Prpf8 (NM_138659) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AU019467; D11Bwg0410e; DBF3/PRP8; Prp8; Sfprp8l
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR218624 representing NM_138659 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGAGTGTTTCCTTACCGAGGGCCGGGTAACCCGGTGCCCGGCCCTCTAGCCCCGCTGCCGGATT ATATGTCAGAGGAGAAGCTGCAGGAGAAAGCTCGAAAATGGCAGCAGTTGCAGGCCAAGCGATATGCAGA GAAGCGGAAATTTGGGTTTGTGGATGCTCAGAAGGAAGACATGCCACCAGAACATGTCAGGAAGATCATT CGAGACCATGGAGACATGACCAACAGGAAGTTCCGTCATGACAAAAGGGTCTATTTGGGAGCTCTTAAGT ACATGCCTCATGCAGTCCTCAAGCTCCTTGAGAACATGCCCATGCCTTGGGAGCAGATCCGGGACGTGCC TGTGCTGTACCACATCACTGGAGCCATCTCCTTTGTTAACGAGATTCCCTGGGTTATCGAGCCGGTTTAC ATCTCCCAGTGGGGGTCAATGTGGATCATGATGCGTAGAGAAAAAAGAGACAGGAGGCATTTCAAGAGGA TGCGTTTCCCCCCTTTCGATGACGAGGAACCGCCCCTGGATTATGCCGACAACATCTTAGATGTTGAGCC ACTGGAAGCCATTCAGCTAGAGCTGGACCCAGAGGAGGATGCCCCTGTGTTGGACTGGTTCTATGACCAC CAGCCTTTGAGAGACAGTAGGAAGTACGTTAATGGTTCCACCTACCAACGCTGGCAGTTCACATTGCCTA TGATGTCCACACTCTACCGACTGGCCAATCAGCTTCTGACTGACCTTGTAGATGACAACTACTTCTATCT CTTTGATCTGAAGGCCTTTTTTACCTCTAAGGCACTCAACATGGCCATTCCTGGGGGACCCAAATTTGAG CCTCTTGTTCGAGACATCAACTTACAGGATGAAGACTGGAATGAATTTAACGATATTAATAAGATCATCA TCCGTCAGCCTATTCGTACTGAGTACAAGATTGCTTTTCCTTACCTGTATAACAACCTTCCACACCACGT CCACCTGACCTGGTACCACACTCCCAACGTTGTGTTCATCAAGACTGAGGATCCTGATCTGCCAGCTTTC TACTTTGATCCTTTGATCAACCCAATCTCCCATAGACACTCAGTCAAGAGCCAAGAACCACTGCCTGATG ATGATGAAGAGTTTGAACTCCCAGAGTTTGTCGAGCCCTTCCTGAAGGATACACCTCTCTATACAGACAA CACAGCCAATGGCATTGCCCTGCTCTGGGCACCACGGCCCTTCAACCTACGCTCTGGTCGTACCCGCCGG GCCTTGGACATACCCCTTGTCAAGAACTGGTATCGGGAGCACTGTCCTGCTGGGCAGCCTGTGAAGGTTC GAGTCTCTTATCAGAAGCTGCTTAAGTACTATGTGCTGAATGCCCTCAAGCACCGGCCTCCCAAAGCCCA GAAGAAGAGGTATCTGTTCCGTTCTTTCAAAGCTACCAAATTCTTTCAGTCCACCAAGCTGGACTGGGTG GAGGTAGGGCTGCAGGTTTGCCGCCAGGGCTACAACATGCTCAACCTTCTCATTCACCGGAAGAACCTCA ACTATCTGCACTTGGACTACAACTTCAACCTGAAGCCTGTCAAGACGCTCACCACCAAGGAAAGAAAGAA ATCCCGTTTTGGGAATGCTTTCCATCTATGTCGGGAAGTACTGCGTTTAACCAAGCTGGTTGTGGACAGC CATGTGCAGTATCGCTTGGGCAATGTAGATGCCTTCCAGCTGGCAGATGGTTTGCAGTATATATTTGCCC ACGTGGGACAGTTGACAGGCATGTATCGATACAAATATAAGCTGATGCGGCAGATCCGCATGTGCAAGGA CTTGAAACATCTCATCTATTACCGCTTCAACACGGGTCCTGTAGGGAAAGGCCCTGGCTGTGGTTTCTGG GCTGCTGGCTGGCGGGTCTGGCTGTTCTTTATGCGTGGAATTACCCCTCTGCTAGAGCGATGGCTGGGCA ACCTTCTGGCCCGACAGTTTGAAGGCCGTCACTCAAAGGGGGTGGCAAAGACAGTAACGAAGCAGCGGGT GGAGTCGCACTTTGACCTGGAGCTCAGGGCTGCTGTGATGCACGACATTCTGGACATGATGCCTGAAGGC ATCAAGCAGAACAAGGCCCGGACAATTCTGCAGCACCTCAGTGAAGCCTGGCGCTGCTGGAAGGCCAACA TCCCTTGGAAGGTCCCAGGACTGCCAACACCTATAGAGAATATGATTCTTCGGTACGTTAAGGCCAAGGC TGATTGGTGGACCAATACTGCCCACTACAACCGGGAACGGATCCGTCGCGGGGCTACTGTAGACAAGACT GTTTGCAAAAAGAACCTGGGCCGCCTCACTCGCCTGTATCTGAAGGCAGAGCAGGAGCGGCAGCACAATT ACCTGAAGGATGGGCCTTACATCACAGCCGAGGAGGCAGTGGCCGTGTATACCACCACTGTGCACTGGCT GGAGAGCCGGAGATTCTCGCCCATCCCCTTCCCTCCACTCTCCTACAAGCATGATACCAAGCTGCTCATC TTGGCTCTGGAGCGTCTCAAGGAAGCTTATAGTGTGAAGTCTAGGTTAAACCAGTCTCAGAGGGAGGAGC TGGGTCTGATTGAACAAGCCTATGACAACCCCCATGAGGCTTTGTCCCGCATCAAGCGTCACCTCCTCAC ACAGAGAGCCTTTAAGGAAGTGGGCATTGAGTTTATGGATCTCTATAGCCACCTTGTACCAGTGTATGAT GTGGAACCACTGGAGAAAATAACTGATGCTTACCTGGACCAGTACCTGTGGTATGAAGCAGATAAACGCC GTCTTTTCCCACCCTGGATCAAGCCTGCTGACACAGAGCCACCTCCACTGCTTGTTTACAAGTGGTGTCA AGGCATTAATAACCTGCAGGATGTGTGGGAGACGAGTGAGGGCGAGTGCAACGTCATGCTGGAGTCTCGG TTTGAGAAGATGTATGAGAAGATTGACCTGACCCTGCTTAACAGGCTGCTGCGACTCATTGTGGACCACA ACATAGCTGACTACATGACGGCCAAGAACAACGTAGTCATCAACTATAAGGACATGAACCATACAAACTC ATATGGAATCATCAGAGGCCTGCAGTTTGCCTCATTCATTGTACAGTACTATGGCTTGGTGATGGATTTG CTTGTACTGGGATTGCACCGGGCCAGTGAGATGGCTGGGCCTCCTCAGATGCCAAATGACTTTCTCAGTT TCCAGGACATAGCCACTGAGGCTGCACACCCAATCCGACTCTTCTGTAGATACATTGATCGCATCCATAT TTTCTTCAGGTTCACAGCAGATGAGGCTCGGGATCTGATCCAGCGTTACCTGACAGAACACCCAGACCCC AACAATGAAAACATTGTTGGCTACAATAACAAGAAATGCTGGCCCCGAGATGCCCGAATGCGGCTCATGA AGCATGATGTCAACTTGGGTCGGGCAGTGTTCTGGGACATCAAAAACCGGCTGCCACGATCGGTGACTAC AGTACAGTGGGAGAATAGCTTTGTGTCTGTGTACAGTAAAGACAACCCCAACCTGCTATTCAACATGTGT GGCTTTGAGTGCCGCATCCTGCCTAAGTGCCGTACCAGCTATGAAGAGTTCACCCACAAGGACGGGGTCT GGAACCTACAGAATGAGGTTACCAAGGAGCGGACAGCTCAGTGCTTCCTGCGTGTGGATGATGAGTCAAT GCAACGCTTCCATAACCGTGTGCGGCAGATTCTGATGGCCTCTGGCTCCACCACGTTCACCAAGATTGTG AATAAGTGGAACACAGCTCTCATCGGCCTTATGACATATTTTCGAGAGGCTGTGGTGAATACACAGGAAC TCCTGGACTTATTGGTGAAGTGTGAAAACAAGATCCAGACACGTATCAAGATTGGATTGAACTCCAAGAT GCCTAGCCGGTTCCCGCCTGTTGTATTCTACACCCCTAAGGAGCTGGGTGGGCTTGGCATGCTCTCGATG GGCCATGTGCTTATCCCCCAGTCTGACCTCAGGTGGTCCAAGCAGACAGATGTGGGCATCACACACTTCC GTTCAGGAATGAGTCATGAAGAAGACCAACTGATTCCCAACTTGTACCGCTACATACAGCCATGGGAGAG CGAGTTCATTGACTCCCAGCGGGTCTGGGCTGAGTATGCACTCAAGAGACAAGAGGCTATTGCTCAGAAC AGACGCTTGACTTTAGAAGATTTAGAAGATTCATGGGATCGAGGCATTCCCCGGATCAATACCCTCTTCC AGAAAGACCGGCACACATTGGCTTATGATAAAGGCTGGCGTGTCCGAACTGACTTTAAGCAGTACCAGGT TTTGAAGCAGAACCCCTTTTGGTGGACACATCAGCGGCATGATGGCAAGCTTTGGAACCTCAATAACTAC CGCACAGACATGATTCAGGCGCTGGGTGGTGTGGAGGGCATTCTGGAGCACACTCTCTTCAAGGGCACTT ACTTCCCCACCTGGGAGGGTCTGTTCTGGGAGAAAGCCAGTGGCTTTGAGGAGTCCATGAAGTGGAAGAA GCTGACCAATGCTCAGCGATCAGGATTAAACCAGATTCCTAATCGTAGATTTACCCTCTGGTGGTCCCCA ACCATCAATCGAGCCAATGTCTATGTGGGCTTCCAGGTGCAGCTGGACCTGACAGGAATCTTCATGCACG GCAAGATCCCCACACTGAAGATCTCTCTCATCCAGATATTCCGAGCTCACTTGTGGCAGAAGATCCATGA GAGCATCGTGATGGACTTGTGTCAGGTCTTTGACCAGGAACTGGATGCACTGGAAATTGAGACAGTGCAA AAGGAGACAATCCATCCCCGAAAGTCCTATAAGATGAACTCTTCCTGTGCGGATATACTACTCTTTGCCT CCTATAAGTGGAATGTCTCCCGGCCCTCGCTGCTGGCAGACTCCAAGGATGTGATGGACAGCACCACCAC ACAGAAGTATTGGATTGACATCCAATTACGCTGGGGAGACTATGATTCCCATGATATTGAGCGCTATGCC CGGGCCAAGTTCCTGGATTACACCACAGACAACATGAGTATCTACCCTTCGCCCACAGGCGTGCTCATTG CCATTGATCTGGCCTATAATCTACACAGTGCCTATGGAAACTGGTTCCCGGGCAGCAAGCCTCTCATACA GCAGGCCATGGCCAAGATCATGAAGGCAAACCCTGCGTTGTATGTGTTACGTGAACGGATACGCAAAGGA TTACAGCTGTATTCATCTGAGCCCACTGAGCCTTACCTGTCCTCTCAGAACTATGGTGAACTCTTCTCTA ACCAGATTATCTGGTTTGTGGATGACACCAATGTCTATAGAGTAACTATCCACAAGACCTTTGAGGGGAA CTTGACAACTAAACCCATCAATGGGGCCATCTTCATCTTCAATCCACGAACGGGACAGCTGTTCCTCAAG ATAATCCACACATCTGTATGGGCTGGACAGAAGCGTTTGGGTCAGTTGGCCAAGTGGAAGACTGCTGAGG AAGTGGCTGCCCTGATCCGATCTCTGCCTGTGGAGGAACAGCCTAAACAGATCATTGTCACTCGGAAGGG CATGCTGGATCCTCTGGAGGTCCACTTACTGGATTTCCCCAATATTGTGATCAAAGGATCGGAGCTCCAG CTGCCCTTCCAGGCTTGTCTCAAGGTGGAGAAGTTTGGGGATCTCATCCTTAAAGCCACCGAGCCCCAGA TGGTCCTTTTCAACCTCTATGATGACTGGCTCAAGACTATCTCCTCCTACACGGCTTTCTCCCGTCTCAT TCTGATTCTGCGGGCTTTGCATGTGAACAATGATAGAGCAAAGGTGATCCTGAAGCCAGACAAGACTACT GTTACGGAACCCCACCACATCTGGCCCACGCTGACTGACGAGGAATGGATCAAGGTGGAGGTACAGCTCA AGGATCTGATCTTGGCTGACTACGGCAAGAAAAACAACGTGAATGTGGCGTCACTGACACAGTCAGAAAT TCGAGACATCATCCTGGGAATGGAGATCTCGGCGCCATCGCAGCAGCGGCAGCAGATAGCTGAGATTGAA AAGCAGACCAAGGAACAGTCCCAGCTCACTGCTACACAGACTCGAACTGTCAACAAGCACGGTGACGAGA TTATCACTTCAACCACTAGCAACTATGAGACCCAGACCTTCTCATCAAAGACTGAATGGAGAGTCAGGGC CATCTCTGCTGCCAACCTGCATCTGCGGACCAATCATATTTATGTTTCATCTGATGACATTAAGGAGACT GGTTATACGTACATTCTTCCGAAAAACGTGCTTAAGAAGTTCATCTGCATATCTGATCTTCGTGCCCAAA TTGCAGGATACCTGTATGGGGTGAGCCCTCCAGATAACCCTCAGGTGAAGGAGATCCGTTGTATTGTGAT GGTGCCGCAGTGGGGCACGCACCAGACTGTGCACCTGCCCAGCCAGCTGCCCCAGCATGAGTACCTCAAG GAGATGGAACCCTTAGGTTGGATCCACACTCAGCCCAATGAATCCCCCCAGCTATCACCCCAAGATGTCA CCACTCATGCCAAAATCATGGCTGACAACCCATCTTGGGATGGGGAGAAGACTATTATCATCACGTGCAG CTTCACACCAGGCTCCTGCACACTGACAGCCTACAAGCTGACACCCAGTGGGTATGAGTGGGGCCGGCAG AACACAGACAAAGGCAACAACCCCAAAGGCTACCTACCCTCGCACTACGAGAGGGTTCAGATGCTCCTGT CTGACCGATTCCTCGGCTTCTTCATGGTCCCCGCCCAGTCCTCCTGGAACTACAACTTTATGGGTGTTCG ACATGATCCCAACATGAAATACGAGCTGCAGCTGGCAAACCCCAAGGAATTCTACCATGAGGTCCACAGG CCCTCCCACTTCCTCAACTTCGCCCTTCTGCAGGAGGGTGAGGTCTACTCTGCAGACAGAGAGGACCTCT ATGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR218624 representing NM_138659 Red=Cloning site Green=Tags(s) MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKII RDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVY ISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDH QPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFE PLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAF YFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRR ALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWV EVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDS HVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFW AAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEG IKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKT VCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLI LALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYD VEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESR FEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDL LVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDP NNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMC GFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIV NKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSM GHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQN RRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNY RTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSP TINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQ KETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYA RAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKG LQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLK IIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQ LPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTT VTEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIE KQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKET GYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPSQLPQHEYLK EMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQ NTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHR PSHFLNFALLQEGEVYSADREDLYA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_138659
ORF Size	7005 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_138659.2, NP_619600.2
RefSeq Size	7500 bp
RefSeq ORF	7008 bp
Locus ID	192159
UniProt ID	Q99PV0
MW	274.1 kDa
Gene Summary	Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome. Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.[UniProtKB/Swiss-Prot Function]

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