Cdk13 (NM_001081058) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2310015O17Rik; Cdc2l5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR219227 representing NM_001081058 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAGTAGCTCGGACACGGCGCTGGGGGGAGGCGGGGGCCTGAGCTGGGCCGAGAAGAAGTTGGAGG AGCGCCGCAAGCGGAGGCGATTCCTGTCCCCTCAGCAGCCGCCGCTGCTGTTGCCGCTCCTGCAGCCGCA GCTCCTGCAACCGCCGCCGCCCCCGCCGCCTCTGCTCTTCCTGGCTGCCCCCGGCGCGGCCGCCGCCGCA GCCGCCGCCGCCGCGGCCTCCTCCTCTTGCTTCAGCCCGGGCCCTCCTCTGGAGGTCAAGCGGCTGGCGA GAGGCAAGAGGCGCCCCGGAGGGCGGCAGAAGCGGCGCCGCGGGCCCCGCGCCGGGCAGGAGGCGGAGAA GCGCCGGGTCTTCTCGCTGCCCCAGCCGCAGCAGGACGGCGGTGGCGGTGCCAGTAGCGGCGGGGGTGTG ACCCCGCTGGTGGAGTACGAGGATGTGAGCTCCCAGTCCGAGCAGGGGCTGCTGCTGGGGGGCGCCAGCG CGGCAACGGCGGCGACGGCTGCCGGGGGAACGGGGGGCAACGGCGGGAGTCCGGCCTCCTCCTCCGGCAC GCAGAGGCGCGCGGAGGGGTCGGAGCGCAGGCCGCGCCGGGACCGCCGCAGCAGCAGCGGCCGCAGCAAG GAGCGCCACCGCGAGCACCGGCGGCGGGACGGGACGCGCAGCGGCAGCGAGGCCTCAAAGGCCCGCAGCC GCCACGGACACAGCGGCGAGGAGCGGGCGGAGGCCGCCAAGAGCGGCAGCAGCAGCAGCAGCGGCGGCCG CCGCAAGAGCGCGTCGGCTACGTCCAGCAGCAGTAGCAGCCGCAAGGACCGGGACCTCAAGGCCCACCGC AGCCGGACTAAGTCGTCCAAGGAACCGCCCTCGGCCTACAAGGAGCCGCCCAAGGCCTACCGGGAGGACA AGAGCGAGCCGAAGGCCTACAGGCGGCGGCAGCGGTCCCTGAGCCCGCTGGGAGGCCGGGACGAAAGCCC GGTGTCCCACAGGGCCTCGCAGAGCCTTCGGAGCCGCAAGTCCCCCAGTCCGGCGGGAGGTGGCAGCAGT CCTTATTCCCGGCGGCTGCCGCGCTCCCCGAGCCCCTATAGCCGGCGCCGCTCGCCCAGCTACAGCCGCC ACAGCTCCTACGAGCGGGGCGGCGACGTATCCCCCAGCCCGTACAGCAGCAGCAGCTGGCGGCGCTCGCG CAGCCCCTACAGCCCAGTACTCAGACGATCTGCCAAATCCCGAAGCAGAAGCCCATATTCATCTAGGCAC TCAAGATCTCGGAGCAGGCACAGATTGTCTAGATCAAGAAGTCGTCATTCAAGCATTTCTCCTAGCACAC TAACTCTGAAGAGTAGCTTGGCAGCTGAATTGAACAAGAATAAAAAGGCACGAGCTGCAGAGGCAGCAAG AGCTGCAGAGGCAGCAAAAGCTGCAGAAGCTGCTAAGGCTGCTGAAGCAGCTGCCAAAGCTGCCAAAGCC TCAAATGCTTCTACACCTACCAAGGGGAACACAGAAACTGGTGCCAGTGTCTCACAGACAAACCATGTGA AGGAAGTCAAAAAACTTAAAACTGAGCATGCACCTTCTCCTTCAAGTGGTGGGACCGTCAAAAGCGACAA AGCAAAAACAAAGCCACCGCTTCAAGTAACAAAGGTAGACAATAATTTGACAGTAGAGAAAGCCACCAAG AAAACAGTTGTTGGGAAGGAGAGTAAACCTGCTGCTACAAAGGAAGAACCAGTTTCCACTAAAGAGAAAA GCAAGCCACTCACACCAAGCACAGGAGCCAAGGAGAAGGAGCAGCATGTGGCTTTAGTGACCTCTACGTT ACCGCCATTACCTTTGCCTCCCATGCTGCCTGAAGATAAAGATGCTGATAGCTTAAGAGGCAACATTTCT GTCAAAGCAGTTAAAAAAGAAGTAGAAAAGAAACTCCGATGTCTGCTTGCTGATTTACCATTGCCCCCTG AGTTACCAGGAGGAGATGATCTTTCCAAGAGTCCAGAGGAGAAGAAAACAGCAGCACAGTTACATAGCAA ACGAAGGCCTAAAATATGTGGGCCTCGCTATGGTGAAATCAAAGAAAAAGATATTGACTGGGGGAAACGC TGCGTGGATAAATTTGATATCATCGGAATTATTGGAGAAGGTACTTATGGACAAGTTTACAAAGCCAGGG ACAAAGACACGGGAGAAATGGTAGCCTTAAAGAAAGTACGTCTGGATAATGAAAAGGAGGGTTTCCCAAT TACAGCAATTAGAGAAATTAAAATTCTTCGGCAACTCACCCACCAGAGTATCATCAATATGAAGGAAATC GTGACTGATAAAGAAGATGCTTTGGATTTTAAGAAAGACAAAGGTGCATTTTACCTGGTGTTTGAATATA TGGACCATGATCTGATGGGACTGCTGGAATCAGGCTTGGTTCATTTTAATGAAAACCATATAAAATCTTT TATGAGACAGCTCATGGAAGGCCTGGATTATTGTCATAAGAAGAACTTTTTGCATAGAGATATTAAATGT TCAAATATCCTTCTAAATAATAGAGGACAGATAAAACTTGCAGATTTTGGACTTGCTCGTTTATATAGCT CAGAAGAAAGTCGCCCATATACTAACAAGGTCATTACTTTGTGGTATCGTCCACCTGAATTGCTCTTGGG AGAAGAACGATATACACCAGCCATTGATGTATGGAGCTGTGGATGTATCCTTGGTGAACTCTTCACTAAA AAACCTATATTTCAAGCAAACCAGGAACTTGCACAGCTAGAGCTAATAAGCCGTATATGTGGGAGTCCAT GTCCTGCAGTGTGGCCTGATGTAATCAAACTGCCATATTTCAACACCATGAAACCAAAGAAGCAATATCG GCGGAAGTTAAGAGAAGAATTTGTTTTCATCCCCGCAGCTGCACTCGACTTATTCGATTACATGCTTGCC TTGGATCCCAGTAAGCGCTGCACTGCTGAGCAGGCTCTTCAGTGTGAGTTCCTGCGAGACGTGGAACCCT CCAAAATGCCTCCACCAGACCTTCCTTTGTGGCAAGATTGTCATGAATTATGGAGTAAAAAGAGAAGAAG ACAGAAACAGATGGGCATGACTGATGATCTTTCCACAATCAAAGCCCCTAGGAAGGACCTGTCTCTGGGC TTAGATGACAGCAGAACTAACACACCCCAGGGTGTGCTGCCACCCGCACAGTTGAAATCTCAGAGCAACT CAAATGTAGCACCTGTAATAACAGGCCCTGGACAGCCGTTAAACCACAGTGAATTGGCAATTCTTCTAAA CCTACTACAATCTAAATCAAGTGTTAATATGGCTGATTTTGTCCAAGTGTTGAACATTAAGGTAAACTCT GAGACTCAACAGCAGCTAAATAAAATAAACCTTCCTGCTGGAATTTTGGCAACAGGTGAAAAACAGACAG ATCCATCAACACCACAACAGGAGTCTTCAAAATCATTGGGAGGAGTTCAGCCTTCACAGACCATCCAGCC TAAAGTGGAAACTGATGCTGCCCAGGCTGCTGTGCAGAGTGCATTTGCAGTCCTCTTGACTCAGTTAATA AAGGCCCAACAGTCCAAACAGAAAGATGCCATGCTAGAGGAAAGGGAAAATGGATCAGGACATGAAGCTC CATTGCAACTCAGGCCTCCTCTAGAACCGAGCACTCCTGGATCCGGGCAAGATGACCTCATCCAGCACCA AGACAGGAGGATATTGGAGCTGACACCAGAACCAGACCGGCCTCGAATTCTGCCTCCTGATCAACGGCCT CCTGAACCTCCTGAACCACCACCAGTTACTGAGGAGGACCTAGATTATCGGACAGAAAACCAGCATGTTC CTACCACTAGTTCTTCATTAACTGACCCACATGCTGGAGTGAAGGCAGCCCTCTTACAGCTGCTTGCTCA GCATCAGCCCCAGGATGATCCCAAAAGAGAAGGTGGTATCGATTATCCCACAGGAGACACATATGTGCCC AGTTCAGACTATAAGGACAACTTTGGATCTTCTTTCTCTGCCGCTCCTTACGTTAGCAGTGATGGTCTAG GAAGTAGCTCCGCTGCTGCACCATTGGAAGCACGTAGTTTCATTGGAAACTCAGATATTCAGTCTCTGGA TAACTACAGTACTGCTTCATCTCACACTGGTGGTCCACCTCAAACTTCTGCCTTTACTGAGTCGTTTGCC AGTTCAGTAGCTGGATATGGAGACATTTACCTCAATGCTGGTCCCATGTTGTTTAGTGGAGACAAGGACC ATAGATTTGAATATAGCCATGGTCCTATCACAGTCCTCACAAACAGCAATGACCCTTCCACAGGGCCAGA GAGTACTCATCCCTTGCCAGCAAAGATGCACAACTATAACTATGGTGGTAACTTACAGGAAAATCCAGGT GGCCCTAGCCTCATGCATGGACAGACCTGGACTTCTCCTGCCCAAGGACCTGGATATTCACAAGGATACA GGGGACACATTAGCACATCAGCTGGCAGAGGTCGAGGCAGAGGGTTACCATAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR219227 representing NM_001081058 Red=Cloning site Green=Tags(s) MPSSSDTALGGGGGLSWAEKKLEERRKRRRFLSPQQPPLLLPLLQPQLLQPPPPPPPLLFLAAPGAAAAA AAAAAASSSCFSPGPPLEVKRLARGKRRPGGRQKRRRGPRAGQEAEKRRVFSLPQPQQDGGGGASSGGGV TPLVEYEDVSSQSEQGLLLGGASAATAATAAGGTGGNGGSPASSSGTQRRAEGSERRPRRDRRSSSGRSK ERHREHRRRDGTRSGSEASKARSRHGHSGEERAEAAKSGSSSSSGGRRKSASATSSSSSSRKDRDLKAHR SRTKSSKEPPSAYKEPPKAYREDKSEPKAYRRRQRSLSPLGGRDESPVSHRASQSLRSRKSPSPAGGGSS PYSRRLPRSPSPYSRRRSPSYSRHSSYERGGDVSPSPYSSSSWRRSRSPYSPVLRRSAKSRSRSPYSSRH SRSRSRHRLSRSRSRHSSISPSTLTLKSSLAAELNKNKKARAAEAARAAEAAKAAEAAKAAEAAAKAAKA SNASTPTKGNTETGASVSQTNHVKEVKKLKTEHAPSPSSGGTVKSDKAKTKPPLQVTKVDNNLTVEKATK KTVVGKESKPAATKEEPVSTKEKSKPLTPSTGAKEKEQHVALVTSTLPPLPLPPMLPEDKDADSLRGNIS VKAVKKEVEKKLRCLLADLPLPPELPGGDDLSKSPEEKKTAAQLHSKRRPKICGPRYGEIKEKDIDWGKR CVDKFDIIGIIGEGTYGQVYKARDKDTGEMVALKKVRLDNEKEGFPITAIREIKILRQLTHQSIINMKEI VTDKEDALDFKKDKGAFYLVFEYMDHDLMGLLESGLVHFNENHIKSFMRQLMEGLDYCHKKNFLHRDIKC SNILLNNRGQIKLADFGLARLYSSEESRPYTNKVITLWYRPPELLLGEERYTPAIDVWSCGCILGELFTK KPIFQANQELAQLELISRICGSPCPAVWPDVIKLPYFNTMKPKKQYRRKLREEFVFIPAAALDLFDYMLA LDPSKRCTAEQALQCEFLRDVEPSKMPPPDLPLWQDCHELWSKKRRRQKQMGMTDDLSTIKAPRKDLSLG LDDSRTNTPQGVLPPAQLKSQSNSNVAPVITGPGQPLNHSELAILLNLLQSKSSVNMADFVQVLNIKVNS ETQQQLNKINLPAGILATGEKQTDPSTPQQESSKSLGGVQPSQTIQPKVETDAAQAAVQSAFAVLLTQLI KAQQSKQKDAMLEERENGSGHEAPLQLRPPLEPSTPGSGQDDLIQHQDRRILELTPEPDRPRILPPDQRP PEPPEPPPVTEEDLDYRTENQHVPTTSSSLTDPHAGVKAALLQLLAQHQPQDDPKREGGIDYPTGDTYVP SSDYKDNFGSSFSAAPYVSSDGLGSSSAAAPLEARSFIGNSDIQSLDNYSTASSHTGGPPQTSAFTESFA SSVAGYGDIYLNAGPMLFSGDKDHRFEYSHGPITVLTNSNDPSTGPESTHPLPAKMHNYNYGGNLQENPG GPSLMHGQTWTSPAQGPGYSQGYRGHISTSAGRGRGRGLPY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001081058
ORF Size	4533 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001081058.2, NP_001074527.1
RefSeq Size	7081 bp
RefSeq ORF	4536 bp
Locus ID	69562
UniProt ID	Q69ZA1
MW	165 kDa
Gene Summary	Cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. Has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. Required for RNA splicing, probably by phosphorylating SRSF1/SF2. Required during hematopoiesis.[UniProtKB/Swiss-Prot Function]

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