Ehd2 (NM_153068) Mouse Tagged ORF Clone
CAT#: MR220542
- TrueORF®
Ehd2 (Myc-DDK-tagged) - Mouse EH-domain containing 2 (Ehd2)
ORF Plasmid: tGFP
"NM_153068" in other vectors (4)
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CNY 4,048.00
CNY 4,560.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | BC027084; C130052H20Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR220542 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCAGCTGGCTGAAGAAGGGCGGGGCCCGGGGCCAGCGACCTGAGGCCATCCGCACCGTGACCTCAT CCCTCAAGGAGCTGTATCGCACGAAACTTCTTCCCCTGGAGGAACACTACCGTTTTGGGTCTTTCCATTC ACCGGCCCTTGAAGATGCTGACTTCGACGGCAAACCCATGGTGCTGGTGGCCGGCCAGTATAGCACCGGC AAAACCAGCTTCATCCAGTACCTTCTGGAGCAGGAAGTCCCTGGCTCCCGCGTGGGTCCCGAGCCCACCA CCGACTGCTTCGTGGCTGTCATGCATGGCGAGACAGAGGGCACCGTGCCCGGAAATGCTCTCGTTGTGGA CCCAGAGAAGCCCTTCCGCAAACTCAACCCTTTTGGAAACACTTTTCTCAACAGGTTTATGTGCGCCCAA CTCCCCAACCAGGTCCTGGAGAGCATCAGCATCATCGACACGCCTGGCATCCTATCAGGTGCAAAACAGA GAGTGAGCCGTGGCTACGACTTCCCAGCTGTCCTGCGCTGGTTTGCTGAACGCGTGGACCTCATCATCTT GCTCTTTGATGCCCACAAGCTGGAGATTTCCGATGAATTCTCGGAGGCCATCGGTGCCTTGCGTGGCCAC GAGGATAAGATCCGCGTGGTACTCAACAAGGCAGACATGGTGGAGACACAGCAGCTGATGCGCGTCTACG GGGCGCTCATGTGGGCGCTGGGCAAGGTGGTGGGCACGCCTGAGGTCCTTCGCGTCTACATTGGCTCCTT CTGGTCCCAGCCGCTCCTTGTGCCGGACAACCGGCGTCTTTTCGAGCTGGAGGAACAGGACCTCTTCCGT GATATCCAAGGCCTACCGCGCCATGCGGCGCTGCGCAAGCTCAACGACCTAGTGAAACGGGCGCGGCTGG TGCGGGTACACGCTTACATCATCAGCTACCTGAAGAAAGAGATGCCCACGGTGTTCGGGAAGGAAAACAA GAAGAAGCAGCTGATCCTGAAGCTGCCTGTCATCTTTGCGAAGATCCAGCTGGAGCACCACATCTCCCCT GGAGACTTTCCTGACTGCCAGAAGATGCAGGAGCTGTTGATGGCACATGACTTCACCAAGTTCCACTCGC TAAAGCCGAAGCTGCTGGAGGCGCTAGATGACATGCTGGCCCAAGACATTGCCAAGCTTATGCCGCTGCT TCGGCAGGAGGAGCTGGAGAGTGTCGAGGCTGGAGTGCAGGGCGGTGCGTTTGAGGGCACCCGCATGGGC CCCTTCGTGGAGCGGGGCCCTGATGAAGCAATAGAGGATGGTGAGGAGGGCTCAGAAGATGATGCCGAAT GGGTGGTGACCAAGGACAAGTCCAAATACGATGAAATCTTCTACAACTTAGCTCCTGCTGATGGCAAGCT GAGTGGCTCTAAGGCCAAAACCTGGATGGTGGGCACAAAGCTACCCAACTCTGTGCTGGGGAGAATCTGG AAGCTGAGCGATGTCGACCGAGACGGCATGCTGGATGATGAGGAGTTTGCCTTGGCCAGCCACCTCATTG AGGCTAAGCTCGAAGGCCATGGTCTACCCACCAACCTACCCCGCCGCCTGGTGCCGCCTTCTAAGCGACG ACAGAAGGGCTCTGCTGAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR220542 protein sequence
Red=Cloning site Green=Tags(s) MFSWLKKGGARGQRPEAIRTVTSSLKELYRTKLLPLEEHYRFGSFHSPALEDADFDGKPMVLVAGQYSTG KTSFIQYLLEQEVPGSRVGPEPTTDCFVAVMHGETEGTVPGNALVVDPEKPFRKLNPFGNTFLNRFMCAQ LPNQVLESISIIDTPGILSGAKQRVSRGYDFPAVLRWFAERVDLIILLFDAHKLEISDEFSEAIGALRGH EDKIRVVLNKADMVETQQLMRVYGALMWALGKVVGTPEVLRVYIGSFWSQPLLVPDNRRLFELEEQDLFR DIQGLPRHAALRKLNDLVKRARLVRVHAYIISYLKKEMPTVFGKENKKKQLILKLPVIFAKIQLEHHISP GDFPDCQKMQELLMAHDFTKFHSLKPKLLEALDDMLAQDIAKLMPLLRQEELESVEAGVQGGAFEGTRMG PFVERGPDEAIEDGEEGSEDDAEWVVTKDKSKYDEIFYNLAPADGKLSGSKAKTWMVGTKLPNSVLGRIW KLSDVDRDGMLDDEEFALASHLIEAKLEGHGLPTNLPRRLVPPSKRRQKGSAE SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_153068 |
ORF Size | 1632 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_153068.3, NP_694708.2 |
RefSeq Size | 3058 bp |
RefSeq ORF | 1632 bp |
Locus ID | 259300 |
UniProt ID | Q8BH64 |
MW | 61.2 kDa |
Gene Summary | ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis (PubMed:24508342). Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4 (PubMed:14676205). Required for fusion of myoblasts to skeletal muscle myotubes. Required for normal translocation of FER1L5 to the plasma membrane (PubMed:18502764, PubMed:21177873). Regulates the equilibrium between cell surface-associated and cell surface-dissociated caveolae by constraining caveolae at the cell membrane (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
EHD2 modulates Dll4 endocytosis during blood vessel development, et al. EHD2 modulates Dll4 endocytosis during blood vessel development
,null,
Microcirculation (New York, N.y. : 1994)
,PubMed ID 34820962
[Ehd2]
|
Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC218658 | Ehd2 (untagged) - Mouse EH-domain containing 2 (Ehd2), (10ug) |
CNY 6,940.00 |
|
MG220542 | Ehd2 (tGFP-tagged) - Mouse EH-domain containing 2 (Ehd2), (10ug) |
CNY 5,040.00 |
|
MR220542L3 | Lenti ORF clone of Ehd2 (Myc-DDK-tagged) - Mouse EH-domain containing 2 (Ehd2) |
CNY 6,460.00 |
|
MR220542L4 | Lenti ORF clone of Ehd2 (mGFP-tagged) - Mouse EH-domain containing 2 (Ehd2) |
CNY 6,460.00 |