Papd7 (NM_198600) Mouse Tagged ORF Clone
CAT#: MR219852
- TrueORF®
Papd7 (Myc-DDK-tagged) - Mouse PAP associated domain containing 7 (Papd7), transcript variant 1
ORF Plasmid: tGFP
"NM_198600" in other vectors (5)
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CNY 5,420.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | LAK-1; POLK; Pols; TRF4; TRF4-1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR219852 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCCTTGTCCTGAAGAAGCAGCCATGAGAAGGGAGGTGGTGAAACGGATCGAAACTGTGGTGAAAG ACCTCTGGCCCACAGCTGATGTGCAGATATTTGGCAGCTTTAGTACAGGCCTCTATCTTCCAACAAGTGA CATAGACCTGGTTGTCTTTGGAAAGTGGGAACGCCCTCCATTACAGTTGTTGGAACAAGCCCTCCGGAAG CACAACGTGGCTGAGCCGTGCTCCATCAAAGTTCTTGACAAAGCTACAGTGCCCATCATAAAGCTCACAG ATCAGGAGACTGAAGTTAAAGTCGACATCAGCTTTAACATGGAGACTGGCGTGCGGGCGGCAGAGTTCAT CAAGAATTACATGAAGAAGTACTCGCTACTGCCATACTTGATTTTAGTGCTGAAACAGTTCCTGCTGCAG AGGGACCTGAATGAGGTCTTCACAGGCGGGATCAGCTCCTACAGCCTCATCCTAATGGCCATCAGCTTTC TGCAGTTACATCCAAGAATCGATGCCCGGAGAGCTGATGAAAACCTGGGAATGCTTCTTGTAGAATTTTT TGAACTTTATGGAAGAAATTTTAATTACTTAAAAACTGGTATTAGAATAAAAGAAGGAGGTGCCTATATC GCCAAAGAAGAGATCATGAAAGCCATGACCAGTGGGTACCGACCATCGATGCTGTGCATTGAGGACCCCT TACTGCCTGGAAATGATGTTGGACGGAGTTCCTATGGGGCCATGCAGGTGAAGCAGGTGTTTGACTACGC TTACATTGTGCTCAGCCACGCTGTGTCACCGCTCGCCAGGTCTTACCCCAACAGGGATTCTGAAAGTACT TTAGGAAGAATCATCAAAGTCACCCAGGAAGTGATCGACTACCGGAGGTGGATCAAAGAAAAGTGGGGTA GCAGAATCCTCCCGTCTCCAGACCTTGACAACAGGATTAAGATAAAGGAGAGAATTACCACGTGCAATGG GGAGCAGATGCAGAGCCGGGAGCCCAGCTCCCCTTACACCCAGCGCCTGACTCTGTCCCTGTCCAGTCCC CAGCTGCTGTCTTCAGGCTCTTCTGCTTCTTCTGTGTCCTCACTTTCTGGAAGTGACATTGACTCCGACA CACCGCCCTGCACCACACCCAGTGTTTATCAGTTCAGCCTGCAAGCACCCACTACCCTGATGGCCAGCCT GCCCACAGCCTTGCCAATGCCCAGCAGCAAACCCCAGCCTGCTGCTTCCAGAACGTTGATCATGACAACA AACAACCAGACCAGGGTTACTATCCCTCCACCAACCCTCGGAGTCGCCCCTGTTCCTTGCAGACAGGCTG GTGTCGACGGAACCACATCTTTGAAGGCTGTTCACAGTGTAACTTCCCCAGCCATTCCCTCGGCATCCCC CAACCCACTGTCTAGTCCTCATCTGTATCACAAGCAGCACAATGGCATGAAACTGTCCATGAAAGGCTCC CACAACCACACTCAGGGTGGCGGCTACAGCTCTGTGGGCAGTGGAGCTGTGAGGCCTCCTGTAGGCAACC GAGGACATCATCAGTACAACCGCACCGGCTGGAGGAGAAAAAAGCACGCACACACAAGGGACAGCCTGCC CGTGAGTCTCAGCAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR219852 protein sequence
Red=Cloning site Green=Tags(s) MSPCPEEAAMRREVVKRIETVVKDLWPTADVQIFGSFSTGLYLPTSDIDLVVFGKWERPPLQLLEQALRK HNVAEPCSIKVLDKATVPIIKLTDQETEVKVDISFNMETGVRAAEFIKNYMKKYSLLPYLILVLKQFLLQ RDLNEVFTGGISSYSLILMAISFLQLHPRIDARRADENLGMLLVEFFELYGRNFNYLKTGIRIKEGGAYI AKEEIMKAMTSGYRPSMLCIEDPLLPGNDVGRSSYGAMQVKQVFDYAYIVLSHAVSPLARSYPNRDSEST LGRIIKVTQEVIDYRRWIKEKWGSRILPSPDLDNRIKIKERITTCNGEQMQSREPSSPYTQRLTLSLSSP QLLSSGSSASSVSSLSGSDIDSDTPPCTTPSVYQFSLQAPTTLMASLPTALPMPSSKPQPAASRTLIMTT NNQTRVTIPPPTLGVAPVPCRQAGVDGTTSLKAVHSVTSPAIPSASPNPLSSPHLYHKQHNGMKLSMKGS HNHTQGGGYSSVGSGAVRPPVGNRGHHQYNRTGWRRKKHAHTRDSLPVSLSR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_198600 |
ORF Size | 1626 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_198600.2, NP_941002.2 |
RefSeq Size | 4017 bp |
RefSeq ORF | 1629 bp |
Locus ID | 210106 |
UniProt ID | Q6PB75 |
MW | 60 kDa |
Gene Summary | Terminal nucleotidyltransferase that catalyzes preferentially the transfert of ATP and GTP on RNA 3' poly(A) tail creating a heterogeneous 3' poly(A) tail leading to mRNAs stabilization by protecting mRNAs from active deadenylation (By similarity). Also functions as a catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Has no terminal uridylyltransferase activity, and does not play a role in replication-dependent histone mRNA degradation via uridylation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC218555 | Papd7 (untagged) - Mouse PAP associated domain containing 7 (Papd7), transcript variant 1, (10ug) |
CNY 6,940.00 |
|
MG208654 | Papd7 (tGFP-tagged) - Mouse polymerase (DNA directed) sigma (Pols) |
CNY 5,230.00 |
|
MG219852 | Papd7 (tGFP-tagged) - Mouse PAP associated domain containing 7 (Papd7) transcript variant 1, (10ug) |
CNY 5,990.00 |
|
MR219852L3 | Lenti ORF clone of Papd7 (Myc-DDK-tagged) - Mouse PAP associated domain containing 7 (Papd7), transcript variant 1 |
CNY 7,320.00 |
|
MR219852L4 | Lenti ORF clone of Papd7 (mGFP-tagged) - Mouse PAP associated domain containing 7 (Papd7), transcript variant 1 |
CNY 7,320.00 |