Espl1 (NM_001014976) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AL024103; AU045071; Cerp; ESP1; PRCE; SSE
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR221057 representing NM_001014976 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAACTTCAAAGGAGTCAACTTCGCCACTCTGCTCTGCAGCAAGGAGGAGACCCAGCAGCTGCTGC CTGACTTGAAGGAGTTCCTGTCCAGATCCCGGACTGATTTCCCCAGCAGCCGAACAGATGCCGAAAGAAG ACAAATTTGCGATACCATCCTGAGGGCTTGCACCCAGCAGTTGACTGCCAAGCTCGATTGCCCTGGGCAC CTGAGGAGTATTTTGGACCTGGCAGAGCTGGCCTGTGATGGCTATTTGTTGTCTACTCCACAGCGCCCTC CCCTCTACCTGGAACGCATTCTCTTCATCCTACTTCGCAATGGTTCCACTCAGGGAAGCCCAGACACTGT GCTGCGCCTTGCCCAGCCTCTCCATGCCTGCTTGGTTCAGAACTCTGGAGAGGCTGCCCCTCAGGACTAT GAGGCCGTGACCCGGGGCAGCTTCTCTCTGTTTTGGAAGGGAGCAGAGGCACTGTTGGAGCGGCGAGCTG CGTTCTCAACTCGGCTGAATGCCTTGAGCTTCTTGGTGCTCTTGGAGGATGGCAGTGTCCCCTGTGAGGT CCCTCACTTTGCTTCTCCAACCGCCTGTCGTTTAGTTGCCGCCTATCAGCTGTACGATGCTACAGGACAA GGTCTAGATGAAGCTGATGCCGACTTCCTGTATGAAGTGCTTTCTAGGCACCTGATCAGAGTCTTAGTAG GTGAGGGTGGTAGCTCTCCCGGTCCTCTCTCGCCTCAGAGGGCCCTCTGCCTCTTGGAAATCACCCTGGA ACACTGCCGTCGGCTCTGCTGGAACCACCACCACCGCCAGGCTGCCAGAGCAGTGGAGAGGGCTCGTAAT CACCTAGAGAAGACCAGTGTAGCTCCTAGCCTCCAGTTATGCCAGATGGGGGTTGAGCTGTTGGAAGCTG TAGAAGAAAGACCAGGGGCAGTGGCCCAGCTTCTGAGGAAGGCAGCAGCCGTTCTTATCAACAGTATCGA GGCGCCGTCGCCCCCGCTCCGGGCATTGTACGACAGCTGCCAGTTCTTCCTTTCAGGCCTGGAGAGAGGC ATCAGGAGGCACTGTGGACTTGATGCCATATTGAGTCTCTTTGCTTTTCTTGGGGGATACAGCTCCCTTG TCCGGCACCTGAGAGAAGTCTCTGAAGCATCTTCCAAGCAGCAGCAGTGCTTGCTTCAGATGCACTTCCA GGGTTTCCACCTCTTCACCGGGATTGTGTATGACTTTGCTCAAGGCTGTCAGGCAACTGAGCTTGCTCAG CTAGTGGACGGTTGCAGATCTGCTGCTGTCTGGATGTTGGAGGCCTTAGAGGGCCTGTCGGGTGGAGAGC TGGCTGACTACCTGAGCATGACTGCCTCTTACACCAGCAACTTGGCCTACAGCTTCTTTAGTCAGAAGCT CTATGAGGAGGCCTGCGTCATCTCAGAGCCAGTCTGTCAGCACTTGGGCTCGGCAACGTCGGGTGCTTGT CCTGAAGTGCCTCCTGAGAAGCTACATAGGTGCTTCAGGCTGCATGTGGAGAGTTTGAAGAAACTGGGTA AACAGGCCCAGGGCTGCAAGATGGTGACCTTATGGCTGGCAGCTCTGAAACCCTACAGTCTGGAACACAT GGTCGAGCCAGTCACTTTCTGGGTTCGAGTCAAAATGGATGCATCCAGGGCAGGAGACAAGGAGCTACAA CTTCAGACTTTGCGAGACAGCCTAAGCTGCTGGGACCCAGAGACACAGTCTCTGCTTCTCAGGGAGGAGC TGCGGGCCTACAAGTCAGTACGGGCTGACACTGGGCAGGAGCGCTTCAACATCATCTGTGATCTGCTCGA GCTGAGCCCTGAAGAGACAGCAGCTGGAGCCTGGGCACGAGCTACCTACCTGGTGGAACTGGCCCAAGTG CTGTGCTACCACAACTTTACCCAGCAGACCAACTGCTCTGCCTTGGATGCTGTCCAGGAAGCCCTGCAGC TTCTGGAGTCCGTGAGTCCTGAGGCCCAGGAGCAGGATCGACTTCTGGATGATAAAGCACAGGCCTTGTT ATGGCTTTACATCTGTACCTTAGAGGCCAAGATGCAGGAAGGTATTGAGCGGGATCGGAGAGCCCAGGCC CCTAGTAACTTGGAGGAATTTGAAGTCAATGACCTGAACTATGAAGATAAACTGCAGGAAGATCGTTTTC TGTATAGTAGCATTGCCTTCAATCTGGCTGCTGATGCAGCTCAGTCCAAATGCCTGGACCAGGCCCTGAC TCTGTGGAAAGAAGTGCTCACCAAGGGGCGAGCCCCAGCTGTGCGGTGTCTCCAGCAGACTGCAGCCTCC CTGCAAATCCTAGCGGCCGTCTATCAGTTGGTGGCAAAGCCCTTGCAGGCTCTGGAGACCCTCCTGCTCC TGCAGATTGTATCTAAGAGACTGCAGGACCACGCAAAGGCAGCCAGCTCTTCCTGCCAGCTTACCCAGCT ACTCTTGAACCTTGGCTGTCCCAGCTATGCGCAGTTGTATCTGGAAGAGGCGGAGTCAAGCCTGAGGAGC CTGGACCAGACAAGTGACGCGTGCCAGCTGCTGTCCCTGACCTGTGCTCTGCTTGGAAGTCAGCTCTGCT GGGCTTGCCAGAAGGTAACTGCAGGCGTTTCTTTGTTGCTTTCTGTGCTTCGGGATCCTGCCCTCCAGAA GTCATCTAAGGCTTGGTACTTGCTACGTGTCCAGGCCCTGCAAGTTCTGGCTTTTTACCTCAGTCTCTCG TCCAACCTCCTCTCAAGTGCCCTGCGTGAGCAGCTCTGGGATCAAGGCTGGCAGACCCCGGAGACGGCGC TGATTGATGCCCACAAGCTCCTCAGAAGCATCATCATTCTGCTCATGGGAAGTGATGTGCTCTCCATTCA GAAAGCAGCCACCGAGTCGCCCTTTCTGGACTACGGTGAGAATCTGGTGCAAAAATGGCAGGTTCTCACA GAGGTGCTGACCTGCTCGGAGAGGCTGGTCGGCCGCCTGGGTCGCCTGGGGAACGTGAGTGAGGCCAAGG CCTTTTGCTTGGAGGCCCTAAAACTTACGACTAAGCTGCAGATACCTCGACAGTGTGCCCTGTTCCTTGT ACTGAAGGGTGAGCTGGAGCTGGCCCGGGGTGACATTGACCTCTGTCAGTCAGACTTGCAGCAGGTTCTC TTCTTGCTCGAGTCTTCCACAGAGTTTGGTGTTGTGACCCAGCATCCAGACTCAGTGAAGAAGGTGCACA CGCAGAAAGGGAAGCATAAGGCTCAAGGCCCATGTTTCCCACCCCTGTCAGAGGAGGAGCCCTTCCTGAA AGGTCCTGCCCTTGAGCTGGTGGACACTGTGCTCAACGAACCTGGTCCCATCCAGTCCTCTGTAAACTCC TCCCCAGTCCTGAAAACCAAGCCACCACCCAACCCTGGCTTCCTGTCTCACTTGCCCAGCTGTGACTGCT TACTGTGTGCCAGCCCTGCCCTCTCAGCAGTCTGTCTGCGCTGGGTGCTGGTCACTGCCGGAGTGAGGCT GGCCACCGGTCACAAAGCTCAGGGTCTGGATCTGCTGCAAGCTGTGCTGACGCGTTGCCCTGCAGCCACC AAGCGCTTCACACAGAGTCTCCAAGCTTCTCTGAATCACAGAACAACCCCCTCTTGTGTTCCCAGCCTCT TTGATGAGATCATGGCTCAAGTGTACACGCACTTGGCATTGGAGTTCCTGAATCAGACATCTGAAAAGAG CCTGGGGAAGGTCCTGGCGTCGGGGCTGAAGTTTGTGGCAACACGGATACAGAGCTTGGAGATCTGGAGA GCCCACCTGCTCTTGGTTCAGGCCCTTGCAAAGCTGGCCCACTTCAGCTGCTGTACTTCCGAACTCTTTG CAAGCTCCTGGGGCTGGCACCCACCATTAGTAAAAAGTCTGCCAGTCTTAGAGCCTGCTAAAATTCGGAG ACAAAAATGCTCTGGACGGGGCCGCCGGCGGATAGCCTCTGTTCCCCCACCCCTCCATAACAGCTCTCAG AAAGGTCTGGAAGAGGAAGGGCCACCTTGTACCCCTAAGCCTCCAGGTCGGGCTAGGCAAGCTGGCCCTC GTGTCCCTTTCACCATATTTGAAGAAGTCCACCCCACCAAGAGCAAGCTCCAGGTTCCCTTGGCTCCCAG GGTCCACAGGCGGGCCCAGACTCGCCTCAAGGTGATCTTCAGTGATGACAGTGACCTGGAAGACCTTGTC TCAGCTGACACACAACTTGTAGAGGAGCCTAAGAGGCGAGGGACTGCTTCCCGTACCCGGGGCCAAACTA GGAAGGGACGTAGCTTAAAGACAGATGCGGTGGTTGCTATAGAAAGTACCCCTGGACATTCCAGTGTCAG TGGGAGGACTCGGAGGGCCAGGAAGGTGGCTTCAAGAAATTGTGAAGAAGAGAGTCCCAAAGCACCCCTC TGTGTCTGGGCCAGCCAGGGACCCGAGATCATGAGAAGTATCCCTGAAGAGGAGCCAGTGGACAACCATC TGGAAAAAAGCTTCGAGATTCTCAGGGGTTCTGATGGGGAAGACTCGGCCTCAGGTGAGAAGGCGGCAGC TGCAGACACTGGTCTACCCGTAGGAGAGTGTGAGGTGTTGAGACGGGACTCCAGCAAGGCAGAGAGACCT GTCTTGTACTCAGACACGGAGGCCAATAGTGACCCTAGTCCTTGGCTCCCACCCTTCTCAGTTCCTGCAC CCATTGATCTTTCTACCCTGGATTCCATTTCTGACTCATTGAGCATTGCTTTCCGTGGTGTTAGTCACTG CCCTCCAAGTGGGCTCTATGCTCACCTCTGCCGCTTCTTGGCCCTGTGTCTGGGCCACCGAGATCCTTAT GCCACTGCGTTCCTTGTTGCTGAGTCTATCTCCATCACCTGCCGTCACCAGCTGCTCACCCACTTGCACC GACAGCTCAGCAAGGCCCAGAAGCAGCAAGAATCACCTGAACTGGCAGAGCATCTGCAGAGGCTGGACCT GAAGGAGAGGCCCGGAGGTGTCCCACTGGCCCGTATCCAGCGCCTCTTTTCCTTTAAGGCTTTGGGATCT GGCTGCTTCCCCCAGGCAGAGAAGGAGAGTTTCCAGGAGCGCCTGGCTCTGATCCCTAGTGGGGTGACTG TCTGTGTGTTGGCTCTGGCCACCCTCCAGCCTGGAACCTTGAGCAACACTCTTTTACTGACCCGCCTGGA AAAGGACAACCCCCCAATTACTGTGAAGATCCCCACTGCCCAAAATAAGCTCCCTCTAAGCGCAGTGCTG AAAGAGTTTGATGCCATCCAGAAGGACCAAAAAGAAAACAGCAGCTGTACTGAGAAACGAGTATGGTGGA CGGGCCGGCTGGCACTGGACCAAAGGATGGAGGCACTCATCACCGCCCTGGAGGAACAGGTGCTGGGCTG CTGGAGGGGGCTGCTGCTGCCATGCAGTGCTGACCCCAGCCTTGCCCAGGAGGCCTCCAAACTACAAGAG CTGCTGCGGGAATGTGGCTGGGAATATCCTGACTCCACTCTCCTGAAAGTCATCCTTAGTGGGGCCAGAA TCCTCACCAGCCAGGATGTTCAGGCCTTGGCGTGTGGGCTGTGCCCAGCCCAGCCTGACCGAGCCCAAGT GCTCCTGAGCGAGGCAGTAGGACAGGTTCAGAGCCAGGAGGCCCCTCGAAGTCAACATCTTGTATTGGTG TTAGACAAGGACCTGCAAAAGCTGCCTTGGGAAAGCACGCCCATCCTCCAAGCACAGCCGGTCACCCGGC TGCCCTCCTTCCGCTTCCTGCTCAGCTACACAGTCACTAAAGAGGCTGGAGCCTCATCAGTGCTGAGCCA AGGTGTTGATCCACAGAATACCTTCTATGTGCTAAACCCTCACAGTAACCTGTCGAGCACAGAGGAGAGA TTTCGAGCCAGTTTTAGCAGTGAAACTGGCTGGAAAGGAGTGATTGGGGAAGTGCCAAGCCTCGATCAGG TGCAGGCTGCCCTGACAGAGCGGGATTTATATATCTACGCAGGGCACGGAGCCGGTGCCCGCTTCCTTGA TGGGCAGGCTGTCCTGCGGCTGAGCTGCCGGGCGGTGGCCCTGCTGTTCGGCTGCAGCAGTGCAGCCCTT GCCGTGCATGGAAACCTGGAGGGAGCTGGTATCGTGCTCAAGTACATCATGGCTGGCTGCCCCTTGTTTC TAGGTAATCTCTGGGATGTGACTGACCGTGACATTGACCGTTACACAGAGGCTTTGCTGCAGGGCTGGCT GGGAGCAGGCCCAGGGGCTCCCTTTCTCTACTATGCCAGCCAAGCCCGCCAGGCCCCTCGACTCAAGTAC CTCATTGGTGCTGCACCTGTAGCCTACGGCCTACCTATCTCTCTGCAGACACCC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR221057 representing NM_001014976 Red=Cloning site Green=Tags(s) MRNFKGVNFATLLCSKEETQQLLPDLKEFLSRSRTDFPSSRTDAERRQICDTILRACTQQLTAKLDCPGH LRSILDLAELACDGYLLSTPQRPPLYLERILFILLRNGSTQGSPDTVLRLAQPLHACLVQNSGEAAPQDY EAVTRGSFSLFWKGAEALLERRAAFSTRLNALSFLVLLEDGSVPCEVPHFASPTACRLVAAYQLYDATGQ GLDEADADFLYEVLSRHLIRVLVGEGGSSPGPLSPQRALCLLEITLEHCRRLCWNHHHRQAARAVERARN HLEKTSVAPSLQLCQMGVELLEAVEERPGAVAQLLRKAAAVLINSIEAPSPPLRALYDSCQFFLSGLERG IRRHCGLDAILSLFAFLGGYSSLVRHLREVSEASSKQQQCLLQMHFQGFHLFTGIVYDFAQGCQATELAQ LVDGCRSAAVWMLEALEGLSGGELADYLSMTASYTSNLAYSFFSQKLYEEACVISEPVCQHLGSATSGAC PEVPPEKLHRCFRLHVESLKKLGKQAQGCKMVTLWLAALKPYSLEHMVEPVTFWVRVKMDASRAGDKELQ LQTLRDSLSCWDPETQSLLLREELRAYKSVRADTGQERFNIICDLLELSPEETAAGAWARATYLVELAQV LCYHNFTQQTNCSALDAVQEALQLLESVSPEAQEQDRLLDDKAQALLWLYICTLEAKMQEGIERDRRAQA PSNLEEFEVNDLNYEDKLQEDRFLYSSIAFNLAADAAQSKCLDQALTLWKEVLTKGRAPAVRCLQQTAAS LQILAAVYQLVAKPLQALETLLLLQIVSKRLQDHAKAASSSCQLTQLLLNLGCPSYAQLYLEEAESSLRS LDQTSDACQLLSLTCALLGSQLCWACQKVTAGVSLLLSVLRDPALQKSSKAWYLLRVQALQVLAFYLSLS SNLLSSALREQLWDQGWQTPETALIDAHKLLRSIIILLMGSDVLSIQKAATESPFLDYGENLVQKWQVLT EVLTCSERLVGRLGRLGNVSEAKAFCLEALKLTTKLQIPRQCALFLVLKGELELARGDIDLCQSDLQQVL FLLESSTEFGVVTQHPDSVKKVHTQKGKHKAQGPCFPPLSEEEPFLKGPALELVDTVLNEPGPIQSSVNS SPVLKTKPPPNPGFLSHLPSCDCLLCASPALSAVCLRWVLVTAGVRLATGHKAQGLDLLQAVLTRCPAAT KRFTQSLQASLNHRTTPSCVPSLFDEIMAQVYTHLALEFLNQTSEKSLGKVLASGLKFVATRIQSLEIWR AHLLLVQALAKLAHFSCCTSELFASSWGWHPPLVKSLPVLEPAKIRRQKCSGRGRRRIASVPPPLHNSSQ KGLEEEGPPCTPKPPGRARQAGPRVPFTIFEEVHPTKSKLQVPLAPRVHRRAQTRLKVIFSDDSDLEDLV SADTQLVEEPKRRGTASRTRGQTRKGRSLKTDAVVAIESTPGHSSVSGRTRRARKVASRNCEEESPKAPL CVWASQGPEIMRSIPEEEPVDNHLEKSFEILRGSDGEDSASGEKAAAADTGLPVGECEVLRRDSSKAERP VLYSDTEANSDPSPWLPPFSVPAPIDLSTLDSISDSLSIAFRGVSHCPPSGLYAHLCRFLALCLGHRDPY ATAFLVAESISITCRHQLLTHLHRQLSKAQKQQESPELAEHLQRLDLKERPGGVPLARIQRLFSFKALGS GCFPQAEKESFQERLALIPSGVTVCVLALATLQPGTLSNTLLLTRLEKDNPPITVKIPTAQNKLPLSAVL KEFDAIQKDQKENSSCTEKRVWWTGRLALDQRMEALITALEEQVLGCWRGLLLPCSADPSLAQEASKLQE LLRECGWEYPDSTLLKVILSGARILTSQDVQALACGLCPAQPDRAQVLLSEAVGQVQSQEAPRSQHLVLV LDKDLQKLPWESTPILQAQPVTRLPSFRFLLSYTVTKEAGASSVLSQGVDPQNTFYVLNPHSNLSSTEER FRASFSSETGWKGVIGEVPSLDQVQAALTERDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAAL AVHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGAPFLYYASQARQAPRLKY LIGAAPVAYGLPISLQTP SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001014976
ORF Size	6354 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001014976.2, NP_001014976.1
RefSeq Size	6630 bp
RefSeq ORF	6357 bp
Locus ID	105988
UniProt ID	P60330
MW	233 kDa
Gene Summary	Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms (By similarity).[UniProtKB/Swiss-Prot Function]

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