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Plcd4 (NM_148937) Mouse Tagged ORF Clone
CAT＃: MR218890
- TrueORF®
Plcd4 (Myc-DDK-tagged) - Mouse phospholipase C, delta 4 (Plcd4), transcript variant 2
ORF Plasmid: tGFP
"NM_148937" in other vectors (3)
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CNY 5,912.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4921507K24Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218890 representing NM_148937
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACATCTCAGATTCAAGACCTACTAGCCACTGATCAGGACCTGCTGCTGATGCAAGAAGGCACAATGA TGCGCAAGGTGAGGACCAAAAGCTGGAAGAAGCTAAGATACTTCAGACTTCAGAATGACGGCATGACAGT CTGGCATGGAAGTCAACCAGAAAGCATGCCCAAGCCCACTTTTTCGATCTCTGATGTGGAGAGGATACGT AAGGGGCAGGATTCTGAGTTGTTGCGCTATCTGGTGGAGGAGTTTCCCCTGGAGCAAGGGTTCACCGTTG TCTTTCATGGGCGCCGCCCCAACCTGGACCTAGTGGCCAACAGTGTTGAAGAGGCCCAGATTTGGATGCG AGGACTCCAGCTGTTGGTGGATCTTGTTGCCAGCATGGACCACCAGGAGCAAATGGATCAAATGTTGAAT GAGTGGTTCCAGCAAGCAGACAGAAACCAGGATGGCCGGATGAGTTTCAGAGAAGCTCAACGGCTGCTGC TTCTGATGAACGTGGAAATGGATGAAGAGTACGCCTTCAGTCTTTTCCAGGAGGCAGATGTTACCCAGTC TGATGATCTGGGGTCGGAAGAATTTGTACAGTTCTATAAGGCCCTGACTAAGCGCACTGAGATTGAAGAA ATATTTGAGGACTTCTCATCTGACAAACAGAAGCTGACCTTGCTGGAATTTGTGGATTTCCTTAGAAAGG AACAGAAAGAAAAAGACCATGCTCCTGACCTCGCTCTGGAACTCATTGACCGCTATGAGCCTTCTGAGAA TGGCCGATTGCTGCATGTGCTGAGCAAGGATGGCTTCCTCAAGTACCTCTGCTCGAAGGATGGAAACATC TTCAACAGTGACTGCCTCCCTATCTACCAGGATATGACTCAGCCTCTGAGCCACTACTACATTAACTCTT CTCACAACACCTACCTAGTGGGGGACCAGCTTTGCGGCCAGAGCAGCGTTGAAGGATACATACGGGCCTT GAAACGGGGGTGCCGCTGTGTGGAGGTGGACACGTGGGATGGACCTGATGGAGAGCCTGTTGTTTATCAT GGGCATACCCTGACCTCTCGCATCCTATTCAAAGATGTGTTGGCAACTCTCGCGCAATATGCCTTCCAGT CATCAGACTACCCTCTCATCTTGTCTCTGGAAAACCACTGCACCTGGGAACAGCAGAGGACCATGGCACA CCATCTGACCGAGATCCTGGGAGAGCAGCTGCTGAGGAACACCCTAGAGGGATTGCTGGTTGACAGCATG CCCTCGCCAGAGCAGCTTCGGGGGAAGATCTTGGTGAAAGGAAAGAAGTTAAGAACAATTGAAGTTGACA AGGAAGAAGAAGAAGAAGAAGAAGAAGAAGAGCTGGAGAAAGATGAGGGGCCAGATCTGGACCCAGCCTC CCCTGAGTTGGACACTCAACCTCAGCCTGAGACCCAGGGGCAGGCCGCTGGGAACAAGAAGGAGAGGAAG AAGAAGGTTATGAAGTGTCCGATGTCTTGTCTGCTTATCTGTGGTCATGTTATGGCCCAGGCTCCTAGTT CCATTCCTGAGTCCATTCTCTTGTCAAAGCAGTTTCTTCTCCTGAGTTCCACAACCATCATGTGCCCAGA CCTATCTGCCCTGGTTGTCTACTTGAGGACTGTCCCCTTCTGCAGCTTCACTCATTCCAAAGAAAACTAC CACATCTATGACATTTCATCCTTTTCTGAGTCCAAGGCCAAGAACCTCATCAAGGAGGCAGGCAACGAGT TTGTACAGCACAATGCCAGACAGTTATGCCGCGTGTATCCCAGTGGTCTGAGGACAGATTCATCCAACTT TAACCCCCAGGAACACTGGAATGTAGGCTGCCAGATGGTGGCTATGAATATGCAGACTGCAGGAAGTGCA ATGGATATCTGTGATGGGCTCTTCCGCCAGAATGGTGGCTCTGGCTATGTGCTGAAGCCAGAATTCCTGA GAGATACCCAGAGTTCATTCAATCCTGAGAGGCCAATTAGTCTTTATAAGGCCCAGATCCTTGTAGTCCA GGTGATCAGTGGCCAACAACTCCCCAAAGTGGATAAAACCAAAGAGACAACCGTTGTGGATCCACTGGTG AAAGTAGAGCTCTATGGTGTTCCTGAAGACACAAAGGAGCAAGAGACCAGCCATGTGGAGAACAATGGTA TTAATCCATACTGGGGGGAGACTTTCTATTTCCGGCTCCAGGTACCTGAACTTGCTATGTTGCGTTTTGT GGTAAAGGATTATAGCAGGAAATCCCGAAATAACTTTATTGGCCAATACACCCTACCTTGGACCTGTATG AAACAAGGCTACCGACATGTATCCTTGCTCTCCAGAGATGGCACTAGCCTCAATCCAGCTTCCATCTTTG TATACACCTGCATGCAGGAAGACCTGGATATGGATGAGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218890 representing NM_148937
Red=Cloning site Green=Tags(s) MTSQIQDLLATDQDLLLMQEGTMMRKVRTKSWKKLRYFRLQNDGMTVWHGSQPESMPKPTFSISDVERIR KGQDSELLRYLVEEFPLEQGFTVVFHGRRPNLDLVANSVEEAQIWMRGLQLLVDLVASMDHQEQMDQMLN EWFQQADRNQDGRMSFREAQRLLLLMNVEMDEEYAFSLFQEADVTQSDDLGSEEFVQFYKALTKRTEIEE IFEDFSSDKQKLTLLEFVDFLRKEQKEKDHAPDLALELIDRYEPSENGRLLHVLSKDGFLKYLCSKDGNI FNSDCLPIYQDMTQPLSHYYINSSHNTYLVGDQLCGQSSVEGYIRALKRGCRCVEVDTWDGPDGEPVVYH GHTLTSRILFKDVLATLAQYAFQSSDYPLILSLENHCTWEQQRTMAHHLTEILGEQLLRNTLEGLLVDSM PSPEQLRGKILVKGKKLRTIEVDKEEEEEEEEEELEKDEGPDLDPASPELDTQPQPETQGQAAGNKKERK KKVMKCPMSCLLICGHVMAQAPSSIPESILLSKQFLLLSSTTIMCPDLSALVVYLRTVPFCSFTHSKENY HIYDISSFSESKAKNLIKEAGNEFVQHNARQLCRVYPSGLRTDSSNFNPQEHWNVGCQMVAMNMQTAGSA MDICDGLFRQNGGSGYVLKPEFLRDTQSSFNPERPISLYKAQILVVQVISGQQLPKVDKTKETTVVDPLV KVELYGVPEDTKEQETSHVENNGINPYWGETFYFRLQVPELAMLRFVVKDYSRKSRNNFIGQYTLPWTCM KQGYRHVSLLSRDGTSLNPASIFVYTCMQEDLDMDEP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_148937 |
| ORF Size | 2421 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_148937.2, NP_683739.2 |
| RefSeq Size | 2981 bp |
| RefSeq ORF | 2424 bp |
| Locus ID | 18802 |
| UniProt ID | Q8K3R3 |
| MW | 92.7 kDa |
| Gene Summary | Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca(2+) mobilization in the zona pellucida-induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression up-regulates the Erk signaling pathway and proliferation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG218890 | Plcd4 (tGFP-tagged) - Mouse phospholipase C delta 4 (Plcd4) transcript variant 2, (10ug) |
CNY 6,370.00 |
|
| MR218890L3 | Lenti ORF clone of Plcd4 (Myc-DDK-tagged) - Mouse phospholipase C, delta 4 (Plcd4), transcript variant 2 |
CNY 7,700.00 |
|
| MR218890L4 | Lenti ORF clone of Plcd4 (mGFP-tagged) - Mouse phospholipase C, delta 4 (Plcd4), transcript variant 2 |
CNY 7,700.00 |
