Psmf1 (NM_212446) Mouse Tagged ORF Clone
CAT#: MR218495
- TrueORF®
Psmf1 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) inhibitor subunit 1 (Psmf1)
ORF Plasmid: tGFP
"NM_212446" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AW048666; BC012260; PI31 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR218495 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGTCTCGAGGTTCTCTTCGCGTCGGCCGCTCCATCCATGAGCTGCCCGCAGGACGCACTCGTCT GCTTCCTGCACTGGGAAGTGGTGACAAACGGCTACTATGCCTTGGGCACGGGCGACCAGCCAGGTCCCAG TGATAAGAAATCAGAACTACTGCCAGCTAAGTGGAACAGCAATAAAGAACTGTATGCCCTCCGGTATGAG TCTAAGGATGGAGCCAGAAAACTCCTTCTGAAAGCTGTCTCTGTGGAGAACGGCATGATCATCAACGTGC TGGAACTTGGCACACAGCAGGTGGCAGACTTGACCCTGAACTTAGATGATTATATTGATGCAGAAGACCT GAGTGACTTCCACAGGACCTATAAGAACAGTGAGGAGCTTCGGTCTCAGATCAGATCTGGAATTATCACA CCTATCCATGAGCAGTGGGAGAAGGCTCGTGCAAACAGTCCTCCGAGGGAGTTCCCACCTGCCACTGCCA GAGAGGTGGACCCACTCCAGATTTCCTCACACCGTCCTCATACTAGCAGGCAGCCTGCTTGGCGTGATCC CCTGAGCCCATTTGCTGTCGGGGGAGATGACCTAGACCCTTTTGGGTGTCAGAGAGGTGGCATGATTGTG GATCCCCTGAGATCCGGCTTCCCAAGAGTACTTATTGACCCATCCTCAGGCCTCCCAAACCGGCTTCCTC CTGGTGCTGTGCCCCCAGGAGCTCGCTTTGACCCCTTTGGACCCATTGGGACCAGCCCATCTGGACCTAA CCCTGACCATCTTCCCCCACCGGGCTACGATGACATGTACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218495 protein sequence
Red=Cloning site Green=Tags(s) MAGLEVLFASAAPSMSCPQDALVCFLHWEVVTNGYYALGTGDQPGPSDKKSELLPAKWNSNKELYALRYE SKDGARKLLLKAVSVENGMIINVLELGTQQVADLTLNLDDYIDAEDLSDFHRTYKNSEELRSQIRSGIIT PIHEQWEKARANSPPREFPPATAREVDPLQISSHRPHTSRQPAWRDPLSPFAVGGDDLDPFGCQRGGMIV DPLRSGFPRVLIDPSSGLPNRLPPGAVPPGARFDPFGPIGTSPSGPNPDHLPPPGYDDMYL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_212446 |
ORF Size | 816 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_212446.2 |
RefSeq Size | 3542 bp |
RefSeq ORF | 816 bp |
Locus ID | 228769 |
UniProt ID | Q8BHL8 |
MW | 29.7 kDa |
Gene Summary | Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC212831 | Psmf1 (untagged) - Mouse proteasome (prosome, macropain) inhibitor subunit 1 (Psmf1), (10ug) |
CNY 2,400.00 |
|
MG218495 | Psmf1 (tGFP-tagged) - Mouse proteasome (prosome macropain) inhibitor subunit 1 (Psmf1), (10ug) |
CNY 2,850.00 |
|
MR218495L3 | Lenti ORF clone of Psmf1 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) inhibitor subunit 1 (Psmf1) |
CNY 4,750.00 |
|
MR218495L4 | Lenti ORF clone of Psmf1 (mGFP-tagged) - Mouse proteasome (prosome, macropain) inhibitor subunit 1 (Psmf1) |
CNY 4,750.00 |