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Mmp20 (NM_013903) Mouse Tagged ORF Clone
CAT＃: MR218343
- TrueORF®
Mmp20 (Myc-DDK-tagged) - Mouse matrix metallopeptidase 20 (enamelysin) (Mmp20)
ORF Plasmid: tGFP
"NM_013903" in other vectors (4)
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CNY 3,656.00
CNY 4,280.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218343 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGTGCTACCTGCCTCTGGCCTTGCTGTCCTTGTCACAGCTTTAAAGTTTGCCACTGCAGACCCCA ACCTACTGGCAGCCACCCCTAGGACCTTCAGGAGCAACTACCACCTTGCACAGGCCTATCTTGACAAGTA TTATACAAAGAAAGGAGGCCCCCAGGCTGGCGAGATGGTGGCAAGAGAAAGCAACCCCATGATCAGGAGG ATTAAGGAGCTACAAATATTCTTTGGCCTCAAAGTCACTGGGAAGTTGGACCAGAATACAATGAATGTGA TCAAGAAGCCTCGCTGTGGAGTTCCTGATGTGGCTAACTACCGCCTCTTCCCAGGTGAACCCAAATGGAA AAAAAATATTTTGACATACAGAATCTCTAAGTACACACCCTCCATGAGTCCCACTGAGGTGGACAAGGCA ATACAGATGGCCCTGCATGCGTGGAGTACAGCTGTCCCTCTGAACTTCGTTAGAATAAACTCTGGGGAAG CAGATATCATGATATCTTTTGAAACTGGAGATCATGGGGATTCCTACCCATTTGATGGGCCTCGAGGGAC TCTGGCCCATGCATTTGCTCCTGGAGAAGGCCTGGGAGGAGACACACATTTCGACAATGCTGAGAAGTGG ACTATGGGAACGAATGGGTTCAATTTATTCACCGTTGCTGCTCATGAATTTGGCCATGCTCTGGGCCTGG GCCATTCCACAGATCCATCAGCACTGATGTACCCAACTTATAAGTACCAGAACCCCTATAGATTTCATCT CCCCAAGGATGATGTGAAAGGGATCCAAGCATTATACGGACCCCGGAAAATATTCCCAGGAAAGCCCACC ATGCCCCATATCCCACCTCACAAGCCATCCATCCCCGACCTCTGCGACTCCAGCTCGTCCTTTGATGCAG TGACAATGTTGGGAAAGGAGCTTCTGTTCTTCAAGGATAGGATTTTCTGGAGAAGGCAGGTGCACTTGCC AACAGGGATTCGGCCCAGCACCATCACCAGCTCCTTTCCCCAGCTAATGTCCAATGTGGACGCGGCTTAC GAAGTGGCTGAACGAGGCATTGCTTTCTTCTTCAAAGGTCCTCACTACTGGGTCACTCGAGGATTCCATA TGCAAGGTCCTCCACGGACAATTTATGACTTCGGGTTCCCAAGGCATGTGCAACGAATAGATGCTGCTGT CTACCTCAAGGAGCCTCAGAAGACCCTTTTCTTTGTGGGAGAGGAATACTACAGCTATGATGAAAGAAAA AAGAAAATGGAAAAAGACTACCCAAAAAACACTGAAGAAGAGTTTTCAGGAGTGAGTGGCCATATAGATG CTGCTGTGGAACTGAATGGCTACATTTACTTCTTTTCAGGACGGAAAACATTCAAGTATGATACAGAGAA GGAAGACGTGGTTAGTGTGGTAAAATCCAGTTCCTGGATTGGCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218343 protein sequence
Red=Cloning site Green=Tags(s) MKVLPASGLAVLVTALKFATADPNLLAATPRTFRSNYHLAQAYLDKYYTKKGGPQAGEMVARESNPMIRR IKELQIFFGLKVTGKLDQNTMNVIKKPRCGVPDVANYRLFPGEPKWKKNILTYRISKYTPSMSPTEVDKA IQMALHAWSTAVPLNFVRINSGEADIMISFETGDHGDSYPFDGPRGTLAHAFAPGEGLGGDTHFDNAEKW TMGTNGFNLFTVAAHEFGHALGLGHSTDPSALMYPTYKYQNPYRFHLPKDDVKGIQALYGPRKIFPGKPT MPHIPPHKPSIPDLCDSSSSFDAVTMLGKELLFFKDRIFWRRQVHLPTGIRPSTITSSFPQLMSNVDAAY EVAERGIAFFFKGPHYWVTRGFHMQGPPRTIYDFGFPRHVQRIDAAVYLKEPQKTLFFVGEEYYSYDERK KKMEKDYPKNTEEEFSGVSGHIDAAVELNGYIYFFSGRKTFKYDTEKEDVVSVVKSSSWIGC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013903 |
| ORF Size | 1449 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013903.1, NM_013903.2, NP_038931.1 |
| RefSeq Size | 3276 bp |
| RefSeq ORF | 1449 bp |
| Locus ID | 30800 |
| UniProt ID | P57748 |
| MW | 54.4 kDa |
| Gene Summary | This gene encodes a member of the matrix metalloproteinase family of zinc-dependent endopeptidases capable of degrading extracellular matrix proteins. This gene is expressed specifically in the ameloblasts and odontoblasts, and the encoded protein is an inactive zymogen that requires proteolytic removal of a N-terminal propeptide to become enzymatically active. Mice lacking the encoded protein display an amelogenesis imperfecta phenotype. [provided by RefSeq, Oct 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216660 | Mmp20 (untagged) - Mouse matrix metallopeptidase 20 (enamelysin) (Mmp20), (10ug) |
CNY 6,270.00 |
|
| MG218343 | Mmp20 (tGFP-tagged) - Mouse matrix metallopeptidase 20 (enamelysin) (Mmp20), (10ug) |
CNY 4,750.00 |
|
| MR218343L3 | Lenti ORF clone of Mmp20 (Myc-DDK-tagged) - Mouse matrix metallopeptidase 20 (enamelysin) (Mmp20) |
CNY 6,180.00 |
|
| MR218343L4 | Lenti ORF clone of Mmp20 (mGFP-tagged) - Mouse matrix metallopeptidase 20 (enamelysin) (Mmp20) |
CNY 6,180.00 |
