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Krt28 (NM_027574) Mouse Tagged ORF Clone
CAT＃: MR219963
- TrueORF®
Krt28 (Myc-DDK-tagged) - Mouse keratin 28 (Krt28)
ORF Plasmid: tGFP
"NM_027574" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4733401L19Rik; Krt25d |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR219963 representing NM_027574
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCTCCGGTTTTCTGGCGGATCCAGGCACGTTGGCATACAGTCCGGATCCCTCAGGCCGCCCAGCG GAGGCGCAGGCTTTGCTGGGAGCAGCGTGGCTGGCGGTTCGGTTGCTGGTAGTGGATTTTCTTGGGCTTT GGGAGGGACACTGGGCAGTGCTCCTGGTGGGAGCCATGCCACTGGTGCCCTGGGAAATGTCTCTGGTGTT TGCTTCATCGGAAGTGAAGGGGGTCTCCTCTCTGGAAACGAGAAGGTCACCATGCAGAATCTTAACAACC GCTTGGCCTCTTACCTGGATAACGTAAAAGCTCTGGAGGAGGCCAACTCTGAGCTAGAGAGAAAGATCAA GACTTGGCATGAGAAATACGGACCGGGATCGTGCCGTGGACTTGACCGTGACTACAGCAAGTATCATCTA ACCATTGAGGACCTGAAAAGCAAGATCATCTCTTCCACGGCTGCTAATGCTAATATAATTCTGCAAATTG ATAACGCCAGACTGGCCGCAGACGATTTCCGACTAAAGTATGAAAACGAACTCACCCTTCACCAAAATGT CGAGGCAGACATCAATGGGTTGCGGCGCGTCCTTGACGAGCTAACCCTCTGCAGAACGGACCAAGAGCTG CAGTACGAATCCCTGAGTGAGGAGATGACTTATCTCAAGAAGAACCACGAAGAGGAAATGAAAGTCCTGC AGTGCGCAGCTGGAGGCAACGTGAACGTGGAGATGAACGCAGCCCCAGGCGTGGACCTCACCGTCCTTCT GAACAACATGCGAGCAGAGTACGAGGCCCTGGCTGAGCAGAACCGCAGGGACGCCGAGGCCTGGTTCCAA GAGAAGAGCGCCACGCTGCAGCAGCAGATCTCCAATGACTTGGGCGCCGCCACCTCAGCCAGGACCGAGC TGACAGAGCTGAAACGCTCCCTGCAGACCCTGGAGATCGAGCTGCAGTCCCTCTCAGCTACGAAACACTC TCTGGAGTGCTCCCTGGCGGAGACCGAGGGCAACTACTGCTCACAGCTGGCTCAGATCCAGGCTCAGATC AGCGCCCTGGAGGAGCAGCTGCACCAGGTGAGGACGGAGACCGAGGGCCAGAAGCTGGAACATGAGCAGC TGCTGGACATCAAGGCCCACCTGGAGAAGGAGATCGAGACCTACTGCCGCCTCATAGACGGAGACGAGAA TTCCTGCTCTGTCTCAAAGGGTTTTGAATCTGGAACCTCGGGGAATTCACCCAAAGATGTATCCAAAACC ACACTGGTGAAGACGGTGGTTGAAGAAATAGATCAGCGTGGGAAGGTGCTGTCCTCCAGGATCCACTCCA TCGAGGAAAAGATGTCTAAGATGAGCAACGGCAAGGCTGAGCAGAGGGTTCCCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR219963 representing NM_027574
Red=Cloning site Green=Tags(s) MSLRFSGGSRHVGIQSGSLRPPSGGAGFAGSSVAGGSVAGSGFSWALGGTLGSAPGGSHATGALGNVSGV CFIGSEGGLLSGNEKVTMQNLNNRLASYLDNVKALEEANSELERKIKTWHEKYGPGSCRGLDRDYSKYHL TIEDLKSKIISSTAANANIILQIDNARLAADDFRLKYENELTLHQNVEADINGLRRVLDELTLCRTDQEL QYESLSEEMTYLKKNHEEEMKVLQCAAGGNVNVEMNAAPGVDLTVLLNNMRAEYEALAEQNRRDAEAWFQ EKSATLQQQISNDLGAATSARTELTELKRSLQTLEIELQSLSATKHSLECSLAETEGNYCSQLAQIQAQI SALEEQLHQVRTETEGQKLEHEQLLDIKAHLEKEIETYCRLIDGDENSCSVSKGFESGTSGNSPKDVSKT TLVKTVVEEIDQRGKVLSSRIHSIEEKMSKMSNGKAEQRVPF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027574 |
| ORF Size | 1386 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027574.1, NP_081850.1 |
| RefSeq Size | 1489 bp |
| RefSeq ORF | 1389 bp |
| Locus ID | 70843 |
| UniProt ID | A6BLY7 |
| MW | 50.3 kDa |
| Gene Summary | Essential for the proper assembly of types I and II keratin protein complexes and the formation of keratin intermediate filaments in the inner root sheath (irs).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216328 | Krt28 (untagged) - Mouse keratin 28 (Krt28), (10ug) |
CNY 6,080.00 |
|
| MG219963 | Krt28 (tGFP-tagged) - Mouse keratin 28 (Krt28), (10ug) |
CNY 3,710.00 |
|
| MR219963L3 | Lenti ORF clone of Krt28 (Myc-DDK-tagged) - Mouse keratin 28 (Krt28) |
CNY 5,230.00 |
|
| MR219963L4 | Lenti ORF clone of Krt28 (mGFP-tagged) - Mouse keratin 28 (Krt28) |
CNY 5,230.00 |
