGAAGAAATAGAACTAGTGGATCCTGGGGTCTCCAGTCCAGAGGCTGAGGCACTGAGGGTCCAGTGG GCAGGGACTGTAGTTTTGGAGTCTGGGGAAGTAAAGGCTGACATTTTGGGGGTCCAGAAGCCTGGATCTT GGGGAGCTCTTAAATATGAGGCCTTAGATGTCCCCGTAACTAAGCAAAGGCTTTCTGGGGCCAAGGAAGT GGTGCCAGAGGTACCCAGAGCACAAGAGCCAGAAACTAAAGTTTTGGGGATAGTAGAGGCCAAATCTTGG ACTTTGGGGCAGCAGGAAGCAGAGATGGAGGGGTTTGAGTCTCCAGAGAATAAATCTAATATTTTTGAGG CCCAGGAAGCAGATTCTGGGGTCTTGGGAACCATGAAGGGGAAAGAAGCTGTTGAAAGCCTCGAGGAGGC TGGCCTGAGTAAGGCACAAGTGGCCAGTGAGGCAGGGGCTGGGGTGCCCAGGCCCTCAGGGGCCTCTTCC CTAGAGGAACCTGAAGAGGACAGGAGGCTGCCGGGCAGCCAGGCACCACCTACCCTGGTCAGCTCCAGCC AGTCCCTGTTGGAGTGGTGCCAAGAGGTCACCAACGGCTACCGTGGTGTCTGCATCACCAACTTCACCAC GTCCTGGCGCAATGGCCTGGCCTTCTGTGCTATTTTACATCGATTCTACCCAGACAAGATCGATTATTTC TCCCTTGATCCCCTCAACATCAAACAGAACAACAAGCAGGCTTTTGATGGCTTCGCTGCCCTGGGTGTGT CTCGGCTGCTCGAGCCGGCGGACATGGTACTTCTGTCCGTACCTGACAAGCTCATCGTCATGACGTACCT GTGCCAGATCCGTGCCTTCTGCACTGGGCAGGAGCTGCAGCTGGTGCAACTGGAGGGCGGCGGTGGCTCT GGCACTTATCGTGTGGGCAACGCCCAGCCGAGCCTGCCCGACTGTCTGGACGCAGGAGACCTGGCGCAGC GATTACGCGAGCATGGGGCTGAAGTGCCCACAGAACCTAAGGAGGCTGTGAACCGCGGGACTGGGGCAAT ACCAAAGGTGGCCTCCAGGGACACGGACCTGAGCTGCTCCTCTAAGGATGGGGAGGCAGAGGTTGCCCAG GAAGCAATCCCTCAAGAGGCGCCCACCGACGGCCCTAGAGCCAGGTCGTCCACAACCCCGGTGGTCCCTG CAGAGGGGCTGGTGAACGGAGTGGGGGCGTCAGGTGGTGTGAGTGGTGTGAGACTGAGACGGTCCTCTGT CAATGGGGAGGCTGGGCCAGTACCTCCACCCCGAGCACATGGCTCTTTCTCCCACGTGCGGGACGCCGAT TTGCTAAAGAAGAGGCGATCGAGGTTAAGGAATAGCAACTCTTTCTCTGTGGATGACCAGGACTCTGGAG CTGCAGTTGGAGCAGGGCCTGCAGGGCCTGGAGCTGTGGAAGGTCCAAACCCTGCCTCCAGCCCTGACGC TAACCCACTCCCAGCCCCAGCCCCACAGCAGCCGCCCGGTGGGCCCCCTCCTACTGAGGAGTCATCACCC AGCCTGGGGGAAGAGACAGGCCTGCAACGGTTCCAGGACACAAGTCAGTACGTGTGCGCAGAGCTGCAAG CCCTGGAGCAGGAACAGGGACAGATAGATGGGAGGGCCGCTGAGGTGGAGAAGCAGCTGAGGAGCCTCAT GGAATCAGGTGCCAACAGGCTGCAGGAGGAGGTGCTGATTCAGGAATGGTTCACCCTGGTCAACAAGAAG AATGCGCTCATCCGGAGGCAGGACCAGCTGCAGCTGCTCATCGAGGAGCAGGACTTGGAGCGGAGGTTTG AACTGCTGAGCCGAGAGTTGCGGGCCATGCTGGCCATTGAAGAGTGGCAGAAAACAGTTGCGCAGCAGCA CCGCGAGCAACTCCTGTTGGAGGAGCTGGTGTCTCTGGTGAACCAGAGGGATGAACTGGTCCGGGACCTG GACCAGAAGGAACGGATCGCTCTGGAGGAAGATGAGCGCCTAGAACGAGGCCTGGAGCAGAGACGTCGCA AGGTGAGCCGGCAGCTGAGCAGGCGGGAACGCTGTACCCTGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR218140 representing NM_001114597 Red=Cloning site Green=Tags(s) MTSVWKRLQRVGKRAAKFQFVACYHELVLECTKKWQPDKLVVVWTRRNRRICSKAHSWQPGIQNPYRGTV VWMVPENVDISVTLYRDPHVDQYETKEWTFIIENESKGQRKVLATVDVNLAHHAGPVPAQVPLRLRLKPK SVKVVHAELSLTLSGVLLREGRATDDDMQSLASLMSVKPSDVGNLDDFAESDEEEANGPGAPEVRTRGPQ SDLSRELKTLCEEEDEGHIRPQQAAARPSSAEDTSPAPVSAPAPPVRAFRGQGSEPAAITGGQVGPETPE PPPSPPETRSTGQPGQTMVPTPAPRLRKGSDAPSSPVPCSGDEVPNTSEDPPTGMGSSGETQAQISSQEG TEAHEARPEPDIEVRGSKDSLGGERSKVEEEDTGDRPGASGAGNREKNTKKSDTTAGEAGESSELHQVDA EHKSKVQHRATEGPEAAGPTPKARLGDTPEAPPRSAQRRMGVRTQEEAPSDLNPPPAEPEEHLGDLRDAR PAGQEKGSAEVRGKVPAIGRAGPEQGSSARAASAGPQVSCVQTVPSDGQGVKSRDQRAQEAEVGESRVLE TEAEWVPWEVIGTSKTDAGIPESLDTEAGTAESEILEAQESEAARSEGLEPEAAGTAESEVLRTQNNEIV VLGMPRTGPEIREPEEFGETEVGGFTVPDTKTVIAETEILETQGVVDGEAAVLKTQAEILETQKTEAGEA EAGTLESQKVAAEGLGAPEVGAEMAEAEKLGVQETEVEIWRIPRIETETAGTETLGIHKIGPPQMQPRLV GDQETDVSVMETAEDAILGTREISAGCGVLLIEAKIPESKIDRSLETEEGDLGVLEVDTGIAEAKILGIP ERAPGVQKALGAGTEVARVLEAEASSSEVPETDAEEAETLQAKERSESSVALRVVANLPESELLGTQKKV GGTGISQREVREAETEIPKTQEISSEGSGVPDLEAKMEESGRKMEIWETPEVEKVNSELFGTQKGSEIPE LETKTIKSEILDTQETEVGGLGLRRGEAEKAEAEMLETQKTEAKTARDEEIELVDPGVSSPEAEALRVQW AGTVVLESGEVKADILGVQKPGSWGALKYEALDVPVTKQRLSGAKEVVPEVPRAQEPETKVLGIVEAKSW TLGQQEAEMEGFESPENKSNIFEAQEADSGVLGTMKGKEAVESLEEAGLSKAQVASEAGAGVPRPSGASS LEEPEEDRRLPGSQAPPTLVSSSQSLLEWCQEVTNGYRGVCITNFTTSWRNGLAFCAILHRFYPDKIDYF SLDPLNIKQNNKQAFDGFAALGVSRLLEPADMVLLSVPDKLIVMTYLCQIRAFCTGQELQLVQLEGGGGS GTYRVGNAQPSLPDCLDAGDLAQRLREHGAEVPTEPKEAVNRGTGAIPKVASRDTDLSCSSKDGEAEVAQ EAIPQEAPTDGPRARSSTTPVVPAEGLVNGVGASGGVSGVRLRRSSVNGEAGPVPPPRAHGSFSHVRDAD LLKKRRSRLRNSNSFSVDDQDSGAAVGAGPAGPGAVEGPNPASSPDANPLPAPAPQQPPGGPPPTEESSP SLGEETGLQRFQDTSQYVCAELQALEQEQGQIDGRAAEVEKQLRSLMESGANRLQEEVLIQEWFTLVNKK NALIRRQDQLQLLIEEQDLERRFELLSRELRAMLAIEEWQKTVAQQHREQLLLEELVSLVNQRDELVRDL DQKERIALEEDERLERGLEQRRRKVSRQLSRRERCTLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001114597
ORF Size	5154 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001114597.1, NP_001108069.1
RefSeq Size	6411 bp
RefSeq ORF	5157 bp
Locus ID	114601
MW	184.8 kDa
Gene Summary	May act as Rab effector protein and play a role in vesicle trafficking (By similarity). Involved in apical-directed transport in polarized epithelial cells; the functions seems to implicate Rab8, BIN1 and possibly DNM1 (PubMed:26833786).[UniProtKB/Swiss-Prot Function]

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