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Igsf5 (NM_001177886) Mouse Tagged ORF Clone
CAT＃: MR220800
- TrueORF®
Igsf5 (Myc-DDK-tagged) - Mouse immunoglobulin superfamily, member 5 (Igsf5), transcript variant 1
ORF Plasmid: tGFP
"NM_001177886" in other vectors (4)
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CNY 3,710.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2010003D20Rik; Jam4; JCAM |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR220800 representing NM_001177886
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGCAGCTGGAGAGATGTCCTGGCTGTGCTGGTCATCCTGGCTCAGCTGACAGCTTCCGGATCCA GTTATCAGATCATAGAAGGTCCTCAGAATGTAACAGTCCTAAAGGACTCAGAGGCTCACTTCAACTGCAC CGTGACTCACGGCTGGAAGCTTCTCATGTGGACTCTTAACCAAATGGTGGTGCTGAGTCTCACCACCCAA GGACCCATCATCACCAACAACCGCTTCACCTATGCCAGTTACAACAGCACTGACAGCTTCATCTCGGAGT TGATCATCCATGATGTGCAGCCCAGTGACTCGGGATCCGTGCAATGCAGCCTGCAGAACAGCCATGGGTT TGGATCTGCCTTCCTCTCAGTGCAAGTCATGGGGACCCTGAACATTCCTAGCAACAACCTTATAGTCACT GAGGGTGAACCCTGTAATGTGACTTGCTATGCCGTGGGCTGGACCTCACTCCCGGATATTTCCTGGGAGC TTGAGGTTCCCGTAAGCCATTCGAGTTACAATTCCTTTCTGGAGCCGGGCAACTTTATGAGGGTCTTGAG TGTCCTGGACCTCACACCACTGGGCAACGGGACCTTGACTTGTGTGGCAGAGCTGAAGGACTTGCAGGCC AGCAAGTCCTTAACTGTCAACCTGACTGTGGTTCAGCCTCCACCTGACAGTATTGGAGAGGAAGGCCCAG CACTGCCGACCTGGGCCATCATCCTGCTGGCAGTGGCCTTTTCCTTGCTCTTGATCCTGATCATTGTTTT GATTATAATATTCTGTTGCTGTTGTGCCTCCAGGAGAGAAAAGGAAGAATCTACTTATCAAAATGAAATA AGGAAATCTGCAAACATGAGGACAAACAAAGCAGATCCGGAGACAAAGTTAAAAAGTGGAAAGGAAAACT ACGGGTACAGTTCGGATGAGGCAAAGGCTGCACAGACTGCATCTCTCCCTCCTAAATCTGCTGAAGTCAG CCTTCCAGAAAAACGCAGCAGTAGCCTTCCTTATCAGAGGTCGACTCATGGAATGGTGGATGCTGAGAGG TCCAAGGTTCACAACAATCTGAAAAACCAGGAGGGAGTTGATGACATGGCTGCAGCCTGTGGTGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220800 representing NM_001177886
Red=Cloning site Green=Tags(s) MEGSWRDVLAVLVILAQLTASGSSYQIIEGPQNVTVLKDSEAHFNCTVTHGWKLLMWTLNQMVVLSLTTQ GPIITNNRFTYASYNSTDSFISELIIHDVQPSDSGSVQCSLQNSHGFGSAFLSVQVMGTLNIPSNNLIVT EGEPCNVTCYAVGWTSLPDISWELEVPVSHSSYNSFLEPGNFMRVLSVLDLTPLGNGTLTCVAELKDLQA SKSLTVNLTVVQPPPDSIGEEGPALPTWAIILLAVAFSLLLILIIVLIIIFCCCCASRREKEESTYQNEI RKSANMRTNKADPETKLKSGKENYGYSSDEAKAAQTASLPPKSAEVSLPEKRSSSLPYQRSTHGMVDAER SKVHNNLKNQEGVDDMAAACGA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001177886 |
| ORF Size | 1116 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001177886.1, NP_001171357.1 |
| RefSeq Size | 1508 bp |
| RefSeq ORF | 1119 bp |
| Locus ID | 72058 |
| MW | 40.9 kDa |
| Gene Summary | Provides, together with MAGI1, an adhesion machinery at tight junctions, which may regulate the permeability of kidney glomerulus and small intestinal epithelial cells. Mediates calcium-independent homophilic cell adhesion. In testis, it may function as a cell adhesion molecule rather than a tight-junction protein. It may participate in the adhesion between spermatogonia-spermatogonia, spermatogonia-Sertoli cells, and Sertoli cells-Sertoli cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC211282 | Igsf5 (untagged) - Mouse immunoglobulin superfamily, member 5 (Igsf5), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
| MG220800 | Igsf5 (tGFP-tagged) - Mouse immunoglobulin superfamily member 5 (Igsf5) transcript variant 1, (10ug) |
CNY 7,456.00 |
|
| MR220800L3 | Lenti ORF clone of Igsf5 (Myc-DDK-tagged) - Mouse immunoglobulin superfamily, member 5 (Igsf5), transcript variant 1 |
CNY 5,610.00 |
|
| MR220800L4 | Lenti ORF clone of Igsf5 (mGFP-tagged) - Mouse immunoglobulin superfamily, member 5 (Igsf5), transcript variant 1 |
CNY 5,610.00 |
