Sidt1 (NM_001159419) Mouse Tagged ORF Clone

CAT＃: MR219344

  • TrueORF®

Sidt1 (Myc-DDK-tagged) - Mouse SID1 transmembrane family, member 1 (Sidt1), transcript variant 1

ORF Plasmid: DDK tGFP

Datasheet
SDS
Plasmid Map

  "NM_001159419" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 6,096.00

CNY 7,320.00

货期*
现货
规格
    • 10 ug
Cited in 1 publication.

Product images

经常一起买 (4)
PS100001
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车
TA592569S
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

添加到购物车

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms AW045928; B830021E24Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR219344 representing NM_001159419
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGGACTGCCTGCGCCTGGCGCTGCTCTGCGCGCTGCCCTGGCTCCTGCGGGCGGCGGTCCCTGGCC
ACCAGGAAGAGCCCCTCGCGAAATCCGCAGAGCTGCGGCGCGATCCGCGAGACCCCGCCAGGGGAGCGGA
TTTCGATCGCGTCTACAGCGGGGTGGTGAGCCTCAGCACCGAGAACATCTACTCCTTCAACCACACCAGC
CATCCTGGCCAGGTGACAGCCGTGCGGGTGCATGTCAACAGTTCCTCTGACAACCTGGACTACCCAGTCC
TGGTTGTGGTTCGCCAGCAGAAAGAAGTGTTGTCCTGGCAGGTCCCTCTGCTCTTCCAAGGACTATACCA
AAGGAGCTACAACTACCAGGAAGTCAGCCGCACTCTATGCCCTTCAAAAGCAACCAATGAGACGGGCCCT
TTGGAGCAGCTGATCTTTGTAGATGTAGCGTCCATGGCGCCCCATGGTGCCCACTACAAGTTGCTGGTCA
CCAAAATCAAGCACTTCCAGCTCCCGACGAATGTTGCCTTCTACTTCACTGCCAGCCCCTCTCAACCTCA
GTATTTTCTATACAAATTTCCTGAAGACGTGGACTCCGTTATCATTAAAGTGGTGTCTGAGAAGGCTTAC
CCGTGTTCAGTTGTCTCCGTTCAGAACATCATGTGTCCAGTGTATGATCTGGACCACAATGTGGAATTCA
ATGGTGTCTATCAGTCCATGACCAAGAAAGCCGCCATCACGCTGCAGAAGAAGGATTTTCCAGATGAGCA
GTTCTTCGTGGTGTTTGTGATAAAACCTGAAGATTATGCCTGTGGCGGATCATTCTCCATCCAGGAAAAT
GAAAACCAGACCTGGAATCTACAACGATCAAAGAACCTGAAAGTGACCATTGTCCCATCCATCAAAGAAT
CTGTATATGTGAAATCTAGTCTTTTCAGCATATTCGTTTTCTTGTCCTTCTACTTGGGATGCCTGCTCGT
TGTGCTTGTCCATCATGTGAGGTTTCAGAGGAAATCTATTGACGGAAGCTTTGGTTCCAGCGATGGCTCT
GGAAATATGGCTGTCTCACATCCCATCACTGCCAGCACGCCCGAAGGGAGCAATTATGGGGCAATAGATG
AGTCCAGTTCCAGTCCTGGGAGGCAGATGTCTTCTTCCGATGGTGGGCAGCCTTGCCACTCAGACACGGA
CAGCTCTGTGGAGGAGAGTGACTTTGATACCATGCCTGACATCGAAAGCGACAAAAACGTCATCCGGACC
AAGATGTTCTTGTACCTGTCAGATCTGTCCAGGAAGGACCGGAGGATTGTCAGCAAAAAGTATAAGATTT
ATTTTTGGAACATCATCACCATCGCTGTGTTTTATGCACTGCCTGTGATGCAGCTTGTTATCACCTACCA
GACAGTGGTGAATGTCACTGGCAACCAGGATATCTGTTACTACAACTTCCTTTGTGCTCACCCCCTGGGT
GTCCTGAGCGCCTTCAATAATATTCTCAGTAACCTGGGCCACGTACTCCTGGGCTTCCTCTTCCTGCTGA
TAGTTCTGCGCAGAGACCTTCTCCACCGAAGAGCCCTGGAAGCTAAAGACATCTTTGCTATGGAATACGG
TATTCCCAAACACTTTGGTCTCTTCTACGCTATGGGGATAGCACTGATGATGGAGGGCGTGCTCAGTGCT
TGTTACCATGTCTGCCCTAATTACTCCAACTTCCAGTTCGACACTTCCTTCATGTACATGATCGCTGGCC
TCTGCATGCTGAAGCTCTATCAGACCCGCCACCCGGACATCAACGCCAGCGCCTACTCTGCTTATGCCTC
CTTCGCTGTGGTCATCACACTGACGGTCCTCGGAGTGGTGTTTGGAAAAAACGATGTGTGGTTCTGGATC
ATCTTCTCTGCCATCCACATTCTCTCTTCTCTGGCCCTCAGCACCCAAATCTACTACATGGGCCGTTTCA
AGATAGATGTGTCTGACACAGATCTGGGAATCTTCCGGCGGGCTGCTATGGTGTTCTACACGGACTGTAT
CCAGCAATGCAGCAGACCTCTGTATATGGACAGGATGGTGTTGCTCATTGTGGGAAATCTGGTTAATTGG
TCCTTTGCCTTCTTTGGATTGATCTACCGGCCCAGGGACTTTGCCTCCTACATGCTGGGCATCTTCATCT
GCAACCTGCTGCTGTACTTGGCTTTCTACATCATCATGAAGCTTCGAAGCTCTGAGAAAGTCCTCCCGCT
ACCAGTCTTCTGCATCGCGGCCACTGCCGTGGTGTGGGCTGCGGCACTGTACTTCTTCTTCCAGAACCTC
AGCAGCTGGGAGGGGACCCCTGCAGAATCCCGGGAGAAGAACCGTGAGTGTGTCCTGCTGGATTTCTTTG
ATGACCATGACATCTGGCACTTCCTCTCTGCGACTGCCCTGTTTTTCTCATTCCTGGTGCTGTTGACCCT
GGATGATGACCTGGATGTGGTCCGGAGAGACCAGATCCCTGTCTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>MR219344 representing NM_001159419
Red=Cloning site Green=Tags(s)

MLDCLRLALLCALPWLLRAAVPGHQEEPLAKSAELRRDPRDPARGADFDRVYSGVVSLSTENIYSFNHTS
HPGQVTAVRVHVNSSSDNLDYPVLVVVRQQKEVLSWQVPLLFQGLYQRSYNYQEVSRTLCPSKATNETGP
LEQLIFVDVASMAPHGAHYKLLVTKIKHFQLPTNVAFYFTASPSQPQYFLYKFPEDVDSVIIKVVSEKAY
PCSVVSVQNIMCPVYDLDHNVEFNGVYQSMTKKAAITLQKKDFPDEQFFVVFVIKPEDYACGGSFSIQEN
ENQTWNLQRSKNLKVTIVPSIKESVYVKSSLFSIFVFLSFYLGCLLVVLVHHVRFQRKSIDGSFGSSDGS
GNMAVSHPITASTPEGSNYGAIDESSSSPGRQMSSSDGGQPCHSDTDSSVEESDFDTMPDIESDKNVIRT
KMFLYLSDLSRKDRRIVSKKYKIYFWNIITIAVFYALPVMQLVITYQTVVNVTGNQDICYYNFLCAHPLG
VLSAFNNILSNLGHVLLGFLFLLIVLRRDLLHRRALEAKDIFAMEYGIPKHFGLFYAMGIALMMEGVLSA
CYHVCPNYSNFQFDTSFMYMIAGLCMLKLYQTRHPDINASAYSAYASFAVVITLTVLGVVFGKNDVWFWI
IFSAIHILSSLALSTQIYYMGRFKIDVSDTDLGIFRRAAMVFYTDCIQQCSRPLYMDRMVLLIVGNLVNW
SFAFFGLIYRPRDFASYMLGIFICNLLLYLAFYIIMKLRSSEKVLPLPVFCIAATAVVWAAALYFFFQNL
SSWEGTPAESREKNRECVLLDFFDDHDIWHFLSATALFFSFLVLLTLDDDLDVVRRDQIPVF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001159419
ORF Size 2496 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001159419.1, NP_001152891.1
RefSeq Size 4281 bp
RefSeq ORF 2499 bp
Locus ID 320007
UniProt ID Q6AXF6
MW 94.9 kDa
Gene Summary In vitro binds long double-stranded RNA (dsRNA) (500 and 700 base pairs), but not dsRNA shorter than 300 bp (PubMed:26067272). Not involved in RNA autophagy, a process in which RNA is directly imported into lysosomes in an ATP-dependent manner, and degraded (PubMed:27046251).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this cDNA Clones has been cited in the following citations:
Systemic RNA Interference Deficiency-1 (SID-1) extracellular domain selectively binds long doublestrandedRNA and is required for RNA transport by SID-1 ,Li, W;Koutmou, KS;Leahy, DJ;Li, M;, JBC ,PubMed ID 26067272 [SIDT1]

Other Versions

SKU Description Size Price
MG219344 Sidt1 (tGFP-tagged) - Mouse SID1 transmembrane family member 1 (Sidt1) transcript variant 1, (10ug)
    • 10 ug

CNY 8,080.00

MR219344L3 Lenti ORF clone of Sidt1 (Myc-DDK-tagged) - Mouse SID1 transmembrane family, member 1 (Sidt1), transcript variant 1
    • 10 ug

CNY 9,220.00

MR219344L4 Lenti ORF clone of Sidt1 (mGFP-tagged) - Mouse SID1 transmembrane family, member 1 (Sidt1), transcript variant 1
    • 10 ug

CNY 9,220.00

You may also need
plasmid icon Empty Vector
protein iconDDK/Flag Antibody

其它Sidt1产品

Expression Plasmids
Lentiviral Particles
CRISPR
RNAi
Proteins
Antibodies
相关产品 (8)

PS100001

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【特别福利】购一抗正装产品，免费领10ul抗体试用装

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MR219344L4V

Lenti ORF particles, Sidt1 (GFP-tagged) - Mouse SID1 transmembrane family, member 1 (Sidt1), transcript variant 1, 200ul, >10^7 TU/mL

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MG219344

Sidt1 (tGFP-tagged) - Mouse SID1 transmembrane family member 1 (Sidt1) transcript variant 1, (10ug)

    • 10 ug

CNY 8,080.00

MR219344L3V

Lenti ORF particles, Sidt1 (Myc-DDK-tagged) - Mouse SID1 transmembrane family, member 1 (Sidt1), transcript variant 1, 200ul, >10^7 TU/mL

    • 200 ul

CNY 13,300.00 暂停销售

MR219344L4

Lenti ORF clone of Sidt1 (mGFP-tagged) - Mouse SID1 transmembrane family, member 1 (Sidt1), transcript variant 1

    • 10 ug

CNY 9,220.00

MR219344L3

Lenti ORF clone of Sidt1 (Myc-DDK-tagged) - Mouse SID1 transmembrane family, member 1 (Sidt1), transcript variant 1

    • 10 ug

CNY 9,220.00

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