Gucy2f (NM_001007576) Mouse Tagged ORF Clone
CAT#: MR220267
- TrueORF®
Gucy2f (Myc-DDK-tagged) - Mouse guanylate cyclase 2f (Gucy2f)
ORF Plasmid: tGFP
"NM_001007576" in other vectors (4)
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CNY 7,712.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | A930002I06; GC-F; GC2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR220267 representing NM_001007576
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCTCGGACCCTGGCCTTTTTCTCGCCTTCTGTCCTGGTTTGCCATTTCCAGCAGACTGTCAGGAC AGCATGGCCTCCCTTCCTCCAAGTTCCTGAGATGTTTGTGCCTCTTGGCCCTTTTGCCTCTCCTTCGGTG GGGGCAAGCACTCCCCTACAAGATCGGGGTCATAGGTCCCTGGACATGTGATCCGTTCTTTTCCAAGGCC CTGCCTGAGGTTGCTGCTGCTTTAGCAATTGAGCGAATCAGCCGGGACAAGACATTTGACAGGAGTTACT CCTTCGAATACGTGATTCTTAACGAAGACTGTCAGACTTCAAAGGCCCTCGCCAGTTTCATTTCCCACCA GCAGATGGCCTCAGGATTTGTTGGGCCTGCCAACCCTGGCTTCTGCGAGGCAGCCTCACTCCTGGGAACC AGCTGGGACAAAGGGATTTTCTCTTGGGCTTGTGTGAATCATGAATTAGATAACAAACATAGCTTCCCAA CCTTCTCTCGGACACTCCCTTCTCCGATACGGGTGCTTGTAACCGTCATGAAGTATTTCCAGTGGGCACA TGCTGGGGTCATTTCCTCAGATGAAGACATTTGGATGCATACAGCCAATCGAGTCTCAAGTGCTCTTCGG AGCCAGGGCCTACCTGTAGGGGTCGTCCTGACCTCGGGACGAGACAGCCAGAGCATTCAGAAAGCTCTCC AGCAGATTCGCCAGGCAGACAGAATTCGCATAATCATAATGTGTATGCATTCAGCCTTGATTGGGGGAGA GACACAAACACACTTCTTGGAATTGGCTCATGATCTGAAAATGACAGATGGGACTTACGTCTTTGTCCCC TATGATGTACTGCTCTACAGTTTACCTTATAAGCACAGCCCCTACCAGGTCCTGAGGAACAATCCAAAGC TCCGGGAAGCCTATGATGCTGTGTTGACTATTACAGTGGAGTCCCATGAAAAGACCTTCTACGAAGCCTA TGCAGAAGCAGCAGCCCGCGGAGAAATTCCTGAGAAGCCTGACTCAAACCAAGTTTCACCGTTGTTTGGA ACCATCTACAATTCAATTTATTTTATCGCACAAGCCATGAATAATGCTATGAAAAAAAATGGACGGGCTA GTGCTGCCAGCCTGGTTCAGCATTCCAGAAACATGCAGTTCTATGGATTCAACCAGTTGATAAAGACAGA CTCAAATGGAAATGGGATTTCTGAATATGTAATCCTGGACACCAATGGGAAAGAATGGGAACTTCGTGGC ACCTACACTGTGGACATGGAAACTGAACTGCTAAGGTTCAGAGGGACCCCAATTCATTTCCCTGGCGGCA GACCTACTAGTGCAGATGCAAAATGCTGGTTTGCAGAAAGGAAGATCTGCCAAGGAGGTATTGACCCTGC CTTGGCCATGATGGTCTGCTTTGCTTTACTTATAGCCTTGCTGTCTATCAATGGATTTGCTTACTTTATA AGGCGACGTATAAATAAAATCCAGTTGATTAAAGGACCCAACAGAATCCTACTGACTTTGGAAGATGTGA CATTTATTAATCCCCACTTTGGCAGTAAGAGAGGAAGCCGTGCCAGTGTAAGCTTCCAGATCATCTCAGA AGTTCAAAGTGGGAGGTCCCCAAGGCTGTCCTTTTCTTCAGGTAGTCTAACTCCAGCTACATATGAGAAC TCCAACATAGCAATCTATGAGGGTGACTGGGTATGGCTGAAAAAGTTCCCTCCTGGAGATTTTGGAGACA TTAAGTCTATCAAATCGAGTGCAAGTGATGTGTTTGAAATGATGAAGGACCTGCGGCATGAAAATGTCAA CCCTCTATTGGGTTTCTTCTATGATTCGGGGATGTTTGCCATCGTGTCAGAATTTTGTTCTCGAAGGAGC CTAGAAGACATACTTACAAATGATGATGTGAAACTTGATTGGATGTTCAAATCATCACTACTGCTTGACC TCATCAAGGGGATGAAATACTTACACCACAGAGAATTTATTCACGGGAGGCTAAAGTCTCGGAACTGTGT GGTAGATGGGCGTTTTGTCCTAAAAGTGACAGATTATGGCTTTAATGACATTTTAGAAATGCTGAGACTC TCTGAAGAGGAACCTTCTGAAGAAGAGCTGCTGTGGACGGCCCCTGAACTGTTAAGAGCCCCTGGGGGCA TCAGGTTAGGTTCATTTGCAGGAGACGTCTATAGCTTTGCCATCATCATGCAAGAAGTGATGGTTCGGGG TGCTCCTTTCTGCATGATGGATCTGCCTGCCAAAGAAATCATAGACAGACTTAAAATGCCTCCCCCTGTG TACAGACCCGTGGTTTCTCCTGAGTATGCCCCTGCAGAATGTCTCCAGCTGATGAAGCAGTGCTGGGCTG AGGCGTCAGAACAGCGACCAACTTTTGATGAAATATTTAACCAGTTTAAAACTTTCAACAAAGGGAAAAA GACTAATATTATTGATTCTATGCTTCGGATGTTGGAGCAGTATTCTAGCAACTTGGAAGATTTGATCCGG GAACGGACTGAAGAATTGGAAATTGAAAAACAGAAAACGGAAAAGCTTCTAACACAGATGCTACCACTAT CAGTTGCTGAATCCCTCAAGAAGGGCTGTACAGTTGAACCTGAGGGATTTGACTTGGTCACCTTGTACTT CAGTGACATTGTGGGCTTCACCACCATCTCAGCCATGAGTGAGCCCATTGAAGTTGTGGACCTTCTGAAT GACCTGTACACACTCTTTGATGCCATCATTGGCAGTCATGATGTCTATAAAGTAGAGACCATTGGAGATG CCTACATGGTGGCCTCAGGCCTTCCCAAGAGGAATGGCAGTAGGCATGCAGCTGAGATTGCAAACATGTC CCTGGATATCCTGAGTTCTGTGGGTACCTTTAAGATGCGACACATGCCAGAGGTGCCAGTCCGAATTCGA ATAGGCCTGCACTCGGGGCCTGTTGTTGCTGGAGTGGTGGGCCTCACCATGCCCAGATATTGCTTGTTTG GGGACACTGTGAACACAGCTTCTCGAATGGAATCAACAGGGCTACCATACCGAATTCATGTCAGTCTCAG CACAGTAACAATTCTTCAAACTCTGAGTGAAGGCTATGAAGTAGAGCTTCGTGGAAGAACAGAGCTCAAG GGCAAAGGCACAGAGGAGACCTTTTGGCTTGTTGGAAAAAAGGGTTTCACCAAACCTCTTCCTGTACCCC CACCAGTGGGCAAAGATGGGCAAGTGGGCCATGGCTTGCAACCAGCAGAGATTGCAGCATTCCAAAGGAG AAAAGCAGAGAGGCAGTTGGTGCGAAACAAGCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220267 representing NM_001007576
Red=Cloning site Green=Tags(s) MFLGPWPFSRLLSWFAISSRLSGQHGLPSSKFLRCLCLLALLPLLRWGQALPYKIGVIGPWTCDPFFSKA LPEVAAALAIERISRDKTFDRSYSFEYVILNEDCQTSKALASFISHQQMASGFVGPANPGFCEAASLLGT SWDKGIFSWACVNHELDNKHSFPTFSRTLPSPIRVLVTVMKYFQWAHAGVISSDEDIWMHTANRVSSALR SQGLPVGVVLTSGRDSQSIQKALQQIRQADRIRIIIMCMHSALIGGETQTHFLELAHDLKMTDGTYVFVP YDVLLYSLPYKHSPYQVLRNNPKLREAYDAVLTITVESHEKTFYEAYAEAAARGEIPEKPDSNQVSPLFG TIYNSIYFIAQAMNNAMKKNGRASAASLVQHSRNMQFYGFNQLIKTDSNGNGISEYVILDTNGKEWELRG TYTVDMETELLRFRGTPIHFPGGRPTSADAKCWFAERKICQGGIDPALAMMVCFALLIALLSINGFAYFI RRRINKIQLIKGPNRILLTLEDVTFINPHFGSKRGSRASVSFQIISEVQSGRSPRLSFSSGSLTPATYEN SNIAIYEGDWVWLKKFPPGDFGDIKSIKSSASDVFEMMKDLRHENVNPLLGFFYDSGMFAIVSEFCSRRS LEDILTNDDVKLDWMFKSSLLLDLIKGMKYLHHREFIHGRLKSRNCVVDGRFVLKVTDYGFNDILEMLRL SEEEPSEEELLWTAPELLRAPGGIRLGSFAGDVYSFAIIMQEVMVRGAPFCMMDLPAKEIIDRLKMPPPV YRPVVSPEYAPAECLQLMKQCWAEASEQRPTFDEIFNQFKTFNKGKKTNIIDSMLRMLEQYSSNLEDLIR ERTEELEIEKQKTEKLLTQMLPLSVAESLKKGCTVEPEGFDLVTLYFSDIVGFTTISAMSEPIEVVDLLN DLYTLFDAIIGSHDVYKVETIGDAYMVASGLPKRNGSRHAAEIANMSLDILSSVGTFKMRHMPEVPVRIR IGLHSGPVVAGVVGLTMPRYCLFGDTVNTASRMESTGLPYRIHVSLSTVTILQTLSEGYEVELRGRTELK GKGTEETFWLVGKKGFTKPLPVPPPVGKDGQVGHGLQPAEIAAFQRRKAERQLVRNKP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001007576 |
ORF Size | 3324 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001007576.2, NP_001007577.1 |
RefSeq Size | 8122 bp |
RefSeq ORF | 3327 bp |
Locus ID | 245650 |
UniProt ID | Q5SDA5 |
MW | 124.9 kDa |
Gene Summary | Probably plays a specific functional role in the rods and/or cones of photoreceptors. It may be the enzyme involved in the resynthesis of cGMP required for recovery of the dark state after phototransduction (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223603 | Gucy2f (untagged) - Mouse guanylate cyclase 2f (Gucy2f), (10ug) |
CNY 14,160.00 |
|
MG220267 | Gucy2f (tGFP-tagged) - Mouse guanylate cyclase 2f (Gucy2f), (10ug) |
CNY 9,220.00 |
|
MR220267L3 | Lenti ORF clone of Gucy2f (Myc-DDK-tagged) - Mouse guanylate cyclase 2f (Gucy2f) |
CNY 10,260.00 |
|
MR220267L4 | Lenti ORF clone of Gucy2f (mGFP-tagged) - Mouse guanylate cyclase 2f (Gucy2f) |
CNY 10,260.00 |