Ascc3 (NM_198007) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ASC1p200; B630009I04Rik; BC023451; D430001L07Rik; D630041L21; Helic1; RNAH
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR219721 representing NM_198007 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCTACCTCGGCTCACAGGAGCCTTGCGCTCCTTTTCAAATGTCACCAAGCAAGATAATTACAATG AAGAAGTAGCTGACTTGAAGTTGAAGCGATCTAAACTTCATGAACAAGTTTTAGATTTTGGCCTTACATG GAAGAAGATAGTGAAATTTTTGAATGAAAAGCTAGAGAAGAATAAAATGCAAAATATAAATGAAGACTTA AAAGACATACTACAAGCTGCAAAACAAATCGTTGGAACGGATAATGGGAGAGAAGCAATTGAGAGTGGGG CTGCATTTCTCTTTATGACATTTCACATGACGGACTCTGTTGGTTACATGGAGACAAAGGCTATCAGACA GACGTTTGGTCCCTTTCCATCGTCATCTGCTACTTCAGCTTGTAATGCCACTAATCGAATTATTTCTCAT TTTAGTCAAGATGATCTCACTGCTTTTGTCCAGATGGCAGAAAACCCATGTAACGATAGAGTGGTTTTTG GCAAAAACTTAGCATTTTCTTTTGACATGTATGATCTGGACCACTTTGATGAATTACCAATAAATGGTGA ATCCCAGAAAACTATAAGCTTAGACTACAAGAAGTTCCTGAATGAACAGTTCCAGGAACCCTACACTCCT GAGCTCAAGCCTGTGGAGAAAACAAATGGCTCCCTATTGTGGTGTGAAGTTGAGAAGTACTTAAACGCAA CGTTAAAGGAAATGACTGAAGCAGCAAGAGTTGAAGATCTTTGCTGTACTCTATATGACATGCTTGCTTC TGCTAAAAGCGGCGATGAACTTCAGGACGAGCTGTTTGAATTGCTGGGACCTGAAGGACTGGATCTGATT GAGAAGCTGCTCCAGAACAGAATTACAATTGTAGATCGGTTCCTTAATTCCTCGAGCGATCATAAGTTTC AAGTTCTCCAAGATAGTTGTAAAAAGATTTTAGGAGAGAATTCAAAGCCTAATTATGGATGTCAAGTCAC TATTCAATCTGAACAAGAAAAACAGTTAATGAAACAATATCGACGTGAAGAAAAAAGAATTGCCAGGCGA GAAAAAAAGGCTGGAGAAGATGGGGAAGTTTCTGGAGAAGGAGTTTTGCCCTTTGATCCTAAGGAACTCC GGATACAGAGAGAACATGCACTTCTGAATGCTAGAAATGCTCCAATTTTAGGAAGACAGAGAGACGTGGA GTTTGAAAAAATCCGTTATCCTCATGTTTATGATTCCCAGGCTCAGGCCCGAGAAACATCAGCATTCATA GCTGGAGCCAAGATGATTTTACCAGAAGGAATCCAGAGAGAGAATACCAAGCTGTATGAGGAAGTCAGGA TCCCCTATGGTGAACCAATGCCAGTTGGGTTTGAGGAAAAACCAGTTTATATCAAAGATTTAGATGAGGT TGGACAGTTGGCTTTTAAAGGAATGAAGAGACTCAATAGAATCCAGTCTATAGTATTTGAAACAGCCTAC AACACCAACGAAAACATGCTGATCTGTGCCCCTACGGGAGCTGGGAAGACCAATATTGCAATGCTTACTA TCTTACATGAAATCCGCCAACACTTTCATCAAGGTGTTATTAAAAAGAATGAGTTTAAGATTGTGTATGT CGCTCCAATGAAAGCCTTGGCAGCTGAGATGACAAATTACTTCAGCAAACGTCTAGAGCCACTGGGCATT GTTGTGAAAGAATTGACTGGAGATATGCAGTTGTCAAAAAGTGAAATTTTACGAACTCAGATGCTGGTGA CCACACCAGAAAAATGGGATGTAGTAACAAGAAAGAGTGTTGGGGATGTTGCTCTTTCCCAAATCGTAAA ACTCCTGATTCTTGACGAGGTGCACTTGCTGCATGAAGACAGAGGCCCAGTGCTAGAAAGCATAGTCGCC AGGACCTTACGGCAGGTTGAATCCACACAGAGTATGATAAGAATTCTTGGATTGTCTGCAACCTTACCCA ACTACCTCGATGTTGCTACATTTTTGCATGTTAACCCCTACATTGGACTTTTCTACTTTGATGGCCGTTT TCGACCTGTACCTCTTGGACAGACATTTTTAGGGATTAAAAGTACAAACAAGATGCAGCAGTTAAATAAC ATGGATGAAGTGTGCTACGAAAGTGTTCTGAAACAAGTAAAGGCTGGACACCAGGTGATGGTATTTGTAC ATGCTCGCAATGCCACTGTAAGGACCGCTATGTCTCTAATAGAGAGAGCAAAAAATAGTGGCCAAATTTC TTGCTTTCTACCCACCGAAGGGCCAGAGTACGGACATGCATTAAAACAGGTACAAAAGTCAAGAAATAAG CAAGTGCGAGAATTATTCTCCGATGGCTTTAGCATTCATCATGCCGGGATGCTTCGGCAAGACAGAAATT TGGTTGAAAATTTGTTTTCAAATGGGCATATCAAAGTCCTAGTCTGTACAGCCACATTAGCCTGGGGTGT CAATCTTCCTGCACATGCCGTGGTTATTAAGGGAACACAAATATACGCTGCCAAAAGAGGCTCCTTTGTT GACCTTGGAATTTTAGATGTCATGCAGATATTTGGCCGAGCTGGACGACCGCAATTTGACAAATTTGGGG AAGGAATCATTATAACAACCCACGATAAACTCAGCCATTACCTCAGTTTGCTCACTCAACAAAACCCAAT TGAGAGTCAGTTTCTGGAAAGTCTTGCAGATAACCTAAATGCAGAGATTGCGCTGGGGACAGTCACCAAC GTGGAAGAGGCAGTGAGGTGGATGAGTTACACTTACCTCTATGTGCGCATGCGAGCAAACCCGCTAGCAT ACGGCATCAGTCACAAAGCGTACCAGATTGACCCAACACTAAGGAAGCATCGAGAACAGCTCCTTATTGA AGTTGGACAAAAGTTGGATAAAGCAAAGATGATTCGTTTTGAAGAACGAACTGGCTATTTTTCTTCTACT GATTTGGGTAGAACTGCCAGCCACTTCTATATTAAATACAACACCATTGAGACATTTAATGAACTCTTTG ATGCACACAAAACAGAGGGTGATATCTTTGCTATTGTTTCCAAAGCTGAAGAATTTGATCAAATTAAGGT CAGAGAAGAAGAAATAGAAGAATTAGATGCCTTGTTAAACAATTTCTGTGAACTCTCAGCTCCAGGAGGT GTGGAAAATAGTTATGGGAAAATAAATATCCTACTTCAAACCTACATCAGCCGAGGAGAGATGGACAGTT TCTCCCTCATATCAGATTCTGCATATGTCGCCCAGAATGCAGCTAGAATTGTCCGTGCTCTCTTTGAAAT TGCACTGAGGAAACGTTGGCCTACCATGACCTACAGGCTTTTGAATCTTAGTAAAGTCATTGACAAGAGA CTCTGGGGTTGGGCTAGCCCTTTGAGACAATTTTCTGTGTTACCACCACACATTCTAACAAGATTAGAAG AAAAAAACCTTACTGTGGATAAACTCAAAGACATGAGAAAAGATGAAATAGGTCACATTTTGCATCATGT GAATATTGGACTGAAGGTCAAACAGTGTGTTCATCAAATTCCTTCTGTTACAATGGAAGCTTCCATTCAG CCCATCACACGGACTGTCCTCCGAGTATCACTGAACATCCACCCTGATTTCTCTTGGAATGACCAGGTTC ATGGAACAGTGGGGGAACCATGGTGGATTTGGGTAGAAGATCCTACAAATGACCATATTTATCACTCGGA GTACTTTCTAGCTCTTAAAAAACAGGTTATTAATAAGGAAGCTCAACTTCTGGTATTTACAATTCCTATT TTTGAGCCTTTGCCCTCTCAGTACTACATTCGAGCAGTGTCTGATCGATGGCTAGGAGCTGAAGCTGTTT GCATTATCAACTTTCAACATCTGATTCTACCAGAGAGACATCCTCCCCATACAGAATTATTGGACCTTCA ACCTTTACCAATTACAGCTTTGGGGTGCAAAGCATATGAAGCTCTATATAACTTCAGCCACTTTAACCCT GTACAGACGCAGATATTCCACACACTGTATCACACAGACTGTAATGTTCTGCTTGGCGCCCCCACCGGAT CAGGAAAGACAGTTGCAGCTGAACTAGCCATTTTCAGAGTTTTCAACAAATATCCTACTTCAAAGGCAGT ATATATTGCACCCCTCAAAGCTCTAGTACGTGAAAGAATGGATGATTGGAAAATTAGGATAGAAGAAAAA CTTGGTAAAAAAGTTATTGAACTAACTGGGGATGTGACTCCAGACATGAAATCCATTGCTAAGGCTGACC TTATTGTCACCACACCAGAAAAGTGGGATGGAGTTAGTCGAAGCTGGCAGAATAGAAGCTATGTTCAGCA GGTCAACATTCTCATCATAGATGAGATCCATCTGCTAGGGGAGGAGAGAGGCCCAGTTTTAGAAGTCATT GTATCTCGAACAAACTTTATCTCATCACACACAGAAAAACCAGTTAGAATAGTTGGACTGTCCACTGCAT TAGCTAATGCTAGAGATCTTGCTGACTGGCTCAACATTAAGCAGATGGGTTTGTTTAACTTCCGACCATC AGTTCGCCCAGTTCCACTGGAAGTTCACATTCAGGGTTTCCCAGGACAACATTACTGCCCACGCATGGCT AGTATGAACAAGCCTGCATTTCAGGCAATTAGAAGTCACTCTCCAGCCAAGCCTGTTTTGATATTTGTCT CATCAAGACGTCAAACACGACTTACAGCCTTAGAGTTGATAGCATTTCTGGCCACTGAAGAAGACCCAAA GCAGTGGTTGAATATGGATGAGCAAGAGATGGACAACATCATTGGAACAGTACGAGATTCCAACCTCAAG CTGACTCTAGCTTTTGGAATAGGAATGCATCATGCTGGCCTGCATGAAAGAGACCGAAAAACTGTAGAAG AGCTGTTTGTGAACTGTAAAGTTCAGGTTCTCATTGCTACAAGCACATTAGCATGGGGTGTAAACTTTCC AGCACACTTAGTAATTATTAAGGGAACTGAATATTATGATGGAAAGACAAGACGTTATGTGGATTTTCCC ATCACAGATGTGCTGCAGATGATGGGACGAGCTGGGAGACCTCAGTTTGATGACCAGGGCAAAGCTGTAA TTCTAGTTCATGATATAAAAAAAGATTTTTATAAAAAGTTTCTTTATGAACCTTTCCCAGTAGAATCAAG CTTATTAGGAGTGTTGTCTGACCACTTAAATGCAGAGATTGCTGGAGGTACAATAACATCAAAGCAAGAT GCAATGGATTATATCACCTGGACTTACTTTTTCCGGCGTCTTATCATGAATCCCAGCTACTACAGCTTGG GTGACGTCAGCCAGGACTCCATAAACAAGTTCCTGTCACATCTGATTGGGCAGTCTCTGGTTGAATTGGA GCTTTCCCACTGCATTGAAGTTGGAGAGGATAATCGCACCATTGAACCACTGACATGTGGTCGAATTGCC TCCTATTACTATTTGAAGCACAAAACTGTCAAAATGTTCAAGGACCGTTTGAAACCTGAGTGCAGCACTG AGGAACTGCTGTCAATTCTGAGTGATGCAGAAGAATACACAGACTTACCAGTAAGACACAATGAGGACCA TACGAATAATGAATTGGCCAAATGTCTTCCCATTGAATTAAATCCTCATTCATTTGACAGCCCTCACACC AAAGCCCATCTCTTGCTTCAAGCGCATCTCAGCCGAGCCATGCTGCCCTGCCCAGATTATGATACTGATA CCAAAACAGTCTTAGACCAAGCTCTCAGAGTATGCCAGGCAATGCTGGATGTGGCTGCAAGCCAGGGATG GCTAGTGACCGTCCTGAATATCACCCACCTGATTCAGATGGTGATCCAAGGCCGATGGCTCAAGGACTCT TCTCTTCTCACAATACCAAACATAGAGCAGCACCACCTTCACCTTTTCAGGAAATGGAAGCCACCTGTAA AGAGCTCACATGCTAAGTGCCGAACATCCATTGAGTGCCTTCCTGAGCTGATCCATGCCTGTGAAGGGAA GGACCATGTGTTCAGCTCCATGGTAGAGAAAGAGTTACAGCCTGCAAAAACAAAGCAGGCATGGAATTTC TTATCTCGCTTGCCAGTGATAAATGTTGGCATAAGTGTTAAAGGCTCATGGGATGACTTAGTTGAAGGAC ATAATGAACTCTCCATCTCAACTCTGACAGCAGACAAACGGGATGAAAACAAATGGATCAAGTTGCATGC TGATCAGGAGTATGTGCTTCAAGTCAGCCTACAGAGAGTCCATTTTGGGCTTCCAAAGGGAAAACATGAG AATCACGCAGTTACTCCTCGATTTCCAAAATTAAAAGATGAAGGCTGGTTTTTGATATTAGGAGAAGTGG ATAAGAGAGAGCTGATGGCTGTAAAACGAGTAGGATTTGTTCGAACACATCATGATGCTTCCATCTCGTT CTTTACTCCTGAAACACCTGGAAGATATATCTTTACCCTGTATCTCATGAGTGACTGTTACCTCGGCCTA GACCAGCAGTATGACATTTATCTCAATGTCATAAAAGCAAACATTTCTACAAAGGACTCTGATGTCTTCA CTGACTTGTCAGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR219721 representing NM_198007 Red=Cloning site Green=Tags(s) MALPRLTGALRSFSNVTKQDNYNEEVADLKLKRSKLHEQVLDFGLTWKKIVKFLNEKLEKNKMQNINEDL KDILQAAKQIVGTDNGREAIESGAAFLFMTFHMTDSVGYMETKAIRQTFGPFPSSSATSACNATNRIISH FSQDDLTAFVQMAENPCNDRVVFGKNLAFSFDMYDLDHFDELPINGESQKTISLDYKKFLNEQFQEPYTP ELKPVEKTNGSLLWCEVEKYLNATLKEMTEAARVEDLCCTLYDMLASAKSGDELQDELFELLGPEGLDLI EKLLQNRITIVDRFLNSSSDHKFQVLQDSCKKILGENSKPNYGCQVTIQSEQEKQLMKQYRREEKRIARR EKKAGEDGEVSGEGVLPFDPKELRIQREHALLNARNAPILGRQRDVEFEKIRYPHVYDSQAQARETSAFI AGAKMILPEGIQRENTKLYEEVRIPYGEPMPVGFEEKPVYIKDLDEVGQLAFKGMKRLNRIQSIVFETAY NTNENMLICAPTGAGKTNIAMLTILHEIRQHFHQGVIKKNEFKIVYVAPMKALAAEMTNYFSKRLEPLGI VVKELTGDMQLSKSEILRTQMLVTTPEKWDVVTRKSVGDVALSQIVKLLILDEVHLLHEDRGPVLESIVA RTLRQVESTQSMIRILGLSATLPNYLDVATFLHVNPYIGLFYFDGRFRPVPLGQTFLGIKSTNKMQQLNN MDEVCYESVLKQVKAGHQVMVFVHARNATVRTAMSLIERAKNSGQISCFLPTEGPEYGHALKQVQKSRNK QVRELFSDGFSIHHAGMLRQDRNLVENLFSNGHIKVLVCTATLAWGVNLPAHAVVIKGTQIYAAKRGSFV DLGILDVMQIFGRAGRPQFDKFGEGIIITTHDKLSHYLSLLTQQNPIESQFLESLADNLNAEIALGTVTN VEEAVRWMSYTYLYVRMRANPLAYGISHKAYQIDPTLRKHREQLLIEVGQKLDKAKMIRFEERTGYFSST DLGRTASHFYIKYNTIETFNELFDAHKTEGDIFAIVSKAEEFDQIKVREEEIEELDALLNNFCELSAPGG VENSYGKINILLQTYISRGEMDSFSLISDSAYVAQNAARIVRALFEIALRKRWPTMTYRLLNLSKVIDKR LWGWASPLRQFSVLPPHILTRLEEKNLTVDKLKDMRKDEIGHILHHVNIGLKVKQCVHQIPSVTMEASIQ PITRTVLRVSLNIHPDFSWNDQVHGTVGEPWWIWVEDPTNDHIYHSEYFLALKKQVINKEAQLLVFTIPI FEPLPSQYYIRAVSDRWLGAEAVCIINFQHLILPERHPPHTELLDLQPLPITALGCKAYEALYNFSHFNP VQTQIFHTLYHTDCNVLLGAPTGSGKTVAAELAIFRVFNKYPTSKAVYIAPLKALVRERMDDWKIRIEEK LGKKVIELTGDVTPDMKSIAKADLIVTTPEKWDGVSRSWQNRSYVQQVNILIIDEIHLLGEERGPVLEVI VSRTNFISSHTEKPVRIVGLSTALANARDLADWLNIKQMGLFNFRPSVRPVPLEVHIQGFPGQHYCPRMA SMNKPAFQAIRSHSPAKPVLIFVSSRRQTRLTALELIAFLATEEDPKQWLNMDEQEMDNIIGTVRDSNLK LTLAFGIGMHHAGLHERDRKTVEELFVNCKVQVLIATSTLAWGVNFPAHLVIIKGTEYYDGKTRRYVDFP ITDVLQMMGRAGRPQFDDQGKAVILVHDIKKDFYKKFLYEPFPVESSLLGVLSDHLNAEIAGGTITSKQD AMDYITWTYFFRRLIMNPSYYSLGDVSQDSINKFLSHLIGQSLVELELSHCIEVGEDNRTIEPLTCGRIA SYYYLKHKTVKMFKDRLKPECSTEELLSILSDAEEYTDLPVRHNEDHTNNELAKCLPIELNPHSFDSPHT KAHLLLQAHLSRAMLPCPDYDTDTKTVLDQALRVCQAMLDVAASQGWLVTVLNITHLIQMVIQGRWLKDS SLLTIPNIEQHHLHLFRKWKPPVKSSHAKCRTSIECLPELIHACEGKDHVFSSMVEKELQPAKTKQAWNF LSRLPVINVGISVKGSWDDLVEGHNELSISTLTADKRDENKWIKLHADQEYVLQVSLQRVHFGLPKGKHE NHAVTPRFPKLKDEGWFLILGEVDKRELMAVKRVGFVRTHHDASISFFTPETPGRYIFTLYLMSDCYLGL DQQYDIYLNVIKANISTKDSDVFTDLSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_198007
ORF Size	6594 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_198007.2, NP_932124.2
RefSeq Size	7477 bp
RefSeq ORF	6597 bp
Locus ID	77987
UniProt ID	E9PZJ8
MW	250.6 kDa
Gene Summary	3'-5' DNA helicase involved in repair of alkylated DNA. Promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3, enabling ALKBH3 to process alkylated N3-methylcytosine (3mC) within double-stranded regions. Part of the ASC-1 complex that enhances NF-kappa-B, SRF and AP1 transactivation.[UniProtKB/Swiss-Prot Function]

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