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Ppp1r16b (NM_153089) Mouse Tagged ORF Clone
CAT＃: MR219271
- TrueORF®
Ppp1r16b (Myc-DDK-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 16B (Ppp1r16b), transcript variant 2
ORF Plasmid: tGFP
"NM_153089" in other vectors (5)
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CNY 4,240.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ANKRD4; C130078N17Rik; TIMAP; Wdt4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR219271 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCCACGTGGACCTGCTCACAGAGCTGCAGCTGCTGGAGAAGGTGCCTACGCTGGAGCGCCTGC GCGCAGCCCAGAAGCGCCGGGCTCAGCAGCTGAAGAAGTGGGCACAGTACGAGCAGGACCTGCTTCATCG CAAGCGCAAGCATGAGCGGAAGCGCAGCACGGGTGGACGTCGCAAGAAGGTGTCCTTCGAGGCCAGTGTA GCGTTGCTGGAAGCCTCACTGAGGAATGACGCCGAAGAAGTTCGCTACTTCCTGAAGAACAAGGTTAGCC CTGATTTGTGCAATGAGGATGGACTTACAGCCTTGCACCAGTGCTGCATTGACAACTTTGAAGAGATTGT CAAGCTGCTGCTGTCCCACGGTGCGAACGTGAATGCCAAGGACAACGAGCTGTGGACGCCCTTGCACGCG GCAGCCACCTGCGGCCACATCAACCTGGTGAAGATCCTCGTTCAGTATGGGGCTGACTTGCTTGCTGTCA ACTCTGATGGAAACATGCCATATGACCTCTGCGAGGATGAACCCACCCTGGATGTCATCGAGACCTGCAT GGCATACCAAGGCATCACACAGGAGAAAATCAACGAGATGCGAGCAGCCCCTGAGCAGAAGATGATTTCA GACATCCACTGCATGATAGCAGCGGGCCAGGATCTCGACTGGATCGATGGCCAGGGTGCCACACTGCTAC ACATAGCTGGAGCCAATGGATATCTGCGAGCAGCCGAGCTCCTCCTGGACCATGGAGTGCGTGTGGATGT GAAGGACTGGGATGGCTGGGAGCCTCTGCATGCAGCAGCCTTCTGGGGACAGATGCCAATGGCAGAGCTG CTGGTGTCCCATGGAGCTAGTCTCAGTGCCAGGACATCCATGGATGAGATGCCAATAGACCTGTGTGAGG AGGAGGAATTCAAGGTCCTGCTGTTGGAGCTGAAACACAAGCATGATGTGATCATGAAGTCGCAGCTGAG GCACAAGTCGTCCCTGAGCCGGAGGACATCCAGCGCGGGCAGCCGTGGGAAGGTGGTGCGTCGAGCCAGC CTGTCAGACAGAACCAATCTATACCGGAAAGAATATGAGGGCGAGGCCATCTTGTGGCAGCAGCGGAGTG CAGCTGAGGACCAGCGGACTTCAACCTACAATGGAGACATCAGGGAGACCAGGACAGACCAGGAGAACAA GGACCCGAACCCCAGGCTGGAGAAGCCCGTGCTGCTCTCAGAATTCTCTACCAAGATCTCACGGGGAGAA CTGGATGGGCCTGTGGAGAATGGCCTCCGGGCTCCTGTCAGCACTTACCAGTATGCGCTGGCCAATGGGG ACATATGGAAGATGCACGAGATGCCTGACTACAGCATGGCCTACGGCAACCCTGGCGTGGCTGATGTCCC CCCACCCTGGAGTGGCTTCAAGGAGCAGAGCCCCCAAACGCTCCTGGAGCTGAAGAGGCAGAGGGCCGCA GCCAAGCTGCTCAGTCACCCATTCTTAAGCACCCACCTGGGCAGCAGCGTGGCCAGGTCGGGGGAGAGCA GCAGCGAAGGCAAGGCCCCCTTGATTGGAGGCAGAACTTCCCCCTACAGCAGCAACGGGACCTCGGTGTA TTATACAGTCACCAGCGGAGATCCCCCGCTCTTGAAGTTCAAGGCCCCCATGGAGGAGATGGAGGAGAAA GTTCACGGCTGCTGCCGGATCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR219271 protein sequence
Red=Cloning site Green=Tags(s) MASHVDLLTELQLLEKVPTLERLRAAQKRRAQQLKKWAQYEQDLLHRKRKHERKRSTGGRRKKVSFEASV ALLEASLRNDAEEVRYFLKNKVSPDLCNEDGLTALHQCCIDNFEEIVKLLLSHGANVNAKDNELWTPLHA AATCGHINLVKILVQYGADLLAVNSDGNMPYDLCEDEPTLDVIETCMAYQGITQEKINEMRAAPEQKMIS DIHCMIAAGQDLDWIDGQGATLLHIAGANGYLRAAELLLDHGVRVDVKDWDGWEPLHAAAFWGQMPMAEL LVSHGASLSARTSMDEMPIDLCEEEEFKVLLLELKHKHDVIMKSQLRHKSSLSRRTSSAGSRGKVVRRAS LSDRTNLYRKEYEGEAILWQQRSAAEDQRTSTYNGDIRETRTDQENKDPNPRLEKPVLLSEFSTKISRGE LDGPVENGLRAPVSTYQYALANGDIWKMHEMPDYSMAYGNPGVADVPPPWSGFKEQSPQTLLELKRQRAA AKLLSHPFLSTHLGSSVARSGESSSEGKAPLIGGRTSPYSSNGTSVYYTVTSGDPPLLKFKAPMEEMEEK VHGCCRIS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153089 |
| ORF Size | 1707 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153089.1, NM_153089.2, NM_153089.3, NM_153089.4, NP_694729.1 |
| RefSeq Size | 6370 bp |
| RefSeq ORF | 1707 bp |
| Locus ID | 228852 |
| UniProt ID | Q8VHQ3 |
| MW | 63.6 kDa |
| Gene Summary | Regulator of protein phosphatase 1 (PP1) that acts as a positive regulator of pulmonary endothelial cell (EC) barrier function. Protects the endothelial barrier from lipopolysaccharide (LPS)-induced vascular leakage (PubMed:21907835). Involved in the regulation of the PI3K/AKT signaling pathway (By similarity). Involved in the regulation of angiogenesis and endothelial cell proliferation through the control of ECE1 dephosphorylation, trafficking and activity (By similarity). Involved in the regulation of endothelial cell filopodia extension (By similarity). May be a downstream target for TGF-beta1 signaling cascade in endothelial cells (By similarity). Involved in PKA-mediated moesin dephosphorylation which is important in EC barrier protection against thrombin stimulation. Promotes the interaction of PPP1CA with RPSA/LAMR1 and in turn facilitates the dephosphorylation of RPSA/LAMR1 (By similarity). Involved in the dephosphorylation of EEF1A1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219120 | Ppp1r16b (untagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 16B (Ppp1r16b), transcript variant 2, (10ug) |
CNY 7,220.00 |
|
| MG208959 | Ppp1r16b (tGFP-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 16B (Ppp1r16b) |
CNY 5,040.00 |
|
| MG219271 | Ppp1r16b (tGFP-tagged) - Mouse protein phosphatase 1 regulatory (inhibitor) subunit 16B (Ppp1r16b) transcript variant 2, (10ug) |
CNY 5,040.00 |
|
| MR219271L3 | Lenti ORF clone of Ppp1r16b (Myc-DDK-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 16B (Ppp1r16b), transcript variant 2 |
CNY 6,460.00 |
|
| MR219271L4 | Lenti ORF clone of Ppp1r16b (mGFP-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 16B (Ppp1r16b), transcript variant 2 |
CNY 6,460.00 |
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