Alpk3 (NM_054085) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AW319487; D330016D04; MAK; Midori; mKIAA1330
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR218500 representing NM_054085 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTCGCGGAGGGCCGCGGGCCGGGGCTGGGGCCTGGGTGGTCGAGCAGGAGCTGGTGGAGATAGCG AGGACGACGGGCCGGTGTGGACGCCCGGCCCAGCCAGTCGCAGCTACTTGCTTAGCGTGCGGCCAGAGGC TAGCTTATCAAGCAACCGGTTGTCTCACCCCAGCTCTGGAAGGAGCACCTTCTGCTCCATCATTGCTCAG CTCACAGAGGAGACCCAGCCACTGTTCGAGACCACACTCAAGTCCCGTGCCGTGTCCGAAGACAGTGACG TCAGGTTCACCTGCATTGTCACAGGATACCCAGAGCCAGAGGTGACCTGGTACAAGGATGACATAGAACT GGACCGTTACTGTGGCTTGCCAAAATACGAGATCACTCATCAAGGCAACCGTCACACCCTGCAGCTGTAC AGGTGTCAGGAAGAAGATGCTGCCATCTACCAAGCCTCTGCCCGGAACACCAAGGGCATCGTGTCCTGCT CAGGGGTCCTAGAGGTGGGCACTATGACGGAGTACAAGATTCACCAGCGCTGGTTCGCCAAGTTGAAGCG CAAGGCTGCAGCCAAGATGAGGGAGATTGAGCAGAGCTGGAAGCATGGAAAAGAGGCTTCAGGGGAGGCT GACACGCTTCTTCGCAAGATCAGCCCCGACCGCTTCCAAAGAAAGCGCCGACTGAGTGGAGTCGAAGAGG CTGTCCTCTCCACGCCAGTCAGGGAAATGGAGGAAGGCTCCTCAGCGGCTTGGCAGGAAGGAGAGACTGA GTCTGCTCAGCACCCGGGGTTGGGTTTGATCAACAGTTTTGCTCCTGGAGAGGCGCCCACCAATGGGGAG CCTGCTCCAGAGAACGGGGAAGACGAAGAGCGTGGCTTGCTGACATACATCTGCGAGGTCATGGAACTGG GGCCTCAGAACAGCCCTCCAAAGGAGTCTGGGGCTAAGAAGAAAAGGAAGGATGAGGAATCTAAACCAGG AGAGCAGAAGCTGGAGTTAGAAAAGGCAGAAGGGAGCCAGTGCTCTTCAGAAAACGTCGTCCCCAGTACA GACAAACCCAACTCCAGTAGAAGGGAGAAGTCCACGGATACACAGCCAGCTCAGACCCAGCCCAGAGGCC GGGTAGCACGGGGGCCTGGGATAGAAAGCACCAGGAAGACAGCCTCTGTCCTGGGCATTCAAGACAAGGT CCAGGATGTCCCCGCCCCCGCCCCTGCCCCGGTCCCCGCCCCAGCCCTGGCCCCGGCCCCTGTCCCAGTC CCTGCACCCACCCCTGTTCCAAGCCGCAGCTCAGAGCAGGTGTATTTCTCCCTGAAGGACATGTTCATGG AGACCACCCGGGCAGGCAGGTCCCAGGAAGAGGAAAAACCTCCACCTCCAAGTACCAGGGTAGCTGGAGA AAGTCCCCCAGGAAAGACACCAGTCAAGTCTAGACTGGAGAAGGTACCGATGGTCTCCAGCCAGCCCACA TCTTCCATGGTTCCCCCGCCCATTAAGCCTTTGAACAGGAAGAGATTTGCCCCTCCTAAATCCAAAGTGG AGTCAACTACTACCTCTCTCTCAAGTCAGACTTCAGAATCTATGGCCCAGAGCTTAGGGAAGGCTCTACC TTCAGCCTCTACCCAGGTCCCAACACCCCCTGCTCGACGGAGACACGGCACCCGAGATAGCCCCTTGCAA GGACAAACGAGCCACAAGACTCCAGGAGAGGCTCTGGAGTCCCCAGCAACCGTGGCTCCCACCAAGTCTG CCAACAGCAGCTCCGATACCGTCTCTGTTGATCACGACAGCTCTGGAAATCAAGGGGCCACGGAGCCCAT GGATACAGAAACTCAGGAAGATGGAAGGACACTTGTGGATGGGAGAACTGGAAGCAGGAAGAAAACACAC ACAGATGGAAAGCTGCAAGTGGATGGGAGGACTCAGGGAGACGGAGCACAAGACAGAGCACACGCTTCGC CAAGGACACAGGCAGGTGAGAAGGCACCGACGGACGTTGTGACACAAGGAAGTGAGAGGCCACAGTCAGA CAGGAGTTCATGGAAGAATTTGGTGACACAGAGAAGAGTGGATATGCAGGTAGGACAGATGCAGGCAGGT GAGAGGTGGCAGCAAGACCCTGGAGACGCAAGGATACAGGAGGAAGAAAAAGAGACACAGTCAGCAGCAG GCAGCATTCCTGTAGCTTTCGAAACCCAATCAGAGCAGTTGTCCATGGCCAGCCTCAGCTCACTTCCTGG AGCTCTCAAAGGCTCACCATCAGGATGCCCTAGAGAGTCCCAGGCTATAGAATGTTTTGAGAAGAGCACA GAGGCACCCTGTGTCCAAGAAAGATCTGACTTGATGCTGCGGTCTGAAGAGGCAGCCTTCAGAAGCCATG AGGATGGGCTGCTAGGCCCCCCATCAGGGAACCGTACCTACCCAACACAGTTGCCTCCCGAGGGGCACTC AGAGCATTTGGGAGGACAAACACATCAAAGGTCAGAGCAAGAGGACAGCCTGTCTCAGTGCCCCAAGAAG GAGCAGCCCCAGGAACCGCTTCATGTGGGTCTCTCTGGTGGGCATTCAACTGGCTTGAGTCAGGAGGTGC CTGCCATGCCTTCTCTTCCTGGGACGGGCCTAACCAGTAGTCTGCAGGAGGAGCTACCAGGCACCGCAGC TTCTCTGCACACAAACACAGATGTCCCCCTCCCCTCCAGGGACCAGGACTTTCCGAGTTCTGCTCCCACT CTGCAATTGGGGCCAGGGTCCCCCACTCAGAGTCACCCACCAGAAGCCATGGCTACTAGCAGTGAGGGAG CCTGTGCCAAGGAGCCAAATGTGGACGGGAGGTCCTCAGGTACCCGGAGCTGTGACCCTGGCCTTATAGA TTCCCTGAAGAACTACTTGCTTCTGCTGCTGAAGCTATCCAGTCCAGAGACAAGTGAAGCCAGGGCCGAG TCCCAGGAAGTGGCAGACACCGGGGGCTTAACCTCCTCCTCTACTCTGGTCCCCACCATGGAGGTGGCTG GGCTGAGTCCCAGGACGTCGAGGCGAATCCTGGAACGCGTGGAGAACAATCACTTAGTGCAGAGTGCACA GACCCTGTTGCTGAGCCCCTGCACCTCCCGCCGCCTTACTGGCCTTCTGGACCGTGAGGTACAGGCTGGC CAGCAGGCTCTGGCTGCTGCCCAGTGCTCTCGGGGCCCGTGCCCCACCCCCCTCACCATCCCTGCCATTG TGGTGGGTGAGGAAGGATCTGCGGGAGAGGATTCCGAGGAGAGGACTTCGCAGGAAAGTGACAAGAAGGG ACTGCTAGGGGAAGTGGAGGGGCACACAGTGGAAAGCAGAACCCAGGAGCCCTGCCAAGAAGAAGCAATG CCAGGGGAGGCTTTGACGGGTCTCCCTGCAGCTACACCCGAGGAACTGGCTCTGGGGGCCCGGAGGAAGA GGTTCCTCCCTAAGGTCAGAGCAGGGTCAGATGGAGAGGCAAACAAGGCTGAAGAAAGGGAGAGCCCCAC GGTTTCCCCCCGGGGACCCAGGAAGGGCCTGACACCTGGGTCACCAGGGACTCCAGGGCGGGAGAGACGG TCCCCTACCCAGGCCCGAAAAGCCAGCATGTTAGAGGTGCCTGGCGCAGAAGAAGAGCCTGCAACTGGAG ACTTGGTCTCCAGATCCAAAGACAGTGGCCTGGACTCAGAGCCTGCGGTGGATGAAGGCAAGCAGGAAGC TCTGGCCAAGCAAAGGAAAGCTAAGGACCTGCTAAAAGCCCCACAGGTGATCCGGAAAATTCGGGTGGAA CAGTTTCCAGATTCTTCTGGTAGTCTGAAGCTTTGGTGCCAGTTTTTCAACATTGTTAGTGACTCAGTCT TGACATGGGCGAAGGATCAGCACCCAGTGGGCGAAGTGAACAGGAGTGCAGGGGACGAGGGGCCAGCGGC TTTGGCCATCGTGCAGGCGTCTCCCACGGACTGTGGTGTGTATCGCTGTACCATCCAAAACGAGCATGGC TCAGCGTCCACTGACTTCTGCCTCAGCCCCGAGGTATTGTCCGGCTTCATCTCCAGAGAGGAAGGTGAAG TTGGAGAAGAGATTGAGATGACCCCCATGGTGTTTGCTAAGGGTCTGGCTGACTCTGGCTGCTGGGGGGA CAAGCTCTTTGGGCGATTGGTGAGCGAGGAACTTCGAGGGGGTGGACATGGCCTTCAGAAGGCGTCCCGG GCCAAGGTCATCTATGGGCTGGAACCCATCTTCGAATCTGGCCGCACGTGCATCATCAAAGTATCCAGCC TGCTTGTGTTCGGACCCAGCAGTGAGACCTCTCTTCTGGGCAGAAACTATGACGTCACTATCCAGGGATG CAAGATCCAGAACATGAGTCGAGAGTACTGCAAAATCTTTGCAGCTGAAGCCCGGGCGGCCTCTGGCTTC GGAGAGGTGCCCGAGATCATCCCACTCTACTTGATCTACCGGCCTGCAAACAATATACCATATGCAACCC TGGAGGAAGATCTGGGCAAGCCCCTGCAGACTTACTGTTCCAGGCAGTGGGGCTGTGCTGGGGCCCCCGC AGCAGCCAGCAGCTCCGAGGCCTTGCAGAAATGCCAAACCTTCCAGCACTGGCTGTATCAGTGGACAAAC GGCAGCTTTCTTGTCACAGATCTGACAGGAGCTGACTGGAAGATGACTGATGTACAGATTGCTACCAAAC TTCGAGGATACCAAGGCCTCAAGGAGAGCTGTTTTCCTGCCCTGCTGGACCAGTTTGCCTCTTCCCACCA GTGTAACACCTACTGTGACATGCTGGGGCTGAAGCCCCTCAAAGGCCCTGAGGCTGCCCACCCTCAAGCC AAGGCCAAAGGCTCCAAAAGTCCATCTGCTGGCAGAAAAGGCTCACAGCTGAGTCCTCAACCCCAGAAGA AAGGCCTTCCCAGTCCCCAGGGCTCCAGGAAGAGCGCTCCAAGCTCCAGGGCTACACTTCAGGCCTCCCA GGCAGCCACTGTTCAGTTACTGGGACAGCCTCCTGTCCAAGATGGGAGCTCTAAGGCCCAGAGCATGCGG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR218500 representing NM_054085 Red=Cloning site Green=Tags(s) MGSRRAAGRGWGLGGRAGAGGDSEDDGPVWTPGPASRSYLLSVRPEASLSSNRLSHPSSGRSTFCSIIAQ LTEETQPLFETTLKSRAVSEDSDVRFTCIVTGYPEPEVTWYKDDIELDRYCGLPKYEITHQGNRHTLQLY RCQEEDAAIYQASARNTKGIVSCSGVLEVGTMTEYKIHQRWFAKLKRKAAAKMREIEQSWKHGKEASGEA DTLLRKISPDRFQRKRRLSGVEEAVLSTPVREMEEGSSAAWQEGETESAQHPGLGLINSFAPGEAPTNGE PAPENGEDEERGLLTYICEVMELGPQNSPPKESGAKKKRKDEESKPGEQKLELEKAEGSQCSSENVVPST DKPNSSRREKSTDTQPAQTQPRGRVARGPGIESTRKTASVLGIQDKVQDVPAPAPAPVPAPALAPAPVPV PAPTPVPSRSSEQVYFSLKDMFMETTRAGRSQEEEKPPPPSTRVAGESPPGKTPVKSRLEKVPMVSSQPT SSMVPPPIKPLNRKRFAPPKSKVESTTTSLSSQTSESMAQSLGKALPSASTQVPTPPARRRHGTRDSPLQ GQTSHKTPGEALESPATVAPTKSANSSSDTVSVDHDSSGNQGATEPMDTETQEDGRTLVDGRTGSRKKTH TDGKLQVDGRTQGDGAQDRAHASPRTQAGEKAPTDVVTQGSERPQSDRSSWKNLVTQRRVDMQVGQMQAG ERWQQDPGDARIQEEEKETQSAAGSIPVAFETQSEQLSMASLSSLPGALKGSPSGCPRESQAIECFEKST EAPCVQERSDLMLRSEEAAFRSHEDGLLGPPSGNRTYPTQLPPEGHSEHLGGQTHQRSEQEDSLSQCPKK EQPQEPLHVGLSGGHSTGLSQEVPAMPSLPGTGLTSSLQEELPGTAASLHTNTDVPLPSRDQDFPSSAPT LQLGPGSPTQSHPPEAMATSSEGACAKEPNVDGRSSGTRSCDPGLIDSLKNYLLLLLKLSSPETSEARAE SQEVADTGGLTSSSTLVPTMEVAGLSPRTSRRILERVENNHLVQSAQTLLLSPCTSRRLTGLLDREVQAG QQALAAAQCSRGPCPTPLTIPAIVVGEEGSAGEDSEERTSQESDKKGLLGEVEGHTVESRTQEPCQEEAM PGEALTGLPAATPEELALGARRKRFLPKVRAGSDGEANKAEERESPTVSPRGPRKGLTPGSPGTPGRERR SPTQARKASMLEVPGAEEEPATGDLVSRSKDSGLDSEPAVDEGKQEALAKQRKAKDLLKAPQVIRKIRVE QFPDSSGSLKLWCQFFNIVSDSVLTWAKDQHPVGEVNRSAGDEGPAALAIVQASPTDCGVYRCTIQNEHG SASTDFCLSPEVLSGFISREEGEVGEEIEMTPMVFAKGLADSGCWGDKLFGRLVSEELRGGGHGLQKASR AKVIYGLEPIFESGRTCIIKVSSLLVFGPSSETSLLGRNYDVTIQGCKIQNMSREYCKIFAAEARAASGF GEVPEIIPLYLIYRPANNIPYATLEEDLGKPLQTYCSRQWGCAGAPAAASSSEALQKCQTFQHWLYQWTN GSFLVTDLTGADWKMTDVQIATKLRGYQGLKESCFPALLDQFASSHQCNTYCDMLGLKPLKGPEAAHPQA KAKGSKSPSAGRKGSQLSPQPQKKGLPSPQGSRKSAPSSRATLQASQAATVQLLGQPPVQDGSSKAQSMR SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_054085
ORF Size	5040 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_054085.2, NP_473426.2
RefSeq Size	6369 bp
RefSeq ORF	5043 bp
Locus ID	116904
MW	180.6 kDa

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