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Gpr75 (NM_175490) Mouse Tagged ORF Clone
CAT＃: MR220478
- TrueORF®
Gpr75 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 75 (Gpr75)
ORF Plasmid: tGFP
"NM_175490" in other vectors (4)
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CNY 4,032.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR220478 representing NM_175490
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACACAAGTGCCCCGCTTCAGAATGTCCCCAATGCCACCTTGCTAAACATGCCTCCCCTGCACGGGG GAAATAGCACTTCTCTCCAGGAAGGTCTTCGAGATTTTATCCACACAGCCACCTTGGTGACCTGCACTTT TCTGCTTGCCATCATCTTCTGTCTAGGCTCTTATGGAAATTTTATTGTCTTCTTGTCTTTCTTTGACCCA TCCTTCAGGAAGTTCAGAACCAACTTTGATTTCATGATCTTGAACCTGTCTTTCTGTGATCTGTTCATCT GTGGGGTCACAGCCCCCATGTTCACCTTCGTGCTGTTCTTCAGCTCAGCCAGTAGCATCCCAGATAGCTT CTGCTTCACCTTCCACCTTACCAGCTCAGGCTTCGTCATCATGTCCCTCAAGATGGTGGCTGTGATTGCT CTGCACCGGCTCCGGATGGTGATGGGGAAGCAGCCTAATTGTACAGCCTCCTTTTCCTGCATCTTGCTCC TTACCCTTCTTCTCTGGGCGACCAGCTTTACACTTGCCACCTTGGCTACACTGAGAACCAATAAGTCCCA CCTGTGTCTCCCCATGTCCAGTCTTATGGATGGGGAAGGGAAAGCCATTCTGTCTCTGTATGTTGTTGAC TTTACCTTCTGTGTTGCTGTGGTGTCTGTCTCTTATATTATGATTGCTCAAACCCTTCGGAAAAATGCTC AAGTAAAAAAGTGCCCCCCGGTGATCACAGTTGATGCTTCCAGACCACAGCCATTCATGGGGGCCTCTGT GAAGGGAAATGGAGATCCCATCCAGTGCACCATGCCAGCTCTGTATAGGAACCAGAATTATAACAAACTG CAGCACAGTCAAACTCATGGATACACTAAGAATATCAACCAGATGCCAATCCCCTCAGCCAGTCGACTCC AGCTGGTATCAGCCATCAACTTCTCTACTGCCAAGGATTCCAAAGCCGTGGTCACCTGTGTGGTCATCGT GTTGTCAGTCCTGGTGTGCTGTCTTCCTCTTGGGATTTCCCTGGTGCAAATGGTTCTGTCTGACAATGGC AGTTTTATCCTTTACCAGTTTGAACTGTTTGGATTTACTCTGATATTTTTCAAGTCAGGATTAAATCCTT TTATATATTCTCGGAACAGTGCTGGGCTGAGAAGGAAAGTACTCTGGTGCCTGAGATACACTGGCCTGGG CTTTCTCTGCTGCAAACAGAAAACTCGACTTCGGGCCATGGGAAAAGGGAACCTTGAAATCAATAGAAAC AAATCTTCTCATCATGAGACAAACTCTGCCTACATGCTGTCTCCAAAACCACAGAGAAAATTTGTGGACC AGGCTTGTGGCCCAAGTCACTCAAAGGAAAGTGCAGCGAGTCCCAAAGTTTCTGCTGGACATCAACCCTG TGGTCAAAGCAGTTCCACACCCATCAACACTAGGATTGAACCTTACTATAGCATCTATAACAGCAGCCCT TCCCAGCAGGAGAGCGG[AT]CCGGCAAACTTGCCTCCAGTGAACTCTTTTGGGTTTGCCAGTTCCTACA TCGCCATGCACTATTACACCACCAATGATTTGATGCAGGAATATGACAGCACGTCAGCAAAACAAATTCC TATCCCCTCTGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220478 representing NM_175490
Red=Cloning site Green=Tags(s) MNTSAPLQNVPNATLLNMPPLHGGNSTSLQEGLRDFIHTATLVTCTFLLAIIFCLGSYGNFIVFLSFFDP SFRKFRTNFDFMILNLSFCDLFICGVTAPMFTFVLFFSSASSIPDSFCFTFHLTSSGFVIMSLKMVAVIA LHRLRMVMGKQPNCTASFSCILLLTLLLWATSFTLATLATLRTNKSHLCLPMSSLMDGEGKAILSLYVVD FTFCVAVVSVSYIMIAQTLRKNAQVKKCPPVITVDASRPQPFMGASVKGNGDPIQCTMPALYRNQNYNKL QHSQTHGYTKNINQMPIPSASRLQLVSAINFSTAKDSKAVVTCVVIVLSVLVCCLPLGISLVQMVLSDNG SFILYQFELFGFTLIFFKSGLNPFIYSRNSAGLRRKVLWCLRYTGLGFLCCKQKTRLRAMGKGNLEINRN KSSHHETNSAYMLSPKPQRKFVDQACGPSHSKESAASPKVSAGHQPCGQSSSTPINTRIEPYYSIYNSSP SQQESXXPANLPPVNSFGFASSYIAMHYYTTNDLMQEYDSTSAKQIPIPSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175490 |
| ORF Size | 1623 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175490.3, NM_175490.4, NP_780699.2 |
| RefSeq Size | 1623 bp |
| RefSeq ORF | 1623 bp |
| Locus ID | 237716 |
| UniProt ID | Q6X632 |
| MW | 60 kDa |
| Gene Summary | G protein-coupled receptor that is activated by the chemokine CCL5/RANTES. Probably coupled to heterotrimeric Gq proteins, it stimulates inositol trisphosphate production and calcium mobilization upon activation. Together with CCL5/RANTES, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. CCL5/RANTES may also regulate insulin secretion by pancreatic islet cells through activation of this receptor.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218627 | Gpr75 (untagged) - Mouse G protein-coupled receptor 75 (Gpr75), (10ug) |
CNY 4,432.00 |
|
| MG220478 | Gpr75 (tGFP-tagged) - Mouse G protein-coupled receptor 75 (Gpr75), (10ug) |
CNY 5,040.00 |
|
| MR220478L3 | Lenti ORF clone of Gpr75 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 75 (Gpr75) |
CNY 6,432.00 |
|
| MR220478L4 | Lenti ORF clone of Gpr75 (mGFP-tagged) - Mouse G protein-coupled receptor 75 (Gpr75) |
CNY 6,460.00 |
