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Tbc1d8b (NM_001081499) Mouse Tagged ORF Clone
CAT＃: MR219347
- TrueORF®
Tbc1d8b (Myc-DDK-tagged) - Mouse TBC1 domain family, member 8B (Tbc1d8b)
ORF Plasmid: tGFP
"NM_001081499" in other vectors (4)
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CNY 11,632.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4921505D17Rik; 9030605E16Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR219347 representing NM_001081499
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCTGAAGCCCGAGGAAGTGCTGTTGAAAAATGCGCTGAAGCTGTGGCTGATGGAAAGATCCAACG AGTACTTTGTCCTGCAAAGGCGTCGAGGCTACGGGGAAGAAGGAGGAGGGGGACTTACAGGGCTCTTGGT TGGGACTCTTGATTCTGTCTTAGACTCCACTGCTAAAGTAGCTCCATTTCGCATCCTACACCAGACACCA GATTCTCAAGTGTACTTGTCAATTGCATGTGGAGCCAACAGAGAAGAAATAACAAAACATTGGGACTGGT TGGAACAAAATATAATGAAGACTTTATCTGTGTTTGATTCAAATGAAGATATTACCAATTTTGTACAAGG CAAAATAAGAGGACTAATCGCTGAAGAGGGGAAACAGTCTTTTGCTAAAGAAGATGATCCTGAGAAATTT CGAGAAGCCCTTTTGAAATTTGAAAAATCTTTTGGTTTACCAGAGCAGGAAAAATTAGTAACTTATTATT CCTGCAGTTACTGGAGAGGACGGGTCCCTTGTCAGGGCTGGCTCTATCTAAGTACCAACTTTCTGAGCTT CTATTCCTTCTTGCTAGGATCAGAAATTAAACTCATTATCTCCTGGGATGCAATCTCAAAACTTGAAAAG ACTTCAACTGTTATATTAACTGAGAGCATTCACGTATGTTCTCAAGGAGAGAATCACTATTTTTCAATGT TTTTGCACATTAATGAGACATACCTTCTTATGGAACAACTGGCAAACTATGCCATAAAGAGACTTTTTGA TAAAGAAACATTTGATAATGACCCAGTCCTTGATGATCCTCTACAGATTACCAAAAGAGGTTTGGAATAT CGAGCCCACAGTGAGCAATTCAAAGCCTTTTTTAGGCTACCCAAAGAAGAGACATTGAAAGAAGTACATG AGTGTTTCTTATGGGTACCATTCAGCCACTTCAGTAGTCACGGAAAAATGTGCATTTCAGAAAACTATAT TTGCTTTGCTAGCCAGGATGGGAATCTATGCAGTGTAATCATTCCATTACGAGAGGTCTTAGCTATAGAT AAAACTGATGACTCCAACAGATCGGTCATCATTAGCATCAAAGGAAAAACAGCTTTTCGTTTCAGTGAGC TTAAAGACTTTGAGCAACTGGTGGCAAAACTCAGGCTCAAGTGCCGGGCAGCTTCAACTCAAGATGATGT TAGTACAGAGGTTGCTGTTAGTTCTGACTCGACAGGCCCATCTGAGAATTTTGAGGAGCAGCCTTTGACA TGTCCAAAGGAATGCAGCAAAACTGTGAACACTGAAGCCTTAATGACAGTATTTCACCCTCAAAATCTGG AAAATCTTGATTCTAAAATGTTGAAAGAAAAGATGAAGGAGCAATCATGGAACATCTTATTTTCAGAATG TGGGCGAGGTGTTAGCATGTTCCGGACAAAAAAGACTCGAGATCTGGTGGTAAGAGGGATTCCAGAGACG TTAAGAGGAGAGCTCTGGATGCTTTTCTCAGGTGCTGTTAATGACATGGCAACAAACCCTGGCTATTATG CTGAAGTGGTTGAGCAATCCTTAGGGACCTCCAACTTGGCTACTGAAGAAATTGAGCGTGATTTGCGTCG CTCTCTGCCCGAACATCCAGCATTTCAGAGTGATACTGGCATATCTGCTCTGAGGCGGGTACTGACAGCT TATGCATATAGGAATCCCAAAATAGGATACTGCCAGGCAATGAACATTTTGACTTCAGTATTGCTCCTAT ATGCAAAAGAAGAAGAGGCTTTTTGGCTTCTGGTTGCTGTATGTGAACGAATGTTGCCTGATTATTTTAA TCGTCGAATCATTGGTGCCTTGGTGGACCAGGCAGTGTTTGAAGAACTTATCAGGGATCACCTTCCTCAG CTGACAGACCACATGACCGACATGACTTTCTTTTCCTCTGTTTCTCTTTCCTGGTTTCTCACACTTTTCA TTAGTGTGCTACCTATTGAAAGTGCAGTGAATGTGGTGGACTGTTTCTTCTATGATGGAATAAAAGCCAT TTTACAACTGGGATTGGCAATACTTGACTATAATTTAGACAAACTTCTGACATGTAAAGATGATGCTGAA GCGGTGACGGCACTAAACAGGTTCTTTGACAATGTCATTAATAAGGATAGCCCACTGCCTTCAAATGTTC AACAGGGTTCAAATATCAGTAATGAAAAAAGTGATCATACTAAAGTGGATATTACAGATTTGATTAAAGA GTCCAATGAGAAATATGGTAGTATCCGCTATGAAGATATACATAGCATGCGCTGTCGAAATAGGTTGTAT GTGATCCAGACCCTGGAAGAGACAACAAAGCAGAATGTGCTACGTGTTGTATCGCAAGACGTGAAAATGA GCCTTCAGGAACTGGACGAACTTTATGTCATTTTTAAGAAAGAGCTGTTTATATCTTGTTATTGGTATTT GAGTTGTCCAGGATTGAAGCATCATGACCCCAGCCTGCCATATTTGGAACAATATCAGATTGACTGCCAG CAATTCAGAGTATTATATCACTTGCTGAGTCCCTGGGCTCATTCTGCAAACAGAGACTCATTGGCTTTAT GGACATTCCGATTGTTGGATGAGAACTCTGATTGCCTTATAAACTTCAAAGAATTCTCCTCTGCAATAGA TATAATGTACAATGGAAGTTTTACTGACAAGCTCAAGCTGCTTTTTAAGCTGCATATTCCTCCAGCTTAT ACTGAAGTGATGTCTAAGACGTCTTCAAAGGGCGATGAGCTTTCAACAGAAGAATTACTTTATTTCAGTC AGCTCCAGGTTTCTAAGCCTGCAGATGAGAAGGAAACAGAATCGGGAAGAAATAGCCCTGAAAAAGGCAA AGGAAAAATTGATATTCAAGCCTATTTAAGTCAATGGCAAGATGAGCTTCTTAAAAAAGAAGAAACCATT AAAGATTTACCAAGAATGAATCAGTCACAATTTATTCAATTTTCAAAGACCCTGTATAATTTATTTCATG AGGACCCTGAAGAAGAGTCGCTATATCAAGCCATTGCTATTGTAACCAACCTTCTGCTCAGGATGGAAGA AGTTGGAAGGAAACTCCATAGCCCGGCATCATCAGCCAGTACAGCTCGTGATTCTGGACCCAGTGAAGGG AATGCAGAGAGCAGTGTGAAGAAAGATCTTCCCTCTCCCAGGGAAGAACATCAGTGGTCGTTTGCATTTG AGCAGATTCTTGCTTCCCTGTTAAATGAACCAGCCTTGGTGAGGTTTTTTGAGAGACCCTTAGATTTAAA AGCCAAGCTAGAAAATGCGAAAAGCTCCCAGCTAAGGTCTAGAACCAAGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR219347 representing NM_001081499
Red=Cloning site Green=Tags(s) MWLKPEEVLLKNALKLWLMERSNEYFVLQRRRGYGEEGGGGLTGLLVGTLDSVLDSTAKVAPFRILHQTP DSQVYLSIACGANREEITKHWDWLEQNIMKTLSVFDSNEDITNFVQGKIRGLIAEEGKQSFAKEDDPEKF REALLKFEKSFGLPEQEKLVTYYSCSYWRGRVPCQGWLYLSTNFLSFYSFLLGSEIKLIISWDAISKLEK TSTVILTESIHVCSQGENHYFSMFLHINETYLLMEQLANYAIKRLFDKETFDNDPVLDDPLQITKRGLEY RAHSEQFKAFFRLPKEETLKEVHECFLWVPFSHFSSHGKMCISENYICFASQDGNLCSVIIPLREVLAID KTDDSNRSVIISIKGKTAFRFSELKDFEQLVAKLRLKCRAASTQDDVSTEVAVSSDSTGPSENFEEQPLT CPKECSKTVNTEALMTVFHPQNLENLDSKMLKEKMKEQSWNILFSECGRGVSMFRTKKTRDLVVRGIPET LRGELWMLFSGAVNDMATNPGYYAEVVEQSLGTSNLATEEIERDLRRSLPEHPAFQSDTGISALRRVLTA YAYRNPKIGYCQAMNILTSVLLLYAKEEEAFWLLVAVCERMLPDYFNRRIIGALVDQAVFEELIRDHLPQ LTDHMTDMTFFSSVSLSWFLTLFISVLPIESAVNVVDCFFYDGIKAILQLGLAILDYNLDKLLTCKDDAE AVTALNRFFDNVINKDSPLPSNVQQGSNISNEKSDHTKVDITDLIKESNEKYGSIRYEDIHSMRCRNRLY VIQTLEETTKQNVLRVVSQDVKMSLQELDELYVIFKKELFISCYWYLSCPGLKHHDPSLPYLEQYQIDCQ QFRVLYHLLSPWAHSANRDSLALWTFRLLDENSDCLINFKEFSSAIDIMYNGSFTDKLKLLFKLHIPPAY TEVMSKTSSKGDELSTEELLYFSQLQVSKPADEKETESGRNSPEKGKGKIDIQAYLSQWQDELLKKEETI KDLPRMNQSQFIQFSKTLYNLFHEDPEEESLYQAIAIVTNLLLRMEEVGRKLHSPASSASTARDSGPSEG NAESSVKKDLPSPREEHQWSFAFEQILASLLNEPALVRFFERPLDLKAKLENAKSSQLRSRTKM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081499 |
| ORF Size | 3342 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001081499.2, NP_001074968.1 |
| RefSeq Size | 5907 bp |
| RefSeq ORF | 3345 bp |
| Locus ID | 245638 |
| UniProt ID | A3KGB4 |
| MW | 127.9 kDa |
| Gene Summary | May act as a GTPase-activating protein for Rab family protein(s).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223629 | Tbc1d8b (untagged) - Mouse TBC1 domain family, member 8B (Tbc1d8b), (10ug) |
CNY 14,250.00 |
|
| MG219347 | Tbc1d8b (tGFP-tagged) - Mouse TBC1 domain family member 8B (Tbc1d8b), (10ug) |
CNY 12,260.00 |
|
| MR219347L3 | Lenti ORF clone of Tbc1d8b (Myc-DDK-tagged) - Mouse TBC1 domain family, member 8B (Tbc1d8b) |
CNY 13,020.00 |
|
| MR219347L4 | Lenti ORF clone of Tbc1d8b (mGFP-tagged) - Mouse TBC1 domain family, member 8B (Tbc1d8b) |
CNY 13,020.00 |
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