Camk2b (NM_001174054) Mouse Tagged Lenti ORF Clone
CAT#: MR221054L3
- LentiORF®
Lenti ORF clone of Camk2b (Myc-DDK-tagged) - Mouse calcium/calmodulin-dependent protein kinase II, beta (Camk2b), transcript variant 3
"NM_001174054" in other vectors (4)
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CNY 7,220.00
CNY 600.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CaMKII |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR221054).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001174054 |
ORF Size | 1587 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001174054.1, NP_001167525.1 |
RefSeq Size | 3986 bp |
RefSeq ORF | 1590 bp |
Locus ID | 12323 |
Gene Summary | Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Also regulates the migration of developing neurons (PubMed:29100089). Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2 (PubMed:21752990).[UniProtKB/Swiss-Prot Function] |
Documents
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FAQs |
SDS |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC217557 | Camk2b (untagged) - Mouse calcium/calmodulin-dependent protein kinase II, beta (Camk2b), transcript variant 3, (10ug) |
CNY 6,840.00 |
|
MG221054 | Camk2b (tGFP-tagged) - Mouse calcium/calmodulin-dependent protein kinase II beta (Camk2b) transcript variant 3, (10ug) |
CNY 5,890.00 |
|
MR221054 | Camk2b (Myc-DDK-tagged) - Mouse calcium/calmodulin-dependent protein kinase II, beta (Camk2b), transcript variant 3 |
CNY 5,320.00 |
|
MR221054L4 | Lenti ORF clone of Camk2b (mGFP-tagged) - Mouse calcium/calmodulin-dependent protein kinase II, beta (Camk2b), transcript variant 3 |
CNY 7,220.00 |